Publications (2)3.82 Total impact
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Article: Prevalence of human papillomavirus in Chinese patients with colorectal cancer.
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ABSTRACT: The prevalence of human papillomavirus (HPV) was determined in Chinese patients with colorectal cancer (CRC). The study also aimed to determine whether the HPV DNA peripheral blood (PB) assay can be used to diagnose HPV-related CRC. Tumour tissue, noncancerous colorectal tissue and whole-blood samples were obtained from 96 patients with CRC. In addition, 32 colorectal tissue samples were harvested from patients without CRC, and 48 whole-blood samples were collected from healthy blood donors. HPV DNA was detected by means of a nested polymerase chain reaction (PCR) using consensus primers, and HPV genotypes were determined by reverse Southern blot and pyrosequencing. HPV DNA was detected in 32 of the 96 patients with CRC, and colorectal tissues from the 32 control patients without CRC were negative for HPV DNA (P < 0.001). Among 48 healthy donors, three had detectable levels of HPV DNA in their PB. Patients with CRC did not have significantly higher levels of HPV DNA than controls. The HPV prevalence in tumour tissues was higher than that in noncancerous colorectal tissues (P < 0.001) or that in PB samples (P < 0.001). No correlation between the presence of HPV and demographic or medical characteristics was observed. HPV 16 was the viral type most frequently detected and was found in 33 (94%) of 35 HPV-positive patients. HPV infection may be a risk factor for CRC. However, detection of HPV DNA in PB does not appear to reflect the HPV status of CRC.Colorectal Disease 08/2011; 13(8):865-71. · 2.93 Impact Factor -
Article: Low prevalence of human papillomavirus (HPV) in Chinese patients with breast cancer.
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ABSTRACT: This retrospective study investigated the presence of human papillomavirus (HPV) in Chinese women with breast cancer, and the correlation between HPV infection and carcinogenesis. Tumour and non-cancerous breast tissue samples were obtained from 62 female patients with breast cancer; normal breast tissue samples were obtained from 46 women without breast cancer. HPV DNA was detected by nested polymerase chain reaction using consensus primers; HPV subtypes were determined by reverse dot blot and pyrosequencing analyses. HPV was found in tumour tissue samples from four of the 62 patients (6.5%), while no HPV DNA was detected in either the non-cancerous samples from patients with breast cancer or from the normal breast tissue controls. Of the four HPV-positive cases, three were HPV 16 positive (75%) and one was HPV 18 positive (25%). The low frequency of HPV detected in this study suggests that this infection is not a major risk factor in breast cancer development.The Journal of international medical research 01/2011; 39(5):1636-44. · 0.90 Impact Factor
