Jia Li

Beijing University of Aeronautics and Astronautics (Beihang University), Peping, Beijing, China

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Publications (283)841.46 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Multi-camera pedestrian detection is the challenging problem in the field of surveillance video analysis. However, existing approaches may produce “phantoms” (i.e., fake pedestrians) due to the heavy occlusions in real surveillance scenario, while calibration errors and the diverse heights of pedestrians may also heavily decrease the detection performance. To address these problems, this paper proposes a robust multiple cameras pedestrian detection approach with multi-view Bayesian network model (MvBN). Given the preliminary results obtained by any multi-view pedestrian detection method, which are actually comprised of both real pedestrians and phantoms, the MvBN is used to model both the occlusion relationship and the homography correspondence between them in all camera views. As such, the removal of phantoms can be formulated as an MvBN inference problem. Moreover, to reduce the influence of the calibration errors and keep robust to the diverse heights of pedestrians, a height-adaptive projection (HAP) method is proposed to further improve the detection performance by utilizing a local search process in a small neighborhood of heights and locations of the detected pedestrians. Experimental results on four public benchmarks show that our method outperforms several state-of-the-art algorithms remarkably and demonstrates high robustness in different surveillance scenes.
    Pattern Recognition 05/2015; 48(5). DOI:10.1016/j.patcog.2014.12.004
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    ABSTRACT: Visual saliency is an effective tool for perceptual image processing. In the past decades, many saliency models have been proposed by primarily considering visual cues such as local contrast and global rarity. However, such explicit cues derived only from input stimuli are often insufficient to separate targets from distractors, leading to noisy saliency maps. In fact, the latent cues, especially the latent signal correlations that link visually distinct stimuli (e.g., various parts of a salient target), may also play an important role in saliency estimation. In this paper, we propose a graph-based approach for image saliency estimation by incorporating both explicit visual cues and latent signal correlations. In our approach, the latent correlations between various image patches are first derived according to the statistical prior obtained from 10 million reference images. After that, the informativeness of image patches and their latent correlations are jointly considered to construct a directed graph, on which a random walking process is performed to generate saliency maps that pop-out only the most salient locations. Experimental results show that our approach achieves impressive performances on three public image benchmarks.
    Signal Processing Image Communication 05/2015; DOI:10.1016/j.image.2015.05.005
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    ABSTRACT: AMP-activated protein kinase (AMPK), a conserved heterotrimeric kinase, serves as an energy sensor maintaining energy balance at both cellular and whole-body levels and plays multiple beneficial roles in carbohydrate and lipid metabolism, which makes AMPK an attractive target for diabetes and other metabolic disorders. To date, establishment of the physiologically relevant biochemical assay for AMPK has not been reported. Here we developed a phosphorylated AMPK protection assay based on a time-resolved fluorescence resonance energy transfer (TR-FRET) assay, using the protein phosphatase 2A (PP2A) to dephosphorylate AMPK. The partially dephosphorylated AMPK by PP2A had lower activity than phosphorylated AMPK. This specific TR-FRET assay for AMPK was optimized in the 384-well format and produced similar EC50 values for AMPK activators AMP and A769662 and a similar IC50 value for AMPK inhibitor compound C, as previously reported. Under the optimized conditions, the assay Z' factor calculated over 160 data points has an optimal value greater than 0.5, which is suitable for high-throughput screening. In conclusion, this phosphorylated AMPK protection assay we developed is very robust, sensitive, and simple to perform and may be useful as a high-throughput assay for identifying AMPK activators with the ability of preventing activated AMPK against dephosphorylation by phosphatase in the physiological conditions. © 2015 Society for Laboratory Automation and Screening.
    Journal of Biomolecular Screening 05/2015; DOI:10.1177/1087057115585471
  • PLoS ONE 05/2015; 10(5):e0125802. DOI:10.1371/journal.pone.0125802
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    ABSTRACT: A series of tetranuclear and hexanuclear gold(I) complexes with bridging diethyldithiocarbamate (Et2dtc) and diphosphine/triphosphine were synthesized, including [{Au2(µ-dpb)(µ-Et2dtc)}SbF6]n (1, dpb = 1,4-bis(diphenylphosphino)benzene), [Au2(µ-dpb)(Et2dtc)2] (2), [Au4(µ-dpn)(µ-Et2dtc)3](SbF6) (3, dpn = 1,4-bis(diphenylphosphino)naphthalene), [Au4(µ-dpn)(µ-Et2dtc)3][Au(Et2dtc)2](SbF6)2 (4), [Au4(µ-dpa)(Et2dtc)3](SbF6) (5, dpa = 9,10-bis(diphenylphosphino)anthracene) and [{Au6(dpep)2(Et2dtc)3}{(SbF6)3}]n (6, dpep = bis(2-diphenylphosphinoethyl)phenylphosphine). The structures of 3•CH2Cl2, 4•2CH2Cl2, 5•2CH2Cl2•H2O and 6 were characterized by X-ray crystallography. The intramolecular Au•••Au distances across bridging Et2dtc are 2.9376(7)2.9725(8) Å in 3•CH2Cl2, 2.9762(16)3.1078(14) Å in 4•2CH2Cl2, 2.9288(9)3.0514(10) Å in 5•2CH2Cl2•H2O, and 2.9236(12)3.1914(17) Å in 6, implying significant aurophilic interactions. These gold(I) complexes exhibit intense photoluminescence properties in solid state. Upon lowering the temperature from 298 to 77 K, complexes 5 and 6 exhibit obvious red-shift from 536 nm to 589 nm for 5 and from 538 nm to 564 nm for 6, implying significant luminescence thermochromism due to thermal contraction upon cooling.
    RSC Advances 04/2015; 5(44). DOI:10.1039/C5RA01831E
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    ABSTRACT: Two DNA damage-inducible genes in Saccharomyces cerevisiae, DDI2 and DDI3, are identical and encode putative HD domain-containing proteins, whose functions are currently unknown. Since Ddi2/3 also shows limited homology to a fungal cyanamide hydratase that converts cyanamide to urea, we tested the enzymatic activity of recombinant Ddi2. To this end, we developed a novel enzymatic assay and determined that the KM of the recombinant Ddi2/3 for cyanamide is 17.3 +/- 0.05 mM and its activity requires conserved residues in the HD domain. Unlike most other DNA damage-inducible genes, DDI2/3 is only induced by a specific set of alkylating agents and surprisingly is strongly induced by cyanamide. To characterize the biological function of DDI2/3, we sequentially deleted both DDI genes and found that the double mutant was unable to metabolize cyanamide and became much more sensitive to growth inhibition by cyanamide, suggesting that the DDI2/3 genes protect host cells from cyanamide toxicity. Despite the physiological relevance of the cyanamide induction, DDI2/3 is not involved in its own transcriptional regulation. The significance of cyanamide hydratase activity and its induced expression is discussed. Copyright © 2015, The American Society for Biochemistry and Molecular Biology.
    Journal of Biological Chemistry 04/2015; 290(20). DOI:10.1074/jbc.M115.645408
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    ABSTRACT: To investigate the effects of physalin B insolated from Physalis divericata on human colon cancer cells in vitro and its anticancer mechanisms. Human HCT116 colon cancer cell line was tested. Cell viability and apoptosis were detected, and relevant proteins were measured using Western blot analyses. Autophagosomes were observed in stable GFP-LC3 HCT116 cells. Localization of autophagosomes and lysosomes was evaluated in GFP-LC3/RFP-LAMP1-co-transfected cells. Microtubules and F-actin microfilaments were observed with confocal microscope. Mitochondrial ROS (mito-ROS) was detected with flow cytometry in the cells stained with MitoSox dye. Physalin B inhibited the viability of HCT116 cells with an IC50 value of 1.35 μmol/L. Treatment of the cells with physalin B (2.5-10 μmol/L) induced apoptosis and the cleavage of PARP and caspase-3. Meanwhile, physalin B treatment induced autophagosome formation, and accumulation of LC3-II and p62, but decreased Beclin 1 protein level. Marked changes of microtubules and F-actin microfilaments were observed in physalin B-treated cells, which led to the blockage of co-localization of autophagosomes and lysosomes. Physalin B treatment dose-dependently increased the phosphorylation of p38, ERK and JNK in the cells, whereas the p38 inhibitor SB202190, ERK inhibitor U0126 or JNK inhibitor SP600125 could partially reduce physalin B-induced PARP cleavage and p62 accumulation. Moreover, physalin B treatment dose-dependently increased mito-ROS production in the cells, whereas the ROS scavenger NAC could reverse physalin B-induced effects, including incomplete autophagic response, accumulation of ubiquitinated proteins, changes of microtubules and F-actin, activation of p38, ERK and JNK, as well as cell death and apoptosis. Physalin B induces mito-ROS, which not only inhibits the ubiquitin-proteasome pathway but also induces incomplete autophagic response in HCT116 cells in vitro.
    Acta Pharmacologica Sinica 04/2015; 36(4):517-27. DOI:10.1038/aps.2014.157
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    ABSTRACT: Sterol-regulatory element binding proteins (SREBPs) are major transcription factors that regulate liver lipid biosynthesis. In this article we reported a novel synthetic compound 2-(3-benzoylthioureido)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (ZJ001) that inhibited the SREBP-1c pathway, and effectively reduced hepatic lipid accumulation in diet-induced obesity (DIO) mice. A luciferase reporter driven by an SRE-containing promoter transfected into HepG2 cells was used to discover the compound. Two approaches were used to evaluate the lipid-lowering effects of ZJ001: (1) diet-induced obesity (DIO) mice that were treated with ZJ001 (15 mg·kg(-1)·d(-1), po) for 7 weeks; and (2) HepG2 cells and primary hepatocytes used as in vitro models. ZJ001 (10, 20 μmol/L) dose-dependently inhibited the activity of SRE-containing promoter. ZJ001 administration ameliorated lipid metabolism and improved glucose tolerance in DIO mice, accompanied by significantly reduced mRNA levels of SREBP-1C and SREBP-2, and their downstream genes. In HepG2 cells and insulin-treated hepatocytes, ZJ001 (10-40 μmol/L) dose-dependently inhibited lipid synthesis, and reduced mRNA levels of SREBP-1C and SREBP-2, and their downstream genes. Furthermore, ZJ001 dose-dependently increased the phosphorylation of AMPK and regulatory-associated protein of mTOR (Raptor), and suppressed the phosphorylation of mTOR in insulin-treated hepatocytes. Moreover, ZJ001 increased the ADP/ATP ratio in insulin-treated hepatocytes. ZJ001 exerts multiple beneficial effects in diet-induced obesity mice. Its lipid-lowering effects may result from the suppression of mTORC1, which regulates SREBP-1c transcription. The results suggest that the SREBP-1c pathway may be a potential therapeutic target for the treatment of lipid metabolic disorders.
    Acta Pharmacologica Sinica 04/2015; 36(4):483-96. DOI:10.1038/aps.2014.149
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    ABSTRACT: A new brominated polyunsaturated lipid, methyl (E,E)-14,14-dibromo-4,6,13-tetradecatrienoate (1), along with three known related analogues (2-4), were isolated from the Et2O-soluble portion of the acetone extract of Chinese marine sponge Xestospongia testudinaria treated with diazomethane. The structure of the new compound was elucidated by detailed spectroscopic analysis and by comparison with literature data. Compound 3 exhibited significant inhibitory activity against protein tyrosine phosphatase 1B (PTP1B), a key target for the treatment of type II diabetes and obesity, with an IC50 value of 5.30 ± 0.61 μM, when compared to the positive control oleanolic acid (IC50 = 2.39 ± 0.26 μM).
    Journal of Asian natural products research 04/2015; DOI:10.1080/10286020.2015.1026334
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    ABSTRACT: Cisplatin is a potent anti-cancer agent for various types of tumors. However, the clinical use of cisplatin is often limited by its nephrotoxicity. This study reports that WZ tablet (WZ, a preparation of an ethanol extract of Schisandra sphenanthera) mitigates cisplatin-induced toxicity in renal epithelial HK-2 cells and in mice. Pretreatment of HK-2 cells with WZ ameliorated cisplatin-induced cytotoxicity caused by oxidative stress, as was demonstrated by reductions in the levels of reactive oxygen species (ROS) and increased levels of glutathione (GSH). WZ facilitated the nuclear accumulation of the transcription factor NF-E2-related factor 2 (Nrf2) and the subsequent expression of its target genes such as quinine oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1) and glutamate cysteine ligase (GCL). Protective effects of WZ on cisplatin-induced nephrotoxicity were also observed in mice. WZ attenuated cisplatin-induced renal dysfunction, structural damage and oxidative stress. The nuclear accumulation of Nrf2 and its target genes were increased by WZ treatment. Taken together, these findings demonstrated WZ have a protective effect against cisplatin-induced nephrotoxicity by activation of the Nrf2 mediated defense response, which is of significant importance for therapeutic intervention in cisplatin induced renal injury. Copyright © 2015 Elsevier GmbH. All rights reserved.
    Phytomedicine 03/2015; 31(5). DOI:10.1016/j.phymed.2015.03.003
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    ABSTRACT: Identifying chemotherapy candidates with high selectivity against cancer cells is a major challenge in cancer treatment. Tumor microenvironments cause chronic endoplasmic reticulum (ER) stress and activate the unfolded protein response (UPR) as an adaptive response. Here, one novel small-molecule compound, 17#, was discovered as a potent pan-UPR inhibitor. It exhibited good selection for growth inhibition when cancer cells were cultured in 2-deoxy-D-glucose (2DG), mimicking an in vitro glucose-deprived status. Additionally, 17# alone could mildly suppress the growth of HeLa tumor xenografts, and a synergistic anti-cancer effect was observed when 17# was combined with 2DG. A mechanistic study showed that 17#-induced selective anti-cancer effects were highly dependent on UPR inhibition, and overexpressing GRP78 or XBP1s reversed the 17#-induced growth inhibition and cell cycle arrest, partially by delaying the downregulation of the cell cycle regulator cyclin B1. Furthermore, 17# improved the sensitivity of anti-cancer drugs such as doxorubicin or etoposide. Our study presents evidence that disrupting the UPR has selective therapeutic potential and may enhance drug sensitivity. Copyright © 2015. Published by Elsevier Ireland Ltd.
    Cancer Letters 02/2015; 360(2). DOI:10.1016/j.canlet.2015.02.029
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    ABSTRACT: Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct that can be firmly immobilized on a graphene-spotted screen printed electrode via strong π-interactions. The inherent current signal produced by the surface-confined glycoquinone can be used to detect selective sugar–protein recognitions with simple electrochemical techniques and portable facilities. Importantly, we demonstrate that the level of pathogenic receptors expressed by different types of live cells can be tracked with the electrode system in a label-free manner, providing a useful tool for the on-demand disease diagnosis as well as basic biochemical studies.
    Chemical Science 02/2015; 6(3). DOI:10.1039/C4SC03614J
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    ABSTRACT: A series of novel 3-(furo[2,3-b]pyridin-3-yl)-4-(1H-indol-3-yl)-maleimides were designed, synthesized and biologically evaluated for their GSK-3β inhibitory activities. Most compounds showed favorable inhibitory activities against GSK-3β protein. Among them, compounds 5n, 5o and 5p significantly reduced GSK-3β substrate Tau phosphorylation at Ser396 in primary neurons, indicating inhibition of cellular GSK-3β activity. In the in vitro neuronal injury models, compounds 5n, 5o and 5p prevented neuronal death against glutamate, oxygen-glucose deprivation and nutrient serum deprivation which are closely associated with cerebral ischemic stroke. In the in vivo cerebral ischemia animal model, compound 5o reduced infarct size by 10% and improved the neurological deficit. The results may provide new insights into the development of novel GSK-3β inhibitors with potential neuroprotective activity against brain ischemic stroke. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Chemical Biology &amp Drug Design 02/2015; DOI:10.1111/cbdd.12546
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    ABSTRACT: Six new guignardins A-F (1-6) were isolated from the cultures of endophytic fungus Guignardia sp. KcF8 derived of a mangrove plant Kandelia candel, along with three known analogues, palmarumycins C-1 (7), BG1 (8), and JC1 (9). Compounds 2, 3, 7, and 8 showed antimicrobial activities. Compounds 5-7 exhibited significant cytotoxicities against 10 human tumor cell lines. Compound 3 also displayed significant inhibitory activity against human protein tyrosine phosphatase 1B and histone deacetylase silent information regulator T-1 enzymes, two key targets for the treatment of diabetes. This is the first report on the anti-PTP1B and anti-SIRT1 activities of spirodioxynaphthalenes.
    ChemInform 02/2015; 46(5). DOI:10.1002/chin.201505211
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    ABSTRACT: We have developed a galactosyl azidonaphthalimide probe for the selective fluorogenic imaging of hepatocellular H2S an important gaseous transmitter produced in the liver.
    Chemical Communications 01/2015; 51(17). DOI:10.1039/C4CC09771H
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    ABSTRACT: Galiellalactone analogs (1-4) (including two new compounds), together with their possible precursors (5-9, named pregaliellalactone B-F), were obtained from the solid cultures of an endophytic fungus Sarcosomataceae NO.45-1-8-1. Their chemical structures were elucidated by analyses of HR ESI-TOF MS, 1D-, 2D-NMR, CD spectra and single crystal X-ray diffraction methods. Compounds 5-9, the possible precursors of galiellalactone analogs, were found to exist as enantiomers for the first time. The cytotoxicity of these compounds against six tumor cell lines was examined and preliminary structure-activity relationship (SAR) was also discussed. Copyright © 2015. Published by Elsevier B.V.
    Fitoterapia 01/2015; 101. DOI:10.1016/j.fitote.2015.01.002
  • Chinese Journal of Organic Chemistry 01/2015; 35(1):191. DOI:10.6023/cjoc201405032
  • IEEE Transactions on Pattern Analysis and Machine Intelligence 01/2015; DOI:10.1109/TPAMI.2015.2424870
  • Chinese Journal of Organic Chemistry 01/2015; 35(1):129. DOI:10.6023/cjoc201405020
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    ABSTRACT: Using Illumina sequencing technology, we have generated the large-scale transcriptome sequencing data and indentified many putative genes involved in isoflavones biosynthesis in Pueraria lobata. Pueraria lobata, a member of the Leguminosae family, is a traditional Chinese herb which has been used since ancient times. P. lobata root has extensive clinical usages, because it contains a rich source of isoflavones, including daidzin and puerarin. However, the knowledge of isoflavone metabolism and the characterization of corresponding genes in such a pathway remain largely unknown. In this study, de novo transcriptome of P. lobata root and leaf was sequenced using the Solexa sequencing platform. Over 140 million high-quality reads were assembled into 163,625 unigenes, of which about 43.1 % were aligned to the Nr protein database. Using the RPKM (reads per kilo bases per million reads) method, 3,148 unigenes were found to be upregulated, and 2,011 genes were downregulated in the leaf as compared to those in the root. Towards a further understanding of these differentially expressed genes, Gene ontology enrichment and metabolic pathway enrichment analyses were performed. Based on these results, 47 novel structural genes were identified in the biosynthesis of isoflavones. Also, 22 putative UDP glycosyltransferases and 45 O-methyltransferases unigenes were identified as the candidates most likely to be involved in the tailoring processes of isoflavonoid downstream pathway. Moreover, MYB transcription factors were analyzed, and 133 of them were found to have higher expression levels in the roots than in the leaves. In conclusion, the de novo transcriptome investigation of these unique transcripts provided an invaluable resource for the global discovery of functional genes related to isoflavones biosynthesis in P. lobata.
    Plant Cell Reports 12/2014; DOI:10.1007/s00299-014-1733-1

Publication Stats

3k Citations
841.46 Total Impact Points

Institutions

  • 2015
    • Beijing University of Aeronautics and Astronautics (Beihang University)
      • State Key Laboratory for Virtual Reality Technology and Systems
      Peping, Beijing, China
  • 2010–2015
    • University of Saskatchewan
      • Department of Microbiology and Immunology
      Saskatoon, Saskatchewan, Canada
    • Shanghai Institute of Microsystem And Information Technology
      Shanghai, Shanghai Shi, China
    • University of Jinan (Jinan, China)
      Chi-nan-shih, Shandong Sheng, China
  • 2005–2015
    • Chinese Academy of Sciences
      • • State Key Laboratory of Drug Research
      • • National Center for Drug Screening
      • • State Key Laboratory of Structural Chemistry
      Peping, Beijing, China
  • 2014
    • Renji Hospital
      Shanghai, Shanghai Shi, China
    • China Pharmaceutical University
      Nan-ching-hsü, Jiangxi Sheng, China
  • 2013–2014
    • Peking University
      • School of Electronics Engineering and Computer Science
      Peping, Beijing, China
    • Jilin University
      • State Key Lab of Superhard Materials
      Yung-chi, Jilin Sheng, China
    • Changsha Medical University
      Ch’ang-sha-shih, Hunan, China
    • Zhejiang University of Technology
      Hang-hsien, Zhejiang Sheng, China
  • 2011–2014
    • East China Normal University
      • • Department of Chemistry
      • • School of Life Sciences
      Shanghai, Shanghai Shi, China
    • Sun Yat-Sen University
      • • Department of Chemical Engineering
      • • School of Pharmaceutical Science
      Shengcheng, Guangdong, China
    • University of South China
      Heng-nan, Hunan, China
    • Ocean University of China
      Tsingtao, Shandong Sheng, China
    • Nanyang Technological University
      • School of Computer Engineering
      Tumasik, Singapore
  • 2009–2014
    • Shanghai Research Institute of Materials
      Shanghai, Shanghai Shi, China
    • Zhejiang University
      • College of Pharmaceutical Sciences
      Hang-hsien, Zhejiang Sheng, China
    • Henan Provincial People’s Hospital
      Cheng, Henan Sheng, China
  • 2005–2014
    • Sichuan University
      • • State Key Laboratory of Oral Diseases
      • • Department of Oral and Maxillofacial Surgery
      Hua-yang, Sichuan, China
  • 2003–2014
    • Shanghai Institutes for Biological Sciences
      • Institute of Health Sciences
      Shanghai, Shanghai Shi, China
  • 2009–2013
    • Shanghai Jiao Tong University
      • Department of Chemical Engineering (CHE)
      Shanghai, Shanghai Shi, China
  • 2008–2013
    • National Shanghai Center for New Drug Evaluation and Research
      Shanghai, Shanghai Shi, China
    • Wuhan University
      Wu-han-shih, Hubei, China
  • 2012
    • Chinese Center For Disease Control And Prevention
      • Institute for Viral Disease Control and Prevention
      Peping, Beijing, China
    • University of Texas MD Anderson Cancer Center
      • Department of Molecular Carcinogenesis
      Houston, TX, United States
  • 2006
    • North China Institute of Computing Technology
      Peping, Beijing, China
  • 2003–2004
    • University of Kansas
      Lawrence, Kansas, United States