H Guo

Publications (2)3.27 Total impact

• Article: Mapping, expression, and association study of the bovine PSMC1 gene.

  H Guo, W-S Liu, A Takasuga, K Eyer, E Landrito, S-Z Xu, X Gao, H-Y Ren
  [show abstract] [hide abstract]
  ABSTRACT: The 26S ATP-dependent protease is composed of a 20S catalytic proteasome and two PA700 regulatory modules; it plays a central role in many regulatory pathways, such as cell cycle regulation, differentiation, and apoptosis. The PA700 complex is composed of multiple subunits, including at least six related ATPases and approximately 15 non-ATPase polypeptides. PSMC1 (proteasome 26S subunit, ATPase, 1) is one of these ATPases. In this study, we amplified a fragment of 507 bp from intron 9 of the bovine PSMC1 gene and found a SNP (G/A) at position 216 in the PCR fragment. Genotyping of 138 animals from four beef breeds revealed that the average frequency for allele A (G-base) was 0.4271 (0.3269-0.5517); for allele B (A-base) it was 0.5729 (0.4483-0.6731). This SNP is significantly associated with average daily feed intake (P < 0.01), average daily gain, finishing average daily gain, body length, ratio of feed to meat, backfat thickness, and loin-muscle area (P < 0.05). Our experimental data showed that animals with an AA genotype have a significantly lower food intake, grow faster, are longer in the body, and have less backfat and bigger loin muscle; hence, their ratio of feed to meat is significantly lower. We believe that the PSMC1 SNP is a potential candidate marker for marker-assisted selection in these traits. We also found that the bovine PSMC1 gene was expressed mainly in lung, testis, and spleen. In addition, we mapped the bovine PSMC1 gene on BTA10 by an RH mapping method.
  Biochemical Genetics 02/2008; 46(5-6):347-55. · 0.86 Impact Factor
• Article: Mapping of the CCK, PSMC2, PSMC4, PSMD1, CPB1 and PSPH genes in cattle.

  H Guo, W-S Liu, A Takasuga, K Eyer, E Landrito, S-Z Xu, X Gao, H-Y Ren
  Animal Genetics 03/2006; 37(1):73-5. · 2.40 Impact Factor