Genetic

Publications (2)2.13 Total impact

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  Available from: Emmanuel Nnaemeka Nnadi

  Article: GENOTYPING AND FLUCONAZOLE SUSCPTIBILITY OF Candida albicans STRAINS FROM PATIENTS WITH VULVOVAGINAL CANDIDIADIS IN JOS, NIGERIA

  Nnadi Nnaemeka Emmanuel, Orazio Romeo, Ayanbimpe Grace Mebi, Ojogba Okolo Mark, Enweani Ifeoma Bessie4 Fabio Scordino, Giuseppe Criseo2 1. Department of Medical Microbiology, University of Jos, Jos. Plateau State 2. Department of Life Sciences ‘‘Marcello Malpighi’’, Section of Microbiological, Genetic, [......], University of Messina, Messina, Italy 3. Department of Medical Microbiology, Jos University Teaching Hospital, Jos, Plateau State, Nigeria 4. Faculty of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi. Anambra State
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  ABSTRACT: ABSTRACT Objective To investigate the fluconazole susceptibility of the genotypes of Candida albicans strains isolated from patients with Vulvovaginal Candidiasis (VVC) in Jos, Plateau State – Nigeria. Method A total of one hundred and seventy seven (177) Candida isolates were examined. The strains were obtained from 320 female patients with VVC and were identified using both phenotypic and molecular methods. Their genotypes were determined, based on the presence or absence of a transposable intron in the 25S rDNA. Result Eighty four (84) strains were recognized as being C. albicans and all the 84 C. albicans strains resulted to be genotype A and antifungal susceptibility testing showed that 13 of those isolates (15.48%) were resistant to fluconazole. Conclusion Based on these data, we conclude that C. albicans genotype A is common among VVC patients in Jos, and resistance to fluconazole is quite high. To our knowledge, this is the first study that reports C. albicans genotypes in Jos and its resistance to fluconazole
  Asian Pacific Journal of Tropical Disease. 02/2012; 2(1):48–50.
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  Available from: Giuseppe Criseo

  Article: Isolation and molecular characterization of Candida africana from Jos, Nigeria.

  Nnaemeka Emmanuel Nnadi, Grace Mebi Ayanbimpe, Fabio Scordino, Mark Ojogba Okolo, Ifeoma Bessy Enweani, Giuseppe Criseo, Orazio Romeo2 1 Department of Medical Microbiology, University of Jos, P.M.B, Jos, Nigeria. 2 Department of Life Sciences “Marcello Malpighi” section of Microbiological, Genetic, Molecular Sciences, University of Messina, Messina, Italy. 3Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Nigeria
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  ABSTRACT: During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. rDNA-based genotyping, showed that two C. africana, including all C. albicans isolates examined belonged to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.
  Medical mycology: official publication of the International Society for Human and Animal Mycology 01/2012; · 2.13 Impact Factor