Ingrid Capesius

Universität Heidelberg, Heidelburg, Baden-Württemberg, Germany

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Publications (22)37.83 Total impact

  • I. Capesius, M. Bopp, K. Nishitani, Y. Masuda
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    ABSTRACT: Etiolated seedlings of Sinapis alba treated for 24 hours with 40 µM FdUrd become inhibited in elongation growth.64 hours old seedlings only reach the same length as untreated plants of 48 hours.A comparison of untreated seedlings of 48 and 64 hours with inhibited plants of 64 hours gives the following results: The minimum stress relaxation time is the same for seedlings of the same size, whereas the extensibility is more dependent on the seedling’s age.The total cell wall material content of the treated plants lies between the two controls, but the composition of the wall material (pectin and hemicelluloses) is not significantly changed by the treatment, only arabinose is significantly enhanced.From these results it can be concluded that FdUrd does not inhibit the growth by changing the formation or composition of the cell wall.The reduction of wall material is rather a consequence of the reduced growth.
    Zeitschrift für Pflanzenphysiologie. 08/1981; 103(1):87–93.
  • Ingrid Capesius
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    ABSTRACT: The DNA from mustard (Sinapis alba L.) seedlings was examined by neutral CsCl and Ag+/Cs2SO4 density gradient centrifugation. Different satellite fractions were revealed by these two methods. The satellite fractions obtained from the Ag+/Cs2SO4 density gradient could not be generally correlated with satellite DNA fractions observed in CsCl. In CsCl density gradient centrifugation, a main band at density 1,695 g/cm3 and a heavy shoulder at density 1,703 g/cm3 are found. By preparative CsCl gradient centrifugation the heavy shoulder can be enriched but not completely separated from the main band DNA.—Gradient centrifugation by complexing the DNA with Ag+ rf. 0.25 to DNA phosphate reveals three distinct fractions which are further characterized: The heavy satelite DNA fraction revealed by Ag+/Cs2SO4 gradient centrifugation has the same density in a CsCl gradient and the same Tm value as the main band, but differs from main band DNA in the details of its melting profile and in its renaturation kinetics. The light Ag+/Cs2SO4 satellite DNA fraction had a higher melting temperature corresponding to a GC-rich base composition. Differences between these 3 fractions are observed in thermal denaturation and renaturation profiles, hybridization in situ with ribosomal RNA, and their response to restriction endonuclease digestion. The light satellite fraction from the Ag+/Cs2SO4 gradient, rich in ribosomal cistrons corresponds to the heavy shoulder DNA of neutral CsCl gradients which also is rich in ribosomal cistrons. The heavy satellite fraction from Ag+/Cs2SO4 gradient which contains highly repetitive short nucleotide sequences could not be revealed by the classical CsCl gradient centrifugation technique.
    Plant Systematics and Evolution 01/1979; 133(1):1-13. · 1.31 Impact Factor
  • Ingrid Capesius, Walter Nagl
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    ABSTRACT: The species- and genus-specific DNA content, average base composition of nuclear DNA, presence or absence of satellite DNA, the percentage of heterochromatin and other characteristics of nuclear DNA and nuclear structure allow to deduce the molecular changes which accompanied, or more probably caused, cladogenesis in the orchids studied. It is suggested that saltatory replication (generative amplification) of certain DNA sequenes, diversification of reiterated DNA sequences, and loss of DNA play an important role in the evolution of orchids.—The relationship between changes of genome composition and of nuclear structure and ultrastructure is discussed on the basis of cot curves, heterochromatin staining with Giemsa (C banding), electron microscopy of nuclei, and molecular hybridization in situ.
    Plant Systematics and Evolution 08/1978; 129(3):143-166. · 1.31 Impact Factor
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    I Capesius
    FEBS Letters 11/1976; 68(2):255-8. · 3.58 Impact Factor
  • W. Nagl, I. Capesius
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    ABSTRACT: As a contribution for the study of systematic and evolutionary relationships it is suggested to analyze nuclear DNA and chromatin by means of CsCl ultracentrifugation, thermal denaturation and renaturation, scanning densitometry, and (ultra)structural analyses. Relevant data have been obtained forHelianthus annuus as a first example.The 2C DNA content of four cultivars ofHelianthus annuus L. was calibrated by comparative measurement withAllium cepa nuclei using a scanning densitometer in on-line operation with a computer. Significant infraspecific variation could be detected: cvar. Amerikanische Riesen displayed 6.1 pg, cvar. Gefllte Vielbltige 9.9 pg, cvar. Russian Mammoth 8.9 pg, and a Heidelberg strain 8.7 pg.The buoyant density in neutral CsCl was determined for cvar. Amerikanische Riesen to be 1.695 g cm–3; this corresponds to an average GC content of 35.1%. Thermal denaturation revealed a melting temperature of 86.4C. Derivative thermal denaturation profiles led to the detection of several distinct DNA fractions.The species-specific nuclear structure is of the chromonematic type, but in differentiated cells the chromatin fibers may be more decondensed so that a chromomere-interchromomere structure appears. The heterochromatin constitutes an average of 4.5% of the total genome. Chromatin ultrastructure is characterized by a diffuse distribution of chromatin threads and patches. Nucleosomes of 110 diameter can be recognized.The data are discussed (a) in relation to findings on DNA variation in other plants, (b) in relation to the systematic usefulness and further characterization of nuclear DNA and chromatin, and (c) in relation to tissue-specific and functional variation of the species-specific chromatin structure.
    Plant Systematics and Evolution 05/1976; 126(2):221-237. · 1.31 Impact Factor
  • W. Nagl, I. Capesius
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    ABSTRACT: Endopolyploidy has been detected in some varieties ofHelianthus annuus L. (Asteraceae/Compositae) by means of scanning photometry of Feulgen-stained nuclei and analysis of nuclear structure. In the hypocotyl cells of seedlings, ploidy levels reach respectively 8 C and 16 C in different varieties, in the root cells 8 C and 16 C; in the cotyledons of ripening seeds 4 C to 8 C values have been found, while all nuclei of the inflorescence axis of one variety exhibit a DNA content of 4 C.—This is the first report of endopolyploidy in a non-succulentAsteraceae species. The characteristic distribution of the endopolyploidy levels in different varieties suggests a strong genetic and/or hormonal control of the final nuclear DNA content in differentiated cells.
    Plant Systematics and Evolution 01/1976; 125(4):261-268. · 1.31 Impact Factor
  • W. Barthlott, I. Capesius
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    ABSTRACT: Zusammenfassung 1. Das Velamen radicum tropischer Orchidaceae aus 17 Gattungen wird raster-elektronenmikroskopisch untersucht; durch Markierung mit tritiiertem Wasser wird die Absorptionsfähigkeit der Luftwurzeln von sieben Gattungen geprüft.2. Die Wände aller Velamenzellen der untersuchten Orchidoideae sind mit regelmäßigen Wandleisten und nativen Poren ausgestattet. Der räumliche Verlauf der Leisten und davon abhängig Verteilung und Größe der Wanddurchbrechungen wird genauer dargestellt und durch REM-Aufnahmen illustriert. Es lassen sich mehrere Variationen der Strukturierungsmuster von Velamenzellen finden, die vermutlich taxonspezifisch sind.3. Die Isotopenmarkierungen ergeben, daß das vom Velamen der Luftwurzeln bei Acampe, Epidendrum, Microcoelia und Rhynchostylis aufgenommene Wasser dem lebenden Gewebe zugeführt wird. Dagegen haben die dünnen, geotrop kaum beeinflußten Luftwurzeln der heterorhizen Gattungen Ansellia, Catasetum und Graphorkis nichts mit der Funktion der Wasseraufnahme zu tun.
    Plant Biology 10/1975; 88(3). · 2.32 Impact Factor
  • I. CAPESIUS, W. BARTHLOTT
    Z. Pflanzenphysiologie. 07/1975; 75(5):436-448.
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    ABSTRACT: The DNA of aseptically grown protocorms of a Cymbidium hybrid and in vitro developed leaves, as well as DNA of leaves and flower buds of Cymbidium ceres from the greenhouse, was analysed by analytical ultracentrifugation and thermal denaturation. Upon ultracentrifugation a satellite DNA with a buoyant density of 1.682 g/cm-3 appears as a shoulder on the main band (density 1.694 g/cm-3). Thermal denaturation reveals an inhomogeneous main peak with the major component melting at 84 degrees C and a separate peak melting at 75 degrees C. This is the first demonstration of a satellite DNA in a monocot, and one of the rare examples of a major A + T-rich DNA fraction in a plant.
    Biochimica et Biophysica Acta 07/1975; 395(1):67-73. · 4.66 Impact Factor
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    ABSTRACT: The DNA of aseptically grown protocorms of a Cymbidium hybrid and in vitro developed leaves, as well as DNA of leaves and flower buds of Cymbidium ceres from the greenhouse, was analysed by analytical ultracentrifugation and thermal denaturation. Upon ultracentrifugation a satellite DNA with a buoyant density of 1.682 g/cm3 appears as a shoulder on the main band (density 1.694 g/cm3). Thermal denaturation reveals an inhomogeneous main peak with the major component melting at 84°; C and a separate peak melting at 75°; C. This is the first demonstration of a satellite DNA in a monocot, and one of the rare examples of a major DNA fraction in a plant.
    Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 06/1975; 395(1):67–73.
  • Ingrid Capesius
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    ABSTRACT: Leaves of Lobularia maritima floating on 0,2 M NaCl show a greater increase in fresh weight than leaves on water. The synthesis of nucleic acids — studied by incorporation of 3H-Thymidin and 32P-Phosphate — was lower on NaCl than on H2O. The synthesis of DNA and RNA was strongly inhibited by 8 · 10−5 M FdUrd; during a experimental duration of 21 hours DNA-synthesis was inhibited by 90 %, RNA by 67 %. In 21 hours fresh weight is not influenced by FdUrd. The lag phase of the FdUrd effect on DNA synthesis was about 4–8 hours.
    Zeitschrift für Pflanzenphysiologie. 08/1974; 73(3):266–272.
  • W. BARTHLOTT, I. CAPESIUS
    Z. Pflanzenphysiologie. 06/1974; 72(5):443-455.
  • I. Capesius, M. Bopp
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    ABSTRACT: Etiolated elongating hypocotyls of Sinapis alba synthesize DNA 40–72 h after sowing. The increase in DNA content is about 62.5%. Four-hour pulse experiments showed an increasing rate of [32P]orthophosphate incorporation; the maximum rate was obtained 64–68 h after sowing. In the cotyledons DNA increase up to the 56th h after sowing; later the DNA-content remains constant. During this time the incorporation of 32P into the DNA of the cotyledons decreases continuously.After simultaneous application of Fluordesoxyuridin (FdUrd) (410-5M) and thymidine (410-4M) or thymidylate (410-4M) 40–64 h after sowing, the growth rate was the same as that in H2O treated controls, whereas application of FdUrd (410-5M) alone inhibited growth.In the presence of FdUrd (410-5M) thymidine (410-4M) and thymidylate (410-4M) enhanced the specific activity of the DNA up to respectively 68% and 63% of that in the H2O treated controls. Hydroxyurea (4 mg/ml) inhibited DNA-synthesis and growth in the same manner as FdUrd.The prolonged DNA-synthesis and the reversal of the FdUrd effect by thymidine or thymidylate are discussed.
    Planta 05/1974; 118(2):171-181. · 3.38 Impact Factor
  • Ingrid Capesius, Michael Stöhr
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    ABSTRACT: Summary The relative DNA content (arbitrary units) per nucleus was determined in isolated nuclei of the etiolated hypocotyl ofSinapis alba by Feulgen microdensitometry and microfluorometry. An increase of endopolyploidy during elongation growth was detected. With increasing hypocotyl age (40 to 64 hours) the DNA concentration in the chromocenters increases. A hyperreplication of the DNA in the polyploid nuclei can be assumed. Biochemical determinations of DNA and the relative DNA amounts per nucleus determined by cytophotometry agree closely. Bacterial contamination has been excluded to account for the biochemically determined DNA increase.
    Protoplasma 01/1974; 82(1):147-153. · 2.86 Impact Factor
  • Martin Bopp, Ingrid Capesius
    Plant Biology 08/1973; 86(5‐9). · 2.32 Impact Factor
  • Ingrid Capesius
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    ABSTRACT: The enzyme activity of thymidine kinase and thymidylate kinase was studied in seedlings of Sinapis alba during 29 to 90 h after sowing. The maximum of the enzyme activities is reached later than that of DNA synthesis. Addition of FdUrd or thymidine 40 h after sowing results in an increase of the activity of thymidine kinase within the hypocotyls. On the other hand the activity of thymidylate kinase was decreased by addition of FdUrd by 30 to 57%. This inhibition of the enzyme activty could be overcome by simultaneous addition of thymidine. A gradient of the activities of the two enzymes decreasing from the hypocotyl hook to the base was found.
    Zeitschrift für Pflanzenphysiologie. 05/1973; 69(5):438–446.
  • I Capesius, M Bopp
    Hoppe-Seyler's Zeitschrift für physiologische Chemie 06/1972; 353(5):699.
  • I Capesius, M Bopp, W Clauss
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    ABSTRACT: In the seedlings of Sinapis alba, the lag-phase between the application of 5-FUDR and the beginning of the inhibition of elongation growth and the inhibition of DNA-synthesis has been studied. The elongation was retarded after 7 h, and then, depending on the concentration of the FUDR, was completely stopped. In the cotyledons the DNA-synthesis was strongly reduced after about 50 minutes, and in the hypocotyls a lag-phase of less than 30 minutes was observed. With the addition of thymidine the DNA-synthesis was immediately resumed, while the growth began with a lag-phase of 5-7 h. In every case the change in the DNA-synthesis preceded the change in the elongation growth. The inhibition of elongation growth could, therefore, be the consequence of inhibition of DNA-synthesis.
    Planta 03/1972; 103(1):65-73. · 3.38 Impact Factor
  • Martin Bopp, Ingrid Capesius
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    ABSTRACT: DNA-synthesis in the hypocotyls of Sinapis alba L. was studied with H3-thymidine labelling. Cells from hypocotyl segments were stained by the Feulgen-method and squash preparations were made. The following labelling patterns were observed: 1. Labelling of the chromocentres only. 2. Nuclear area evenly labelled. 3. No radioactivity in the chromocentres. This pattern was rarely seen. — The frequency of the first two types in different tissue segments is not equal. In segments with more differentiated cells there was an increase in the percentage of nuclei with radioactivity only in the chromocentres. This could be due to a prolongation of the phase of synthesis in the chromocentres in this tissue. — The total number of labelled nuclei decreases basipetally as well as with the age of the hypocotyl. In hypocotyls of seedlings older than 52 hrs radioactivity appeared only sporadically in the nuclei. The decrease in the number of labelled nuclei is faster than the decline of the corresponding measurable total DNA synthesis in the hypocotyl. This can either be due to extra nuclear DNA synthesis or depend on an increase in DNA synthesis in the later replicating heterochromatic region of the nucleus.
    Chromosoma 11/1971; 33(4):386-395. · 3.34 Impact Factor
  • M Bopp, I Capesius
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    ABSTRACT: Seedlings of Sinapis alba which were grown in light for 24, 48 and 72 h after 36 h of dark treatment renew elongation when transferred to dark again. The rate of elongation decreases with increased light treatment. The per cent as well as absolute inhibition of elongation by FUDR decreases with the duration of light treatment. The shortening of the hypocotyl is due mainly to the inhibition of cell elongation. The inhibition is not directly proportional to DNA synthesis at any particular time.Plants without cotyledons are less inhibited than those with cotyledons. Cytosine arabinoside is inhibitory only at high concentrations. According to these results elongation inhibition by FUDR does not involve the entire DNA-synthesis.
    Planta 03/1971; 96(1):35-42. · 3.38 Impact Factor