Gang Xu

Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Hua-yang, Sichuan, China

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Publications (14)5.77 Total impact

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    ABSTRACT: To better characterize spindle cell metaplastic carcinoma (SpCMC) of breast, a rare variant of breast cancer that has been classified under the broad rubric of metaplastic carcinoma. We presented herein 19 cases of metaplastic breast carcinoma with dominant spindle cell component. All cases were clinically of breast origin, showed more than 80% spindle morphology, 10 cases exhibited pure spindled morphology, 8 contained invasive ductal carcinoma (IDC) and 1 presented with ductal carcinoma in situ elements. Immunohistochemical studies showed evidence suggesting myoepithelial and epithelial differentiation as exhibited by immunoreactivity for at least one myoepithelial and epithelial markers in all pure spindle cell components. IDC group showed 21.7% of axillary lymph nodes metastasis rate, whereas the axillary lymph node metastasis rate of the SpCMC group was 1.3%, significantly lower than that of the IDC group (P < 0.001). Immunohistochemical staining of IDC exhibited higher degrees of positivity for ER, PR and Her2 (90, 60 and 30%, respectively) when compared with the SpCMC group, which showed a positive degree of 5.2, 5.2 and 10.5% for ER, PR and Her2, respectively (P < 0.001). Based on this series, SpCMC is a rare variant of metaplastic breast carcinoma with the distinct histopathological and immunohistochemical features. The biological behaviors of SpCMC, like axillary lymph node status, were quite different from that of IDC, suggesting that it may act as an independent pathologic subtype. Immunohistochemical analysis of a panel of epithelial and myoepithelial markers could contribute to the pathologic diagnosis of SpCMC. © 2014 Wiley Publishing Asia Pty Ltd.
    Asia-Pacific Journal of Clinical Oncology 12/2014; · 0.91 Impact Factor
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    ABSTRACT: To investigate the clinical features, therapeutic methods and therapeutic effects of sinonasal hemangiopericytoma.
    06/2014; 49(6):452-6.
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    ABSTRACT: To improve the accuracy of diagnosis and treatment efficacy of hemangiopericytoma in nasal cavity and paranasal sinuses.
    03/2014; 28(5):314-7.
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    ABSTRACT: To study the diagnosis and treatment for Langerhans cell histiocytosis (LCH) of head and face. Clinical data of 19 cases suffering from LCH of head and face verified by pathology was retrospectively analyzed. The clinical presentation, diagnosis and treatment were discussed. Of all the 19 cases, 5 patients had an involvement of the scalp or skull, 5 had the maxilla involvement and 9 temporal bone. Among the 19 patients, type I stage I was 8 cases(all adults), type II stage I 7 cases (all children), type ILL stage II 2 (1 adult, 1 child), stage III 1 and stage IV 1 (both child). Their main clinical manifestations included scalp mass, defect of skull, swelling or mass of facial maxilla and of ear or temporal bone, otorrhea, granuloma of auricular canal, and so on. Fifteen cases were cured by surgical curettage combined with radiotherapy and 4 by surgical curettage combined with chemotherapy. One patient had the sequelae of diabetes insipidus. Diagnosis of LCH should be based on synthetical analyses of clinical manifestations, features of imaging and histopathology. Surgical curettage, radiotherapy and chemotherapy are effective treatment regimens. The prognosis is generally good if the patient gets correct diagnosis and timely rational treatment.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 07/2013; 27(13):704-7.
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    ABSTRACT: This study is aimed at investigating whether or not human leukocyte antigen-G (HLA-G) expression is associated with breast cancer molecular subtypes. HLA-G expression was immunohistochemically investigated in 104 patients with invasive ductal breast carcinoma, in which 56 were luminal A, 17 were luminal B, 19 were HER-2, and 12 were basal-like/normal breast-like subtype classified according to immunohistochemical staining results of ER, HER-2, CK5/6, and EGFR. Host immune response status was assessed by estimating the density of tumor infiltrating lymphocytes (TIL). For comparison, other biomarkers such as Ki67, p53 and VEGF were also investigated. Associations of these biomarkers and TIL with molecular subtypes were statistically analyzed. We found that there were more cases with high expressions of HLA-G in non-luminal than in luminal subtypes (P=0.035). In contrast, more cases with high density of TIL was found in luminal than in non-luminal subtypes (P=0.023). Compared to all the biomarkers studied, only HLA-G expression was found to be inversely associated with the density of TIL (P=0.004). Furthermore, patients with HLA-G(high)/TIL(low) status had a higher risk of recurrence than those with HLA-G(low)/TIL(high) status, regardless of the molecular subtypes. Therefore, a combination of the status of HLA-G and TIL could improve the prognostic prediction for patients with various molecular subtypes of breast cancer.
    Human immunology 07/2012; 73(10):998-1004. · 2.55 Impact Factor
  • Lin Dai, Gang Xu
    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 09/2011; 32(9):642-4.
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    ABSTRACT: To study the roles of histologic examination and polymerase chain reaction in diagnosis of toxoplasmic lymphadenitis (TL). Forty-six archival cases of histologically diagnosed TL, encountered during the period from April, 1999 to September, 2009 and with the paraffin-embedded lymph node tissue blocks available, were enrolled into the study. The presence of genome fragments of Toxoplasma gondii (T. gondii) was analyzed using semi-nested polymerase chain reaction (PCR). Thirty cases of one or two histopathologic triad of TL as the controls. The positive rate of PCR in TL group was 76.1% (35/46), as compared to 10.0% (3/30) in the control group. The difference was of statistical significance. The sensitivity and specificity of the histologic triad in diagnosing TL was 92.1% (35/38) and 71.1% (27/38), respectively. The predictive value of positive and negative PCR results was 76.1% (35/46) and 90.0% (27/30). respectively. The high specificity but low sensitivity of applying the histologic triad in diagnosing TL cases may be due to the occurrence of atypical histologic pattern. The sensitivity is improved with the use of semi-nested PCR in detecting T. gondii DNA.
    Zhonghua bing li xue za zhi Chinese journal of pathology 06/2010; 39(6):361-5.
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    ABSTRACT: Human leukocyte antigen-G (HLA-G) expression in 55 cervical intraepithelial neoplasia (CIN) patients with or without human papillomavirus (HPV) infection and 116 patients with squamous cell cervical cancer were examined using immunohistochemistry. Host immune response was assessed by estimating the number of intratumoral lymphocyte infiltration (TIL) in all lesions and counting CD57-expressing cells in the neoplasm lesions. The means of HLA-G immunoreactive scores were compared by the Mann-Whitney test and 1-way analysis of variance (ANOVA). The association of HLA-G expression with disease progression, HPV infection and host immune response was calculated using the Pearson Chi-square test. It was found that HLA-G expression increasingly progressed from patients with CIN 1 to CIN 2/3 and was highest in patients with cervical cancer. Human leukocyte antigen-G expression was also significantly higher in CIN and cancer patients with HPV 16/18 than in CIN patients without HPV. A significant correlation between HLA-G expression and TIL score or the counting of CD57-expressing cells was also evident in CIN patients with HPV infection and cervical cancer cases. These results suggest that HLA-G expression in cervical lesions is associated with carcinogenesis, HPV infection, and host immune response.
    Reproductive sciences (Thousand Oaks, Calif.) 05/2010; 17(8):718-23. · 2.31 Impact Factor
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    ABSTRACT: To evaluate the diagnostic utility of Warthin-Starry silver stain, immunohistochemistry and transmission electron microscopy in the detection of human Bartonella henselae infection and pathologic diagnosis of cat scratch disease (CSD). The paraffin-embedded lymph node tissues of 77 histologically-defined cases of cat scratch disease collected during the period from January, 1998 to December, 2008 were retrieved and studied using Warthin-Starry silver stain (WS stain) and mouse monoclonal antibody against Bartonella henselae (BhmAB stain). Five cases rich in bacteria were selected for transmission electron microscopy. Under electron microscope, the organisms Bartonella henselae appeared polymorphic, round, elliptical, short rod or bacilliform shapes, ranged from 0.489 to 1.110 microm by 0.333 to 0.534 microm and often clustered together. Black short rod-shaped bacilli arranged in chains or clumps were demonstrated in 61.0% (47/77) of CSD by WS stain. The organisms were located outside the cells and lie mainly in the necrotic debris, especially near the nodal capsule. In 72.7% (56/77) of the cases, dot-like, granular as well as few linear positive signals were observed using BhmAB immunostain and showed similar localization. Positive results for both stains were identified in 59.7% (46/77) of the cases. When applying both stains together, Bartonella henselae was observed in 74.0% (57/77) of the case. The difference between the results obtained by WS stain and BhmAB immunostain was of statistical significance (P < 0.05). Bartonella henselae is the causative pathogen of cat scratch disease. WS stain, BhmAB immunostain and transmission electron microscopy are helpful in confirming the histologic diagnosis. Immunostaining using BhmAB can be a better alternative than WS stain in demonstrating the organisms.
    Zhonghua bing li xue za zhi Chinese journal of pathology 04/2010; 39(4):225-9.
  • Gang Xu, Huai-Fu Wang, Gang He
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    ABSTRACT: To investigate the significance of P53 and P21 expressions in nasal NK/T-cell lymphoma (NKTL) and their relationships with the clinical stage, prognosis, proliferation and apoptosis of tumor cells. The clinicopathologic and follow-up data was collected from 62 patients with NKTL proven by pathological examination. Sixty-two cases of NKTL were examined for P53, P21 and Ki67 proteins with tissue microarray technique and immunohistochemistry. TUNEL method was used to detect apoptosis (apoptosis index, AD). The proliferation index (PI) was determined by the expression of Ki67 proteins. The positive rates of P53 and P21 protein expression in NKTL were 79.03% and 58.06% respectively. The positive rates of P53 in Ann Arbor stage I, II, III and lV NKTL were 69.57%, 75.00%, 86.67% and 100.00% respectively, while those of P21 were 47.83%, 56.25%, 60.00% and 87.50%. With the progression of tumor, the positive expression rates of P53 and P21 proteins gradually increased. And there were significant differences statistically between the expressions of P53/ P21 and Ann Arbor stage (P < 0.05). The expression of P53 was positively correlated with the expression of P21(r = 0.467, P < 0.05). The prognosis of P53 and P21 positive expression group was worse than that of negative expression group (P < 0.05). The expression of P53 protein was an independent prognostic factor. The intensities of P53 and P21 expressions were positively correlated with PI (r = 0.177, 0.184, P < 0.05), while not correlated with AI (P > 0.05). The expressions of P53, P21 and Ki67 proteins are closely related with the pathogenesis and progression of NKTL. Combined detection of P53, P21 and Ki67 could be better to judge the biological behavior of NKTL.
    Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 01/2010; 41(1):132-6.
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    ABSTRACT: To investigate the expression of murine double minute 2 (mdm2), p53, p21 and latent membrane protein 1 (LMP-1) in nasal NK/T-cell lymphoma (NKTL) and analyze their relationship with the clinical stage and prognosis. The clinicopathological and follow-up data of 62 patients with NKTL proven by pathology were collected. Paraffin-embedded tissue sections were examined for mdm2, p53, p21 and LMP-1 proteins by tissue microarray technique and immunohistochemistry. In situ hybridization was used to detect EBER1/2. The positive expression rates of mdm2, p53, p21 and LMP-1 proteins in NKTL were 61.3%, 79.0%, 58.1% and 48.4%, respectively, and EBER1/2 was 90.3%. The positive expression rates of mdm2 in Ann Arbor stage I, II, III and IV NKTL were 43.5%, 62.5%, 73.3% and 87.5%, respectively; p53 were 69.6%, 75.0%, 86.7% and 100.0%; p21 were 47.8%, 56.3%, 60.0% and 87.5%; while those of LMP-1 were 60.9%, 50.0%, 26.7% and 50.0%, respectively. With the progression of tumor, the positive expression rates of mdm2, p53 and p21 proteins gradually increased. There were statistically significant differences between them (P<0.05). There were statistically positive correlations among those three genes (P<0.05). The prognosis of the positive expression group of these three genes was worse than that of negative expression group (P<0.05). No statistically significant difference was observed between the expression of LMP-1 protein and the clinical stage or prognosis (P>0.05). The expression of p53 protein was an independent prognostic factor. The expression of mdm2, p53 and p21 proteins is closely related with the pathogenesis and progression of NKTL. They are good markers for judgement of the biological behavior of NKTL. The expression of p53 protein is an independent prognostic factor. Though no significant relationship was found between the expression of LMP-1 protein and the clinical stage or prognosis, it may play some role in tumor progression.
    Zhonghua zhong liu za zhi [Chinese journal of oncology] 05/2009; 31(5):351-5.
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    ABSTRACT: To investigate the significance of expressions of p53 and p21 in nasal NK/T-cell lymphoma (NKTL) and their relationship with cell proliferation and apoptosis. Sixty-two cases of NKTL were examined for p53, p21 and Ki67 proteins by means of tissue microarray technique, TUNEL and immunohistochemistry. The proliferation index (PI) was determined by expression of Ki67 proteins. The positive expression rates of p53 and p21 proteins in NKTL were 79.03% and 58.06% respectively. The positive expression rates of p53 in Ann Arbor stage I, II, III and IV NKTL were 69.57%, 75%, 86.67% and 100% respectively, while those of p21 were 47.83%, 56.25%, 60% and 87.50%. With the progression of tumor, the positive expression rates of p53 and p21 proteins gradually increased. And there were significant differences between them (P<0.05). The positive expression rates of p53 in NKTL with large, medium and small size tumor cells were 92.86%, 78.95% and 53.33% respectively, while those of p21 were 67.86%, 57.89% and 40.00%. With the expanding of tumor cells, the positive expression rates of p53 and p21 proteins gradually increased. And there were significant differences between them (P<0.05). The expression of p53 was positively correlated with the expression of p21 (P<0.05). The intensity of p53 and p21 expression, the Ann Arbor stage and the size of tumor cell all were positively correlated with PI (Spearman correlation analysis, P<0.05), while no correlation with AI (P>0.05). The expressions of p53, p21 and Ki67 proteins are closely related with the pathogenesis and progression of NKTL. Combined detection of p53, p21 and Ki67 is a good marker to judge the biological behavior of NKTL, such as the proliferation and the invasiveness of the tumor.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 02/2009; 23(2):73-6.
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    ABSTRACT: To study the extent of distal intramural spread of distal 2 cm rectal carcinoma over the dentate line, and to improve quality of life for these patients through providing pathological evidence of operation mode choice. Specimens of thirty patients with rectal carcinoma, operated with ISR(intersphincter resection) or Miles procedure from May 2005 to July 2007, were collected, and their large and histology pathologic slices were examined. Specimens were dissected distally to the dentate line per 2 mm within 1cm and per 5 mm exceed 1cm. The length of distal intramural spread to rectal carcinoma was measured under light microscope. Among the 30 specimens, distal intramural spread over the dentate line was observed only on one case and the invasion length over the dentate line was more than 2 cm, the invasion lengths of the other 29 cases were within the dentate lines. Distal 2 cm rectal carcinomas seldom spread over dentate lines. Anus discomfortableness of these patients and other complications could be reduced through reserving more skin over the dentate line, which are important for the improving of quality of life in these patient.
    Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery 02/2008; 11(1):44-6.
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    ABSTRACT: To investigate the clinicopathological features and prognostic factors of nasal NK/T-cell lymphoma. The clinicopathological data, treatment modality and survival status were analyzed. The overall five year survival rate of: 62 patients was 63.5%. In univariate analysis, the prognosis of the patients was correlated with the age, perforation, B symptom, stage, Hb concentration, LDH level, size of tumor cell, number of involvement sites, angioinvasion and treatment modality (P < 0.05 or P <0.01). In multivariate analysis, the Hb concentration, the size of the tumor cell and the treatment modality were the independent prognostic factors. The Hb concentration, the size of the tumor cell and the treatment modality play key role in the prognosis of nasal NK/T-cell lymphoma.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 10/2007; 21(20):932-4.