H Abe

Niigata University, Niahi-niigata, Niigata, Japan

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Publications (107)149.57 Total impact

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    ABSTRACT: The caveolin 1 to caveolin 2 (CAV1-CAV2) gene region on chromosome 7q31 has been reported to be associated with susceptibility to primary open angle glaucoma (POAG) and normal tension glaucoma (NTG) in previous studies. We investigated whether genetic variants in the CAV1-CAV2 region are associated with NTG in Japanese patients. Two hundred and ninety-two Japanese patients with NTG and 352 Japanese healthy controls were recruited. We genotyped three single-nucleotide polymorphisms; that is, rs1052990, rs4236601, and rs7795356, in the CAV1-CAV2 gene region and assessed the allelic diversity among cases and controls. The frequency of the minor allele (G) of rs1052990 was significantly decreased in NTG cases compared with controls (P=0.014, OR=0.71), whereas NTG or POAG cases had a significantly higher frequency of the allele than controls in previous studies. Conversely, rs7795356 did not show any significant association with NTG cases, and rs4236601 was monomorphic in the Japanese study population. Our findings did not correspond with previous positive results, suggesting that CAV1-CAV2 variants studied in the present study are not important risk factors for NTG susceptibility in all populations. Further studies are needed to elucidate the possible contribution of the CAV1-CAV2 region to the development of glaucoma.Eye advance online publication, 7 June 2013; doi:10.1038/eye.2013.123.
    Eye (London, England) 06/2013; · 1.97 Impact Factor
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    ABSTRACT: To investigate whether the GLC3A locus harboring the CYP1B1 gene is associated with normal tension glaucoma (NTG) in Japanese patients. One hundred forty-two Japanese patients with NTG and 101 Japanese healthy controls were recruited. Patients exhibiting a comparatively early onset were selected as this suggests that genetic factors may show stronger involvement. Genotyping and assessment of allelic diversity was performed on 13 highly polymorphic microsatellite markers in and around the GLC3A locus. There were decreased frequencies of the 444 allele of D2S0416i and the 258 allele of D2S0425i in cases compared to controls (P = 0.022 and P = 0.034, respectively). However, this statistical significance disappeared when corrected (Pc > 0.05). We did not find any significant association between the remaining 11 microsatellite markers, including D2S177, which may be associated with CYP1B1, and NTG (P > 0.05). Our study showed no association between the GLCA3 locus and NTG, suggesting that the CYP1B1 gene, which is reportedly involved in a range of glaucoma phenotypes, may not be an associated factor in the pathogenesis of NTG.
    Clinical ophthalmology (Auckland, N.Z.) 02/2009; 3:183-8.
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    ABSTRACT: This study sought to elucidate the effects of timolol and dorzolamide on intraocular pressure (IOP) and retinal ganglion cell (RGC) death in an experimental model of glaucoma in rat. Mild elevation of IOP was induced in rats by intracameral injection of India ink and subsequent laser trabecular photocoagulation. IOP was measured before the surgical procedures and weekly thereafter. Timolol (0.5%), timolol XE (0.5%), dorzolamide (1%), and artificial tears (vehicle) were topically applied daily. Retinal sections were prepared for histology to determine RGC number. Timolol, timolol XE, and dorzolamide induced a significant reduction in IOP (p<0.05) and counteracted the reduction in RGC number that occurred in vehicle treated glaucomatous eyes (p<0.05). The coefficient of correlation between RGC number and IOP was significant in the dorzolamide treated group (r = -0.908, p<0.005), but not in other groups (p>0.05). Both timolol formulation and dorzolamide reduced IOP and protected RGCs in a rat model of experimental glaucoma. It cannot be ruled out that timolol might protect RGCs by additional mechanisms other than simply lowering of IOP.
    British Journal of Ophthalmology 04/2005; 89(4):504-7. · 2.73 Impact Factor
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    British Journal of Ophthalmology 04/2003; 87(3):371-2. · 2.73 Impact Factor
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    ABSTRACT: We report a patient with persistent hyperplastic primary vitreous(PHPV) who presented with acute angle-closure glaucoma in his adult life. A 30-year-old man had an attack of acute angle-closure glaucoma associated with retrolenticular fibrous tissue, atrophic retina, and elongated cilliary process in his right eye. Ultrasound biomicroscopy(UBM) study showed iris bowing, shallow anterior chamber, and elongated cilliary body which were being pulled by the retrolenticular mass. The posterior chamber was normal. Although the mechanisms of secondary angle-closure glaucoma in PHPV are complicated, we suspected pupillary block resulting from constriction by the retrolenticular mass in this case.
    Nippon Ganka Gakkai zasshi 11/2001; 105(10):711-5.
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    ABSTRACT: To estimate retinal function in Bietti crystalline chorioretinal dystrophy using the electroretinogram. In this observational case series, the scotopic and photopic electroretinograms in three Japanese female patients (case 1, 55 years old; case 2, 56 years old; case 3, 47 years old) who showed bilateral crystalline retinal deposits but no corneal deposits were recorded. The rod and cone a-waves were analyzed by using the method described by Hood and Birch (1995, 1997). The parameters Rm(p3) (maximum a-wave amplitude) and S (sensitivity) were calculated. In case 1, the rod Rm(p3) was decreased in both eyes. The rod S in the right eye was within the normal range, but that in the left eye was significantly reduced. Although the cone Rm(p3) was decreased, the cone S was within the normal range. In case 2, the rod and cone Rm(p3) was reduced, but the rod and cone S was within the normal range in both eyes. In case 3, the rod and cone Rm(p3) and S were within the normal range. Electroretinograms illustrated different disease stages, however, no eye with normal Rm(p3) and decreased S was found in rods and cones. In the early stages of this disease, decreased numbers of photoreceptors and/or outer segment shortening may be present while phototransduction remains normal. As the damage to the retina progresses, phototransduction becomes severely affected. Because reduced cone S was not observed in our cases, cones may be less involved than rods in this disease.
    American Journal of Ophthalmology 10/2001; 132(3):395-402. · 4.02 Impact Factor
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    ABSTRACT: We studied the control of intraocular pressure(IOP) by various types of blebs after non-penetrating trabeculectomy(NPT) and the difference between bleb formation after penetrating trabeculectomy(PT) and that after NPT. The filtering blebs of 45 yeys from 40 patients after NPT were studied using ultrasound biomicroscopy. They were grouped into four types, and the space under the scleral flap was classified into three types. The filtering blebs and the space under the scleral flap were correlated with IOP level. Overall, 40% of the blebs were L(low-reflective) type, 16% H(high-reflective) type, 16% E (encapsulated) type, and 29% F(flattened) type, but in good IOP control cases 59% of the blebs were L type, 14% H type, 14% E type, and 14% F type. L type blebs were found in 94% of eyes with good IOP control. Though filtering blebs of the L type could produce sufficient IOP reduction, blebs after NPT showed a greater tendency to become flattened than after PT. Additional systematic therapy must be designed to maintain the L type of filtering blebs after NPT.
    Nippon Ganka Gakkai zasshi 08/2001; 105(7):447-51.
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    ABSTRACT: Vergence eye movements undergo adaptive recalibration in response to a training stimulus in which the initial disparity is changed just after vergence begins (the double-step paradigm). In the present study the changes in the dynamic properties of convergence, speed and acceleration, were examined by using this double-step paradigm, before and after adaptation. Four normal subjects participated. Three-dimensional visual stimuli were provided by a head-mounted display with two liquid crystal diode (LCD) panels. To induce adaptation, a double step of disparity was used: an initial step from distances of 2 to 1 m was followed by a second step to distances of 0.7 m ("increasing paradigm") or 1.4 m ("decreasing paradigm") after a constant period of 0.2 seconds. The dynamic properties of vergence were compared before and after 30 minutes of training with these paradigms. Peak velocity of convergence became significantly greater (increasing paradigm) or smaller (decreasing paradigm) after 30 minutes' training. Changes in the dynamic properties of convergence were also obvious in phase-plane (velocity versus position) and main sequence (peak velocity versus amplitude) plots. Further analysis revealed that adaptive increases in vergence velocity were accomplished by an increase in the duration of the acceleration period, whereas adaptive decreases were induced by a decrease in the maximum value of acceleration. The pattern of change in the dynamic characteristics of vergence after adaptation was similar to that of saccades and the initiation of pursuit eye movements, suggesting common neural mechanisms for adaptive changes in the open-loop control of eye movements.
    Investigative Ophthalmology &amp Visual Science 07/2001; 42(7):1479-86. · 3.44 Impact Factor
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    ABSTRACT: To determine the role in the eye of chondromodulin (ChM)-I, which has been identified in cartilage as an angiogenic inhibitor, the expression and localization and a possible function of ChM-I were investigated. Expression and localization of ChM-I in rat eyes were examined by RNase protection assay and in situ hybridization and by immunostaining, using an antibody against a synthetic peptide. The effect of recombinant ChM-I on tube morphogenesis of retinal endothelial cells was examined in culture. The rat ChM-I gene was determined to encode the open reading frame of 334 amino acid residues, and ChM-I mRNA was exclusively expressed in cartilage, eye, and cerebellum in rats. ChM-I mRNA expression was evident in the iris-ciliary body, retina, and scleral compartments, but not in other compartments of the eye. In situ hybridization revealed mRNA expression in the ganglion cells, inner nuclear layer cells, and pigment epithelium in the retina and in the nonpigment epithelium of the ciliary body. Immunoreactive ChM-I was present in these cells and also in the vitreous body. Western blot analysis detected an approximately 25-kDa band of ChM-I presumed as a secretory form in the aqueous humor and vitreous body and an approximately 37-kDa band as a precursor form in the retina. Recombinant human ChM-I inhibited tube morphogenesis of human retinal endothelial cells in vitro. These observations indicate a potential role for ChM-I in inhibition of angiogenesis in the rat eye.
    Investigative Ophthalmology &amp Visual Science 06/2001; 42(6):1193-200. · 3.44 Impact Factor
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    ABSTRACT: The initial acceleration of pursuit in the open-loop period is under adaptive control and undergoes motor learning. The current study was undertaken to examine the hypothesis that the direction of pursuit initiation can also be adaptively modified. Four neurologically and ophthalmologically normal subjects participated in the experiment. A modified step-ramp paradigm was used to induce cross-axis adaptation, in which a ramp target changed its direction orthogonally just after the target crossed the center. Four direction changes were tested in separate experiments: left to up, left to down, down to left, and up to left. During a 30-minute adaptation session, the target moved in one of two randomly chosen directions (right to left or up to down) at one of two randomly chosen speeds (15.6 or 22.3 deg/sec), but the target changed orthogonally in only one direction. A linear regression fit to the initial 100-msec segment of the pursuit trace was used to determine the direction of pursuit initiation. In all cases, an adaptive change in pursuit initiation was gradually induced in the direction called for by the training paradigm. Adaptation was usually completed (90 degrees shift) within the 30-minute training session but declined quickly to an approximate 30 degrees -shift after training. The latency and vectorial amplitude of the initial acceleration remained unchanged. The adaptation was specific for the direction but not the velocity of the target. This study showed that the direction of pursuit initiation is under adaptive control, as has been shown for saccadic eye movements and the vestibulo-ocular reflex.
    Investigative Ophthalmology &amp Visual Science 04/2001; 42(3):668-74. · 3.44 Impact Factor
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    ABSTRACT: To investigate the usefulness of second-order multifocal electroretinograms (MERGs) for detecting inner retinal disorders. The MERG from 5 patients with branch retinal artery occlusion (BRAO) was recorded. Twelve eyes of 12 normal subjects were also tested. MERGs were recorded using 61 hexagons. Bright flash ERGs were also recorded to measure the oscillatory potentials (OP). Root mean square (RMS) measures of the local first- and second-order MERGs (fMERG and sMERG) were compared in the affected and unaffected areas. The first negative trough (N1) and first positive peak (P1) were also used for measuring the amplitudes and latencies of the fMERG. The fMERG RMS-amplitudes decreased significantly (r = 0.56, P: < 0.05) in the affected area compared with normal values. The fMERG latencies of N1 and P1 increased significantly (P: < 0.05) in the affected area. Furthermore, the sMERG RMS-amplitudes decreased almost to the noise level (r = 0.28, P: < 0.001) in the affected areas. The interocular ratio of the sMERG RMS-amplitudes (affected/normal) significantly correlated with that of the fMERG (r = 0.69, P: < 0.001). The fMERG latencies significantly correlated with the sMERG RMS-amplitude (r = 0.37 approximately 0.69, P: < 0.05 approximately 0.001), but only began to increase after a 30% to 50% loss of the sMERG amplitude. The summed OP amplitude decreased to the same extent as the sMERG in the affected eye (0.5 of the normal eye). Although the fMERG amplitude and latency were significantly changed, the sMERG was much more affected by BRAO. The marked reduction of the sMERG in the affected area strongly suggested its main source was from the more inner layers of the retina compared to the fMERG. The sMERG appeared to be a sensitive indicator of inner retinal dysfunction.
    Investigative Ophthalmology &amp Visual Science 02/2001; 42(1):298-304. · 3.44 Impact Factor
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    ABSTRACT: We recorded multifocal electroretinograms (M-ERG) in patients with branch retinal artery occlusion (BRAO) and compared the waveforms in the area of retinal artery occlusion with those in the normal area, to evaluate the influence of the damage to the inner retina shown by M-ERG responses. Three patients who had normal visual acuity and visual field loss of more than one quadrant due to BRAO were examined. The central 50 degrees of ocular fundus was stimulated through dilated pupils by an array of 103 hexagonal elements for 4 minutes. The waveforms of the first order and second order kernel responses of M-ERG in the area of the retinal artery occlusion were compared with those of the vertically symmetrical, normally perfused area of the same eye. The amplitude of the averaged tracing decreased in the first negative wave (N1), first positive wave (P1), and second negative wave (N2) in the first order kernel responses in the area of retinal artery occlusion in comparison with the normally perfused area. Furthermore, prolongation of latency was noted for N1, P1, and N2 in the same area. Second order kernel responses were not detected in the area of the retinal artery occlusion. The damage to the inner retina affected parts of N1, P1, and N2 of the first order kernel responses, with N2 being the most seriously affected. Furthermore, second order kernel responses clearly reflected the condition of the inner retina.
    Japanese Journal of Ophthalmology 01/2001; 45(5):516-22. · 1.27 Impact Factor
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    ABSTRACT: The distribution of the cell adhesion glycoproteins, laminin, fibronectin, tenascin, vitronectin, thrombospondin, and entactin/nidogen, was examined in the human lamina cribrosa. Frozen sections of the optic nerve head from 7 normal human elderly donors were stained by immunohistochemistry. All six glycoproteins were detected in this tissue. While laminin and entactin/nidogen were observed linearly, reflecting the localization of basement membranes, fibronectin was identified diffusely. Marked tenascin immunoreactivity was apparent in the lamina cribrosa, but little or no tenascin staining was detected in the sclera. Vitronectin showed a fine fibrillar staining pattern in the lamina cribrosa, and, to a lesser extent, in the sclera and pial septa. Thrombospondin staining was apparent only in the sclera and the lamina cribrosa, which traversed the optic nerve. These results indicate that extracellular matrix components in the lamina cribrosa differ from those in the sclera or pial septa. This study is the first report that the human lamina cribrosa includes vitronectin and thrombospondin.
    Japanese Journal of Ophthalmology 01/2001; 45(4):363-7. · 1.27 Impact Factor
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    ABSTRACT: To determine if multiple states for the initiation of pursuit, as assessed by acceleration in the "open-loop" period, can be learned and gated by context. Four normal subjects were studied. A modified step-ramp paradigm for horizontal pursuit was used to induce adaptation. In an increasing paradigm, target velocity doubled 230 msec after onset; in a decreasing paradigm, it was halved. In the first experiment, vertical eye position (+/-5 degrees ) was used as the context cue, and the training paradigm (increasing or decreasing) changed with vertical eye position. In the second experiment, with vertical position constant, when the target was red, training was decreasing, and when green, increasing. The average eye acceleration in the first 100 msec of tracking was the index of open-loop pursuit performance. With vertical position as the cue, pursuit adaptation differed between up and down gaze. In some cases, the direction of adaptation was in exact accord with the training stimuli. In others, acceleration increased or decreased for both up and down gaze but always in correct relative proportion to the training stimuli. In contrast, multiple adaptive states were not induced with color as the cue. Multiple values for the relationship between the average eye acceleration during the initiation of pursuit and target velocity could be learned and gated by context. Vertical position was an effective contextual cue but not target color, implying that useful contextual cues must be similar to those occurring naturally, for example, orbital position with eye muscle weakness.
    Investigative Ophthalmology &amp Visual Science 12/2000; 41(12):3763-9. · 3.44 Impact Factor
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    ABSTRACT: Purpose: We attempted to apply a newly developed image-analysis system for measurement and analysis of nystagmus.Method: Eye movements were recorded by digital video through a head-mounted charge coupled device (CCD) camera. The recorded movie was converted into black and white in order to detect the area of the pupil. Horizontal and vertical eye positions were determined by calculating the centroid of the pupil. Torsional angle was calculated using the iris striate pattern around the pupillary margin.Results: The parameters (amplitude, cycle, etc.) of nystagmus were calculated easily by the new image-analysis system from the recorded images. As examples, the foveation period was measured accurately in a case of jerky-type congenital nystagmus. Very regular cycles of intorsional attack period were revealed in a case of superior oblique myokymia. A case of cork-screw-like nystagmus showed a characteristic combination of large and small cycles unassociated with torsion.Conclusion: This image-analysis system was useful for quantitative analysis of nystagmus, and especially for measurement of torsion. Detailed waveforms and specific rhythms of nystagmus, which could not be recognized by observation, were demonstrated by this system.
    Japanese Journal of Ophthalmology 12/2000; 44(6):696-697. · 1.27 Impact Factor
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    ABSTRACT: To report blood flow in the optic nerve head between the right and left eyes or the superior and inferior neuroretinal rims in normal volunteers using laser speckle flowgraphy. This prospective study included 120 eyes of 60 normal volunteers (mean age, 50.0 +/- 16.9 years; range, 21 to 77 years). The square blur rate was measured by laser speckle flowgraphy (Kyushu Institute of Technology, Iizuka, Japan). The sequence of eye measurements was randomized. In each eye, measurements were taken at the neuroretinal rim away from visible vessels. Linear regression analysis, paired two-tailed t test, and two-way analysis of variance were used for statistical analysis. P values less than.05 were accepted as statistically significant. There was a significant correlation in square blur rate between the right and left eyes (r = 0.587, P <.001). Square blur rate in the superior temporal neuroretinal rim significantly correlated with that in the inferior temporal neuroretinal rim in each of the right (r = 0.546, P <.001) and left (r = 0.465, P <.001) eyes. Square blur rate in the right eye was higher than that in the left eye (P =.049). Square blur rate in the superior neuroretinal rim was higher than that in the inferior neuroretinal rim in both the right (P =.035) and left (P =. 005) eyes. There were statistically significant differences of optic nerve head blood flow in normal volunteers using laser speckle flowgraphy between the right and left eyes and between the superior and inferior temporal neuroretinal rims. These normal data can be used for understanding physiological ocular hemodynamics.
    American Journal of Ophthalmology 11/2000; 130(5):606-10. · 4.02 Impact Factor
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    ABSTRACT: We examined ultrastructurally the localization of myocilin (formerly called trabecular meshwork inducible glucocorticoid response, or TIGR) protein in cultured human trabecular meshwork (TM) cells and in normal human TM tissues. The TM, a specialized tissue located at the chamber angle of the eye, is believed to be responsible for the development of glaucoma. The myocilin gene has been directly linked to both juvenile and primary open-angle glaucomas, and multiple mutations have been identified. Human TM cells were treated with 0.1 mM of dexamethasone (DEX) to induce myocilin expression. This protein was immunolocalized by colloidal gold electron microscopy using an anti-human myocilin polyclonal antibody. Double labeling with different sizes of gold particles was also performed with additional monoclonal antibodies specific for cell organelles and structures. In both DEX-treated and untreated cultured cells, myocilin was associated with mitochondria, cytoplasmic filaments, and vesicles. In TM tissues, myocilin was localized to mitochondria and cytoplasmic filaments of TM cells, elastic-like fibers in trabecular beams, and extracellular matrices in the juxtacanalicular region. These results indicate that myocilin is localized both intracellularly and extracellularly at multiple sites. This protein may exert diverse biological functions at different sites.
    Journal of Histochemistry and Cytochemistry 11/2000; 48(10):1321-30. · 2.26 Impact Factor
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    ABSTRACT: To examine collagen fibrils in 3 nanophthalmos sclerae and to compare them with normal control sclerae morphometrically. Three cases of nanophthalmos associated with uveal effusion were studied. When sclerectomy was performed, scleral specimens were collected and fixed with 3% glutaraldehyde/2.5% paraformaldehyde. After epon-embedding and ultrathin sectioning, they were examined by transmission electron microscopy. Collagen fibrils from both nanophthalmos and normal control sclerae were compared in diameters and numbers per micron 2 areas. All scleral tissues from the three cases were associated with irregularly woven and unclear collagen bundles. Several abnormal findings, such as twisting or fraying, were also detected in a few collagen fibrils. The diameter and density of normal-appearing collagen fibrils that occupied most areas of nanophthalmos sclerae were the same as those from normal control sclerae morphometrically. Although nanophthalmos sclerae even with uveal effusion showed thick irregular collagen bundles and a few abnormal collagen fibrils, most collagen fibrils appeared the same as normal controls.
    Nippon Ganka Gakkai zasshi 11/2000; 104(10):706-10.
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    ABSTRACT: Superior oblique myokymia is a microtremor of the eye that causes monocular torsional oscillopsia. A modified Harada-Ito procedure was used to treat a case of the disease in a 20-year-old woman. The authors used video-image analysis pre- and postoperatively to evaluate the effect of the surgery on abnormal torsional eye movements. This analysis revealed that before surgery, the abnormal torsional movement had a very regular cycle (duration of attack, 8.0 +/- 0.5 s; time interval between attacks, 18.7 +/- 3.2 s; n = 9). After the surgery, amplitude of the abnormal torsional eye movement was reduced, and the oscillopsia had subjectively improved, although the movement cycle remained unchanged. The authors' video-image analysis, which used iris striation, proved to be a useful method for clinical measurement of torsional eye movements.
    Journal of Neuro-Ophthalmology 10/2000; 20(3):163-5. · 1.63 Impact Factor
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    ABSTRACT: To elucidate the origin of the abnormal fundus reflex in Oguchi disease. The ocular fundus of a 63-year-old woman who showed a homozygous arrestin 1147delA mutation was observed by scanning laser ophthalmoscopy with the use of an argon blue laser (wavelength, 488 nm), a helium-neon laser (633 nm), and an infrared laser (780 nm). Diffuse, fine, white particles, which do not exist in normal subjects, were clearly demonstrated only with the helium-neon laser. After 4-hour dark adaptation, the abnormal particles disappeared, but then they reappeared gradually during 30 minutes of light adaptation, in accordance with the golden metallic reflex. The white particles found by helium-neon laser could be the origin of the abnormal fundus reflex in Oguchi disease.
    American Journal of Ophthalmology 10/2000; 130(3):359-61. · 4.02 Impact Factor

Publication Stats

1k Citations
149.57 Total Impact Points


  • 1993–2013
    • Niigata University
      • • Division of Ophthamology and Visual Sciences
      • • Department of Ophthalmology and Otolaryngology
      • • Faculty of Medicine
      • • School of Medicine
      Niahi-niigata, Niigata, Japan
  • 1999–2000
    • Nihon Fukushi University
    • Showa University
      • First Department of Surgery
      Shinagawa, Tōkyō, Japan
    • Hokkaido University
      • Graduate School of Pharmaceutical Sciences
      Sapporo-shi, Hokkaido, Japan
  • 1990–1999
    • St. Marianna University School of Medicine
      • Department of Cardiovascular Surgery
      Kawasaki Si, Kanagawa, Japan