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ABSTRACT: To analyze the diversity of F1 in laboratory line Oncomelania hupensis hupensis.
Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes were amplified by polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed by GENETYX-MAC software package (ver. 9).
Pairwise divergences among six F1 individuals were found in 12.2% nucleotides in COI gene fragments. 13.5% of genetic divergences between O. h. hupensis and O. h. robertsoni were identified. 94 amino acids were observed in difference. In Cytb gene fragments, 6.4% of divergence was observed among four F1 samples. 25 amino acids were observed differently. The divergence of Cytb genes from O. h. robertsoni and O. h. hupensis were 13.6%, included 6 amino acids.
Diversities were found in both COI and Cytb gene sequences of F1 in laboratory line O. h. hupensis.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 11/2004; 22(5):274-6.