Jie Li

Fudan University, Shanghai, Shanghai Shi, China

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Publications (44)114.22 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Angiogenesis inducing agents, such as Sildenafil, can be used to treat stroke. In this study, USPIO-enhanced MRI was used to visualize collateral vessels in the penumbral area of lesions of rats subjected to transient middle cerebral artery occlusion (tMCAO), and the evaluate efficacy of the Sildenafil treatment. Here, P904, a USPIO agent, was used as a T1 reducing agent at a low dose for MR angiography and as a T2* reducing agent at a moderate dose for visualization of very small vessels on gradient echo susceptibility-weighted imaging (SWI). SWI scans without/with USPIO injection were performed for the following cases: tMCAO without any treatment (n=5), tMCAO with Sildenafil treatment (n=6), and sham tMCAO (n=1) at four different time points (i.e., baseline, 24 h, two weeks and four weeks after tMCAO). The presence of newly developed vessels in the periphery of the ischemic core was only observed in those animals showing severe ischemic brain damage and at the same time received Sildenafil treatment at two weeks post tMCAO. This work suggests that, at 7 T, high resolution SWI in conjunction with USPIO injection at a moderate dose can be used to visualize post-stroke angiogenesis in the tMCAO rat model. Full-text article is available from http://magres.wipm.ac.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=9623
    Chinese Journal of Magnetic Resonance. 03/2014; 31(1):20-31.
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    ABSTRACT: Aurora kinases play a key role in the regulation of mitosis and have been regarded as promising targets of cancer therapy. In this paper we describe a thienopyrimidine derivative (S7), a novel potent ATP-competitive hit inhibitor of Aurora B kinase screened through a HTS system, with the IC50 141.12 nM in the biochemical kinase activity assay. Human tumor cells treated with S7 showed dose-dependent inhibition of auto-phosphorylation of Aurora B on Thr232 and another widely-used marker specific for Aurora B kinase, the phosphorylation of Histone H3 (Ser 10), demonstrating endogenous Aurora B kinase activity were inhibited at cellular level. Moreover, S7 treatment induced proliferation inhibition, colony formation inhibition and apoptosis of human tumor cell lines in a dose- and time-dependent manner.
    European journal of medicinal chemistry 05/2013; 65C:151-157. · 3.27 Impact Factor
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    ABSTRACT: Aurora kinases, frequently detected to be over-expressing in human tumors, regulate many essential events during mitosis progression and have been regarded as potentially important targets for cancer therapy. S39 is a novel potent inhibitor of Aurora B kinase with the IC(50) 90.07 nM in the biochemical assay in an ATP competitive manner. S39 treatment on human tumor cells can inhibit the phosphorylation of Histone H3 (Ser10), a direct downstream substrate of Aurora B kinase, indicating S39 inhibits endogenous Aurora B kinase activity in cell-based level. Furthermore, S39 treatment blocks cell proliferation, inhibits colony formation and induces apoptosis in a wide range of human tumor cell lines. These results indicate that S39 is a potential lead compound to be an Aurora B inhibitor.
    Biotechnology Letters 02/2013; · 1.85 Impact Factor
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    ABSTRACT: BACKGROUND: Hydrocephalus is a central nervous system (CNS) disorder characterized by the abnormalaccumulation of cerebrospinal fluid (CSF) in cerebral ventricles, resulting their dilatation andassociated brain tissue injury. The pathogenesis of hydrocephalus remains unclear; however,recent reports suggest the possible involvement of abnormal osmotic gradients. Here weexplore the kinetics associated with manipulating CSF osmolarity on ventricle volume (VV)in the normal rat brain. METHODS: CSF was made hyper-osmotic by introducing 10KD dextran into the lateral ventricle, eitherby acute injection at different concentrations or by chronic infusion at a single concentration.The induction and withdrawal kinetics of dextran infusion on VV were explored in bothcontexts. RESULTS: Acute intraventricular injection of dextran caused a rapid increase in VV which completelyreversed within 24 hours. These kinetics are seemingly independent of the CSF osmolarity across a range spanning an order of magnitude; however, the magnitude of the transientincrease in VV was proportional to CSF osmolarity. By contrast, continuous intraventricularinfusion of dextran at a relatively low concentration caused a more gradual increase in VVwhich was very slow to reverse when infusion was suspended after five days. CONCLUSION: We conclude that hyperosmolar CSF is sufficient to produce a proportional degree ofhydrocephalus in the normal rat brain, and that this phenomenon exhibits hysteresis if CSFhyperosmolarity is persistent. Thus pathologically-induced increases in CSF osmolarity maybe similarly associated with certain forms of clinical hydrocephalus. An improvedunderstanding of this phenomenon and its kinetics may facilitate the development of noveltherapies for the treatment of clinical hydrocephalus.
    Fluids and barriers of the CNS. 07/2012; 9(1):13.
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    ABSTRACT: Brain edema following stroke is a critical clinical problem due to its association with increased morbidity and mortality. Despite its significance, present treatment for brain edema simply provides symptomatic relief due to the fact that molecular mechanisms underlying brain edema remain poorly understood. The present study investigated the role of hypoxia-inducible factor-1α (HIF-1α) and aquaporins (AQP-4 and -9) in regulating cerebral glycerol accumulation and inducing brain edema in a rodent model of stroke. Two-hours of middle cerebral artery occlusion (MCAO) followed by reperfusion was performed in male Sprague-Dawley rats (250-280 g). Anti-AQP-4 antibody, anti-AQP-9 antibody, or 2-Methoxyestradiol (2ME2, an inhibitor of HIF-1α) was given at the time of MCAO. The rats were sacrificed at 1 and 24 hours after reperfusion and their brains were examined. Extracellular and intracellular glycerol concentration of brain tissue was calculated with an enzymatic glycerol assay. The protein expressions of HIF-1α, AQP-4 and AQP-9 were determined by Western blotting. Brain edema was measured by brain water content. Compared to control, edema (p < 0.01), increased glycerol (p < 0.05), and enhanced expressions of HIF-1α, AQP-4, and AQP-9 (p < 0.05) were observed after stroke. With inhibition of AQP-4, AQP-9 or HIF-1α, edema and extracellular glycerol were significantly (p < 0.01) decreased while intracellular glycerol was increased (p < 0.01) 1 hour after stroke. Inhibition of HIF-1α with 2ME2 suppressed (p < 0.01) the expression of AQP-4 and AQP-9. These findings suggest that HIF-1α serves as an upstream regulator of cerebral glycerol concentrations and brain edema via a molecular pathway involving AQP-4 and AQP-9. Pharmacological blockade of this pathway in stroke patients may provide novel therapeutic strategies.
    Current neurovascular research 02/2011; 8(1):44-51. · 3.23 Impact Factor
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    ABSTRACT: In this paper, the minimal visual coverage shortest path in raster terrain is studied with the proposal of a hypervolume contribution based multiobjective evolutionary approach. The main feature of the presented method is that all individuals in the population are periodically replaced by the selected non-dominated candidates in the archive based on hypervolume contribution, besides the well designed evolution- ary operators and some popular techniques such as dominated relation and archive. Our algorithm may obtain well distributed Pareto set approximation efficiently, which is superior to the implementations based on the framework of NSGA-II and SMS- EMOA with respect to the hypervolume.
    Proceedings of the IEEE Congress on Evolutionary Computation, CEC 2011, New Orleans, LA, USA, 5-8 June, 2011; 01/2011
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    ABSTRACT: There is a wide range of applications in both military and civil fields for optimal path problem in raster terrain whose cost is visibility information. For the path search problem whose objective is maximal average horizon, the traditional algorithms are unsuitable due to the characteristic of the problem. This paper presents a method based on evolutionary algorithm, which may rapidly get the optimal solution by designing multiple effective evolutionary operators and self-adaptively adjusting the probability of each mutator. Experiments show that the method presented is superior to the simulated annealing algorithm.
    Computer Science and Information Technology (ICCSIT), 2010 3rd IEEE International Conference on; 08/2010
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    ABSTRACT: Human carbonyl reductase 1 (CBR1) converts the antitumor drug and anthracycline daunorubicin (DNR) into the alcohol metabolite daunorubicinol (DNROL) with significantly reduced antitumor activity and cardiotoxicity, and this limits the clinical use of DNR. Inhibition of CBR1 can thus increase the efficacy and decrease the toxicity of DNR. Here we report that (-)-epigallocatechin gallate (EGCG) from green tea is a promising inhibitor of CBR1. EGCG directly interacts with CBR1 and acts as a noncompetitive inhibitor with respect to the cofactor reduced nicotinamide adenine dinucleotide phosphate and the substrate isatin. The inhibition is dependent on the pH, and the gallate moiety of EGCG is required for activity. Molecular modeling has revealed that EGCG occupies the active site of CBR1. Furthermore, EGCG specifically enhanced the antitumor activity of DNR against hepatocellular carcinoma SMMC7721 cells expressing high levels of CBR1 and corresponding xenografts. We also demonstrated that EGCG could overcome the resistance to DNR by Hep3B cells stably expressing CBR1 but not by RNA interference of CBR1-HepG2 cells. The level of the metabolite DNROL was negatively correlated with that of EGCG in the cell extracts. Finally, EGCG decreased the cardiotoxicity of DNR in a human carcinoma xenograft model with both SMMC7721 and Hep3B cells in mice. Conclusion: These results strongly suggest that EGCG can inhibit CBR1 activity and enhance the effectiveness and decrease the cardiotoxicity of the anticancer drug DNR. These findings also indicate that a combination of EGCG and DNR might represent a novel approach for hepatocellular carcinoma therapy or chemoprevention.
    Hepatology 08/2010; 52(2):703-14. · 12.00 Impact Factor
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    ABSTRACT: We recently reported a lymphatic cerebrospinal fluid (CSF) absorption deficit in a kaolin model of communicating hydrocephalus in rats with ventricular expansion correlating negatively with the magnitude of the impediment to lymphatic function. However, it is possible that CSF drainage was not significantly altered if absorption at other sites compensated for the lymphatic defect. The purpose of this study was to investigate the impact of the lymphatic absorption deficit on global CSF absorption (CSF outflow resistance). Kaolin was injected into the basal cisterns of Sprague Dawley rats. The development of hydrocephalus was assessed using magnetic resonance imaging (MRI). In one group of animals at about 3 weeks after injection, the movement of intraventricularly injected iodinated human serum albumin (125I-HSA) into the olfactory turbinates provided an estimate of CSF transport through the cribriform plate into nasal lymphatics (n = 18). Control animals received saline in place of kaolin (n = 10). In a second group at about 3.5 weeks after kaolin injection, intraventricular pressure was measured continuously during infusion of saline into the spinal subarachnoid space at various flow rates (n = 9). CSF outflow resistance was calculated as the slope of the steady-state pressure versus flow rate. Control animals for this group either received no injections (intact: n = 11) or received saline in place of kaolin (n = 8). Compared to saline injected controls, lateral ventricular volume in the kaolin group was significantly greater (0.087 +/- 0.013 ml, n = 27 versus 0.015 +/- 0.001 ml, n = 17) and lymphatic function was significantly less (2.14 +/- 0.72% injected/g, n = 18 versus 6.38 +/- 0.60% injected/g, n = 10). Additionally, the CSF outflow resistance was significantly greater in the kaolin group (0.46 +/- 0.04 cm H2O microL(-1) min, n = 9) than in saline injected (0.28 +/- 0.03 cm H2O microL(-1) min, n = 8) or intact animals (0.18 +/- 0.03 cm H2O microL(-1) min, n = 11). There was a significant positive correlation between CSF outflow resistance and ventricular volume. The data suggest that the impediment to lymphatic CSF absorption in a kaolin-induced model of communicating hydrocephalus has a significant impact on global CSF absorption. A lymphatic CSF absorption deficit would appear to play some role (either direct or indirect) in the pathogenesis of ventriculomegaly.
    Cerebrospinal Fluid Research 02/2010; 7(1):4. · 1.81 Impact Factor
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    ABSTRACT: This paper addresses the complete visual coverage path planning problem in raster terrain and presents an effective solution with hierarchy based on multiple observers siting and an evolutionary path planning algorithm. The main idea of the method is to get a nearly optimal complete visual coverage path by connecting all of the observers in the visual coverage set. Firstly a new deterministic algorithm for a quasi minimal number set of observers is presented, which is superior to the general greedy algorithm in the time complexity and the quality of solutions. On the basis of that we further present a hierarchy path planning method whose core is an evolutionary algorithm. The advantage of the proposed method is that it realizes really complete visual coverage with a comparatively optimal path and easy to extend to the version of multiple paths.
    2012 Fifth International Joint Conference on Computational Sciences and Optimization. 01/2010; 1:497-500.
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    ABSTRACT: Popular circulation theory of hydrocephalus assumes that the brain is impermeable to cerebrospinal fluid (CSF), and is therefore incapable of absorbing the CSF accumulating within the ventricles. However, the brain parenchyma is permeable to water due to the presence of specific ion channels as well as aquaporin channels. Thus, the movement of water into and out of the ventricles may be determined by the osmotic load of the CSF. If osmotic load determines the aqueous content of CSF in this manner, it is reasonable to hypothesize that hydrocephalus may be precipitated by pathologies and/or insults that produce sustained elevations of osmotic content within the ventricles. We investigated this hypothesis by manipulating the osmotic content of CSF and assaying the development of hydrocephalus in the rat brain. This was achieved by continuously infusing artificial CSF (negative control; group I), fibroblast growth factor (FGF2) solution (positive control; group II) and hyperosmotic dextran solutions (10 KD and 40 KD as experimental solutions: groups III and IV) for 12 days at 0.5 muL/h. The osmolality of the fluid infused was 307, 664, 337 and 328 mOsm/L in Groups I, II, III and IV, respectively. Magnetic resonance imaging (MRI) was used to evaluate the ventricular volumes. Analysis of variance (ANOVA) with pairwise group comparisons was done to assess the differences in ventricular volumes among the four groups. Group I had no hydrocephalus. Group II, group III and group IV animals exhibited significant enlargement of the ventricles (hydrocephalus) compared to group I. There was no statistically significant difference in the size of the ventricles between groups II, III and IV. None of the animals with hydrocephalus had obstruction of the aqueduct or other parts of CSF pathways on MRI. Infusing hyperosmolar solutions of dextran, or FGF into the ventricles chronically, resulted in ventricular enlargement. These solutions increase the osmotic load in the ventricles. Water influx (through the choroid plexus CSF secretion and/or through the brain) into the ventricles to normalize this osmotic gradient results in hydrocephalus. We need to revise the popular theory of how fluid accumulates in the ventricles at least in some forms of hydrocephalus.
    Cerebrospinal Fluid Research 12/2009; 6:16. · 1.81 Impact Factor
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    ABSTRACT: The Ly-6 protein superfamily is usually identified as a group of proteins with a LU protein domain. LU domain is about 80 amino acids long and characterized by a conserved pattern of 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain containing gene, LYPD6, isolated from human testis cDNA library, and mapped to 2q23.1-23.2 by searching the UCSC genomic database. The LYPD6 cDNA sequence of 3,501 base pairs contains an open reading frame encoding 171 amino acids. Subcellular localization of LYPD6 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that LYPD6 was widely expressed in human tissues and the expression levels in brain and heart were relatively high. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD6 in HEK 293T cells was able to suppress the transcriptional activities of AP1.
    Molecular Biology Reports 09/2009; 37(4):2055-62. · 2.51 Impact Factor
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    ABSTRACT: The Aurora kinases play a critical role in mitosis and have been suggested as promising targets for cancer therapy due to their frequent overexpression in a variety of tumors. Compared with established inhibitors of cell division such as the anti-tubulins, novel agents target mitotic enzymes and show similar efficacy but with fewer side effects. Several small-molecule inhibitors of Aurora kinases have been developed as anticancer agents, some of which have progressed to early clinical evaluation. Here we identified 3-hydroxyflavone as a novel Aurora B inhibitor through high throughput screening. 3-Hydroxyflavone showed potent inhibition to Aurora B with the IC(50) on a nanomolar basis in the enzyme-based kinase activity assay. In the cell-based western blotting analysis, 3-hydroxyflavone dramatically decreased the phosphorylation level of Histone H3 on the site of serine 10, demonstrating the potent endogenous Aurora B activity inhibition in cell level. The followed cell image analysis provided the consist result. To make it clear whether 3-hydroxyflavone inhibited Aurora B by direct binding or not, SPR analysis was carried out to measure the affinity of interaction between Aurora B protein and 3-hydroxyflavone and the result proved the binding with high affinity. Usually Aurora activity suppression induced cancer cell proliferation inhibition. Colony formation and cell viability with/without treatment of 3-hydroxyflavone were measured using CCK-8. The growth suppression under 3-hydroxyflavone present and the growth recovery after being released gave strong evidence that presence of 3-hydroxyflavone efficiently inhibited the fast growth of cancer cells.
    Molecular Biology Reports 06/2009; 37(3):1577-83. · 2.51 Impact Factor
  • Cerebrospinal Fluid Research 01/2009; 6:1-1. · 1.81 Impact Factor
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    Cerebrospinal Fluid Research 01/2009; 6 Suppl 2:S10. · 1.81 Impact Factor
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    ABSTRACT: Communicating hydrocephalus (CH) occurs frequently, but clinically-relevant animal models amenable to diagnostic imaging and cerebrospinal fluid shunting are not available. In order to develop and characterize models of subarachnoid space (SAS) obstruction at the basal cisterns (BC) or cerebral convexities (CX), 25% kaolin was injected in adult female Sprague-Dawley rats following halothane anesthesia; intact- or saline-injected animals served as controls. For BC animals (n=28 hydrocephalics, n=20 controls), an anterior approach to the C1-clivus interval was employed and 30 microl of kaolin or saline was injected. For CX injections (n=13 hydrocephalics, n=3 controls), 50-60 microl of kaolin was injected bilaterally after separating the partitions in the SAS. In BC-injected rats, kaolin was observed grossly in the basal cisterns but not in the cisterna magna or at the foramina of Luschka, indicating that communicating (or extra-ventricular)--not obstructive--hydrocephalus had been induced. Following ketamine/xylazine anesthesia, magnetic resonance imaging (MRI) of gadolinium injected into the lateral ventricle also demonstrated CSF flow from the foramina of Luschka. MRI also revealed that ventriculomegaly progressed steadily in BC animals and by 2 weeks post-kaolin the mean Evan's ratio (frontal horn) increased significantly (mean 0.45 compared to 0.31 in intact- and 0.34 in saline-injected controls; p<0.001 for each). CX animals exhibited kaolin deposits covering approximately 80% of the cerebral hemispheres and developed noticeable ventriculomegaly (mean Evan's ratio 0.40), which was significant relative to intact animals (p=0.011) but not saline-injected controls. Surprisingly, ventriculomegaly following CX injections was less severe and much more protracted, requiring 3-4 months to develop compared to ventriculomegaly produced by BC obstruction. No hydrocephalic animals demonstrated obvious neurological deficits, but BC-injected animals that subsequently developed more severe ventriculomegaly exhibited nasal discharges and "coughing" for several days following kaolin injection. The new BC model is relevant because the clinical presentation of CH in children is often associated with obstruction at this site, while the CX model may be more representative of late adult onset normal pressure hydrocephalus.
    Experimental Neurology 06/2008; 211(2):351-61. · 4.65 Impact Factor
  • Neurosurgery 01/2008; 62(6). · 2.53 Impact Factor
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    ABSTRACT: The in vivo biocompatibility of pure sapphire and borosilicate glass (BSG) implanted onto the cerebral cortex was studied via magnetic resonance imaging (MRI) and histopathology. Each implant was embedded onto the cortical surface of an adult rat brain for a total of 28 days. Rats underwent surgery with and without implants, and rats with purposely damaged cortical implant sites were also studied. Each animal was imaged via MRI before surgery as well as 10 and 28 days after the surgery. Histopathological results of animals were obtained on the 28th day to determine the specific effect on neurons. Despite the fact that sapphire has been widely used in a variety of medical implants, both MRI and histopathological results indicate that pure sapphire is not biocompatible with the cerebral cortex. On the contrary, BSG implants appear to be biocompatible with the cortical surface.
    Magnetic Resonance Imaging 12/2007; 25(9):1333-40. · 2.06 Impact Factor
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    ABSTRACT: Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate and alcohol. GDPD5 has been reported in Mus musculus and Gallus gallus, but not in Homo sapiens. Here we report the cloning and characterization of a novel human GDPD domain-containing gene, GDPD5, isolated from human testis cDNA library, and mapped to 11q13.4-13.5 by searching the UCSC genomic database. The GDPD5 cDNA sequence of 3442 base pairs contains an open reading frame encoding 605 amino acids. The GDPD5 gene consists of 17 exons and encodes a putative protein with six transmembrane regions and a GDPD motif. Subcellular localization of GDPD5 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that GDPD5 was widely expressed in human tissues and the expression levels in kidney and prostate were relatively low.
    Molecular Biology Reports 07/2007; 35(3):351-9. · 2.51 Impact Factor
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    ABSTRACT: A self-assembled monolayer (SAM) of fluoroalkyl silane (FAS) (CF(3)(CF(2))(5)(CH(2))(2)SiCl(3)) was deposited on the surface of silicon wafers, aiming to enhance its stability and to reduce the inflammatory response in the central nervous system. Following implantation of the FAS SAM coated silicon in rat brains, the FAS SAM coating failed to reduce the inflammatory response of silicon, because it could not prevent the corrosion of the underlying silicon. The corrosion was hindered for the initial 10 days by the FAS SAM coating, but there was nearly no difference when compared to the uncoated silicon when the implantation periods were extended to 28 and 90 days. The FAS SAM coating was completely removed within 28 and 90 days. Meanwhile, on all the extracted uncoated and FAS SAM coated silicon wafers, there were proteinaceous substances deposited on the surfaces, and the amount of the deposits increased with exposure time.
    Journal of Biomedical Materials Research Part A 06/2007; 81(2):363-72. · 2.83 Impact Factor

Publication Stats

483 Citations
126 Downloads
2k Views
114.22 Total Impact Points

Institutions

  • 2006–2013
    • Fudan University
      • • Institute of Genetics
      • • State Key Laboratory of Genetic Engineering
      Shanghai, Shanghai Shi, China
    • University of Texas Health Science Center at San Antonio
      • Department of Neurosurgery
      San Antonio, TX, United States
  • 2012
    • State University of New York Upstate Medical University
      • Department of Neurosurgery
      Syracuse, New York, United States
  • 2001–2011
    • Wayne State University
      • Department of Anatomy and Cell Biology
      Detroit, Michigan, United States
  • 2010
    • University of Electronic Science and Technology of China
      Hua-yang, Sichuan, China
    • University of Science and Technology of China
      • Department of Electronic Engineering and Information Science
      Hefei, Anhui Sheng, China
  • 2009
    • University of Utah
      • Primary Children's Medical Center
      Salt Lake City, UT, United States
  • 2008
    • Children's Hospital of Michigan
      Detroit, Michigan, United States