Publications (14)31.7 Total impact
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Article: Norditerpenoid and dianthramide glucoside alkaloids from cultivatedaconitum species from Korea
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ABSTRACT: A new norditerpene alkaloid named 8-O-methylhypaconine (1) was isolated along with twelve known alkaloids from the underground parts of an unknown species ofAconitum plant culti-vated in Korea. Among the known alkaloids, two dianthramide glucosides, N-(2′-β-glucopyra-nosyl-5′-hydroxysalicyl)-5-hydroxyanthranilic acid methyl ester (2) and N-(2′-β-glucopyranosyl-5′-hydroxysalicyl)-5-hydroxy-6-methoxyanthranilic acid methyl ester (3), were isolated fromAconitum plants for the first time. The structures were established on the basis of chemical and spectroscopic methods.Archives of Pharmacal Research 04/2012; 30(6):691-694. · 1.59 Impact Factor -
Article: Hepatoprotective and Antioxidative Activities of Cornus officinalis against Acetaminophen-Induced Hepatotoxicity in Mice.
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ABSTRACT: The fruit of Cornus officinalis Sieb. et Zucc. is commonly prescribed in Asian countries as a tonic formula. In this study, the hepatoprotective effect of ethanolic extracts of the fruit of C. officinalis (ECO) was investigated in a mouse model of acetaminophen- (APAP-) induced liver injury. Pretreatment of mice with ECO (100, 250, and 500 mg/kg for 7 days) significantly prevented the APAP (200 mg/kg) induced hepatic damage as indicated by the serum marker enzymes (AST, ALT, and LDH). Parallel to these changes, ECO treatment also prevented APAP-induced oxidative stress in the mice liver by inhibiting lipid peroxidation (MDA) and restoring the levels of antioxidant enzymes (SOD, CAT, and HO-1) and glutathione. Liver injury and collagen accumulation were assessed using histological studies by hematoxylin and eosin staining. Our results indicate that ECO can prevent hepatic injuries associated with APAP-induced hepatotoxicity by preventing or alleviating oxidative stress.Evidence-based Complementary and Alternative Medicine 01/2012; 2012:804924. · 4.77 Impact Factor -
Article: Genotoxicity assessment of a herbal formula, Ojeok-san.
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ABSTRACT: Ojeok-san (OJS, wuji powder, goshaku-san), a widely used herbal formula in traditional Korean medicine, is used to treat illnesses such as the common cold, fatigue and gastrointestinal disorders; however there is insufficient background information about its safety. To establish safety information for OJS, we evaluated its genotoxicity. The ability of OJS to induce reverse mutations was evaluated in Salmonella typhimurium (TA100, TA1535, TA98 and TA1537) and Escherichia coli (WP2uvrA) in the presence or absence of the metabolic activation system (S-9 mix). Chromosomal aberrations were evaluated in response to OJS, and viability and metaphase were analyzed in Chinese hamster lung (CHL) cells in the presence or absence of S-9 mix. A micronucleus test was performed using bone marrow cells from male ICR mice. OJS was orally administered twice at a 24h interval at a dose of 500, 1000 and 2000 mg/kg in mice. There were no increases in the number of revertant colonies at any concentrations of OJS regardless of S-9 mix in all tester strains compared to the vehicle control. OJS did not significantly increase the number of structural aberration in CHL cells in the presence or absence of S-9 mix. The oral administration of OJS at doses up to 2000 mg/kg caused no significant increase in the number of micronucleated polychromatic erythrocytes (MNPCEs) and in the mean value for the ratio of PCE to total erythrocytes (PCE/(PCE+NCE)). NCE is normochromatic erythrocyte. OJS did not increase the incidence of MNPCEs in bone marrow. These results suggest that OJS is toxicologically safe on genotoxicity studies.Journal of ethnopharmacology 03/2011; 135(2):586-9. · 2.32 Impact Factor -
Article: Simultaneous determination of liquiritin, hesperidin, and glycyrrhizin by HPLC-photodiode array detection and the anti-inflammatory effect of Pyungwi-san.
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ABSTRACT: A high-performance liquid chromatographic method was developed and validated to determine liquiritin, hesperidin, and glycyrrhizin levels in a traditional Korean medicine, Pyungwi-san (PWS). Reverse-phase chromatography using a C18 column operating at 40oC, and photodiode array detection at 254 nm and 280 nm, were used for quantification of the three marker components of PWS. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Calibration curves were acquired with r (2) > 0.9999, and the relative standard deviation values (%) for intra- and inter-day precision were both less than 4.0%. The recovery of each compound was in the range 97.33-110.72%, with an relative standard deviation less than 6.0%. To provide information on the biological activity of PWS, anti-inflammatory action was evaluated. Production of nitric oxide and prostaglandin E(2) were measured using the Griess reagent and enzyme-linked immunosorbent assay, respectively. PWS showed inhibitory effect on prostaglandin E(2) production in LPS-treated RAW 264.7 cells.Archives of Pharmacal Research 02/2011; 34(2):203-10. · 1.59 Impact Factor -
Article: Safety Evaluation of Yukmijihwang-tang: Assessment of Acute and Subchronic Toxicity in Rats.
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ABSTRACT: Yukmijihwang-tang (YMJ; Liu wei di huang tang (China), Rokumigan (Japan)) has been used in the treatment of diseases including renal disorder, cognitive vitality, and diabetes mellitus. However, there is very little information regarding the toxicity of YMJ to give an assurance of safety for clinical treatment. To provide safety information for YMJ, we evaluated its acute and sub-chronic toxicity in rats. The single-dose toxicity of YMJ was examined using Sprague-Dawley rats. Rats were treated with YMJ extract orally at 0, 500, 1000, or 2000 mg/kg body weight. After a single administration, clinical signs were observed every day for two weeks, and body weights were measured five times, including an initial measurement on day 1 (the day of administration). In the sub-chronic oral toxicity study, YMJ was administered to rats at 0, 500, 1000, or 2000 mg/kg/day for 13 weeks. Mortalities, clinical signs, body weight changes, food and water consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights, and histological examination were monitored during the study period. We found no mortality and no abnormalities in clinical signs, body weights, and necropsy findings for any of the animals in the acute and sub-chronic studies following oral administration in the rat at up to 2000 mg/kg/day YMJ. YMJ may not have any single-dose toxicity; the LD(50) of YMJ was over 2000 mg/kg, and it is safe for rats. The no-observed-adverse-effect-level (NOAEL) was considered to be 2000 mg/kg/day.Evidence-based Complementary and Alternative Medicine 01/2011; 2011:672136. · 4.77 Impact Factor -
Article: Evaluation of genotoxicity of Yukmijihwang-tang, a herbal formula.
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ABSTRACT: Yukmijihwang-tang (Liu wei di huang tang, Rokumigan; YMJ) has been used for body enrichment; however, little toxicological evaluation of YMJ has been performed to assure its safety for clinical treatment. To increase the safety information for YMJ, its genotoxicity was evaluated. There was no increase in the number of revertant colonies in four strains of Salmonella typhimurium or one strain of Escherichia coli at any concentration of YMJ studied, regardless of the including when dosed with YMJ metabolized with and S-9 microsomal fraction. YMJ significantly increased structural aberrations in Chinese hamster lung (CHL) cells at the high concentrations (2500 and 5000 μg/ml) in the presence or absence of metabolic activation by the S-9 microsomal fraction. Oral administration of YMJ at doses up to 2000 mg/kg did not increase the incidence of micronucleated polychromatic erythrocytes in bone marrow. These results suggest that YMJ is not genotoxic at the proper dose.Regulatory Toxicology and Pharmacology 01/2011; 59(3):391-6. · 2.43 Impact Factor -
Article: Evaluation of safety of the herbal formula Ojeok-san: acute and sub-chronic toxicity studies in rats.
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ABSTRACT: Ojeok-san (OJS; wuji powder in China and goshaku-san in Japan), a widely used herbal formula in traditional Korean medicine and Japanese herbal medicine (Kampo medicine), has been used to treat common cold and illnesses including fatigue and gastrointestinal disorders, but there is very little information on its safety. To provide information on the safety of OJS, we evaluated its acute and sub-chronic toxicity in rats. The single and sub-chronic toxicity of OJS was examined using male and female Sprague-Dawley rats. The rats were treated with the OJS extract orally at the highest dose level of 2000 mg/kg/day body weight. After single administration, signs of toxicity were observed every hour for the first 6h and every day for two weeks. In the sub-chronic toxicity study, OJS was administered for 13 weeks. Mortality, clinical signs, body weight changes, food and water consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers were monitored during the study period. We found no mortality and no abnormality in clinical signs, body weight, and necropsy findings for any of the animals in the acute and sub-chronic toxicity study following oral administration of OJS. OJS may not have any single dose toxicity. The lethal dose with a 50% mortality rate (LD(50)) was over 2000 mg/kg. The no-observed adverse effects level (NOAEL) was considered to be 2000 and 1000 mg/kg/day for male and female rats, respectively.Journal of ethnopharmacology 09/2010; 131(2):410-6. · 2.32 Impact Factor -
Article: Formononetin prevents ovariectomy-induced bone loss in rats.
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ABSTRACT: The major risk factor of postmenopausal osteoporosis is estrogen deficiency. Hormone replacement therapy is efficacious against osteoporosis, but it induces several significant adverse effects. In this study, therefore, we compared therapeutic potencies of three phytoestrogens: genistein, daidzein, and formononetin. Our result showed that in Saos-2 cells, formononetin and genistein (5 x 10(-7) M) treatment increased alkaline phosphatase activity by 33.0 +/- 5.8% and 21.1 +/- 4.0%. Genistein inhibited osteoclast formation in a dose-dependent manner. In OVX rats, formononetin-treated groups given 1 and 10 mg/kg/day displayed increased trabecular bone areas (TBAs) within the tibia. Genistein- and daidzein-treated groups also displayed increased tibial TBAs. TBAs of the lumbar vertebrae were higher in all treated groups than in the control group. In conclusion, formononetin as well as other isoflavones, such as daidzein and genistein, inhibited bone loss caused by estrogen-deficiency.Archives of Pharmacal Research 04/2010; 33(4):625-32. · 1.59 Impact Factor -
Article: Inhibitory effects of heartwood extracts of Broussonetia kazinoki Sieb on the development of atopic dermatitis in NC/Nga mice.
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ABSTRACT: We investigated the effects of a topically applied extract of the heartwood of Broussonetia kazinoki Sieb (B. kazinoki) on atopic dermatitis (AD)-like skin lesions induced by an extract of the house-dust mite Dermatophagoides farina in NC/Nga mice. We found that topically applied B. kazinoki extract suppressed the histological manifestations of AD-like skin lesions, and decreased the levels of plasma immunoglobulin E (IgE) and interleukin-4 (IL-4) in the mice. Moreover, B. kazinoki inhibited the induction of thymus-and-activation-regulated chemokine (TARC/CCL17), macrophage-derived chemokine (MDC/CCL22), and regulated-on-activation-normal T cell-expressed-and-secreted chemokine (RANTES/CCL5) in HaCaT cells activated by tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). In conclusion, our results suggest that B. kazinoki extract has therapeutic advantages in the treatment of AD.Bioscience Biotechnology and Biochemistry 01/2010; 74(9):1802-6. · 1.28 Impact Factor -
Article: Rat growth-hormone release stimulators from fenugreek seeds.
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ABSTRACT: Bioassay-guided fractionation of MeOH extract from fenugreek (Trigonella foenum-graecum L.) seeds resulted in the isolation of two rat growth-hormone release stimulators in vitro, fenugreek saponin I (1) and dioscin (9), along with two new, i.e., 2 and 3, and five known analogues, i.e., 4-8. The structures of the new steroidal saponins, fenugreek saponins I, II, and III (1-3, resp.), were determined as gitogenin 3-O-beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranoside, sarsasapogenin 3-O-beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranoside, and gitogenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside, respectively. Fenugreek saponin I (1) and dioscin (9) caused ca. 12.5- and 17.7-fold stimulation of release, respectively, of rat growth hormone from rat pituitary cells, whereas gitogenin (5) showed moderate activity. To our knowledge, this is the first study to demonstrate that steroidal saponins stimulate rat growth-hormone release in rat pituitary cells.Chemistry & Biodiversity 10/2008; 5(9):1753-61. · 1.80 Impact Factor -
Article: Triterpenoid saponins from the seeds of Pharbitis nil.
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ABSTRACT: From the seeds of Pharbitis nil (Convolvulaceae), two new oleanene-type triterpene glycosides, pharbitosides A (1) and B (2), together with beta-sitosterol, beta-sitosterol glucoside (daucosterol), caffeic acid, and methyl caffeate were isolated. The structure of pharbitoside A (1) was elucidated to be queretaroic acid 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside (1). Pharbitoside B (2) is a 21alpha-hydroxyoleanolic acid saponin carrying the same sugar moiety as that of pharbitoside A (1).CHEMICAL & PHARMACEUTICAL BULLETIN 03/2008; 56(2):203-6. · 1.59 Impact Factor -
Article: Induction of growth hormone release by dioscin from Dioscorea batatas DECNE.
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ABSTRACT: In this study, dioscin was isolated from Dioscoreae Rhizoma (DR), which is the rhizome of Dioscorea batatas D(ECNE). that inhabits broad areas of Korea and Japan. To determine whether dioscin induced growth hormone (GH) release, we evaluated its induction effects on GH release both in vitro and in vivo. The 70% methanol extract of DR, and its n-hexane and n-BuOH fractions, induced rat GH (rGH) release in rat pituitary cells 10-fold, 8-fold, and 5- fold higher than the control (0.36 +/- 0.02 nM), respectively (p < 0.05 each). The dioscin-induced rGH release of the cells was concentration-dependent and its ED(50) was 1.14 x 10(-5) M. Within 90 minutes after intravenous administration of 10 microg/kg (p < 0.05 at t(max)), dioscin caused the greatest increase in rGH concentration (C(max)) in the rat plasma (34.16 +/- 14.10 ng/ml) (n = 4), which was twice as high as the control group (12.88 +/- 3.29 ng/ml) (n = 27).Journal of biochemistry and molecular biology 12/2007; 40(6):1016-20. · 2.02 Impact Factor -
Article: Induction of growth hormone release by glycyrrhizae radix on rat.
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ABSTRACT: Induction of growth hormone (GH) by Glycyrrhizae Radix (GR), one of the most popular herbal medicine, and its major ingredients were studied in rat pituitary cells in vitro and in vivo assay. The MeOH extract and the n-hexane (HX) fraction of GR induced rat GH (rGH) release up to 1.89 times (0.34 +/- 0.04 nM) and 4.59 times (0.83 +/- 0.03 nM), compared to the basal level (p < 0.05). Among many ingredients isolated and purified from GR both glycyrrhetinic acid and glycyrrhizin induced significantly rGH release compared to the control (p < 0.05). After an intravenous injection of rat growth hormone releasing hormone (rGHRH) (10 microg/kg) as positive control, in SD rats, Tmax of plasma rGH level was 10 min, C(max) was 3.84 +/- 0.01 nM (n = 3), and enhanced plasma rGH level returned to the baseline in 90 min. Both AUC(0-90) (area under the curve) of plasma rGH level after HX fraction and that after rGHRH administration were increased significantly from the basal level, respectively (p < 0.01). In conclusions, HX fraction is the most active fraction of MeOH extract of GR in rGH induction.Journal of biochemistry and molecular biology 12/2007; 40(6):979-85. · 2.02 Impact Factor -
Article: Norditerpenoid and dianthramide glucoside alkaloids from cultivated Aconitum species from korea.
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ABSTRACT: A new norditerpene alkaloid named 8-O-methylhypaconine (1) was isolated along with twelve known alkaloids from the underground parts of an unknown species of Aconitum plant culti vated in Korea. Among the known alkaloids, two dianthramide glucosides, N-(2'-beta-glucopyra nosyl-5'-hydroxysalicyl)-5-hydroxyanthranilic acid methyl ester (2) and N-(2'-beta-glucopyranosyl-5'-hydroxysalicyl)-5-hydroxy-6-methoxyanthranilic acid methyl ester (3), were isolated from Aconitum plants for the first time. The structures were established on the basis of chemical and spectroscopic methods.Archives of Pharmacal Research 07/2007; 30(6):691-4. · 1.59 Impact Factor
Top co-authors
Top Journals
Institutions
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2012
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Kyung Hee University
Seoul, Seoul, South Korea
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2007–2010
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Korea Institute of Oriental Medicine
Bucheon, Gyeonggi, South Korea -
Seoul National University
Seoul, Seoul, South Korea
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