Jong Hyun Kim

KEPCO International Nuclear Graduate School, Urusan, Ulsan, South Korea

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Publications (224)460.4 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC). Here, we report a crystal structure of the MSC subcomplex comprising ArgRS, glutaminyl-tRNA synthetase (GlnRS), and the auxiliary factor aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)/p43. In this complex, the N-terminal domain of ArgRS forms a long coiled-coil structure with the N-terminal helix of AIMP1 and anchors the C-terminal core of GlnRS, thereby playing a central role in assembly of the three components. Mutation of AIMP1 destabilized the N-terminal helix of ArgRS and abrogated its catalytic activity. Mutation of the N-terminal helix of ArgRS liberated GlnRS, which is known to control cell death. This ternary complex was further anchored to AIMP2/p38 through interaction with AIMP1. These findings demonstrate the importance of interactions between the N-terminal domains of ArgRS and AIMP1 for the catalytic and noncatalytic activities of ArgRS and for the assembly of the higher-order MSC protein complex.
    Proceedings of the National Academy of Sciences of the United States of America. 10/2014;
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    ABSTRACT: Dielectric and piezoelectric properties of CuO-added KNbO3 (KN) ceramics were investigated. The CuO reacted with the Nb2O5, formed a CuO–Nb2O5-related liquid phase during the sintering, and assisted the densification of the KN ceramics at low temperatures. Moreover, some of the Cu2+ ions replaced the Nb5+ ions in the matrix and behaved as a hardener. The dielectric and piezoelectric properties of the KN ceramics were considerably influenced by the relative density. The 1.0 mol% CuO-added KN ceramic sintered at 960°C for 1.0 h, which showed a maximum relative density, exhibited a high phase angle of 86.9°, Pr of 14.8 μC/cm2, and Ec of 1.8 kV/mm. This specimen also exhibited good dielectric and piezoelectric properties: εT33/εo of 364, d33 of 122 pC/N, kp of 0.29, and Qm of 611.
    Journal of the American Ceramic Society 09/2014; · 2.43 Impact Factor
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    ABSTRACT: Adipogenesis is known to be controlled by the concerted actions of transcription factors and co-regulators. However, little is known about the regulation mechanism of transcription factors that control adipogenesis. In addition, the adipogenic roles of translational factors remain unclear. Here, we show that aminoacyl-tRNA synthetase-interacting multifunctional protein 1 (AIMP1), an auxiliary factor that is associated with a macromolecular tRNA synthetase complex, negatively regulates adipogenesis via a direct interaction with the DNA-binding domain of peroxisome proliferator-activated receptor γ (PPARγ). AIMP1 expression increased during adipocyte differentiation. Adipogenesis was augmented in AIMP1-deficient cells, as compared with control cells. AIMP1 exhibited high affinity for active PPARγ and interacted with the DNA-binding domain of PPARγ, thereby inhibiting its transcriptional activity. Thus, AIMP1 appears to function as a novel inhibitor of PPARγ that regulates adipocyte differentiation by preventing the transcriptional activation of PPARγ.
    Journal of cell science. 08/2014;
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    ABSTRACT: Since automation was introduced in various industrial fields, the concept of the automation rate has been used to indicate the inclusion proportion of automation among all work processes or facilities. Expressions of the inclusion proportion of automation are predictable, as is the ability to express the degree of the enhancement of human performance. However, many researchers have found that a high automation rate does not guarantee high performance. Therefore, to reflect the effects of automation on human performance, this paper proposes a new estimation method of the automation rate that considers the effects of automation on human operators in nuclear power plants (NPPs). Automation in NPPs can be divided into two types: system automation and cognitive automation. Some general descriptions and characteristics of each type of automation are provided, and the advantages of automation are investigated. The advantages of each type of automation are used as measures of the estimation method of the automation rate. One advantage was found to be a reduction in the number of tasks, and another was a reduction in human cognitive task loads. The system and the cognitive automation rate were proposed as quantitative measures by taking advantage of the aforementioned benefits. To quantify the required human cognitive task loads and thus suggest the cognitive automation rate, Conant’s information-theory-based model was applied. The validity of the suggested method, especially as regards the cognitive automation rate, was proven by conducting experiments. The result showed that a decreased rate of the operator working time was significantly related to the cognitive automation rate and that the calculation of the cognitive task load was useful as a measure of the cognitive automation rate.
    Annals of Nuclear Energy 08/2014; 70:48–55. · 0.80 Impact Factor
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    ABSTRACT: At present, gold-standard technique of cervical cord decompression is surgical decompression and fusion. But, many complications related cervical fusion have been reported. We adopted an extended anterior cervical foraminotomy (EACF) technique to decompress the anterolateral portion of cervical cord and report clinical results and effectiveness of this procedure.
    Journal of Korean Neurosurgical Society. 08/2014; 56(2):114-20.
  • Neurosurgery Quarterly 07/2014; · 0.09 Impact Factor
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    ABSTRACT: The reported mortality rates range from 28% to 100% in burn patients who develop acute kidney injury (AKI) and from 50% to 100% among such patients treated with renal replacement therapy. Recently, the serum cystatin C and plasma and urine neutrophil gelatinase-associated lipocalin (NGAL) levels have been introduced as early biomarkers for AKI; the levels of these biomarkers are known to increase 24 to 48 hours before the serum creatinine levels increase. In this study, we aimed to estimate the diagnostic utility of the cystatin C and plasma and urine NGAL levels in the early post-burn period as biomarkers for predicting AKI and mortality in patients with major burn injuries.
    Critical care (London, England) 07/2014; 18(4):R151. · 4.72 Impact Factor
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    ABSTRACT: To determine epidemiological trends among burns patients admitted to our burns center during 2003-2012, and the usefulness of the Abbreviated Burns Severity Index (ABSI) for predicting burns-related mortality.
    Burns: journal of the International Society for Burn Injuries 07/2014; · 1.95 Impact Factor
  • International journal of cardiology 04/2014; · 6.18 Impact Factor
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    ABSTRACT: Ganglioglioma is an infrequent tumor of the central nervous system (CNS); mostly supratentorial region. But, they can occur anywhere in the central nervous system such as brainstem, cerebellopontine angle (CPA), thalamus, optic nerve and spinal cord. Although it occurs rarely, ganglioglioma should be included in the differential diagnosis of a posterior fossa mass because early recognition is important for treatment and patient counseling.
    Journal of Korean Neurosurgical Society. 03/2014; 55(3):164-6.
  • Jong Hyun Kim, Suk Sang Chang, Geunbae Lim
    Current Applied Physics 01/2014; · 2.03 Impact Factor
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    Jong Hyun Kim, Suk Sang Chang, Geunbae Lim
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    ABSTRACT: Conventional machining technologies such as a milling process have limitations in accuracy to fabricate microstructures. Deep X-ray lithography using the synchrotron radiation is a promising micromachining process with an excellent accuracy, whereas there are difficulties in the fabrication of multi-layered structures. Therefore, it is mainly used for fabricating simple mono-layered microstructures with a high aspect ratio. In this study, a novel technology for fabricating multi-layered microstructures is proposed by combining two processes. In advance, an X-ray resist material is cut and machined into various shapes and heights by the micro milling process. Subsequent X-ray irradiation process facilitates the fabrication of multi-layered microstructures. The proposed technology can overcome the limitation of the pattern accuracy in conventional milling process and the difficulty of the multi-layered machining in x-ray process. The usefulness of the proposed technology is demonstrated in this study by applying the technique in the realization of various multi-layered microstructures.
    Journal of the Korean Society for Precision Engineering. 01/2014; 31(3).
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    ABSTRACT: Objective To evaluate the effect of burn rehabilitation massage therapy on hypertrophic scar after burn. Method One hundred and forty-six burn patients with hypertrophic scar(s) were randomly divided into an experimental group and a control group. All patients received standard rehabilitation therapy for hypertrophic scars and 76 patients (massage group) additionally received burn scar rehabilitation massage therapy. Both before and after the treatment, we determined the scores of visual analog scale (VAS) and itching scale and assessed the scar characteristics of thickness, melanin, erythema, transepidermal water loss (TEWL), sebum, and elasticity by using ultrasonography, Mexameter®, Tewameter®, Sebumeter®, and Cutometer®, respectively. Results The scores of both VAS and itching scale decreased significantly in both groups, indicating a significant intragroup difference. With regard to the scar characteristics, the massage group showed a significant decrease after treatment in scar thickness, melanin, erythema, TEWL and a significant intergroup difference. In terms of scar elasticity, a significant intergroup difference was noted in immediate distension and gross skin elasticity, while the massage group significant improvement in skin distensibility, immediate distension, immediate retraction, and delayed distension. Conclusion Our results suggest that burn rehabilitation massage therapy is effective in improving pain, pruritus, and scar characteristics in hypertrophic scars after burn.
    Burns: journal of the International Society for Burn Injuries 01/2014; · 1.95 Impact Factor
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    ABSTRACT: Aminoacyl-tRNA synthetases recognize cognate amino acids and tRNAs from their noncognate counterparts and catalyze the formation of aminoacyl-tRNAs. Halofuginone (HF), a coccidiostat used in veterinary medicine, exerts its effects by acting as a high-affinity inhibitor of the enzyme glutamyl-prolyl-tRNA synthetase (EPRS). In order to elucidate the precise molecular basis of this inhibition mechanism of human EPRS, the crystal structures of the prolyl-tRNA synthetase domain of human EPRS (hPRS) at 2.4 Å resolution (hPRS-apo), of hPRS complexed with ATP and the substrate proline at 2.3 Å resolution (hPRS-sub) and of hPRS complexed with HF at 2.62 Å resolution (hPRS-HF) are presented. These structures show plainly that motif 1 functions as a cap in hPRS, which is loosely opened in hPRS-apo, tightly closed in hPRS-sub and incorrectly closed in hPRS-HF. In addition, the structural analyses are consistent with more effective binding of hPRS to HF with ATP. Mutagenesis and biochemical analysis confirmed the key roles of two residues, Phe1097 and Arg1152, in the HF inhibition mechanism. These structures will lead to the development of more potent and selective hPRS inhibitors for promoting inflammatory resolution.
    Acta Crystallographica Section D Biological Crystallography 10/2013; 69(Pt 10):2136-2145. · 12.67 Impact Factor
  • Jong Hyun Kim, Jung Min Han, Sunghoon Kim
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    ABSTRACT: Protein-protein interaction occurs transiently or stably when two or more proteins bind together to mediate a wide range of cellular processes such as protein modification, signal transduction, protein trafficking, and structural folding. The macromolecules involved in protein biosynthesis such as aminoacyl-tRNA synthetase (ARS) have a number of protein-protein interactions. The mammalian multi-tRNA synthetase complex (MSC) consists of eight different enzymes: EPRS, IRS, LRS, QRS, MRS, KRS, RRS, and DRS, and three auxiliary proteins: AIMP1/p43, AIMP2/p38, and AIMP/p18. The distinct ARS proteins are also connected to diverse protein networks to carry out biological functions. In this chapter we first show the protein networks of the entire MSC and explain how MSC components interact with or can regulate other proteins. Finally, it is pointed out that the understanding of protein-protein interaction mechanism will provide insight to potential therapeutic application for diseases related to the MSC network.
    Topics in current chemistry 09/2013; · 8.46 Impact Factor
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    ABSTRACT: Background: The purpose of this study is to clarify the clinical significance of serum transthyretin (TTR) in massively burned patients in nutrition support and clinical severity. Methods: A retrospective study was performed with 204 patients admitted to Hangang Sacred Heart Hospital's Burn Center September 2010-September 2012 with burn wounds > 20% of total body surface area (TBSA) burned. Serum TTR, C-reactive protein (CRP), lactic acid, and an NST index (calculated by dividing real caloric intake by estimated caloric need for 7 days) were analyzed on a weekly basis for 5 weeks after admission. Results: When we classified patients with severity according to mortality, percentage of TBSA burned, serum lactic acid, and CRP, mean serum TTR level was significantly higher in the less severe patient group than in the severe patient group in each week for every severity index. And the serum TTR level did not show significant differences by NST index in both the severe patient group and the less severe patient group. In a multivariate logistic regression, percentage TBSA burned, TTR, and lactic acid had an independent association with mortality. Conclusion: Serum TTR did not show a significant difference by nutrition support in massively burned patients with >20% of TBSA burned. But serum TTR was significantly different by the severity of the patient and independently associated with mortality. Hence, serum TTR level can be 1 of the useful biomarkers for comprehending the severity of the illness in massively burned patients.
    Journal of Parenteral and Enteral Nutrition 08/2013; · 2.49 Impact Factor
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    ABSTRACT: Wilson disease (WD) is an autosomal recessive disorder of copper transport caused by alteration of the adenosine triphosphatase 7B gene. It is rare to diagnose WD below the age of three years. Molecular genetic testing is one of the most important diagnostic methods and may confirm the diagnosis in equivocal cases. We report a case of a 9-mo old boy with WD who presented as chronic hepatitis. Genetic analysis showed compound heterozygotes of p.G1186S and c.4006delA.
    World journal of hepatology. 03/2013; 5(3):156-9.
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    ABSTRACT: Rotavirus gastroenteritis is the leading cause of severe acute gastroenteritis in children worldwide and is associated with high hospitalization and mortality rates in children younger than 5 years of age. Vaccination is necessary to prevent rotavirus infection. Two live attenuated and orally administered rotavirus vaccines became commercially available in Korea. The aim of this study is to describe epidemiological changes in rotavirus gastroenteritis after the introduction of rotavirus vaccines in Korea. The medical records of 11,199 children younger than 5 years of age and hospitalized for acute gastroenteritis from August 2007 to July 2010 in eight Korean hospitals were reviewed. Rotavirus was detected in stool samples obtained from 2,959 children (26.42 %). The authors evaluated the percentage of rotavirus gastroenteritis among all acute gastroenteritis hospitalizations in eight hospitals located in different geographical areas and analyzed epidemiological changes in rotavirus gastroenteritis according to age, geographical area, and season. According to the findings, the percentage of rotavirus gastroenteritis showed a decrease in children eligible for vaccination during the study period. After introduction of the vaccine, reduced rates of rotavirus detection were observed in all of the geographical areas, and the greatest reduction was observed in Seoul. In Seoul, there was a marked delay of the rotavirus season. Conclusion: Epidemiologic changes in Korea after the introduction of rotavirus vaccine are consistent with changes observed in other countries.
    European Journal of Pediatrics 02/2013; · 1.98 Impact Factor
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    ABSTRACT: This study was carried out to investigate the effects of bamboo charcoal and bamboo vinegar as alternatives of antibiotics in the diet of fattening pigs and their influence on growth performance, immune responses and fecal microflora populations. Crossed pigs (n = 144, 79 kg body weight) were divided into 12 heads per pen, four diets and three replications. The basal diet (negative control: NC) was supplemented with 0.3% antibiotics (positive control: PC), 0.3% bamboo charcoal (BC) and 0.3% bamboo vinegar (BV). Average daily weight gain and feed efficiency were higher (P < 0.05) in PC, BC and BV. The concentration of lactate dehydrogenase and cortisol were lower (P < 0.05), but the concentration of immunoglobulin G (IgG) and IgA were higher (P < 0.05) in PC, BC and BV. Counts of coliform bacteria and Salmonella spp. were lower (P < 0.05), while the counts of fecal anaerobic total bacteria and lactic acid bacteria were higher (P < 0.05) in PC, BC and BV. A reasonable inclusion of bamboo charcoal or bamboo vinegar as antibiotics in the diet of fattening pigs leads to a better growth performance, immune responses and fecal microflora populations. The results of the present study suggest that bamboo charcoal or bamboo vinegar could be a potential additives in animal production as an alternative to antibiotics.
    Animal Science Journal 02/2013; 84(2):113-20. · 1.04 Impact Factor
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    ABSTRACT: AMP-activated protein kinase has been described as a key signaling protein that can regulate energy homeostasis. Here, we aimed to characterize novel AMPK activating compounds that have a much lower effective concentration than metformin. As a result, Emodin, a natural anthraquinone derivative was shown to stimulate AMPK activity in skeletal muscle and liver cell. Emodin enhanced GLUT4 translocation and [14C] glucose uptake into myotube in an AMPK dependent manner. Also, emodin inhibited glucose production by suppressing the expression of key gluconeogenic genes, such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) in hepatocytes. Furthermore, we found that emodin can activate AMPK by inhibiting mitochondrial respiratory complex I activity, leading to increased reactive oxygen species (ROS) and Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) activity. Finally, we confirmed that a single-dose administration of emodin significantly decreased the fasting plasma glucose levels and improved glucose tolerance in C57Bl/6J mice. Increased insulin sensitivity was also confirmed after daily injection of emodin for eight days using an insulin tolerance test and insulin stimulated PI-3K phosphorylation in wild type and HFD induced diabetic mouse models. Our study suggests that emodin regulates glucose homeostasis in vivo by AMPK activation and that this may represent a novel therapeutic principle in the treatment of Type 2 diabetic models.
    Journal of Biological Chemistry 01/2013; · 4.65 Impact Factor

Publication Stats

1k Citations
460.40 Total Impact Points


  • 2014
    • KEPCO International Nuclear Graduate School
      Urusan, Ulsan, South Korea
  • 2013–2014
    • Seoul National University
      • • Department of Molecular Medicine and Biopharmaceutical Sciences (WCU)
      • • Medicinal Bioconvergence Research Center
      Sŏul, Seoul, South Korea
    • Gyeongnam National University of Science and Technology
      • Department of Animal Resources Technology (Graduate School)
      Sŏul, Seoul, South Korea
  • 2012–2014
    • Konyang University Hospital
      Gaigeturi, Jeju, South Korea
    • Gwangju Institute of Science and Technology
      • School of Mechatronics
      Gwangju, Gwangju, South Korea
    • National Institutes of Health
      • Laboratory of Molecular Biology
      Bethesda, MD, United States
    • Sapporo Higashi Tokushukai Hospital
      Sapporo, Hokkaidō, Japan
    • National Fisheries Research and Development Institution
      Sŏul, Seoul, South Korea
  • 2003–2014
    • Korea University
      • • Department of Materials Science and Engineering
      • • Department of Chemistry
      Sŏul, Seoul, South Korea
  • 2002–2014
    • Pohang University of Science and Technology
      • • Department of Mechanical Engineering
      • • Division of Molecular and Life Sciences
      Geijitsu, North Gyeongsang, South Korea
    • Seoul National University Hospital
      • Department of Ophthalmology
      Seoul, Seoul, South Korea
    • Kumoh National Institute of Technology
      Sŏul, Seoul, South Korea
  • 2011–2013
    • Gyeongsang National University
      • • College of Veterinary Medicine
      • • Department of Neurosurgery
      Chinju, South Gyeongsang, South Korea
    • Sogang University
      • Department of Mechanical Engineering
      Sŏul, Seoul, South Korea
    • National Heart, Lung, and Blood Institute
      Maryland, United States
  • 2009–2013
    • Hallym University Medical Center
      • Department of Burn Surgery
      Sŏul, Seoul, South Korea
    • Catholic University of Korea
      • Department of Pediatrics
      Sŏul, Seoul, South Korea
    • Chungnam National University
      • Department of Physics
      Seongnam, Gyeonggi, South Korea
  • 2010–2012
    • Hannam University
      Daiden, Daejeon, South Korea
    • National Institute of Health, Korea
      • Division of Enteric and Hepatitis Viruses
      Cheongsong gun, North Gyeongsang, South Korea
    • Samsung Medical Center
      • Department of Neurosurgery
      Seoul, Seoul, South Korea
  • 2008–2012
    • Chonnam National University
      • Department of Internal Medicine
      Gwangju, Gwangju, South Korea
    • Pohang Accelerator Laboratory
      Urusan, Ulsan, South Korea
    • Korea Hydro and Nuclear Power - Central Research Institute
      Sŏul, Seoul, South Korea
    • National Cancer Center Korea
      Kōyō, Gyeonggi Province, South Korea
  • 2002–2012
    • Sungkyunkwan University
      • • Department of Neurosurgery
      • • Department of Radiology
      Sŏul, Seoul, South Korea
  • 2009–2011
    • Harvard Medical School
      • Department of Genetics
      Boston, MA, United States
    • Chonnam National University Hospital
      Sŏul, Seoul, South Korea
  • 2008–2010
    • Hallym University
      • College of Medicine
      Sŏul, Seoul, South Korea
  • 1993–2010
    • Korea Advanced Institute of Science and Technology
      • • Department of Chemical and Biomolecular Engineering
      • • Department of Nuclear and Quantum Engineering
      • • Department of Physics
      Sŏul, Seoul, South Korea
  • 2007
    • Yonsei University
      • Department of Computer Science
      Seoul, Seoul, South Korea
    • University of Southern California
      • Department of Computer Science
      Los Angeles, CA, United States
  • 2005
    • Showa University
      • Institute of Molecular Oncology
      Shinagawa, Tōkyō, Japan
  • 1996
    • Pusan National University
      Tsau-liang-hai, Busan, South Korea
  • 1995
    • Dongseo University
      • Department of Bio-chemical Engineering
      Tsau-liang-hai, Busan, South Korea