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ABSTRACT: To study the expression and prognostic significance of cyclooxygenase-2 (Cox-2) in supraglottic laryngeal squamous cell carcinomas (SGLSCC) and identify the relationship between Cox-2 and angiogenesis and the roles of Cox-2 in SGLSCC as a biological marker.
Eighty-eight primary SGLSCC patients received surgical treatment were studied by immunohistochemical staining, and reverse transcription-polymerase chain reaction (RT-PCR) technique.
The percentage of Cox-2-positive cells was 94.3% (83/88) in SGLSCC whereas there was no immunostaining in the all cells of normal mucosa of paracarcinoma. Cox-2 expression was higher in well-differentiated tumors compared with poorly-differentiated SGLSCC. The relative concentration of Cox-2 mRNA was 141.871 +/- 20.5435 in SGLSCC and 17.031 +/- 2.2597 in normal paracarcinoma mucosa (P < 0.01). It was significantly higher in SGLSCC than in normal paracarcinoma mucosa. In SGLSCC, only pathological grading and the percentage of Cox-2-positive cells had significant correlation (P < 0.01). And not only the percentage of Cox-2-positive cells but also Cox-2 intensity had significant correlation with microvessel density (MVD) (P < 0.01). Kaplan-Meier survival analysis showed there had significant relationship between Cox-2 intensity and cumulative survival rate of SGLSCC patients (P < 0.05). But the percentage of Cox-2-positive cells was different (P > 0.05). Cox's regression analysis indicated that Cox-2 intensity were significantly independent prognostic factors (P < 0.01).
Cox-2 expression maybe relate to the carcinogenesis and progress in tumors especially in well-differentiated ones; The changes of Cox-2 expression are synchronous with MVD. Cox-2 intensity is a significantly independent prognostic factor. So Cox-2 may be an effective target of prevention, therapy and prognostic evaluation for laryngeal carcinoma and other head and neck squamous cell carcinomas (HNSCC).
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 01/2008; 21(24):1109-13.