H Wu

Sichuan University, Chengdu, Sichuan Sheng, China

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Publications (11)11.18 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: This study reports the preliminary experience of dual grafts living donor liver transplantation (LDLT) for patients with acute-on-chronic liver failure (AoCLF) caused by hepatitis B. Two patients who demonstrated acute-on-chronic hepatitis B liver failure and portal hypertension with Model for End-Stage Liver Disease (MELD) scores of 42 and 37, respectively, underwent dual LDLT grafts including one right lobe without a middle hepatic vein and one left lobe because the graft-to-recipient body weight ratio of the right lobe grafts were 0.53% and 0.66%. The donors and the recipients have been followed for over 1 year. Mortality and operative complications were not observed in the donors or recipients. At present, the donors and recipients have returned to their daily routine. No prisoners or organs from prisoners were used to obtain these data. Dual LDLT grafts including one right lobe without the middle hepatic vein and one left lobe may be a possible therapeutic option for subjects with acute-on-chronic hepatitis B-induced liver failure.
    Transplantation Proceedings 12/2010; 42(10):4552-4. · 0.95 Impact Factor
  • L. Niu, Y. Xu, H. Wu
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    ABSTRACT: To attain a wear-resistant material compatible with high hardness and high toughness, Hadfield steel matrix was reinforced by oriented high chromium cast iron bars, through inserting high chromium alloys flux-cored welding wires into Hadfield steel melt at 1500 ± 10 °C. The obtained composites were investigated by XRD, SEM, micro-hardness, three-body abrasion wear and impact toughness testers. The results show that the alloy powders inside the flux-cored welding wires can be melted by the heat capacity of Hadfield steel melt and in situ solidified into high chromium cast iron bar reinforcements tightly embedded in the matrix. The micro-hardness of reinforcements of the water-quenched composite is about four times higher than that of the matrix. The impact toughness of the water-quenched composite is higher than that of the as-cast composite and lower than that of Hadfield steel, and its fracture mechanism is very complicated and refers to brittle and ductile mixture fracture mode. The excellent impact toughness and better wear resistance of the water-quenched composite are attributed to combine fully the advantages and avoid the drawbacks of both Hadfield steel and high chromium cast iron. Additionally, in industrial application, the pulverizer plate produced by this composite, has also better wear resistance compared to the reference Hadfield steel pulverizer plate.
    Fatigue & Fracture of Engineering Materials & Structures 11/2010; 34(6):423 - 429. · 0.86 Impact Factor
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    W Li, H Li, H Wu, X-Y Chen
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    ABSTRACT: Untranslated regions (UTRs) of eukaryotic mRNAs play crucial roles in post-transcriptional regulation of gene expression via the modulation of nucleocytoplasmic mRNA transport, translation efficiency, subcellular localization, and message stability. Single-nucleotide polymorphisms (SNPs) in UTRs of a candidate gene may also change the post-transcriptional regulation of a gene or function by nucleotide mutation. For species that have not been entirely sequenced genomically, new methods need to be devised to discover SNPs in noncoding regions of candidate genes. In this study, based on the expressed sequence tag (EST) of Pinus radiata (Monterey pine), we obtained all the sequences of UTRs of the actin gene by using a chromosome walking method. We also detected all the SNPs in and around the coding region of the actin gene. In this way, the full genomic sequence (2154 bp) of the actin gene was identified, including the 5’UTR, introns, the coding sequence, and the 3’UTR. PCR amplification and DNA fragment sequencing from 200 unrelated P. radiata trees revealed a total of 21 SNPs in the actin gene, of which 3 were located in the 5’UTR, 3 in the introns, 10 in the coding sequence, and 5 in the 3’UTR. We show that chromosome walking can be used for obtaining the sequence of UTRs, and then, based on this sequence, to discover SNPs in the noncoding regions of candidate genes from this species without an entire genomic sequence.
    Journal of applied genetics 09/2010; 51(3):275-81. · 1.90 Impact Factor
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    ABSTRACT: This cross-sectional study investigated potential factors impacting quality of life in 125 recipients after living-donor liver transplantation (LDLT). Health-related quality of life (HRQoL) was measured by using the Chinese version of Medical Outcomes Study Short Form-36 (SF-36), and psychologic symptoms by using the Symptom Checklist-90-Revised (SCL-90-R). Clinical and demographic data were collected from the records of the Chinese Liver Transplant Registry and via questionnaire. A total of 102 recipients (81.6%) completed the questionnaires. All SF-36 domain scores (except the mental health score) were lower in the study than in the general population of Sichuan. The mental quality of life was significantly lower in female than in male subjects (P = .000). Regarding the role-physical (P = .016), social functioning (P = .000), and role-emotional (P = .004) domains, recipients >1 year after transplantation scored higher than those <1 year. Bodily pain scores were lower in recipients with prior acute liver failure than those with hepatic carcinoma or hepatic cirrhosis (P = .032). Social functioning was poorer in recipients with than in those without complications (P = .039). Mental component summary scale (MCS) scores and some of physical component summary scale (PCS) significantly correlated with symptom dimension scores of the SCL-90-R (P < .05). In conclusion, gender, time since transplant, etiology of disease, complications, occupation, and some psychologic symptoms were possible factors influencing postoperative HRQoL of LDLT recipients.
    Transplantation Proceedings 09/2010; 42(7):2611-6. · 0.95 Impact Factor
  • L. Niu, Y. Xu, H. Wu, W. Wang
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    ABSTRACT: Using chromium wires as starting material, an iron based composite coating reinforced by in situ (Fe,Cr)7C3 particles, with a thickness of 4?0 mm, was fabricated on the surface of the ductile cast iron substrate, by means of a centrifugal casting technique. The microstructure and properties of the composite coating were investigated by optical microscope, X-ray diffraction, scanning electron micrograph, energy dispersive spectrum, microhardness tester and pin on disc wear tester. Results show that the composite coating consists of primary (Fe,Cr)7C3 carbides as the reinforcing phase and interdendritic (Fe,Cr)7C3/? eutectics accompanied by negligible graphite nodules as the matrix. The composite coating is metallurgically bonded to the ductile cast iron substrate. Under the condition of dry sliding with loads, the composite coating offers virtually a unique wear resistant behaviour compared with that of unreinforced ductile cast iron material.
    Surface Engineering 01/2010; 26(6). · 1.51 Impact Factor
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    ABSTRACT: Most reported data on posttransplantation diabetes mellitus (PTDM) are from Western countries with patients who underwent deceased donor liver transplantation. A retrospective study was performed to assess the prevalence and predictive factors of PTDM in the context of living donor liver transplantation (LDLT) in the Chinese population using the definition of PTDM proposed in 2003 by the World Health Organization and the American Diabetes Association. The prevalence of DM after LDLT in our study was 25% (21/84), and the incidence of PTDM was 14.9% (11/74) with 64% of cases diagnosed within 3 months after LDLT; 9.5% were observed to show impaired fasting glucose postoperatively. Multivariate analysis identified body mass index >or= 25 kg/m(2) before LDLT as the only independent risk factor for developing PTDM. Only one patient was operated for hepatitis C virus (HCV) infection. Hepatitis B virus (HBV)-related diseases were common in our study population, accounting for 78.6% of all patients. Both HCV and HBV infection status were not independent risk factors for developing PTDM. In addition, a greater tacrolimus trough blood level in the PTDM group versus no-DM group was observed at 3 months post-LDLT (11.03 ng/mL vs 4.87 ng/mL). The mean tacrolimus dose was not significantly different between the two groups. In conclusion, PTDM was prevalent among Chinese LDLT recipients.
    Transplantation Proceedings 06/2009; 41(5):1756-60. · 0.95 Impact Factor
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    ABSTRACT: The aim of this study was to explore the indications for liver transplantation among patients with hepatolithiasis. Data from 1,431 consecutive patients who underwent surgical treatment from January 2000 to December 2006 were retrospectively collected for analysis. Surgical procedures included T-tube insertion combined with intraoperative cholangioscopic removal of intrahepatic stones, hepatectomy, cholangiojejunostomy, and liver transplantation. Nine hundred sixty-one patients who had a stone located in the left or right intrahepatic duct underwent hepatectomy or T-tube insertion combined with intraoperative cholangioscopic removal of intrahepatic stones. The rate of residual stones was 7.5%. Four hundred seventy patients who had a stone located in the bilateral intrahepatic ducts underwent surgical procedures other than liver transplantation; the rate of residual stones was 21.7%. Only 15 patients with hepatolithiasis underwent liver transplantation; they all survived. According to the degree of biliary cirrhosis, recipients were divided into 2 groups: a group with biliary decompensated cirrhosis (n = 7), or group with compensated cirrhosis or no cirrhosis (n = 8). There were significant differences in operative times, transfusion volumes, and blood losses between the 2 groups (P < .05). In the first group, 6 of 7 patients experienced surgical complications, and in the second, 8 recipients recovered smoothly with no complications. Health status, disability, and psychological wellness of all recipients (n = 15) were significantly improved at 1 year after transplantation compared with pretransplantation (P < .05). Liver transplantation is a possible method to address hepatolithiasis and secondary decompensated biliary cirrhosis or difficult to remove, diffusely distributed intrahepatic duct stones unavailable by hepatectomy, cholangiojejunostomy, and choledochoscopy.
    Transplantation Proceedings 12/2008; 40(10):3517-22. · 0.95 Impact Factor
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    ABSTRACT: To investigate the protective effects of pirfenidone on acute liver damage caused by D-galactosamine (GalN)/lipopolysaccharide (LPS) in rats. Sprague-Dawley rats were divided into five groups (five rats per group): normal control group, GalN/LPS-treated group, and three pirfenidone-treated group (100, 300 and 500 mg/kg i.p., respectively). All biochemical and histological indexes were determined at 12 h after GalN/LPS challenge. Severity of liver injury was assessed by determination of serum ALT, AST levels and histological analysis. SOD activity and MDA concentrations as well as TNF-alpha and IFN-gamma levels in the liver of rats were measured. The expression of iNOS and its product, NO concentration were also determined. Pretreatment with pirfenidone significantly attenuated GalN/LPS-induced severe hepatotoxicity, as evidenced by decreased ALT, AST levels and MDA content and improved histopathological changes. Pirfenidone inhibited the elevated levels of TNF-alpha and IFN-gamma and reduced the induction of iNOS/NO in a dose-dependent manner, which might be important mechanisms related to its protective effect. Pirfenidone can provide a definite protective effect against acute hepatic injury caused by GalN/LPS in rats, which may be mainly mediated through its anti-inflammatory effect.
    Inflammation Research 04/2008; 57(4):183-8. · 2.14 Impact Factor
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    ABSTRACT: High rates of biliary complications continue to be a major concern associated with living donor liver transplantation (LDLT). In this article, we report our experience of applying a microsurgical technique to biliary reconstruction in LDLT. From January 2001 to December 2005, 32 patients underwent LDLTs (8 children and 24 adults). Biliary reconstruction for 43 hepatic duct orifices in the 32 donor grafts 21 duct-to-duct anastomoses, and 22 cholangiojejunostomies. Nine cholangiojejunostomies in 4 donors used a microsurgical technique under an operative microscope. Biliary complications weren't observed among the cases of cholangiojejunostomy using a microsurgical technique. An anastomotic biliary leakage was found in a recipient with cholangiojejunostomy performed using a surgical loupe and a biliary stricture in another recipient who underwent duct-to-duct anastomoses using a surgical loupe. Introduction of a microsurgical technique for biliary reconstruction in LDLT, especially using an operating microscope in the setting of hepatico-jejunostomy for small hepatic duct (< or =2 mm in diameter), showed good results. We believe that using the operative microscope for biliary reconstruction could reduce the incidence of biliary complications associated with LDLT.
    Transplantation Proceedings 07/2007; 39(5):1513-6. · 0.95 Impact Factor
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    ABSTRACT: CCSD logging engineering gather many modern high technologies and employs various advanced logging tools to survey the sidewall continuously. This can obtain various physical, chemical, geometrical, etc in-situ information of the borehole's profile. So well logging is one of the most important parts and pivotal technologies in the project of CCSD. The main logging methods in CCSD-MH(0-2000m) are laterolog (Rd,Rs), gamma ray(GR), nature gamma spectrometry(U, TH, K), density(DEN), photo electric section exponent (Pe), compensated neutron(CNL), multipole array acoustic (Vp, Vs, Vst), Simultaneous Acoustic-Resistivity-image(Star-II), temperature(T),magnetic susceptibility(MS), three component borehole magnetic and redox potential log,etc. The various metamorphic rocks can be classified by logging curves,and their physical parameters can be acquired by analyzing the response characters of various metamorphic rocks and by statistics. According to the logging cross plot, We can research the clustering of metamorphite's physical property. Five lithologic segments can be obtainend by logging curves. The GR, Th, U, K logging values of segment 1 is lower than the third, fourth and fiveth segment, higher than segment 2; The DEN, Pe values of segment 1 higher than the third, fourth and fiveth segments. The main rocks in segment 1,2,3,4,5 are eclogites, serpentinites, paragneiss, orthogneiss, and eclogites(containing silicon and muscovite ) respectively. Generally, eclogite contain rutile, silicon, muscovite, etc. minerals. These minerals have response obviously on log curves.There are rutile,ilmenite, pyrite mineralized, etc. Making use of DEN, Pe, susceptibility log values, these mineralized layers can be goodly demarcation. For example, on the rutile mineralzed layer, the logging curve response characters are of high density and Pe obviously. The key data of the synthetical seismic record is wave impedance. In this paper, Utilize the data of AC, DEN curves to calculate the wave impedance and compare with the VSP profile, finally analyze the reflectors of the CCSD-MH. Imaging log has a positioning system and very good vertical resolution, and can describe the geological features in detail. Various structure parameters (the size and occurrences of foliation, fracture, fault & vein) have been given by image logging. The main work of logging interpretation of CCSD have done as follows: 1) Character analysis of logging response and restoring the lithologic profile;2) Depth correction and Restoring orientation of cores; 3) Interpretation of imaging geological feature; 4) Research of rock's mechanics character, sonic anisotropy and formation stress; 5) Comparison wave impedance with the VSP profile,analyzing the reflectors; 6)The interpretation of magnetic susceptibility and temperature log. The logging analysis results of UHP metamorphic rocks in CCSD-MH(0-2000m) show that responses of logs curve are abundant, the physical properties of various metamorphic rocks are visibly different;image logging has a positioning system and very good vertical resolution, and can describe the geological features in detail;lithologic segments and mineralized layers can be goodly demarcation by log curves;the log curves can be used for standardizing the geophysical survey, for example, mark out the reflected interface of seismic wave;comparing the results of log curves value with core laboratory analysis,they have good consistency;and so on.
    AGU Fall Meeting Abstracts. 12/2004;
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    ABSTRACT: A principle of optical path adjustment by servo-control mechanism is proposed. A computer program based on this principle has been successfully used in the auto-alignment of a laser system. A large laser system usually consists of an oscillator, a number of amplifiers and extensive optics. While in operation it is difficult to keep the laser beam from drifting. For example, the optical path of the pulsed solid state laser system used in nuclear fusion relies on an alignment control system so that the axial and angular drifts of the beams remain within a tolerable range. In this article we report our research results in this area.

Publication Stats

48 Citations
11.18 Total Impact Points

Institutions

  • 2008
    • Sichuan University
      • Department of General Surgery
      Chengdu, Sichuan Sheng, China
  • 2007
    • West China School of Medicine
      Hua-yang, Sichuan, China