Fang Wang

Publications (5)1.55 Total impact

• Source

  Available from: Qiao Niu

  Article: Effects of benzo[a]pyrene on autonomic nervous system of coke oven workers.

  Hong-Mei Zhang, Ji-Sheng Nie, Fang Wang, Ying-Tao Shi, Ling Zhang, Andrea Antonucci, Hui-Jun Liu, Jing Wang, Jie Zhao, Qin-Li Zhang, Lin-Ping Wang, Jing Song, Cui-E Xue, Mario Di Gioacchino, Qiao Niu
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  ABSTRACT: Objectives are to investigate the effects of benzo[a]pyrene (B[a]P) on the autonomic nervous system of coke oven workers. One hundred eighty-four coke oven workers were divided into 3 groups according to their working sites (coke oven bottom group, coke oven side group and coke oven top group), and 93 referents were recruited. B[a]P monitored by air sampling pumps as well as urinary 1-hydroxypyrene (1-OH-Py) was determined by high performance liquid chromatograph with a fluorescence detector (HPLC-FD). The autonomic nervous system (ANS) function was determined by 4 tests: Valsalva Manoeuvre heart rate variation (HR-V), variation of heart rate when breathing deeply (HR-DB), variation of heart rate when instantly standing up (HR-IS, including RR30:15 and RRmax:min) and variation of blood pressure when instantly standing up (BP-IS). The B[a]P mean concentrations in coke oven bottom, coke oven side and coke oven top were 19, 185 and 1,623 ng/m(3), respectively. The levels of urinary 1-OH-Py were markedly higher in the 3 exposed groups than that in the referent group (p<0.01). No significant difference was found in each group between smokers and non-smokers (p>0.05). Compared with referents, HR-V decreased significantly in coke oven workers (p<0.01), representing modulation of parasympathetic nervous function. However, no statistical differences were found in HR-DB, RR30:15, RRmax:min and BP-IS between the exposed groups and the control group (p>0.05). HR-V decreased with the increment of 1-OH-Py (p<0.05), and results of multiple linear stepwise regression demonstrated that external exposure level and duration of education entered the HR-V model; age was a significant factor of HR-DB and RRmax:min, but no variable was involved in RR30:15 and BP-IS regression. Benzo[a]pyrene affects the autonomic nervous function of coke oven workers mainly by down-regulating the parasympathetic nervous function.
  Journal of Occupational Health 05/2008; 50(4):308-16. · 1.55 Impact Factor
• Article: [Lipid peroxidation injury and endoplasmic reticulum stress in Al-induced apoptosis].

  Qin-li Zhang, Fang Wang, Ying-tao Shi, Lin-ping Wang, Ling Zhang, Hong-mei Zhang, Jing Wang, Qiu-ying Li, Qiao Niu
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  ABSTRACT: To study the role of lipid peroxidation injury and endoplasmic reticulum stress in Al-induced apoptosis. Neurons from 0-3 day rats were cultured and treated with different concentrations of AlCl3.6H2O. Morphologic changes of neurons and endoplasmic reticulum were observed under fluorescent and transmission electron microscope; activities of superoxide dismutase (SOD), malondialdehyde (MDA) and ATP enzymes were detected. Typical morphologic changes in neurons apoptosis and endoplasmic reticulum were found under fluorescent and transmission electron microscope; SOD enzyme viability and ATP enzyme viability were significantly increased in the low-dosage group, but reduced in mid and high-dosage group (P < 0.01), whereas MDA levels decreased in the low-dosage group, but increased in mid and high-dosage group (P < 0.01). Aluminum may induce neurons apoptosis, and lipid peroxidation injury in endoplasmic reticulum plays an important role in the apoptosis progression.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 03/2008; 26(3):143-6.
• Article: [Characteristic of the neurobehavioral functional changes in coke oven workers].

  Ji-Sheng Nie, Hong-Mei Zhang, Jian-Ya Sun, Ying-Tao Shi, Fang Wang, Ping Zeng, Lin-Ping Wang, Jing Song, Jing Wang, Hui-Jun Liu, Qiao Niu
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  ABSTRACT: To explore the effect of polycyclic aromatic hydrocarbons on the neurobehavioral function of coke oven workers. 200 healthy adult male coke oven workers were selected from a coke plant of a state-owned steel enterprise in Taiyuan City. 88 controls occupationally unexposed to polycyclic aromatic hydrocarbons (PAHs) were selected from the same enterprise. All the subjects participated in this investigation voluntarily in their consent. Concentration of B(a)P in the working environment was monitored by High Performance Liquid Chromatography (HPLC). Urine samples were sampled immediately after working shifts. The level of urinary 1-hydroxypyrene was determined by HPLC. General information of workers correlated with the investigation was collected in a questionnaire according to the same criteria by well-trained investigators. Neurobehavioral core test battery (NCTB) recommended WHO was performed on coke oven workers and controls to test the neurobehavioral changes and the mood state. the concentration of B(a)P at oven bottom,oven side and oven top were 0.0195 microg/m3, 0.186 microg/m3 and 1.624 microg/m3 respectively, that at oven side and oven top being higher than the one stipulated by the occupational hygiene criterion. Urinary 1-hydroxypyrene was significantly different between the exposure group (3.42 +/- 0.98 micromol/mol creatinine) and control group (2.75 +/- 1.09 micromol/mol creatinine). No significant differences were found between exposure group and control group of age, working years, smoking, drinking and unhealthy food consumption; however, compared to the controls, the scores of total digital span, the forward digital span, and right dotting in the coke oven workers were lower, but that of total dotting was higher, with a statistical significance. According to urinary 1-hydroxypyrene concentration, all the subjects were divided into three groups. (<3.10 micromol/mol creatinine, 3.10 micromol/mol creatinine, >3.87 micromol/mol creatinine). Significant differences of the total digital span, the forward digital span, backward digital span, digit symbol and Benton visual retentions existed in different urinary 1-hydroxypyrene concentration groups and showed a dose-response tendency. Results of multiple stepwise regression analysis and correlation analysis showed that the level of urinary 1-hydroxypyrene affected memory and perception of coke oven workers and negative correlations between the level of urinary 1-hydroxypyrene and changes in neurobehavioral function were found. PAHs mainly causes decrease of memory and perception in coke oven workers.
  Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 02/2008; 42(1):25-9.
• Article: [Relationship between lipid peroxidation in blood and neurobehavioral function changes in coke oven workers].

  Fang Wang, Hong-Mei Zhang, Ji-Sheng Nie, Cui-E Xue, Ying-Tao Shi, Qiao Niu
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  ABSTRACT: To explore the coincidence of lipid peroxidation and neurobehavioral function changes in coke oven workers. One hundred and thirty-four coke oven workers were divided into three groups: 35 in the oven-bottom group, 49 in the oven-side group and 50 in oven-top group. WHO recommended NCTB was performed on coke oven workers and 36 controls from material conservation department; The contents of total superoxidedismutases (T-SOD), glutathione (GSH) and malondialdehyde (MDA) in blood were determined by test kits. Compared with the controls, the coke oven workers showed lower levels of T-SOD and GSH (P < 0.01), significantly higher MDA levels in blood (P < 0.01), higher score on negative mood state, lower scores on positive mood state, and poorer performance in NCTB test (P < 0.05). Further analysis revealed that there was a weak positive correlation between neurobehavioral function changes and the level of lipid peroxidation with a coefficient lower than 0.25. The level of lipid peroxidation in coke oven workers' blood increased and coincided with neurobehavioral function impairment.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 02/2007; 25(1):15-7.
• Article: [Role of Bcl-2 and Bax protein contents and their gene expression in Al-induced neurons apoptosis].

  Qin-li Zhang, Pi-ye Niu, Ying-tao Shi, Hong-mei Zhang, Fang Wang, Ling Zhang, Qiao Niu
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  ABSTRACT: To study the role of Bcl-2 and Bax protein contents and their gene expression in Al-induced neurons apoptosis. Neurons from 0 - 3 day rats were cultured and treated with different concentrations of AlCl(3 x 6) H2O. The cell apoptosis was observed by the TUNEL method and under the scan electron microscope. Bcl-2 and Bax protein contents were detected by the immunochemistry method while their gene expressions were measured by the RT-PCR method. (1) DNA fractions in the TUNEL method increased with the rising aluminum concentration. Blebbings and apoptosis bodies on the surface of the neurons were clearly observed under the scan electron microscope. (2) Bcl-2 protein contents and their gene expression decreased with the rising aluminum concentration (P < 0.01, r = -0.695; P < 0.05, r = -0.647), while Bax increased at the same time (P < 0.01, r = 0.676; P < 0.01, r = 0.794), the value of Bcl-2/Bax was related with the aluminum concentration (P < 0.01, r = -0.655; P < 0.01, r = -0.777). The aluminum may induce neurons apoptosis. Bcl-2 and Bax protein contents and their gene expression may play an important role in Al-induced apoptosis.
  Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 11/2006; 24(10):582-6.