H Takashima

Gifu University, Gihu, Gifu, Japan

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Publications (3)3.13 Total impact

  • H Takashima, H Sakai, T Yanai, T Masegi
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    ABSTRACT: To detect serum antibody against Pasteurella multocida (P. multocida) in infected rabbits. a modified immunoperoxidase assay was applied. An outbreak of P. multocida infection in rabbits started from sudden death. The infected rabbits had severe fibrinous and purulent pneumonia with hemorrhage, and a large number of P. multocida (A:12) was isolated from the trachea and lungs of the animals. Antibodies of IgM and IgG to P. multocida were assessed by immunohistochemical staining using the sera of the animals as primary antibodies and applying them to formalin-fixed, paraffin-embedded sections of P. multocida attached to calf fibrin. IgM antibodies to P. multocida were first detected 7 days after the onset of the disease. IgG antibodies began to rise on the 7th or 14th day. These results suggested that the modified immunoperoxidase assay could detect antibodies against P. multocida.
    Journal of Veterinary Medical Science 03/2001; 63(2):171-4. · 0.88 Impact Factor
  • H Takashima, H Sakai, T Yanai, T Masegi
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    ABSTRACT: Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.
    Journal of Veterinary Medical Science 07/1999; 61(6):713-6. · 0.88 Impact Factor
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    ABSTRACT: Bromodeoxyuridine (BrdU), a non-radioactive thymidine analogue, was administered to 15 cattle at a dosage of 1-10 mg/kg intravenously or intraperitoneally to demonstrate S-phase cells in the tissues. The organs and tissues were fixed in 10% neutral buffered formalin or in 70% ethanol, sectioned, denatured with hydrochloric acid, and treated with monoclonal antibody against BrdU. Immunohistochemical methods were used to "visualize" BrdU-labelled nuclei. BrdU-positive cells were satisfactorily demonstrated in both formalin- and ethanol-fixed tissues of animals given doses of 2 mg/kg or over, by either route of administration. Large numbers of BrdU-positive cells indicative of active cell production were found in the basal region of the stratified squamous epithelium, the neck between gastric pits and gastric glands in the abomasum, and the crypts of Lieberk├╝hn of the small and large intestines. Moderate numbers of positive cells were observed amongst inflammatory cells in cases of nephritis and in granulation tissue. Numerous positive cells were detected in leukaemia cells. The study showed that BrdU can be used to measure proliferative S-phase cells in cattle, as in human beings, mice and rats.
    Journal of Comparative Pathology 05/1996; 114(3):265-72. · 1.38 Impact Factor

Publication Stats

15 Citations
3.13 Total Impact Points

Institutions

  • 1996–2001
    • Gifu University
      • Laboratory of Veterinary Pathology
      Gihu, Gifu, Japan