[Show abstract][Hide abstract] ABSTRACT: Platelet endothelial cell adhesion molecule (PECAM or CD31) is a cell adhesion molecule expressed on circulating leukocytes and endothelial cells that plays an important role in mediating neutrophil and monocyte transendothelial migration in vivo. In this study, we investigated whether eosinophils, like neutrophils and monocytes, utilize PECAM for tissue recruitment to sites of allergic inflammation in vivo. Eosinophils express similar levels of PECAM as neutrophils as assessed by FACS analysis. RT-PCR studies demonstrate that eosinophils like neutrophils express the six extracellular domains of PECAM. Eosinophils exhibit homophilic binding to recombinant PECAM as assessed in a single-cell micropipette adhesion assay able to measure the biophysical strength of adhesion of eosinophils to recombinant PECAM. The strength of eosinophil adhesion to recombinant PECAM is the same as that of neutrophil binding to recombinant PECAM and can be inhibited with an anti-PECAM Ab. Although eosinophils express functional PECAM, anti-PECAM Abs did not inhibit bronchoalveolar lavage eosinophilia, lung eosinophilia, and airway hyperreactivity to methacholine in a mouse model of OVA-induced asthma in vivo. Thus, in contrast to studies that have demonstrated that neutrophil and monocyte tissue recruitment is PECAM dependent, these studies demonstrate that eosinophil tissue recruitment in vivo in this model is PECAM independent.
The Journal of Immunology 09/2001; 167(4):2292-7. · 5.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Conclusion A variety of DNA-based methods of modulating the immune and/or inflammatory response in animal models of asthma have shown
significant promise. Antisense strategies seek to inhibit expression of specific host genes, whereas DNA vaccines aim to modulate
the immune response to specific encoded allergens. In contrast, ISS DNA therapy seeks to redirect the host immune response
from a Th2 to a Th1 response. Current human studies will help to determine which, if any, of these DNA based therapies is
a safe and effective therapy for human asthma.
Springer Seminars in Immunopathology 02/2000; 22(1-2):117-24. · 4.17 Impact Factor