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ABSTRACT: A novel endophytic actinobacterium, designated strain YIM 68236T, was isolated from healthy leaves of Camptotheca acuminata Decne. and characterized by using a polyphasic approach. Cells of this strain occurred singly, in pairs or in tetrads. It grew at 10-45 °C, at pH 5.0-8.0 (optimum pH 7.0) and in the presence of 0-3% (w/v) NaCl. The DNA G+C content was 71.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YIM 68236T belongs to the genus Blastococcus. However, it differed from its closest relatives, Blastococcus aggregatus DSM 4725T, Blastococcus saxobsidens DSM 44509T and Blastococcus jejuensis DSM 19597T in many phenotypic characteristics. Moreover, the levels of DNA-DNA relatedness between the new isolate and the three above-mentioned type strains were 49.0±1.6%, 46.1±3.2% and 39.8±1.5%, respectively. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain YIM 68236T represents a novel species of the genus Blastococcus, Blastococcus endophyticus sp. nov., with strain YIM 68236T (=CCTCC AA 209045T= DSM 45413T=KCTC 19998T) as the type strain.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2013; · 2.11 Impact Factor
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ABSTRACT: The genus Nocardiopsis, a widespread group in phylum Actinobacteria, has received much attention owing to its ecological versatility, pathogenicity, and ability to produce a rich array of bioactive metabolites. Its high environmental adaptability might be attributable to its genome dynamics, which can be estimated through comparative genomic analysis targeting microorganisms with close phylogenetic relationships but different phenotypes. To shed light on speciation, gene content evolution, and environmental adaptation in these unique actinobacteria, we sequenced draft genomes for 16 representative species of the genus and compared them with that of the type species N. dassonvillei subsp. dassonvillei DSM 43111(T). The core genome of 1,993 orthologous and paralogous gene clusters was identified, and the pan-genomic reservoir was found not only to accommodate more than 22,000 genes, but also to be open. The top ten paralogous genes in terms of copy number could be referred to three functional categories: transcription regulators, transporters, and synthases related to bioactive metabolites. Based on phylogenomic reconstruction, we inferred past evolutionary events, such as gene gains and losses, and identified a list of clade-specific genes implicated in environmental adaptation. These results provided insights into the genetic causes of environmental adaptability in this cosmopolitan actinobacterial group and the contributions made by its inherent features, including genome dynamics and the constituents of core and accessory proteins.
PLoS ONE 01/2013; 8(4):e61528. · 4.09 Impact Factor
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ABSTRACT: A novel actinomycete, designated strain KLBMP 1111(T), was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111(T) formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828(T) (98.8%), K. aridum subsp. largum DSM 44150(T) (98.1%) and Kibdelosporangium philippinense DSM 44226(T) (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H(4)); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and an unknown phospholipid; iso-C(16:0), C(16:0), anteiso-C(15:0) and iso-C(15:0) as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA-DNA hybridization values between strain KLBMP 1111(T) and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP 1111(T) represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111(T) (=KCTC 19775(T) = CCTCC AA 2010001(T)).
Antonie van Leeuwenhoek 02/2012; 101(2):433-41. · 2.09 Impact Factor
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ABSTRACT: A Gram-stain-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161(T), was isolated from brine of a salt well in Yunnan province, China, and subjected to a taxonomic study using a polyphasic approach. Cells of strain YIM 95161(T) were short rods, approximately 0.9-1.4 µm long and 0.4-0.6 µm wide. Strain YIM 95161(T) grew at 15-40 °C (optimum, 25-30 °C), 6-29 % (w/v) NaCl (optimum, 14-19 %) and at pH 5.0-8.0 (optimum, pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were summed feature 8 (C(18 : 1)ω6c and/or C(18 : 1)ω7c) and C(14 : 0). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown phosphoglycolipid and two unknown lipids. The DNA G+C content was 69.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 95161(T) was a member of the genus Salinisphaera and exhibited sequence similarities of 96.7 %, 95.6 % and 95.4 % to Salinisphaera shabanensis E1L3A(T), Salinisphaera dokdonensis CL-ES53(T) and Salinisphaera hydrothermalis EPR70(T), respectively. On the basis of phylogenetic, physiological and chemotaxonomic analysis, strain YIM 95161(T) represents a novel species of the genus Salinisphaera, for which the name Salinisphaera halophila sp. nov. is proposed. The type strain is YIM 95161(T) ( = CCTCC AB 2011132(T) = JCM 17431(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 9):2174-9. · 2.11 Impact Factor
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ABSTRACT: A novel Gram-stain-positive, slightly halophilic, catalase-positive, oxidase-negative, endospore-forming, motile, facultatively anaerobic, rod-shaped bacterium, designated strain JSM 081004(T), was isolated from non-saline forest soil in Xiaoxi National Natural Reserve, China. Growth occurred with 0.5-20 % (w/v) NaCl (optimum 2-4 %), at pH 6.0-10.5 (optimum pH 8.0) and at 5-40 °C (optimum 25-30 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C₁₅:₀ and anteiso-C₁₅:₀. Strain JSM 081004(T) contained MK-7 as the predominant respiratory quinone, and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G+C content of strain JSM 081004(T) was 40.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 081004(T) should be assigned to the genus Bacillus and was most closely related to the type strains of Bacillus lehensis (sequence similarity 97.8 %), Bacillus oshimensis (97.8 %) and Bacillus patagoniensis (97.3 %). Phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data all support the proposal of strain JSM 081004(T) as a representative of a novel species of the genus Bacillus, for which the name Bacillus xiaoxiensis sp. nov. is proposed; the type strain is JSM 081004(T) ( = CCTCC AA 208057(T) = DSM 21943(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2011; 61(Pt 9):2095-100. · 2.11 Impact Factor
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ABSTRACT: Three actinomycetal strains, designated YIM 90479, YIM 90480(T) and YIM 90600(T), were isolated from a salt field in Xinjiang province, North-west China and subjected to polyphasic taxonomic study. All three strains were moderately halophilic and were able to grow on agar at NaCl concentrations of up to 20-25 % (w/v). No growth was observed in the absence of NaCl. Good growth occurred at 37 °C and 15 % (w/v) NaCl. The cell walls of the three strains were of the type IV variety and their phospholipid patterns were type PIII. MK-9 (H(4)) and MK-10 (H(4)) or MK-9 (H(4)) and MK-9 (H(2)) were the predominant menaquinones. iso-C(16 : 0) and anteiso-C(17 : 0) or anteiso-C(15 : 0) and anteiso-C(17 : 0) were the major fatty acids. The G+C contents of the genomic DNA were 66.4-68.3 mol%. Based on differential phenotypic, chemotaxonomic and phylogenetic characteristics and the results of DNA-DNA hybridization tests, the three novel stains represent two novel species, for which the names Actinopolyspora alba sp. nov. and Actinopolyspora erythraea sp. nov. are proposed. The type strains of the species are YIM 90480(T) ( = DSM 45004(T) = KCTC 19119(T)) and YIM 90600(T) ( = CCTCC M 208247(T) = KCTC 19372(T)). In addition, we propose that Actinopolyspora iraqiensis (Ruan et al., 1994) be transferred to the genus Saccharomonospora as a heterotypic synonym of Saccharomonospora halophila based on present research results.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 61(Pt 7):1693-8. · 2.11 Impact Factor
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ABSTRACT: A gram-positive and aerobic bacterium, designated strain YIM 49065(T), was isolated from rhizospheric soil of Jatropha curcas in Yunnan, China. This isolate formed branched and fragmented mycelia containing ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The major cellular fatty acid profile was characterized by iso-C(14 : 0), iso-C(15 : 0) and iso-C(16 : 0), and the predominant menaquinone was MK-8(H(4)). The DNA G+C content was 69.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequence demonstrated that strain YIM 49065(T) was closely associated with Intrasporangium calvum DSM 43043(T) and Humihabitans oryzae KV-657(T), exhibiting 98.8 % and 98.6 % 16S rRNA gene sequence similarities, respectively. Furthermore, DNA-DNA hybridizations among strains YIM 49065(T), I. calvum DSM 43043(T) and H. oryzae DSM 22331(T) indicated that this isolate represented a novel species in the genus Intrasporangium. On the basis of these data, we proposed one novel species, Intrasporangium mesophilum sp. nov., for strain YIM 49065(T) ( = DSM 23217(T) = CCTCC AA 209077(T)). Additionally, the comparison of biochemical and phylogenetic characters supported the reclassification of Humihabitans oryzae as a separate species within the genus Intrasporangium, Intrasporangium oryzae comb. nov. (type strain KV-657(T) = JCM 15924(T) = NBRC 101802(T) = NRRL B-24470(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 62(Pt 5):1037-41. · 2.11 Impact Factor
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ABSTRACT: A halotolerant actinomycete strain, designated XJEEM 11063(T), was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063(T) grew at pH 6.0-8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28-37 °C) and at salinities of 0-10 % (w/v) NaCl (optimal growth at 0-5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). MK-9(H(4)) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063(T) to the genus Myceligenerans. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense XLG9A10.2(T) (98.3 %) and Myceligenerans crystallogenes DSM 17134(T) (97.0 %). However, it had relatively low values for DNA-DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, Myceligenerans halotolerans sp. nov., is proposed. The type strain is XJEEM 11063(T) ( = DSM 21949(T) = CCTCC AA 208063(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 61(Pt 4):974-8. · 2.11 Impact Factor
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ABSTRACT: A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidase-negative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006(T), was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0-18 % (w/v) NaCl (optimum, 0.5-4.0 %), at pH 6.0-10.5 (optimum, pH 8.0) and at 15-45 °C (optimum, 30 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and C(16 : 0). Strain JSM 082006(T) contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006(T) should be assigned to the genus Bacillus and that it was most closely related to the type strains of Bacillus barbaricus (sequence similarity 99.1 %) and Bacillus arsenicus (97.5 %), followed by those of Bacillus rigui (96.6 %) and Bacillus solisalsi (96.1 %). Phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006(T) represents a novel species of the genus Bacillus, for which the name Bacillus nanhaiensis sp. nov. is proposed; the type strain is JSM 082006(T) ( = DSM 23009(T) = KCTC 13712(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 61(Pt 4):888-93. · 2.11 Impact Factor
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ABSTRACT: A novel xylan-degrading actinomycete, strain YIM 61515(T), was isolated from surface-sterilized leaves of the medicinal plant Maytenus austroyunnanensis. Cells were Gram-positive and non-spore-forming, produced primary branches and formed white to yellowish white colonies on the media tested. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61515(T) was most similar to Promicromonospora aerolata V54A(T) and Promicromonospora vindobonensis V45(T) (99.4 and 99.1% sequence similarity, respectively). The isolate formed a separate lineage in a cluster containing P. aerolata V54A(T). 16S rRNA gene sequence similarity between the isolate and other members of the genus Promicromonospora ranged from 96.3 to 98.4%. Chemotaxonomic data, including major menaquinones, fatty acid compositions and polar lipid profiles, supported the placement of strain YIM 61515(T) in the genus Promicromonospora. DNA-DNA relatedness, physiological and biochemical data showed that strain YIM 61515(T) could be distinguished from members of all known species of the genus Promicromonospora and therefore represented a novel species. The name Promicromonospora xylanilytica sp. nov. is proposed, with YIM 61515(T) (=DSM 21603(T)=CCTCC AA 208046(T)) as type strain.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2011; 62(Pt 1):84-9. · 2.11 Impact Factor
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ABSTRACT: A novel Gram-staining-positive, moderately halophilic, facultatively alkaliphilic, non-motile, catalase-positive, oxidase-negative, endospore-forming, facultatively anaerobic rod, designated JSM 076093(T), was isolated from a sea urchin (Hemicentrotus pulcherrimus) collected from Naozhou Island in the South China Sea. Growth occurred with 0.5-25% (w/v) NaCl (optimum 5-8%) and at pH 6.0-10.5 (optimum pH 8.0) and 5-40 °C (optimum 30-35 °C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The major cellular fatty acids (>10% of the total) were anteiso-C(15:0), anteiso-C(17:0), iso-C(16:0) and iso-C(14:0). The genomic DNA G+C content was 38.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076093(T) belonged to the genus Bacillus and was related most closely to Bacillus hwajinpoensis SW-72(T) (99.1% 16S rRNA gene sequence similarity) and Bacillus algicola KMM 3737(T) (97.3%). The combination of results from the phylogenetic analysis, DNA-DNA hybridization and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 076093(T) represents a novel species of the genus Bacillus, for which the name Bacillus hemicentroti sp. nov. is proposed, with JSM 076093(T) (=DSM 23007(T)=KCTC 13710(T)) as the type strain.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2011; 61(Pt 12):2950-5. · 2.11 Impact Factor
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ABSTRACT: A novel actinobacterium, designated YIM 65623(T), was isolated from a surface-sterilized stem of Artemisia annua L. Strain YIM 65623(T) had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia. Growth occurred with 0-7% (w/v) NaCl (optimum 0-3%), at pH 5.0-9.0 (optimum pH 6.0) and at 10-37 °C (optimum 20-28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623(T) constituted a distinct sublineage within the genus Nocardia and displayed 94.1-98.2% sequence similarity to members of established species in the genus Nocardia. However, DNA-DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623(T) could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623(T) be classified as a representative of a novel species, Nocardia artemisiae sp. nov. The type strain is YIM 65623(T) (=DSM 45379(T) =CCTCC AA 209038(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2011; 61(Pt 12):2933-7. · 2.11 Impact Factor
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ABSTRACT: Two novel thermophilic, spore-forming bacterial strains, T-11(T) and E-112(T), were isolated from hot springs in Tengchong and Eryuan counties of Yunnan province in south-west China. The strains were Gram-stain-positive rods, occurring singly or in chains. Growth of strain T-11(T) was observed between 30 and 75 °C (optimum 50 °C) and at pH 7-11 (optimum pH 8.5), while the temperature range for strain E-112(T) was 35-70 °C (optimum 55 °C) and the pH range was 7.0-11.0 (optimum pH 8.0). The DNA G+C contents of strains T-11(T) and E-112(T) were 41.1 and 42.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarity, the two strains were shown to be related most closely to Anoxybacillus species. The chemotaxonomic characteristics [predominant isoprenoid quinone menaquinone 7 (MK-7); major fatty acids iso-C(15 : 0) and iso-C(17 : 0)] also supported the affiliation of strains T-11(T) and E-112(T) to the genus Anoxybacillus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains T-11(T) and E-112(T) from Anoxybacillus species with validly published names. Strains T-11(T) and E-112(T) therefore represent two novel species, for which the names Anoxybacillus tengchongensis sp. nov. (type strain T-11(T) =CCTCC AB209237(T) =KCTC 13721(T)) and Anoxybacillus eryuanensis sp. nov. (type strain E-112(T) =CCTCC AB209236(T) =KCTC 13720(T)) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2010; 61(Pt 1):118-22. · 2.11 Impact Factor
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ABSTRACT: A novel strain, designated YIM 61452(T), was isolated from the stem of Maytenus austroyunnanensis collected from the tropical rainforest of Xishuangbanna, Yunnan Province, south-west China. The strain was characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61452(T) belonged to the genus Pseudonocardia and was most closely related to Pseudonocardia alni IMSNU 20049(T) (99.5 %), Pseudonocardia antarctica DVS 5a1(T) (99.5 %) and Pseudonocardia carboxydivorans Y8(T) (99.4 %). The chemotaxonomic properties of strain YIM 61452(T), for example MK-8(H(4)) as the predominant quinone system and iso-C(16 : 0) and iso-C(16 : 1) H as the major fatty acids, were also consistent with its classification within the genus Pseudonocardia. Strain YIM 61452(T) could be differentiated from closely related species of the genus Pseudonocardia by phenotypic and genotypic analysis. DNA-DNA hybridization experiments between strain YIM 61452(T) and closely related reference strains further confirmed that strain YIM 61452(T) represented a novel taxon of the genus Pseudonocardia. Therefore, strain YIM 61452(T) represents a novel species, for which the name Pseudonocardia tropica sp. nov. is proposed. The type strain is YIM 61452(T) (=DSM 45199(T)=CCTCC AA 208018(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2009; 60(Pt 11):2524-8. · 2.11 Impact Factor
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ABSTRACT: A thermophilic strain, designated YIM 10002(T), was isolated from a soil sample of Big Empty Volcano in Tengchong county, Yunnan province, south-west China, and a polyphasic approach was used to investigate its taxonomic position. Strain YIM 10002(T) formed endospores on both aerial and substrate mycelia. Whole-cell hydrolysates contained meso-diaminopimelic acid, ribose, xylose and glucose. The major fatty acids were iso-C(15 : 0) and anteiso-C(15 : 0). The predominant menaquinone was MK-9. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides, together with some unknown phospholipids. The G+C content of its genomic DNA was 48.6 mol%. All of these chemotaxonomic data together with morphological characters consistently assigned strain YIM 10002(T) to the genus Laceyella. 16S rRNA gene sequence analysis showed that strain YIM 10002(T) was most closely related to Laceyella sacchari KCTC 9790(T) and Laceyella putida KCTC 3666(T) (99.9 and 98.0 % 16S rRNA gene sequence similarity, respectively). However, strain YIM 10002(T) showed relatively low DNA-DNA relatedness (34.0 and 39.0 %, respectively) with the above strains. Therefore, strain YIM 10002(T) represents a novel species of the genus Laceyella, for which the name Laceyella tengchongensis sp. nov. is proposed. The type strain is YIM 10002(T) (=DSM 45262(T) =CCTCC AA 208050(T)).
International journal of systematic and evolutionary microbiology 11/2009; 60(Pt 9):2226-30. · 2.27 Impact Factor
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ABSTRACT: A Gram-stain-positive, aerobic actinomycete, strain YIM 60513(T), was isolated from the stem of Gloriosa superba L. collected from tropical rainforest at Xishuangbanna, Yunnan Province, south-west China. 16S rRNA gene sequence analysis indicated that strain YIM 60513(T) belonged to the genus Saccharopolyspora and was closely related to Saccharopolyspora gregorii NCIB 12823(T) (99.1 % similarity) and Saccharopolyspora cebuensis SPE 10-1(T) (97.3 % similarity). Data for the predominant quinone [MK-9(H(4))], major fatty acids (iso-C(16 : 0), anteiso-C(17 : 0) and C(17 : 1) cis9) and G+C content of the genomic DNA (71.6 mol%) were similar to those for members of the genus Saccharopolyspora. The level of DNA-DNA relatedness between strain YIM 60513(T) and S. gregorii NCIB 12823(T) was 43 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic characteristics and DNA-DNA hybridization data supported the view that strain YIM 60513(T) should be distinguished from S. gregorii NCIB 12823(T) and S. cebuensis SPE 10-1(T). Strain YIM 60513(T) therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora gloriosae sp. nov. is proposed. The type strain is YIM 60513(T) (=KCTC 19243(T) =CCTCC AA 207006(T)).
International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 5):1147-51. · 2.27 Impact Factor
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ABSTRACT: A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 078133(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Strain JSM 078133(T) was able to grow with 1-23 % (w/v) total salts (optimum, 5-10 %) and at pH 6.0-10.0 (optimum, pH 7.5) and 10-45 degrees C (optimum, 30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(15 : 0). The genomic DNA G+C content of strain JSM 078133(T) was 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078133(T) belongs to the genus Halobacillus, and was related most closely to the type strains of Halobacillus campisalis (97.1 % sequence similarity), Halobacillus alkaliphilus (97.0 %) and Halobacillus yeomjeoni (96.8 %). The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078133(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus salsuginis sp. nov. is proposed. The type strain is JSM 078133(T) (=DSM 21185(T)=KCTC 13236(T)).
International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 10):2505-9. · 2.27 Impact Factor
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ABSTRACT: A novel Gram-negative, slightly halophilic, catalase-positive, oxidase-negative, obligately aerobic, non-sporulating rod-shaped bacterium, designated strain JSM 078169(T), was isolated from a sea urchin (Hemicentrotus pulcherrimus) collected from the South China Sea. Growth occurred with 1-20 % (w/v) total salts (optimum, 3-5 %), at pH 6.0-10.5 (optimum, pH 7.5) and at 4-40 degrees C (optimum, 25-30 degrees C). The major cellular fatty acids were C(18 : 1)omega7c, C(16 : 0) and C(12 : 0) 3-OH. The predominant respiratory quinone was Q-9 and the genomic DNA G+C content was 55.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 078169(T) should be assigned to the genus Halomonas. The sequence similarities between the isolate and the type strains of members of the genus Halomonas were in the range 92.4-97.0 %. The combination of phylogenetic analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078169(T) represents a novel species of the genus Halomonas, for which the name Halomonas zhanjiangensis sp. nov. is proposed, with JSM 078169(T) (=CCTCC AB 208031(T)=DSM 21076(T)=KCTC 22279(T)) as the type strain.
International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 11):2888-93. · 2.27 Impact Factor
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ABSTRACT: A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(6)) (40.8 %), MK-9(H(8)) (38.2 %) and MK-9(H(2)) (8.8 %). The major fatty acids were iso-C(16 : 0) (29.6 %), anteiso-C(15 : 0) (14.0 %) and anteiso-C(17 : 0) (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036(T) (=DSM 41426(T)) and Streptomyces albiaxialis DSM 41799(T), respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187(T) (=NRRL B-24674(T)=CCTCC AA 208049(T)=KCTC 19546(T)).
International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 11):2870-4. · 2.27 Impact Factor
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ABSTRACT: A novel actinomycete strain, designated YIM 61105(T), was isolated from a leaf of Maytenus austroyunnanensis from the tropical rainforest in Xishuangbanna, Yunnan Province, south-west China. A 16S rRNA gene sequence analysis revealed that the organism belonged to the phylogenetic cluster of the genus Nonomuraea and was most closely related to Nonomuraea candida HMC10(T) (98.2 %), 'Nonomuraea aegyptia' S136 (97.9 %), Nonomuraea kuesteri GW 14-1925(T) (97.5 %) and Nonomuraea turkmeniaca DSM 43926(T) (97.4 %). The 16S rRNA gene sequence similarities to other Nonomuraea species were less than 97.4 %. The main chemotaxonomic properties of strain YIM 61105(T), such as the principal amino acid of the peptidoglycan, the predominant menaquinone and the polar lipid profile, supported its classification within the genus Nonomuraea. Strain YIM 61105(T) was also readily differentiated from closely related species on the basis of a broad range of phenotypic properties and DNA-DNA hybridization values. Thus, this isolate is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea antimicrobica sp. nov. is proposed. The type strain is YIM 61105(T) (=DSM 45220(T)=CCTCC AA 208016(T)).
International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 11):2747-51. · 2.27 Impact Factor
