Hiroyuki Arai

Publications (2)9.55 Total impact

• Article: A Novel Role for hGas7b in Microtubular Maintenance

  Hirotada Akiyama, Aina Gotoh, Ryong-Woon Shin, Tomoe Koga, Tsubasa Ohashi, Wataru Sakamoto, Akihiro Harada, Hiroyuki Arai, Akira Sawa, Chiyoko Uchida, Takafumi Uchida
  [show abstract] [hide abstract]
  ABSTRACT: Here, we report a novel role for hGas7b (human growth arrest specific protein 7b) in the regulation of microtubules. Using a bioinformatic approach, we studied the actin-binding protein hGas7b with a structural similarity to the WW domain of a peptidyl prolyl cis/trans isomerase, Pin1, that facilitates microtubule assembly. Thus, we have demonstrated that hGas7b binds Tau at the WW motif and that the hGas7b/Tau protein complex interacts with the microtubules, promoting tubulin polymerization. Tau, in turn, contributes to protein stability of hGas7b. Furthermore, we observed decreased levels of hGas7b in the brains from patients with Alzheimer disease. These results suggest an important role for hGas7b in microtubular maintenance and possible implication in Alzheimer disease.
  Journal of Biological Chemistry 11/2009; 284(47):32695-32699. · 4.77 Impact Factor
• Article: A novel role for hGas7b in microtubular maintenance: possible implication in tau-associated pathology in Alzheimer disease.

  Hirotada Akiyama, Aina Gotoh, Ryong-Woon Shin, Tomoe Koga, Tsubasa Ohashi, Wataru Sakamoto, Akihiro Harada, Hiroyuki Arai, Akira Sawa, Chiyoko Uchida, Takafumi Uchida
  [show abstract] [hide abstract]
  ABSTRACT: Here, we report a novel role for hGas7b (human growth arrest specific protein 7b) in the regulation of microtubules. Using a bioinformatic approach, we studied the actin-binding protein hGas7b with a structural similarity to the WW domain of a peptidyl prolyl cis/trans isomerase, Pin1, that facilitates microtubule assembly. Thus, we have demonstrated that hGas7b binds Tau at the WW motif and that the hGas7b/Tau protein complex interacts with the microtubules, promoting tubulin polymerization. Tau, in turn, contributes to protein stability of hGas7b. Furthermore, we observed decreased levels of hGas7b in the brains from patients with Alzheimer disease. These results suggest an important role for hGas7b in microtubular maintenance and possible implication in Alzheimer disease.
  Journal of Biological Chemistry 09/2009; 284(47):32695-9. · 4.77 Impact Factor