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ABSTRACT: VALIDATION OF GC-MS/MS METHOD FOR MULTI RESIDUE ANALYSIS OF PESTICIDES IN MANGO
Deepa G.T., Mahadev B. Chetti*, M.C.Khetagoudar, P.T.Goroji., Dinesh B.K.
*Corresponding author : Dean (Agri.), University of Agricultural Sciences,
Dharwad – 580 005, Karnataka, India,
Cell : 094484 95309, Fax : +91-836-2792180, E-mail address : mbchetti_uas@rediffmail.com
ABSTRACT
A gas chromatographic –tandem quadruple mass spectrometric [GC-MS/MS] method for analysis of multi class [Organochlorines, Organophosphorus, Pyrethroids and Triazoles] pesticide residues of mango has been developed and validated. Mass spectrometry is a very sensitive, selective technology for both multi residue and trace level identification of wide range of pesticides. The extraction of pesticide residues was carried out by dispersive solid phase extraction (dSPE) method, is based on the solvent extraction of homogenized mango sample by acetonitrile added to the 50 ml tube containing sodium acetate and magnesium sulphate. Co-extractives were removed with primary secondary amine (PSA) and graphitized carbon black (GCB), the samples were further cleaned up by using 0.2 μm filter paper. Identification of the compound done by matching the ful-scan spectra with the corresponding NIST library with the match threshold of > 80%. Confirmation criteria of the pesticides were based in the 2002/657/EC European Commission Decision. The method was validated by fortified mango samples at different concentration levels. Average recoveries ranged from 80-100% with Relative standard deviation [RSD] between 3.9% and 13.2%. Performance characteristics linearity, detection limit [LOD], quantification limit [LOQ], precision and recoveries were studied.
icas-2010: International Congress on Analytical Sciences 2010,, IMA house, Kochi- Kerala.; 01/2010
