Jian Li

Southwest University for Nationalities, Hua-yang, Sichuan, China

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Publications (90)231.96 Total impact

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    ABSTRACT: Hypoxia-inducible factors (HIFs) are oxygen-dependent transcriptional activators, which play crucial roles in tumor angiogenesis and mammalian development, and regulate the transcription of genes involved in oxygen homeostasis in response to hypoxia. However, information on HIF-1α and HIF-2α in yak (Bos grunniens) is scarce. The complete coding region of yak HIF-2α was cloned, its mRNA expression in several tissues were determined, and the expression levels were compared with those of closely related low-altitude cattle (Bos taurus), and the methylation status of promoter regions were analyzed to better understand the roles of HIF-1α and HIF-2α in domesticated yak. The yak HIF-2α cDNA was cloned and sequenced in the present work reveals the evolutionary conservation through multiple sequence alignment, although 15 bases changed, resulting in 8 amino acid substitutions in the translated proteins in cattle. The tissue-specific expression results showed that HIF-1α is ubiquitously expressed, whereas HIF-2α expression is limited to endothelial tissues (kidney, heart, lung, spleen, and liver) and blood in yak. Both HIF-1α and HIF-2α expressions were higher in yak tissues than in cattle. The HIF-1α expression level is much higher in yak than cattle in these organs, except for the lung (P < 0.05), but the HIF-2α gene is significantly different in the heart, spleen, and kidney (P < 0.05). Furthermore, the methylation levels in the 5' flanking regulatory regions of HIF-1α and HIF-2α in yak kidney were significantly decreased than cattle counterparts (P < 0.05). Identifying these genes and the comparison of different expressions facilitates the understanding of the biological high-altitude hypoxic stress response mechanism and may assist current medical research to understand hypoxia-related diseases.
    Animal Biotechnology 07/2015; 26(3):222-9. DOI:10.1080/10495398.2014.1002563 · 0.64 Impact Factor
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    ABSTRACT: The electronic structure of eight zinc-centered porphyrin macrocyclic molecules are investigated using density functional theory for ground-state properties, time-dependent density functional theory (TDDFT) for excited states, and Franck-Condon (FC) analysis for further characterization of the UV-vis spectrum. Symmetry breaking was utilized to find the lowest energy of the excited states for many states in the spectra. To confirm the theoretical modeling, the spectroscopic result from zinc phthalocyanine (ZnPc) is used to compare to the TDDFT and FC result. After confirmation of the modeling, five more planar molecules are investigated: zinc tetrabenzoporphyrin (ZnTBP), zinc tetrabenzomonoazaporphyrin (ZnTBMAP), zinc tetrabenzocisdiazaporphyrin (ZnTBcisDAP), zinc tetrabenzotransdiazaporphyrin (ZnTBtransDAP), and zinc tetrabenzotriazaporphyrin (ZnTBTrAP). The two latter molecules are then compared to their phenylated sister molecules: zinc monophenyltetrabenzotriazaporphyrin (ZnMPTBTrAP) and zinc diphenyltetrabenzotransdiazaporphyrin (ZnDPTBtransDAP). The spectroscopic results from the synthesis of ZnMPTBTrAP and ZnDPTBtransDAP are then compared to their theoretical models and non-phenylated pairs. While the Franck-Condon results were not as illuminating for every B-band, the Q-band results were successful in all eight molecules, with a considerable amount of spectral analysis in the range of interest between 300 and 750 nm. The π-π(∗) transitions are evident in the results for all of the Q bands, while satellite vibrations are also visible in the spectra. In particular, this investigation finds that, while ZnPc has a D4h symmetry at ground state, a C4v symmetry is predicted in the excited-state Q band region. The theoretical results for ZnPc found an excitation energy at the Q-band 0-0 transition of 1.88 eV in vacuum, which is in remarkable agreement with published gas-phase spectroscopy, as well as our own results of ZnPc in solution with Tetrahydrofuran that are provided in this paper.
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    ABSTRACT: Highly efficient and stable palladium complexes which exhibit both phosphorescence and delayed fluorescence are developed. We demonstrate that the emission from the two processes can be separately tuned through rational ligand modification. External quantum efficiencies over 20% are achieved and stable devices demonstrate an operational lifetime to 90% initial luminance estimated at over 20 000 h at 100 cd m(-2) . © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Advanced Materials 03/2015; 27(15). DOI:10.1002/adma.201401772 · 15.41 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the effects of Lactobacillus fermentum Lee (LF-Lee) on activated carbon-induced constipation in ICR mice. ICR mice were orally administered lactic acid bacteria for nine days. Body weight, dietary and water intake, defecation status, gastrointestinal (GI) transit and defecation time, as well as levels of motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) in serum were measured to evaluate the preventive effects of LF-Lee on constipation. Bisacodyl, a laxative drug, was administered as a positive control. The time taken until the first defecation of a black stool for normal, control, bisacodyl- (100 mg/kg, oral administration), Lactobacillus bulgaricus (LB)-, LF-Lee low dose (L)- and LF-Lee high dose (H)-treated mice was 90, 218, 117, 180, 161 and 151 min, respectively. Following the consumption of LB, LF-Lee (L) or LF-Lee (H), or the oral administration of bisacodyl, the GI transit was reduced to 55.2, 65.8, 73.1 and 94.6%, respectively, of the transit in normal mice. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and those of SS were reduced in the mice treated with LF-Lee compared with those in the untreated control mice (P<0.05). These results demonstrate that lactic acid bacteria have preventive effects on constipation in mice and that LF-Lee has superior functional activity.
    Experimental and therapeutic medicine 01/2015; 9(1):272-278. DOI:10.3892/etm.2014.2064 · 0.94 Impact Factor
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    ABSTRACT: Insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-1 (IGFBP-1) play a pivotal role in regulating cellular hypoxic response. In this study, we cloned and characterized the genes encoding IGF-1 and IGFBP-1 to improve the current knowledge on their roles in highland Bos grunniens (Yak). We also compared their expression levels in the liver and kidney tissues between yaks and lowland cattle. We obtained full-length 465 bp IGF-1 and 792 bp IGFBP-1, encoding 154 amino acids (AA) IGF-1, and 263 AA IGFBP-1 protein, respectively using reverse transcriptase-polyerase chain reaction (RT-PCR) technology. Analysis of their corresponding amino acid sequences showed a high identity between B. grunniens and lowland mammals. Moreover, the two genes were proved to be widely distributed in the examined tissues through expression pattern analysis. Real-time PCR results revealed that IGF-1 expression was higher in the liver and kidney tissues in B. grunniens than in Bos taurus (p<0.05). The IGFBP-1 gene was expressed at a higher level in the liver (p<0.05) of B. taurus than B. grunniens, but it has a similar expression level in the kidneys of the two species. These results indicated that upregulated IGF-1 and downregulated IGFBP-1 are associated with hypoxia adaptive response in B. grunniens.
    Asian Australasian Journal of Animal Sciences 01/2015; 28(1):20-4. DOI:10.5713/ajas.14.0413 · 0.56 Impact Factor
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    ABSTRACT: This study aimed to establish yak mammary epithelial cells (YMECs) for an in vitro model of yak mammary gland biology. The primary culture of YMECs was obtained from mammary gland tissues of lactating yak and then characterized using immunocytochemistry, RT-PCR, and western blot analysis. Whether foreign genes could be transfected into the YMECs were examined by transfecting the EGFP gene into the cells. Finally, the effect of Staphylococcus aureus infection on YMECs was determined. The established YMECs retained the mammary epithelial cell characteristics. A spontaneously immortalized yak mammary epithelial cell line was established and could be continuously subcultured for more than 60 passages without senescence. The EGFP gene was successfully transferred into the YMECs, and the transfected cells could be maintained for a long duration in the culture by continuous subculturing. The cells expressed more antimicrobial peptides upon S.aureus invasion. Therefore, the established cell line could be considered a model system to understand yak mammary gland biology.
    PLoS ONE 12/2014; 9(12):e113669. DOI:10.1371/journal.pone.0113669 · 3.53 Impact Factor
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    ABSTRACT: Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone ERα and ERβ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of ERα and ERβ. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak ERα and ERβ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that ERα and ERβ were expressed in a variety of tissues, but the expression level of ERα was higher than that of ERβ in all tissues, except testis. The mRNA expression of ERα was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The ERβ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.
    Asian Australasian Journal of Animal Sciences 12/2014; 27(12):1684-90. DOI:10.5713/ajas.2014.14380 · 0.56 Impact Factor
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    ABSTRACT: In order to develop organic light-emitting diodes with improved optical properties, a series of phosphorescent complexes exhibiting narrow-band emission spectra are prepared and color tuned to emit efficiently across the whole visible spectrum through a judicious molecular design. Devices employing a green narrow-band phosphorescent emitter are fabricated and demonstrate an internal quantum efficiency of close to unity and impressive device operational lifetimes, estimate at over 70 000 h at a practical luminance of 100 cd m-2. Additionally, a deep blue narrow-band emitter is incorporated into a device setting that demonstrates a peak external quantum efficiency of 17.6% and CIE coordinates of (0.14, 0.09).
    12/2014; 3(3). DOI:10.1002/adom.201400341
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    ABSTRACT: The aim of this study was to investigate the effects of Lactobacillus fermentum Suo (LF-Suo) on activated carbon-induced constipation in ICR (Institute of Cancer Research) mice. ICR mice were orally administered with lactic acid bacteria for 9 days. Body weight, diet intake, drinking amount, defecation status, gastrointestinal transit and defecation time, and the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), SS (somatostatin), AChE (acetylcholinesterase), SP (substance P), VIP (vasoactive intestinal peptide) were used to evaluate the preventive effects of LF-Suo on constipation. Bisacodyl, a laxative drug, was used as a positive control. The normal, control, 100 mg/kg bisacodyl treatment, LB (Lactobacillus bulgaricus)-, LF-Suo (L)- and LF-Suo (H)-treated mice showed the time to the first black stool defecation at 90, 218, 117, 180, 155 and 137 min, respectively. By the oral administration of LB-, LF-Suo (L), LF-Suo (H) or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 55.2%, 72.3%, 85.5% and 94.6%, respectively, of the transit in normal mice, respectively. In contrast to the control mice, the serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Suo (p < 0.05). By the RT-PCR (reverse transcription-polymerase chain reaction) and western blot assays, LF-Suo increased the c-Kit, SCF (stem cell factor), GDNF (glial cell line-derived neurotrophic factor) and decreased TRPV1 (transient receptor potential vanilloid 1), NOS (nitric oxide synthase) expressions of small intestine tissue in mice. These results demonstrate that lactic acid bacteria has preventive effects on mouse constipation and LF-Suo demonstrated the best functional activity.
    International Journal of Molecular Sciences 12/2014; 15(12):21875-95. DOI:10.3390/ijms151221875 · 2.46 Impact Factor
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    ABSTRACT: Halogenated solvents such as dichlorobenzene (DCB) are commonly used in the fabrication of bulk-heterojunction (BHJ) organic solar cells. However, most halogenated solvents are very toxic. This report provides a route to eliminate halogenated solvents based on Hansen Solubility Parameters (HSPs) for manufacturing high efficiency BHJ solar cells. The device performances fabricated from several different binary solvent systems were compared to that from DCB. For the investigation of different gel behavior of solvent mixtures, Hansen solubility parameters and viscosity were mainly considered for the selection of binary solvent mixture candidates. Upon the addition of 20 vol.% benzaldehyde (BA) to p-xylene (p-XL), the device performances were improved in power conversion efficiency (PCE) from 3.1% to 3.8% and external quantum efficiency (EQE) from 63% to 70%. The solar cell devices fabricated using p-XL/BA binary solvent mixtures exhibited PCE comparable to that from DCB.
    Organic Electronics 10/2014; DOI:10.1016/j.orgel.2014.10.025 · 3.68 Impact Factor
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    ABSTRACT: A series of tetradentate platinum complexes that exhibit both efficient monomer and excimer emission are synthesized. Via small modifications to the cyclometalating ligands, both the monomer and excimer emission energy can be separately tuned. Devices employing all of the developed emitters demonstrate impressively high external quantum efficiencies (EQEs) within the range of 22% to 27% for concentrations between 2% and 16%. The halogen-free design of the complexes also enables the fabrication of single, doped, white organic light-emitting diodes (OLEDs) with long operational lifetimes. A balanced white device employing the complex Pt2O2, achieves a device operational lifetime to 80% of the initial luminance estimated at over 200 h at 1000 cd m–2, while also achieving 12.5% peak EQE for a warm white light with a color rendering index of 80. Furthermore, a highly doped device exhibiting nearly exclusive excimer emission showed an impressive operational lifetime, which is estimated at more than 400 h for 1000 cd m-2.
    Advanced Functional Materials 10/2014; 24(38). DOI:10.1002/adfm.201401244 · 10.44 Impact Factor
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    ABSTRACT: We propose an optical sideband processing approach for phase-modulation direct-detection microwave photonics link in this paper. With optical sideband processing, phase modulation signal is converted to intensity modulation signal for direct detection and nonlinearity compensation is realized to suppress third-order intermodulation distortion simultaneously. Theoretical analysis shows that, by imposing proper phase shifts on zero and second-order optical sidebands, corresponding third-order intermodulation distortion components can cancel each other. A proof-of-concept experiment is carried out for verification. Experimental results demonstrate a 30.06 dB suppression of third-order intermodulation distortion and the linearized link's dynamic range can also be improved by 17.42 dB compared with the nonlinearized link, which verify well a highly linearized phase-modulation direct-detection microwave photonics link using this proposal.
    IEEE Photonics Journal 10/2014; 6(5):1-10. DOI:10.1109/JPHOT.2014.2360293 · 2.33 Impact Factor
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    ABSTRACT: We demonstrate the power conversion efficiency of bulk heterojunction organic solar cells can be enhanced by introducing Ag nanoparticles into organic exciton blocking layer. The Ag nanoparticles were incorporated into the exciton blocking layer by thermal evaporation. Compared with the conventional cathode contact materials such as Al, LiF/Al, devices with Ag nanoparticles incorporated in the exciton blocking layer showed lower series resistances and higher fill factors, leading to a 3.2% power conversion efficiency with a 60 nm active layer; whereas, the conventional devices have only 2.0-2.3% power conversion efficiency. Localized surface plasmon resonances by the Ag nanoparticles and their contribution to photocurrent were also discussed by simulating optical absorptions using a FDTD (finite-difference-time-domain) method.
    Organic Electronics 10/2014; 15(10):2414–2419. DOI:10.1016/j.orgel.2014.06.020 · 3.68 Impact Factor
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    ABSTRACT: Efficient deep-blue-emitting tetradentate platinum complexes with a narrow spectral bandwidth are presented, which demonstrate CIEx ≈ 0.15 and CIEy < 0.1. Ultimately, an organic light-emitting diode (OLED) with 24.8% peak external quantum efficiency and CIE coordinates of (0.147, 0.079) is fabricated using PtON7-dtb.
    Advanced Materials 09/2014; 26(41). DOI:10.1002/adma.201401759 · 15.41 Impact Factor
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    ABSTRACT: We propose a novel photonic downconversion method based on optical carrier reusing. In the proposed system, the phase modulator (PM) is placed between two narrowband fiber Bragg gratings (FBGs), which are designed to reflect the optical carrier and transmit the optical sidebands. The optical carrier is modulated in the PM in two directions and is not injected into the photodetector (PD). Thus, the utilization ratio of the optical carrier is enhanced and the power saturation in the PD is avoided. Consequently, the system gain can be improved. In addition, a 2×2 optical coupler is cooperated with a balanced PD behind these two FBGs. Resulting from the subtle design of the phase difference, gain is furthermore doubled and intensity noise can be reduced simultaneously. In the experiments, 29 dB gain improvement is measured compared with the traditional dual-series intensity modulators method. The noise floor is reduced by 12.1 dB.
    Optics Letters 09/2014; 39(17). DOI:10.1364/OL.39.004990 · 3.18 Impact Factor
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    ABSTRACT: Oligoacene materials were applied as anode interfacial layers for small molecular organic photovoltaics. A sizeable enhancement in the short circuit current was observed which is attributed to an enhancement in the exciton diffusion efficiency. The application of tetracene anode interfacial layers to a synthesized ZnPc material yielded maximum power conversion efficiencies of 4.7%. Rubrene was also applied as an anode interfacial layer for devices of SubPc/C60 which showed a similar photocurrent enhancement.
    2014 21st International Workshop on Active-Matrix Flatpanel Displays and Devices (AM-FPD); 07/2014
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    ABSTRACT: RNA-Seq, a high-throughput (HT) sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After Illumina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5' or 3' ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200?4933 mapped to the non-redundant (nr), nucleotide (nt) and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus (41.4%), Bos grunniens mutus (33.0%), Ovis aries (6.3%), Homo sapiens (2.8%), Mus musculus (1.6%) and other species. Functional analysis showed that these expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly advantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.
    Science China. Life sciences 06/2014; 57(9). DOI:10.1007/s11427-014-4678-2 · 1.51 Impact Factor
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    ABSTRACT: We cloned and sequenced four pivotal cDNAs involved in DNA structural maintenance (H1F0 and TOP1) and the cell cycle (CLTA and CDK1) from yak oocytes. In addition, we studied the consequences of freezing-thawing (F/T) processes on the expression of their mRNA transcripts in yak immature and in vitro matured (MII) oocytes.
    Cryobiology 05/2014; 69(1). DOI:10.1016/j.cryobiol.2014.05.002 · 1.64 Impact Factor
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    ABSTRACT: Using a single tetradentate platinum emitter dubbed Pt7O7, efficient and stable white organic light-emitting diodes are developed. The excimer-based white devices achieve an external quantum efficiency (EQE) of 24.5%, coordinates of (0.37, 0.42) based on the Commission internationale de l'éclairage (CIE) system, and a color rendering index (CRI) of 70. Moreover, devices of Pt7O7 in a stable structure demonstrate operational lifetimes (50% initial luminance) of 36 h at an elevated driving current of 20 mA/cm(2) , which corresponds to over 10 000 h at 100 cd/m(2) .
    Advanced Materials 05/2014; 26(18). DOI:10.1002/adma.201305507 · 15.41 Impact Factor
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    ABSTRACT: The competence for embryonic development after IVF is low in the yak, therefore, we investigated the effects of supplementation of FSH, LH and the proteasome inhibitor MG132 in IVM media on yak oocyte competence for development after IVF. In Experiment 1, yak cumulus-oocyte complexes (COCs) were in vitro matured (IVM) in TCM-199 with 20% fetal calf serum (FCS), 1 microg/mL estradiol-17beta, and different combinations of LH (50 or 100 IU/mL) and FSH (0, 1, 5, 10 microg/mL) at 38.6 degrees C, 5% CO2 in air for 24 h. Matured oocytes were exposed to frozen-thawed, heparin-capacitated yak sperm. Presumptive zygotes were cultured in SOF medium containing 6 mg/ml BSA, 0.5 mg/mL myoinositol, 3% (v/v) essential amino acids, 1% nonessential amino acids and 100 mug/mL L-glutamine (48 h, 38.5 degrees C, 5% CO2, 5% O2, and 90% N2). In Experiment 2, cumulus cells were collected at the end of IVM to determine FSHR and LHR mRNA expression by real-time PCR. In Experiment 3 and 4, COCs were cultured in the presence or absence of the proteasomal inhibitor MG132 from either 0-6 h or 18-24 h after initiation of maturation. The optimum concentration of FSH and LH in IVM media was 5 microg/mL FSH and 50 IU/mL LH which resulted in the greatest cleavage (79.1%) and blastocyst rates (16.1%). Both FSHR and LHR mRNA were detected in yak cumulus cells after IVM. Treatment with MG132 early in maturation reduced (P < 0.05) cleavage and blastocyst rates. Conversely, treatment with MG132 late in maturation improved (P < 0.05) blastocyst rate. Optimal results with MG132 were achieved at a concentration of 10 microM. An optimum concentration of FSH and LH in IVM medium, and treatment with MG132 late in maturation can improve yak oocytes competence for development after IVF.
    Reproductive Biology and Endocrinology 04/2014; 12(1):30. DOI:10.1186/1477-7827-12-30 · 2.41 Impact Factor

Publication Stats

467 Citations
231.96 Total Impact Points

Institutions

  • 2012–2015
    • Southwest University for Nationalities
      Hua-yang, Sichuan, China
  • 2006–2015
    • Arizona State University
      • Department of Materials Science and Engineering
      Phoenix, Arizona, United States
  • 2004–2014
    • Beijing University of Posts and Telecommunications
      • State Key Laboratory of Information Photonics and Optical Communications
      Peping, Beijing, China
  • 2008–2012
    • Lanzhou Veterinary Research Institute
      Kao-lan-hsien, Gansu Sheng, China