Ingrid Capesius

Universität Heidelberg, Heidelburg, Baden-Württemberg, Germany

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Publications (29)51.28 Total impact

  • I. Capesius · S. Loeben ·
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    ABSTRACT: Isolated leaves of Lobularia maritima floating on a 0.2 M NaCl solution for 68 hours were induced to succulence as determined by doubling of leaf thickness and leaf fresh weight due to increases in cell volume of palisade and sponge parenchyma cells. Control leaves floating on water showed a 30% increase in wet weight due to water uptake and only minimal thickening. The absolute DNA content per leaf (but not the DNA content per gram of leaf tissue) increased by 30% over the DNA content of control leaves. This increase is due to the increase of the proportion of individual nuclei with increased nuclear DNA contents as determined by ethidium bromide fluorescence measurements. The DNA increase is mainly due to the selective synthesis of a DNA fraction with high GC content which shows as a separate peak with melting temperature 95.5°C in the thermal denaturation profile in 0.12 M phosphate buffer and a separate band (1.723 g cm-3) in the buoyant density profile in neutral CsCl gradient centrifugation. The high GC fraction can be separated preparatively from main band DNA by Ag+/Cs2SO4 centrifugation.
    Zeitschrift fü Pflanzenphysiologie 05/1983; 110(3):259–266. DOI:10.1016/S0044-328X(83)80108-1
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    ABSTRACT: A satellite DNA with a buoyant density equal to that of main band DNA in neutral cesium chloride (‘cryptic satellite’) can be isolated from the DNA of mustard (Sinapis alba) nuclei by Ag+/Cs2SO4 density gradient centrifugation. This satellite is cleaved into 172 bp repeat units by HinfI, AluI or HaeIII. The HinfI fragments have been further cleaved by AluI, and seven AluI subfragments have been sequenced. As a result two versions of a basic 172 HinfI repeat have been found, one (A + B) with an additional HinfI site. These two sequences (A + B and C) are the most frequent versions of the basic repeat of mustard satellite DNA. The basic 172 bp unit does not contain subrepeats or palindromic sequences. It is not similar (at a criterion of 15 common bases) with any known satellite sequence. It is not unusually highly methylated in the native state.
    Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 04/1983; 739(3):276-280. DOI:10.1016/0167-4781(83)90101-X · 1.70 Impact Factor
  • I. Capesius · H.J. Reiter ·
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    ABSTRACT: Using actinomycin D/CsCl gradient centrifugation two GC-rich satellite fractions comprising 19% of total DNA can be isolated from nuclear DNA of Sinapis alba. The buoyant density is 1.703 g cm-3 for satellite I rich in ribosomal cistrons and 1.705 g cm-3 for satellite II.
    Zeitschrift fü Pflanzenphysiologie 12/1982; 108(5):437–442. DOI:10.1016/S0044-328X(82)80169-4
  • I. Capesius · M. Bopp · K. Nishitani · Y. Masuda ·
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    ABSTRACT: Etiolated seedlings of Sinapis alba treated for 24 hours with 40 µM FdUrd become inhibited in elongation growth.64 hours old seedlings only reach the same length as untreated plants of 48 hours.A comparison of untreated seedlings of 48 and 64 hours with inhibited plants of 64 hours gives the following results: The minimum stress relaxation time is the same for seedlings of the same size, whereas the extensibility is more dependent on the seedling’s age.The total cell wall material content of the treated plants lies between the two controls, but the composition of the wall material (pectin and hemicelluloses) is not significantly changed by the treatment, only arabinose is significantly enhanced.From these results it can be concluded that FdUrd does not inhibit the growth by changing the formation or composition of the cell wall.The reduction of wall material is rather a consequence of the reduced growth.
    Zeitschrift fü Pflanzenphysiologie 08/1981; 103(1):87–93. DOI:10.1016/S0044-328X(81)80244-9
  • I Wagner · I Capesius ·
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    ABSTRACT: The relative amounts of the five nucleosides (deoxycytidine, 5-methyldeoxycytidine, deoxyadenosine, deoxyguanosine and thymidine) in the DNA of nine plant species, one plant satellite DNA, and one animal species were determined by high performance liquid chromatography. The method allows the clean separation of the nucleosides from 10 microgram samples with 15 min. The following values for the proportion of methylated cytosines among all cytosines were obtained: Lobularia maritima 18.5%, Nicotiana tabacum 32.6%, Pisum sativum 23.2%, Rhinanthus minor 29.2%, Sinapsis alba 12.2%, Vicia faba 30.5%, Viscum album 23.2%, Cymbidium pumilum 18.8%, Cymbidium pumilum AT-rich satellite DNA 15.8%, Triticum aestivum 22.4%. DNA of an animal, the gerbil, Meriones unguiculatus, had a methylation percentage of 3.1%. An estimate of the GC content based on the buoyant density of DNA tends to be lower than the actual value, an estimate based on the melting temperature tends to be higher. This supports the finding by other authors that DNA methylation decreases the buoyant density and may increase the melting temperature at high m5C concentration.
    Biochimica et Biophysica Acta 07/1981; 654(1):52-6. DOI:10.1016/0005-2787(81)90135-0 · 4.66 Impact Factor
  • Y. Mohamed · I. Capesius ·
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    ABSTRACT: The dwarf pea variety «Kleine Rheinländerin» responds to GA3 application with increased growth. FdUrd inhibits the elongation growth caused by GA3 when both substances are applied simultaneously and reduces growth below untreated control levels when applied alone. The effect of either substance is most pronounced in the third and forth internode. Both stimulation and inhibition of growth are less pronounced in the lower internodes. FdUrd alone or in combination with GA3 prevent formation of the fifth internode. This effect is due mainly to an inhibition of mitosis in the meristematic tissue. Quantitative and qualitative DNA changes were studied in the same system. GA, application is followed by a pronounced increase in total nuclear DNA per plant; there is reduced DNA synthesis upon FdUrd application. A change in the overall base composition or the appearance of satellite DNA was not observed with either treatment. DNA from all samples had a density of 1.695 g/cm3 corresponding to 35.7 % GC content. The thermal denaturation profile of DNA samples obtained from plants after either treatment was the same with a mean melting temperature of 86.4 ± 0.2 °C. The different GC content suggested by the melting temperature as compared to density determinations is due to a large proportion of 5-methyl cytosine. The reassociation kinetics of thermally denatured DNA was followed after either treatment, and no difference to that of control plant DNA was detected. The results indicate that GA3 stimulates elongation growth and DNA synthesis while FdUrd inhibits both in comparison to untreated control plants. No differential replication or inhibition of any limited DNA fraction was detectable. Both GA3 and FdUrd act by stimulating resp. inhibiting the rate of mitotic activity and endopolyploidization in the shoot.
    Zeitschrift fü Pflanzenphysiologie 06/1980; 98(1):15–23. DOI:10.1016/S0044-328X(80)80215-7
  • Source
    I Capesius · W Krauth · D Werner ·

    FEBS Letters 03/1980; 110(2):184-6. DOI:10.1016/0014-5793(80)80068-8 · 3.17 Impact Factor
  • Ingrid Capesius ·
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    ABSTRACT: The DNA from mustard (Sinapis alba L.) seedlings was examined by neutral CsCl and Ag+/Cs2SO4 density gradient centrifugation. Different satellite fractions were revealed by these two methods. The satellite fractions obtained from the Ag+/Cs2SO4 density gradient could not be generally correlated with satellite DNA fractions observed in CsCl. In CsCl density gradient centrifugation, a main band at density 1,695 g/cm3 and a heavy shoulder at density 1,703 g/cm3 are found. By preparative CsCl gradient centrifugation the heavy shoulder can be enriched but not completely separated from the main band DNA.—Gradient centrifugation by complexing the DNA with Ag+ rf. 0.25 to DNA phosphate reveals three distinct fractions which are further characterized: The heavy satelite DNA fraction revealed by Ag+/Cs2SO4 gradient centrifugation has the same density in a CsCl gradient and the same Tm value as the main band, but differs from main band DNA in the details of its melting profile and in its renaturation kinetics. The light Ag+/Cs2SO4 satellite DNA fraction had a higher melting temperature corresponding to a GC-rich base composition. Differences between these 3 fractions are observed in thermal denaturation and renaturation profiles, hybridization in situ with ribosomal RNA, and their response to restriction endonuclease digestion. The light satellite fraction from the Ag+/Cs2SO4 gradient, rich in ribosomal cistrons corresponds to the heavy shoulder DNA of neutral CsCl gradients which also is rich in ribosomal cistrons. The heavy satellite fraction from Ag+/Cs2SO4 gradient which contains highly repetitive short nucleotide sequences could not be revealed by the classical CsCl gradient centrifugation technique.
    Plant Systematics and Evolution 01/1979; 133(1):1-13. DOI:10.1007/BF00985875 · 1.42 Impact Factor
  • Ingrid Capesius · Walter Nagl ·
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    ABSTRACT: The species- and genus-specific DNA content, average base composition of nuclear DNA, presence or absence of satellite DNA, the percentage of heterochromatin and other characteristics of nuclear DNA and nuclear structure allow to deduce the molecular changes which accompanied, or more probably caused, cladogenesis in the orchids studied. It is suggested that saltatory replication (generative amplification) of certain DNA sequenes, diversification of reiterated DNA sequences, and loss of DNA play an important role in the evolution of orchids.—The relationship between changes of genome composition and of nuclear structure and ultrastructure is discussed on the basis of cot curves, heterochromatin staining with Giemsa (C banding), electron microscopy of nuclei, and molecular hybridization in situ.
    Plant Systematics and Evolution 08/1978; 129(3):143-166. DOI:10.1007/BF00990757 · 1.42 Impact Factor
  • W. Nagl · I. Capesius ·
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    ABSTRACT: Endopolyploidy has been detected in some varieties ofHelianthus annuus L. (Asteraceae/Compositae) by means of scanning photometry of Feulgen-stained nuclei and analysis of nuclear structure. In the hypocotyl cells of seedlings, ploidy levels reach respectively 8 C and 16 C in different varieties, in the root cells 8 C and 16 C; in the cotyledons of ripening seeds 4 C to 8 C values have been found, while all nuclei of the inflorescence axis of one variety exhibit a DNA content of 4 C.—This is the first report of endopolyploidy in a non-succulentAsteraceae species. The characteristic distribution of the endopolyploidy levels in different varieties suggests a strong genetic and/or hormonal control of the final nuclear DNA content in differentiated cells.
    Plant Systematics and Evolution 12/1976; 125(4):261-268. DOI:10.1007/BF00996243 · 1.42 Impact Factor
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    I Capesius ·

    FEBS Letters 11/1976; 68(2):255-8. DOI:10.1016/0014-5793(76)80448-6 · 3.17 Impact Factor
  • W. Nagl · I. Capesius ·
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    ABSTRACT: As a contribution for the study of systematic and evolutionary relationships it is suggested to analyze nuclear DNA and chromatin by means of CsCl ultracentrifugation, thermal denaturation and renaturation, scanning densitometry, and (ultra)structural analyses. Relevant data have been obtained forHelianthus annuus as a first example.The 2C DNA content of four cultivars ofHelianthus annuus L. was calibrated by comparative measurement withAllium cepa nuclei using a scanning densitometer in on-line operation with a computer. Significant infraspecific variation could be detected: cvar. Amerikanische Riesen displayed 6.1 pg, cvar. Gefllte Vielbltige 9.9 pg, cvar. Russian Mammoth 8.9 pg, and a Heidelberg strain 8.7 pg.The buoyant density in neutral CsCl was determined for cvar. Amerikanische Riesen to be 1.695 g cm–3; this corresponds to an average GC content of 35.1%. Thermal denaturation revealed a melting temperature of 86.4C. Derivative thermal denaturation profiles led to the detection of several distinct DNA fractions.The species-specific nuclear structure is of the chromonematic type, but in differentiated cells the chromatin fibers may be more decondensed so that a chromomere-interchromomere structure appears. The heterochromatin constitutes an average of 4.5% of the total genome. Chromatin ultrastructure is characterized by a diffuse distribution of chromatin threads and patches. Nucleosomes of 110 diameter can be recognized.The data are discussed (a) in relation to findings on DNA variation in other plants, (b) in relation to the systematic usefulness and further characterization of nuclear DNA and chromatin, and (c) in relation to tissue-specific and functional variation of the species-specific chromatin structure.
    Plant Systematics and Evolution 05/1976; 126(2):221-237. DOI:10.1007/BF00981672 · 1.42 Impact Factor
  • W. Barthlott · I. Capesius ·
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    ABSTRACT: Zusammenfassung 1. Das Velamen radicum tropischer Orchidaceae aus 17 Gattungen wird raster-elektronenmikroskopisch untersucht; durch Markierung mit tritiiertem Wasser wird die Absorptionsfähigkeit der Luftwurzeln von sieben Gattungen geprüft.2. Die Wände aller Velamenzellen der untersuchten Orchidoideae sind mit regelmäßigen Wandleisten und nativen Poren ausgestattet. Der räumliche Verlauf der Leisten und davon abhängig Verteilung und Größe der Wanddurchbrechungen wird genauer dargestellt und durch REM-Aufnahmen illustriert. Es lassen sich mehrere Variationen der Strukturierungsmuster von Velamenzellen finden, die vermutlich taxonspezifisch sind.3. Die Isotopenmarkierungen ergeben, daß das vom Velamen der Luftwurzeln bei Acampe, Epidendrum, Microcoelia und Rhynchostylis aufgenommene Wasser dem lebenden Gewebe zugeführt wird. Dagegen haben die dünnen, geotrop kaum beeinflußten Luftwurzeln der heterorhizen Gattungen Ansellia, Catasetum und Graphorkis nichts mit der Funktion der Wasseraufnahme zu tun.
    Plant Biology 10/1975; 88(3). DOI:10.1111/j.1438-8677.1975.tb02473.x · 2.63 Impact Factor
  • I Capesius · B Bierweiler · K Bachmann · W Rücker · W Nagl ·
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    ABSTRACT: The DNA of aseptically grown protocorms of a Cymbidium hybrid and in vitro developed leaves, as well as DNA of leaves and flower buds of Cymbidium ceres from the greenhouse, was analysed by analytical ultracentrifugation and thermal denaturation. Upon ultracentrifugation a satellite DNA with a buoyant density of 1.682 g/cm-3 appears as a shoulder on the main band (density 1.694 g/cm-3). Thermal denaturation reveals an inhomogeneous main peak with the major component melting at 84 degrees C and a separate peak melting at 75 degrees C. This is the first demonstration of a satellite DNA in a monocot, and one of the rare examples of a major A + T-rich DNA fraction in a plant.
    Biochimica et Biophysica Acta 07/1975; 395(1):67-73. · 4.66 Impact Factor
  • Ingrid Capesius · Wilhelm Barthlott ·

    Zeitschrift fü Pflanzenphysiologie 07/1975; 75(5):436-448. DOI:10.1016/S0044-328X(75)80138-3
  • I. Capesius · B. Bierweiler · K. Bachmann · W. Rücker · W. Nagl ·
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    ABSTRACT: The DNA of aseptically grown protocorms of a Cymbidium hybrid and in vitro developed leaves, as well as DNA of leaves and flower buds of Cymbidium ceres from the greenhouse, was analysed by analytical ultracentrifugation and thermal denaturation. Upon ultracentrifugation a satellite DNA with a buoyant density of 1.682 g/cm3 appears as a shoulder on the main band (density 1.694 g/cm3). Thermal denaturation reveals an inhomogeneous main peak with the major component melting at 84°; C and a separate peak melting at 75°; C. This is the first demonstration of a satellite DNA in a monocot, and one of the rare examples of a major DNA fraction in a plant.
    Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 06/1975; 395(1):67–73. DOI:10.1016/0005-2787(75)90234-8
  • Ingrid Capesius ·
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    ABSTRACT: Leaves of Lobularia maritima floating on 0,2 M NaCl show a greater increase in fresh weight than leaves on water. The synthesis of nucleic acids — studied by incorporation of 3H-Thymidin and 32P-Phosphate — was lower on NaCl than on H2O. The synthesis of DNA and RNA was strongly inhibited by 8 · 10−5 M FdUrd; during a experimental duration of 21 hours DNA-synthesis was inhibited by 90 %, RNA by 67 %. In 21 hours fresh weight is not influenced by FdUrd. The lag phase of the FdUrd effect on DNA synthesis was about 4–8 hours.
    Zeitschrift fü Pflanzenphysiologie 08/1974; 73(3):266–272. DOI:10.1016/S0044-328X(74)80158-3
  • Wilhelm Barthlott · Ingrid Capesius ·

    Zeitschrift fü Pflanzenphysiologie 06/1974; 72(5):443-455. DOI:10.1016/S0044-328X(74)80065-6
  • I. Capesius · M. Bopp ·
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    ABSTRACT: Etiolated elongating hypocotyls of Sinapis alba synthesize DNA 40–72 h after sowing. The increase in DNA content is about 62.5%. Four-hour pulse experiments showed an increasing rate of [32P]orthophosphate incorporation; the maximum rate was obtained 64–68 h after sowing. In the cotyledons DNA increase up to the 56th h after sowing; later the DNA-content remains constant. During this time the incorporation of 32P into the DNA of the cotyledons decreases continuously.After simultaneous application of Fluordesoxyuridin (FdUrd) (410-5M) and thymidine (410-4M) or thymidylate (410-4M) 40–64 h after sowing, the growth rate was the same as that in H2O treated controls, whereas application of FdUrd (410-5M) alone inhibited growth.In the presence of FdUrd (410-5M) thymidine (410-4M) and thymidylate (410-4M) enhanced the specific activity of the DNA up to respectively 68% and 63% of that in the H2O treated controls. Hydroxyurea (4 mg/ml) inhibited DNA-synthesis and growth in the same manner as FdUrd.The prolonged DNA-synthesis and the reversal of the FdUrd effect by thymidine or thymidylate are discussed.
    Planta 05/1974; 118(2):171-181. DOI:10.1007/BF00388392 · 3.26 Impact Factor
  • Ingrid Capesius · Michael Stöhr ·
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    ABSTRACT: Summary The relative DNA content (arbitrary units) per nucleus was determined in isolated nuclei of the etiolated hypocotyl ofSinapis alba by Feulgen microdensitometry and microfluorometry. An increase of endopolyploidy during elongation growth was detected. With increasing hypocotyl age (40 to 64 hours) the DNA concentration in the chromocenters increases. A hyperreplication of the DNA in the polyploid nuclei can be assumed. Biochemical determinations of DNA and the relative DNA amounts per nucleus determined by cytophotometry agree closely. Bacterial contamination has been excluded to account for the biochemically determined DNA increase.
    Protoplasma 03/1974; 82(1):147-153. DOI:10.1007/BF01276877 · 2.65 Impact Factor