Haluk Guriz

Ankara University, Engüri, Ankara, Turkey

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Publications (7)8.15 Total impact

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    ABSTRACT: Objective: Acute gastroenteritis is an important cause of morbidity and mortality in developing countries. Children under 5 years of age are more commonly affected, while 80% of deaths occur in children under 2 years of age. Escherichia coli, Salmonella spp., and Shigella spp. are the most common causative bacterial pathogens. Since the summer of 2014, a large number of Salmonella and Shigella gastroenteritis cases have occurred in our region, which is considered as a small local outbreak. We aim to report the features and antibiotic susceptibility of microorganism isolates obtained during this epidemic. Material and Methods: Between July and September 2014, patients who were admitted to pediatric clinics and emergency services on account of acute gastroenteritis lasted less than 15 days were included in this retrospective study. Stool microscopy, stool cultures, and blood cultures were performed. Salmonella and Shigella strains were subjected to antimicrobial susceptibility testing. Results: A total of 2425 patients were admitted because of acute gastroenteritis during the study period. Salmonella spp. and Shigella spp. were isolated in 113 children. The mean age of the children was 91.5±60.3 months (4-213 months). Of the 113 patients, 56% were male and 44% were female. Shigella spp. were found in 77 patients (3.2%), and Salmonella spp. were found in 36 patients (1.5%). Salmonella enteritidis was detected in 29 patients (80.5%), whereas Salmonella typhimurium was detected in 3 patients (8.3%). In addition, Shigella sonnei was identified in 63 patients (81.8%) and Shigella flexneri in 8 patients (10.3%). In contrast to ceftriaxone susceptibility in Salmonella strains, 9.1% of Shigella strains were found to be resistant to ceftriaxone. Ciprofloxacin resistancewas found in 5.6% and 1.3% strains of Salmonella and Shigella spp. respectively. Although the ampicillin resistance values of 2 strains were close, trimethoprim-sulfamethoxazole (TMP) resistance was found in 89.6% Shigella strains. Conclusion: Salmonella and Shigella are common bacterial pathogens. Resistance patterns must be known to select the most effective treatment. According to our study, ceftriaxone and ciprofloxacin appear to be the first choice of empirical treatment for acute bacterial gastroenteritis. Additionally, if the resistance states of ampicillin and TMP are known, these drugs can also be used as empirical treatment.
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    ABSTRACT: Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood-culture systems reduce the time needed to detect positive cultures, and reduce specimen handling. The false-positive rate of such systems is 1-10%. In this study, the presence of pathogens in 'false-positive' bottles obtained from BACTEC 9050 (Becton Dickinson) and BacT/Alert (Biomérieux) systems was investigated by eubacterial and fungal PCR. A total of 169 subculture-negative aerobic blood-culture bottles (104 BacT/Alert and 65 BACTEC) were evaluated. Both fungal and eubacterial PCRs were negative for all BACTEC bottles. Fungal PCR was also negative for the BacT/Alert system, but 10 bottles (9.6%) gave positive results by eubacterial PCR. Sequence analysis of the positive PCR amplicons indicated the presence of the following bacteria (number of isolates in parentheses): Pasteurella multocida (1), Staphylococcus epidermidis (2), Staphylococcus hominis (1), Micrococcus sp. (1), Streptococcus pneumoniae (1), Corynebacterium spp. (2), Brachibacterium sp. (1) and Arthrobacter/Rothia sp. (1). Antibiotic usage by the patients may be responsible for the inability of the laboratory to grow these bacteria on subcultures. For patients with more than one false-positive bottle, molecular methods can be used to evaluate the microbial DNA in these bottles. False positives from the BACTEC system may be due to elevated patient leukocyte counts or the high sensitivity of the system to background increases in CO(2) concentration.
    Journal of Medical Microbiology 02/2006; 55(Pt 1):53-7. DOI:10.1099/jmm.0.46196-0 · 2.25 Impact Factor
  • Ergin Ciftci · Haluk Guriz · Ahmet Derya Aysev ·
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    ABSTRACT: Terbinafine is an antifungal drug known to have also antibacterial activity against certain Gram-positive and Gram-negative bacteria. It seems that antibacterial and antifungal activity of terbinafine may have an advantage in the treatment of mixed fungal and bacterial superficial skin infections. Nevertheless, clinical relevance of the antibacterial part of its action has not been investigated efficiently. To compare the efficacy and safety of terbinafine with those of mupirocin, which has already proven antibacterial action, in the treatment of impetigo. Children clinically diagnosed as having impetigo were treated with topical mupirocin or topical terbinafine in a randomized fashion. Patients' lesions were examined clinically on days 0, 4, 7, 10 and bacteriologic cultures were obtained on days 0 and 10. A total of 62 patients were included in the study. Forty-eight of these patients were eligible for the efficacy and safety analysis. Twenty-five and 23 patients were treated with mupirocin and terbinafine, respectively. The clinical cure rates were 100% for the mupirocin group and 70% for the terbinafine group (p < 0.05). The bacteriological eradication rate for mupirocin-treated children was 100% and that for terbinafine-treated children was 78% (p < 0.05). Presence of bullous lesions appeared to be a factor for poor clinical outcome in the terbinafine group. Mild local adverse effects were noted in a small percentage of patients in each group. Antibacterial activity of terbinafine is not strong enough to be an alternative in the treatment of impertigo. It is advisable that terbinafine could be used in combination with an antibacterial drug for superficial skin infections caused by both fungi and bacteria.
    The Indian Journal of Pediatrics 09/2002; 69(8):679-82. DOI:10.1007/BF02722704 · 0.87 Impact Factor
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    ABSTRACT: Candida dubliniensis is one of the Candida species which was first recognized in 1995. The yeast was misidentified because of its phenotypic similarities with Candida albicans. In this study, blood samples of patients from various departments at Ankara University Medical Faculty between January 1996 and September 2000 were investigated for distribution of Candida spp. and presence of C. dubliniensis. Ninety-eight culture positive fungi were included in the study. Phenotypic tests for identification of C. dubliniensis and tests for differentiation of the yeast from C. albicans, such as colony morphology on Staib agar, growth at 42 degrees C and 45 degrees C, beta-glucosidase activity and carbohydrate assimilation, were carried out. Sixty-four of the isolates produced germ tubes and chlamydospores, and none of them had the phenotypic characteristics of C. dubliniensis. Further large-scale studies of specific patient groups are necessary to reveal the etiologic importance of this yeast.
    Apmis 06/2002; 110(5):391-5. DOI:10.1034/j.1600-0463.2002.100504.x · 2.04 Impact Factor
  • A D Aysev · H Guriz · B Erdem ·
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    ABSTRACT: 160 Salmonella strains were isolated from children at the paediatrics department of Ankara University. 48.1% of the isolates were Salmonella enteritidis, 41.9% Salmonella typhimurium and 10% other serotypes. For the analysis of data, the study period was divided into 2 periods: 1993-95 and 1996-99. A decline in the isolation rate of S. typhimurium (from 63.1% to 30.1%) and rapid rise in S. enteritidis (from 31.6% to 57.3) was observed during the review period. However, for S. typhimurium isolates, the 5-drug (ampicillin, chloramphenicol, streptomycin, tetracycline and sulfonamides) pattern of resistance was increased from 13.5% to 38.7% in the second period. Since S. enteritidis and 5-drug-resistant S. typhimurium have also increased in other countries, their pandemic spread in humans indicates the continuing importation and exportation of these pathogens.
    Infectious Diseases 02/2001; 33(6):420-2. · 1.50 Impact Factor
  • A D Aysev · H Guriz ·
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    ABSTRACT: 289 Shigella strains were isolated from children at the paediatrics department of Ankara University. 75% of the isolates were S. sonnei and 24.8% were S. flexneri. Each strain was tested for resistance to 9 antimicrobial agents. 79% of the isolates were resistant to streptomycin (S), 56% to tetracycline (T), 55.7% to trimethoprim-sulfamethoxazole (SXT), 27.7% to ampicillin (Am) and 19.7% to chloramphenicol (C). None of the isolates was resistant to ciprofloxacin, nalidixic acid, cephalothin, ampicillin-sulbactam and ceftriaxone. 56% of the isolates were resistant to 3 or more antimicrobial agents. The most frequent pattern of resistance of S. sonnei and S. flexneri strains was SXT, T, S (39.6%) and Am, SXT, T, S, C (48.6%), respectively (p < 0.0001). These results demonstrate that trimethoprim-sulfamethoxazole should not be used in the treatment of shigellosis.
    Infectious Diseases 02/1998; 30(4):351-3. · 1.50 Impact Factor
  • E. Ciftci · U. Doru · H. Guriz · A. Derya Aysev · I. Erdal ·