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ABSTRACT: The production in vitro of interferon alpha and gamma by peripheral blood mononuclear cells of 25 patients with chronic hepatitis B and 13 patients with chronic hepatitis non-A, non-B was compared to that of healthy controls. Following induction by Molt 4 leukemia cells (P less than 0.001) and influenza A/X31 virus (P less than 0.01), there was a significantly lower interferon alpha response in patients with chronic hepatitis B and chronic hepatitis non-A, non-B. Yields of interferon gamma in patients with chronic hepatitis were comparable to those of normal individuals. The degree of interferon deficiency did not correlate with severity of liver disease. In patients with chronic hepatitis B, viral replication (presence or absence of HBeAg) was not related to the defect in interferon alpha production. Three of 10 patients with acute hepatitis B had measurable antiviral activity in the serum for 3-5 days after the onset of jaundice.
Journal of Hepatology 07/1988; 6(3):364-8. · 9.26 Impact Factor
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ABSTRACT: Acute encephalopathy was associated with the presence of human immunodeficiency virus type I (HIV I) antigen and seroconversion to anti-HIV I in a 27-year-old homosexual man. Examination of consecutive sera from the patient revealed circulating interferon (IFN) alpha which became detectable with the appearance of HIV I antigen but before development of anti-HIV I. Serum IFN was present for only a limited time and was not demonstrable after neurological symptoms resolved. It may be speculated that circulating antiviral activity contributed to the clinical manifestations of acute HIV I infection.Der Nachweis von Human Immunodeficiency Virus Typ I (HIV I) Antigen und die Entwicklung von Anti-HIV I war bei einem 27 Jahre alten Homosexuellen mit akuter Enzephalopathie verbunden. Die Untersuchung von sequentiell entnommenen Patientenseren ergab, da zirkulierendes Interferon (IFN) alpha gleichzeitig mit dem Auftreten von HIV-I-Antigen, aber vor der Entwicklung von Anti-HIV I nachgewiesen werden konnte. Serum IFN alpha war nur fr einen begrenzten Zeitraum und nicht nach der Rckbildung der neurologischen Symptome nachweisbar. Die mgliche Beteiligung zirkulierender antiviraler Aktivitt an den klinischen Manifestationen der akuten Infektion mit HIV I wird diskutiert.
Infection 10/1987; 15(6):425-426. · 2.66 Impact Factor
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The Journal of Infectious Diseases 07/1986; 153(6):1174-5. · 6.41 Impact Factor
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European journal of clinical microbiology 07/1986; 5(3):365-8. · 2.61 Impact Factor
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European Journal of Clinical Microbiology 04/1986; 5(3):365-368. · 2.86 Impact Factor
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ABSTRACT: Peripheral blood leukocytes from patients with chronic hepatitis B virus (HBV) infection were studied for their capacity to produce interferon (IFN) alpha or IFN gamma. Yields of IFN alpha in leukocyte cultures stimulated with influenza A virus or human leukemic cells were significantly lower than those obtained from healthy controls. Production of IFN gamma in response to induction with protein A of Staphylococcus aureus was also significantly diminished. Defects of IFN production in leukocyte cultures showed no correlation with active viral replication or the degree of severity of HBV-associated liver disease. The demonstration of partial defects of endogenous IFN production provides a rationale for using IFN replacement therapy in patients with chronic HBV infection.
Journal of Medical Virology 07/1985; 16(2):171-6. · 2.82 Impact Factor
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ABSTRACT: HTLV III and LAV, retroviruses which have recently been described in the United States and France and which seem to be different isolates of the same virus, are closely associated with the acquired immune deficiency syndrome (AIDS) and the lymphadenopathy syndrome (LAS). Sera from male homosexuals from the Federal Republic of Germany with AIDS or LAS were examined for antibodies to HTLV III (anti-HTLV III) by means of the enzyme linked immunoadsorbent assay (ELISA) and by an indirect immunofluorescence assay. 53% of the patients were anti-HTLV III positive as were 20% of symptomless homosexual men.
DMW - Deutsche Medizinische Wochenschrift 12/1984; 109(45):1709-11. · 0.53 Impact Factor
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ABSTRACT: The relationship between NK active and IFN alpha-producing cells in human peripheral blood was studied with a monoclonal antibody with specificity for NK cells (anti-Leu11b). Removal of Leu11b antigen expressing leukocytes with antibody-mediated complement-dependent lysis resulted in a marked reduction of NK activity. In contrast, the depletion of Leu11b positive cells did not affect the production of IFN alpha in response to influenza A/X31 virus, Corynebacterium parvum, or Molt 4 human leukemic cells. The results indicate that NK activity and synthesis of IFN alpha are mediated by different leukocyte subpopulations. The findings further suggest that the augmentation of the cytotoxic activity of NK cells by IFN may not be the consequence of positive self-regulation, but rather of cellular cooperation.
Immunobiology 11/1984; 167(4):359-64. · 3.20 Impact Factor
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ABSTRACT: The influence of donor age on the production of interferon (IFN) alpha and IFN gamma by human peripheral blood mononuclear cells in vitro has been studied. The results demonstrate an age-related decline of the capacity to synthesize and secrete antiviral activity. Yields of virus-induced IFN alpha and lectin-induced IFN gamma were significantly decreased in mononuclear cell cultures from older subjects (greater than 50 years) when compared to those of younger subjects (less than 50 years). The observed deficiency of IFN production may be involved in the increased susceptibility of aged humans to viral infections and malignant diseases.
Blut 06/1984; 48(5):285-9.
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ABSTRACT: The influence of antigens of the major histocompatibility complex (MHC) on the production of interferon (IFN) alpha or IFN gamma by human peripheral blood leucocytes (PBL) in vitro has been studied. Synthesis of IFN gamma by PBL stimulated with purified phytohaemagglutinin (PHA-P) or protein A of Staphylococcus aureus (SpA) appeared not to be controlled by MHC antigens. The production of IFN alpha, however, was influenced by the HLA type of the donor. Low responsiveness of PBL to inducers of IFN alpha (influenza virus, Molt 4 cells) was associated with HLA-DR 2. Implications of these observations for studies of IFN production and natural killer (NK) cell activity are discussed.
Immunology 07/1983; 49(2):239-44. · 3.32 Impact Factor
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ABSTRACT: A dysfunction in natural killer (NK) cell activity has been assumed to play a substantial role in the pathogenesis of multiple sclerosis (MS). To investigate whether such a defect is genetically determined and thus in combination with a certain HLA status may represent an additional risk factor for contracting MS, spontaneous and interferon (IFN) induced NK cells activity against the K562 target cell were analyzed in nine pairs of monozygotic twins discordant for MS. In addition, IFN production was tested in nonadherent lymphocytes stimulated with PHA, influenza virus or leukemia cells. When compared to healthy controls, NK function appeared to be normal in healthy twins, whereas some MS patient displayed decreased activity. No difference in IFN induced NK cell activity and IFN production could be detected between normal controls, healthy twins, and MS patients. These data argue against a genetically determined dysfunction within the NK-IFN system in patients with MS.
Human Immunology 06/1983; 7(1):51-8. · 2.84 Impact Factor
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ABSTRACT: Monoclonal antibodies with specificities for subsets of human leucocytes have been used for the characterization of alpha-interferon (alpha-IFN) producing cells. The production of alpha-IFN was demonstrated to be a function of Ia+ leucocytes. OKT3+ T lymphocytes, BA-1+ B lymphocytes and Leu 7+ natural killer (NK) cells did not contribute to the production of alpha-IFN. OKM1+ monocytes were essential for the production of alpha-IFN in response to bacterial products or leukaemia cells, but were not required for the synthesis of virus- or poly I:C-induced alpha-IFN. The results indicate that alpha-IFN producing cells represent a heterogenous population of cells of the myeloid lineage.
Clinical & Experimental Immunology 05/1983; 52(1):179-84. · 3.36 Impact Factor
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ABSTRACT: Monoclonal antibodies with specificities for subsets of human leukocytes have been used for the characterization of interferon (IFN) gamma-producing cells. The production of IFN gamma was demonstrated to be a function of OKT3+T lymphocytes. The capacity to secrete IFN gamma was not restricted to the OKT4+ or the OKT8+T-cell subset. BA-1+B lymphocytes and Leu7+ natural killer cells did not contribute to the production of IFN gamma. Ia+, OKM1+ monocytes served an auxiliary function in the production of IFN gamma. The requirement for accessory monocytes, however, was not absolute, because monocyte-free preparations of long-term cultured IL2-dependent T lymphocytes retained the capacity to secrete IFN gamma.
Medical Microbiology and Immunology 02/1983; 171(4):215-23. · 3.83 Impact Factor
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ABSTRACT: Cyclosporin A (CsA) was assessed for its effect on the production of antiviral activity by human peripheral blood leukocytes (PBL). CsA markedly reduced the production of interferon-gamma (IFN-gamma) in response to stimulation with lectin mitogens, bacterial products, alloantigens, or Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL). CsA-mediated suppression of IFN-gamma secretion was dose-dependent and did not result from a shift of kinetics of the production of antiviral activity. The production of IFN-alpha in response to stimulation with Corynebacterium parvum (CP), viruses, and synthetic polynucleotides was not affected by the addition of CsA. These findings confirm earlier observations that CsA predominantly acts on T lymphocyte function. CsA may prove a valuable agent to study the role of IFN-gamma in the pathogenesis of virus-associated malignant lymphoproliferative disease.
Antiviral Research 01/1983; 2(6):361-7. · 4.30 Impact Factor
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ABSTRACT: Non-toxic concentrations of retinoic acid (RA) inhibited the spontaneous activity of human natural killer (NK) cells. RA also inhibited the activation of human NK cells by treatment with partially purified human leukocyte interferon (HulFN alpha) or with inducers of IFN alpha and IFN gamma. Full expression of the inhibitory action required prolonged exposure of human peripheral blood leukocytes to RA. Implications of these findings for the use of retinoids in the treatment of human malignancies are discussed.
International Journal of Cancer 10/1982; 30(3):307-10. · 5.44 Impact Factor
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J Abb,
F Deinhardt,
H Zander,
R B Tenser,
F Rapp,
J M Goust,
H H Fudenberg,
J Vilcek,
M Ho,
T C Merigan,
M B Oldstone,
G G Jackson
The Journal of Infectious Diseases 07/1982; 146(1):109-15. · 6.41 Impact Factor
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The Lancet 02/1982; 1(8266):280. · 38.28 Impact Factor
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ABSTRACT: We describe the production of marmoset lymphoblastoid cell interferon (IFN). Optimal yields of IFN were obtained when EBV-transformed lymphoblastoid cell lines (LCL) at a cell density of 1 X 10(6)/ml were incubated with 100 HAU Sendai virus/ml for 24 h. Sendai-virus-induced marmoset lymphoblastoid cell IFN was acid-stable and exerted antiviral activity on both homologous and heterologous human cells, thus allowing its classification as IFN alpha. Marmoset lymphoblastoid cell IFN did not inhibit the growth of herpesvirus-transformed marmoset T-or B-cell lines, but markedly enhanced marmoset NK-cell activity against human myeloid leukemia target cells. The use of lymphoblastoid cell IFN in marmosets in vivo may contribute to an understanding of the pathogenic role of IFN in herpesvirus-induced lymphoproliferative diseases.
International Journal of Cancer 01/1982; 29(1):77-80. · 5.44 Impact Factor
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The Journal of Infectious Diseases 09/1981; 144(2):179. · 6.41 Impact Factor
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Cellular Immunology 04/1980; 50(1):224-30. · 1.97 Impact Factor
