-
[show abstract]
[hide abstract]
ABSTRACT: SummaryAssociation between SNPs in polymorphism in proliferator-activated receptor-gamma (PPARG) and peak bone mineral density (BMD) variation of women was measured in 401 Chinese nuclear families using quantitative transmission
disequilibrium test (QTDT). The peak BMD variation was not attributable to PPARG in our sample.
Introduction The purpose of this study is to test whether genetic PPARG might play a role in normal variation in peak BMD.
Methods We genotyped 10 tagging SNPs in PPARG using allele-specific polymerase chain reaction and further test whether these SNPs were associated with peak BMD variation
at the lumbar spine and femoral neck of women in 401 Chinese nuclear families using QTDT. Furthermore, the association between
these SNPs in PPARG and BMD in 710 postmenopausal Chinese women was measured.
ResultsUsing QTDT for within-family association, we failed to find that single SNP and haplotype were significantly associated with
peak BMD at the lumbar spine and femoral neck. Meanwhile, we found that only rs1801282 was significantly associated with BMD
at the lumbar spine in postmenopausal women (P = 0.013).
ConclusionsOur present results suggest, for the first time, that the genetic polymorphism in PPARG is not a major contributor to the observed variability in peak BMD at the lumbar spine and femoral neck in Chinese women.
KeywordsBone mineral density-Polymorphism-Proliferator-activated receptor-gamma-Transmission disequilibrium test
Osteoporosis International 04/2012; 21(5):873-882. · 4.58 Impact Factor
-
Animal Genetics 04/2012; 43(2):237-8. · 2.40 Impact Factor
-
W-J Xiao,
Y-H Ke,
J-W He,
H Zhang,
J-B Yu,
W-W Hu,
J-M Gu,
G Gao,
H Yue,
C Wang,
Y-Q Hu,
M Li,
Y-J Liu,
W-Z Fu, Z-L Zhang
[show abstract]
[hide abstract]
ABSTRACT: Association between ten single-nucleotide polymorphisms (SNPs) in the human ALOX12 and ALOX15 genes and variations in peak bone mineral density (BMD) in a large sample of Chinese nuclear families with female offspring using the quantitative transmission disequilibrium test (QTDT). Our results suggest that the genetic polymorphisms in both human ALOX12 and ALOX15 may contribute to variations in the peak BMD of Chinese women.
The aim of this study was to investigate whether polymorphisms in the human ALOX12 and ALOX15 genes are associated with variations in peak BMD in Chinese nuclear families with female offspring.
Each five SNPs in the ALOX12 and ALOX15 genes were genotyped in a total of 1,260 individuals from 401 Chinese nuclear families. The BMD of the lumbar spine, femoral neck and total hip was measured by dual-energy X-ray absorptiometry. We tested whether a single SNP or a haplotype was associated with peak BMD variations using the QTDT.
Using QTDT to measure within-family associations in ALOX15, we observed a significant association between rs916055 and BMD in the lumbar spine (p = 0.027 in the permutation 1,000 test). However, in ALOX12, rs312470 was significantly associated with BMD in the femoral neck (p = 0.029 and p = 0.036 in the permutation 1,000 test). The results of a haplotype analysis supported the findings of the single locus test for ALOX15.
Our results suggest that the genetic polymorphisms in both human ALOX12 and ALOX15 may contribute to variations in the peak BMD of Chinese women.
Osteoporosis International 11/2011; 23(7):1889-97. · 4.58 Impact Factor
-
W-J Xiao,
J-W He, H Zhang,
W-W Hu,
J-M Gu,
H Yue,
G Gao,
J-B Yu,
C Wang,
Y-H Ke,
W-Z Fu,
Z-L Zhang
[show abstract]
[hide abstract]
ABSTRACT: Arachidonate 12-lipoxygenase (ALOX12) is a member of the lipoxygenase superfamily, which catalyzes the incorporation of molecular oxygen into polyunsaturated fatty acids. The products of ALOX12 reactions serve as endogenous ligands for peroxisome proliferator-activated receptor γ (PPARG). The activation of the PPARG pathway in marrow-derived mesenchymal progenitors stimulates adipogenesis and inhibits osteoblastogenesis. Our objective was to determine whether polymorphisms in the ALOX12 gene were associated with variations in peak bone mineral density (BMD) and obesity phenotypes in young Chinese men.
All six tagging single-nucleotide polymorphisms (SNPs) in the ALOX12 gene were genotyped in a total of 1215 subjects from 400 Chinese nuclear families by allele-specific polymerase chain reaction. The BMD at the lumbar spine and hip, total fat mass (TFM) and total lean mass (TLM) were measured using dual-energy X-ray absorptiometry. The pairwise linkage disequilibrium among SNPs was measured, and the haplotype blocks were inferred. Both the individual SNP markers and the haplotypes were tested for an association with the peak BMD, body mass index, TFM, TLM and percentage fat mass (PFM) using the quantitative transmission disequilibrium test (QTDT).
Using the QTDT, significant within-family association was found between the rs2073438 polymorphism in the ALOX12 gene and the TFM and PFM (P=0.007 and 0.012, respectively). Haplotype analyses were combined with our individual SNP results and remained significant even after correction for multiple testing. However, we failed to find significant within-family associations between ALOX12 SNPs and the BMD at any bone site in young Chinese men.
Our present results suggest that the rs2073438 polymorphism of ALOX12 contributes to the variation of obesity phenotypes in young Chinese men, although we failed to replicate the association with the peak BMD variation in this sample. Further independent studies are needed to confirm our findings.
International journal of obesity (2005) 03/2011; 35(3):378-86. · 4.34 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Hip dual-energy X-ray absorptiometry (DXA) images were used to calculate hip bone mineral density (BMD) and hip geometry parameters of 18,502 healthy Chinese people (14,435 women and 4,067 men), 254 subjects sustained a femoral neck fracture and 254 age- and sex-matched controls. Our study showed that thinning of the cortical shell and deterioration of the strength index (SI) in femoral neck with aging in both Chinese men and women. SI may be a risk factor for hip fracture in Chinese women.
The purpose of this study was to evaluate age-related trends in the hip geometry of healthy Chinese men and women and to examine whether changes in hip geometric parameters is one of the risk factors of hip fracture.
We recruited 14,435 women and 4,067 men as the study population. There were 254 subjects (216 women, 38 men) who had sustained a femoral neck fracture; 254 age- and sex-matched healthy persons served as controls. Hip DXA images were used to calculate hip BMD and hip geometry parameters, including the hip axis length (HAL), cross-sectional moment of inertia (CSMI), cross-sectional area (CSA), neck-shaft angle (NSA) and femoral SI.
Hip BMD, CSMI, CSA and SI showed significantly negative correlations with age. However, after adjustment for height and weight, HAL increased with age, and there was no strong correlation between CSMI and age in either sex. In both genders, hip BMD and CSA were significant lower in fracture cases compared with controls. After adjustment for hip BMD, in women only smaller SI (odds ratio [OR] 1.53; 95% confidence interval [CI], 1.04-2.26) was predictive of hip fracture but in men, none of the geometry parameters was associated with hip fracture risk.
This study demonstrated thinning of the cortical shell and deterioration of the resistance to bending and SI with aging in femoral neck in Chinese men and women. SI may be a risk factor for hip fracture that is independent of BMD measurement in Chinese women.
Osteoporosis International 01/2011; 22(9):2513-22. · 4.58 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: IPsec has become a very popular Internet security infrastructure today. As a new key exchange protocol of IPsec, to some extent, IKEv2 can use cookie negotiation mechanism to detect and resist memory-based denial-of-service (DoS) attack in the application layer. However, IKEv2 still cannot avoid IP fragment-based DoS attacks since the IKEv2 messages transmission runs over UDP and there are large IKE messages needed to be fragmented during the exchange process between two IKE peers. In this paper we first investigate some typical methods and give the analysis of their inability against the IP fragmentation DoS attack. To overcome this problem, we design a new IKEv2 header format called M-ISAKMP, and add a new type of Notification Payload and other related strategies. With the novel application-based fragmentation mechanism, our proposed enhanced IKEv2 protocol achieves defending against DoS attack successfully and efficiently.
The 3rd IEEE International Conference on Broadband Network& Multimedia Technology, Beijing, China; 10/2010
-
Osteoporosis International 03/2010; · 4.58 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Association between SNPs in polymorphism in peroxisome [corrected] proliferator-activated receptor-gamma (PPARG) and peak bone mineral density (BMD) variation of women was measured in 401 Chinese nuclear families using quantitative transmission disequilibrium test (QTDT). The peak BMD variation was not attributable to PPARG in our sample.
The purpose of this study is to test whether genetic PPARG might play a role in normal variation in peak BMD.
We genotyped 10 tagging SNPs in PPARG using allele-specific polymerase chain reaction and further test whether these SNPs were associated with peak BMD variation at the lumbar spine and femoral neck of women in 401 Chinese nuclear families using QTDT. Furthermore, the association between these SNPs in PPARG and BMD in 710 postmenopausal Chinese women was measured.
Using QTDT for within-family association, we failed to find that single SNP and haplotype were significantly associated with peak BMD at the lumbar spine and femoral neck. Meanwhile, we found that only rs1801282 was significantly associated with BMD at the lumbar spine in postmenopausal women (P = 0.013).
Our present results suggest, for the first time, that the genetic polymorphism in PPARG is not a major contributor to the observed variability in peak BMD at the lumbar spine and femoral neck in Chinese women.
Osteoporosis International 08/2009; 21(5):873-82. · 4.58 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: We identified 17 polymorphisms in myostatin by sequencing, and three informative single nucleotide polymorphisms (SNPs) were selected for further observation for their association with peak BMD of women in 401 Chinese nuclear families. Our results suggest that genetic polymorphisms in myostatin likely play a role in attainment of peak BMD in Chinese women.
Myostatin is a TGF-beta family member that is a negative regulator of skeletal muscle growth.
We identified SNPs in myostatin by direct sequencing. Furthermore, using a quantitative transmission disequilibrium test (QTDT). we tested and further test whether SNPs were associated with peak bone mineral density (BMD) variation at the spines and hips of 401 Chinese nuclear families. We identified 17 polymorphisms in myostatin by sequencing. Next, we selected three informative SNPs for further observation of an association with peak BMD of premenopausal women in 401 Chinese nuclear families.
Using QTDT for the within-family association, we found significant association between rs2293284 and total hip, femoral neck, and trochanter BMD (all p < 0.05), while rs7570532 was associated with total hip and trochanter BMD (p = 0.034 and p = 0.035, respectively). The within-family association was significant between BMI and +2278G > A (p = 0.022). Subsequent permutations were in agreement with these significant within-family association results. Moreover, analyses of the haplotypes confer further evidence for association of rs2293284 and rs7570532 with hip peak BMD variation.
These results suggest, for the first time, the genetic polymorphisms in myostatin likely play a role in attainment of peak BMD in Chinese women.
Osteoporosis International 01/2008; 19(1):39-47. · 4.58 Impact Factor
