Ingrid Capesius

Universität Heidelberg, Heidelburg, Baden-Württemberg, Germany

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Publications (16)39.11 Total impact

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    ABSTRACT: RNA editing affects messenger RNAs and transfer RNAs in plant mitochondria by site-specific exchange of cytidine and uridine bases in both seed and nonseed plants. Distribution of the phenomenon among bryophytes has been unclear since RNA editing has been detected in some but not all liverworts and mosses. A more detailed understanding of RNA editing in plants required extended data sets for taxa and sequences investigated. Toward this aim an internal region of the mitochondrial nad5 gene (1104 nt) was analyzed in a large collection of bryophytes and green algae (Charles). The genomic nad5 sequences predict editing in 30 mosses, 2 hornworts, and 7 simple thalloid and leafy liverworts (Jungermanniidae). No editing is, however, required in seven species of the complex thalloid liverworts (Marchantiidae) and the algae. RNA editing among the Jungermanniidae, on the other hand, reaches frequencies of up to 6% of codons being modified. Predictability of RNA editing from the genomic sequences was confirmed by cDNA analysis in the mosses Schistostega pennata and Rhodobryum roseum, the hornworts Anthoceros husnotii and A. punctatus, and the liverworts Metzgeria conjugata and Moerckia flotoviana. All C-to-U nucleotide exchanges predicted to reestablish conserved codons were confirmed. Editing in the hornworts includes the removal of genomic stop codons by frequent reverse U-to-C edits. Expectedly, no RNA editing events were identified by cDNA analysis in the marchantiid liverworts Ricciocarpos natans, Corsinia coriandra, and Lunularia cruciata. The findings are discussed in relation to models on the phylogeny of land plants.
    Journal of Molecular Evolution 04/1999; 48(3):303-12. · 2.15 Impact Factor
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    10/1998;
  • K. M. F.  WARPEHA , I.  CAPESIUS 
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    ABSTRACT: To date, little molecular genetic research has been conducted in Lolium and the current study is the first investigation of diversity in ribosomal DNA (rDNA) among perennial ryegrass cultivars. Ribosomal DNA probes derived from the intergenic spacer (IGS–M) and transcribed (COD–M) regions of mustard (Sinapis alba) were used to examine 35 perennial ryegrass (L. perenne L.) cultivars for genetic diversity using RFLP methodology. Analysis of digested genomic DNA on Southern blots revealed only 10 cultivars with unique RFLP patterns. Genetic diversity was exclusively associated with the IGS–M probe, as the cultivars were all indistinguishable when examined with the COD–M probe. This low incidence of distinctions was not expected, given that the 35 cultivars were known genetically distinct entities, representing the very wide range of the major phenotypic and physiological characteristics present in commercial stocks. Furthermore, the forage cultivars were proportionately less polymorphic in comparison to the amenity cultivars. This was also unexpected as amenity cultivars are recognized as a more closely clustered group than the forage cultivars, based on morphological characteristics. The potential use of rDNA for assessing cultivar diversity and breeding histories in perennial ryegrass is considered.
    The Journal of Agricultural Science 07/1998; 131(01):23 - 30. · 2.88 Impact Factor
  • I Capesius
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    ABSTRACT: Ribosomal DNA from the moss Funaria hygrometrica was obtained by centrifugation of genomic DNA in a Hoechst 33258-CsCl density gradient. One of the three DNA-satellite bands (GC2) represented the ribosomal fraction. After digestion with EcoRI three larger fragments were obtained which hybridized to the large and small ribosomal subunits from Sinapis alba. The fragments were cloned and sequenced. The complete ribosomal DNA consists of 11,132 bp. The analysis of the 18S, 5.8S and 25S rDNA revealed a high sequence and length similarity to other plant sequences. An intron of 169 bp situated 10 bp from the 5' end of the 25 S rDNA was found. The intergenic spacer (IGS) has a length of 5150 bp with 13 highly conserved subrepeats (38 bp except for two of 56 bp) followed directly by 6 other repeated elements of 42-43 bp. Two putative transcription initiation sites (TIS) were identified with some differences to known TIS of angiosperms.
    Plant Molecular Biology 03/1997; 33(3):559-64. · 3.52 Impact Factor
  • Ingrid Capesius, Martin Bopp
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    ABSTRACT: Complete sequences for the 18S-rRNA gene of 22 bryophytes (12 completely new) were determined and used to construct phylogenetic trees. The evaluation of sequence data according to the maximum parsimony principle (PAUP 3.1.1) and the neighbor-joining method (MEGA) results in similar phylogenetic trees in which theBryopsida appear as a sister group to theJungermanniopsida, and both together as a sister group to theMarchantiopsida. Among theMarchantiopsida, theSphaerocarpales diverge early as a separate clade. TheMetzgeriales andJungermanniales are monophyletic. They belong to one clade and cannot be separated by either method of evaluation.
    Plant Systematics and Evolution 01/1997; 207(1):87-97. · 1.31 Impact Factor
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    ABSTRACT: Complete nuclear-encoded small-subunit 18S rRNA (= SSU rRNA) gene sequences were determined for the prasinophyte green alga Mantoniella squamata; the charophycean green algae Chara foetida, Coleochaete scutata, Klebsormidium flaccidum, and Mougeotia scalaris; the bryophytes Marchantia polymorpha, Fossombronia pusilla, and Funaria hygrometrica; and the lycopod Selaginella galleottii to get a better insight into the sequential evolution from green algae to land plants. The sequences were aligned with several previously published SSU rRNA sequences from chlorophytic and charophytic algae as well as from land plants to infer the evolutionary relationships for major evolutionary lineages within the Chlorobionta by distance matrix, maximum parsimony, and maximum likelihood analyses. Phylogenetic trees created by the different methods consistently placed the Charophyceae on the branch leading to the land plants. The Charophyceae were shown to be polyphyletic with the Charales ("charalean" algae) diverging earlier than the Coleochaetales, Klebsormidiales, Chlorokybales, and Zygnematales ("charophycean" algae) which branch from a point closer to the land plants in most analyses. Maximum parsimony and maximum likelihood analyses imply a successive evolution from "charophycean" algae, particularly Coleochaetales, to bryophytes, lycopods, and seed plants. In contrast, distance matrix methods group the bryophytes together with the "charophycean" algae, suggesting a separate evolution of these organisms compared with the club moss and the seed plants.
    Journal of Molecular Evolution 08/1995; 41(1):74-84. · 2.15 Impact Factor
  • Ingrid Capesius
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    ABSTRACT: Complete small ribosomal 18S RNA gene sequences from 6 liverworts, 6 mosses and 1 hornwort were used to reconstruct phylogenetic relationships between bryophytes. Sequence data were evaluated according to the maximum parsimony principle (PAUP 3.1.1) and the neighbour-joining method (MEGA). As outgroup, Chara foetida was used. For the first time molecular data support the open discussion about the relationship between Marchantiidae and Jungermanniidae liverworts, placing them regardless of the method used in different clades. The greatest absolute distances are found between Chara foetida and Marchantiidae followed by Sphagnum and Anthoceros. Chara foetida is closest to the Bryidae and Jungermanniidae.
    Journal of Plant Physiology. 05/1995; 146(s 1–2):59–63.
  • Ingrid Capesius
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    ABSTRACT: 5S rDNA was obtained from Brassica nigra by the polymerase chain reaction using total nuclear DNA, was cloned in pUC 18 vector and sequenced. At the 5S rRNA level the homology to other representatives of the same family was between 97% and 100%. The degree of homology over the gene and spacer level between Brassica nigra and Sinapis alba, which are very closely related, is 85–86%. Conserved sequence elements of the 5S rRNA genes including the TATA box at position -28 to -25 upstream to the transcription point among Brassicaceae were found.
    Journal of Plant Physiology. 07/1993; 142(1):112–114.
  • I Capesius
    Plant Molecular Biology 08/1991; 17(1):169-70. · 3.52 Impact Factor
  • I Capesius
    Plant Molecular Biology 07/1991; 16(6):1093-4. · 3.52 Impact Factor
  • Ingrid Capesius
    Plant Molecular Biology 06/1991; 17(1):169-170. · 3.52 Impact Factor
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    J Rathgeber, I Capesius
    Nucleic Acids Research 04/1990; 18(5):1288. · 8.28 Impact Factor
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    J Rathgeber, I Capesius
    Nucleic Acids Research 10/1989; 17(18):7522. · 8.28 Impact Factor
  • Ingrid Capesius
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    ABSTRACT: Leaves of Lobularia maritima floating on 0,2 M NaCl show a greater increase in fresh weight than leaves on water. The synthesis of nucleic acids — studied by incorporation of 3H-Thymidin and 32P-Phosphate — was lower on NaCl than on H2O. The synthesis of DNA and RNA was strongly inhibited by 8 · 10−5 M FdUrd; during a experimental duration of 21 hours DNA-synthesis was inhibited by 90 %, RNA by 67 %. In 21 hours fresh weight is not influenced by FdUrd. The lag phase of the FdUrd effect on DNA synthesis was about 4–8 hours.
    Zeitschrift für Pflanzenphysiologie. 08/1974; 73(3):266–272.
  • Ingrid Capesius
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    ABSTRACT: The enzyme activity of thymidine kinase and thymidylate kinase was studied in seedlings of Sinapis alba during 29 to 90 h after sowing. The maximum of the enzyme activities is reached later than that of DNA synthesis. Addition of FdUrd or thymidine 40 h after sowing results in an increase of the activity of thymidine kinase within the hypocotyls. On the other hand the activity of thymidylate kinase was decreased by addition of FdUrd by 30 to 57%. This inhibition of the enzyme activty could be overcome by simultaneous addition of thymidine. A gradient of the activities of the two enzymes decreasing from the hypocotyl hook to the base was found.
    Zeitschrift für Pflanzenphysiologie. 05/1973; 69(5):438–446.
  • Ingrid Capesius, Yves Van de Peer
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    ABSTRACT: In this study, the complete nucleotide sequence of the large ribosomal subunit RNA of the bryophyte Funaria hygrometrica was determined. The RNA sequence, which is the first one reported for bryophytes, was used to infer a secondary structure model. It delivers the base for further evolutionary studies in this group.
    Journal of Plant Physiology. 151(2):239–241.