Dong-Hee Kim

Daejeon University, Daiden, Daejeon, South Korea

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Publications (29)49.39 Total impact

  • Boo-Yong Sim · Hak-Joo Choi · Ji-Won Bak · Dong-Hee Kim
    11/2014; 29(6):95-102. DOI:10.6116/kjh.2014.29.6.95.
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    ABSTRACT: Silver-coated γ-Fe2O3 such as Ag/γ-Fe2O3 and, were synthesized and characterized for their anticancer activities. The Ag/γ-Fe2O3 nanoparticles sizes are ranges from 34 to 46 nm. The characteristics of these particles were determined via X-ray diffraction, field emission scanning electron microscopy, and Raman spectrometer. The nanocomposites were prepared by the in situ chemical reduction of silver ions in the presence of oleic acid. A magnetic phase, along with the molecules of polyethylene glycol, served as the spacer between the iron oxide and the silver nanoparticles. The results demonstrated that the Ag/γ-Fe2O3 nanoparticles were spherical. A cell viability assay revealed that the Ag/γ-Fe2O3 nanoparticles could inhibit the proliferation of cancer cells in a dose-dependent manner. In contrast, the magnetic nanoparticles showed a non-toxic activity against normal and cancer cells. More specifically, the 70% Ag-coated γ-Fe2O3 nanoparticles were 52.16% cytotoxic to the DU145 prostate cancer cells, 50% cytotoxic to the HeLa cervical cancer cells, 67% cytotoxic to the MCF-7 breast cancer cells, 57% cytotoxic to the U87 glioblastoma cancer cells, and 63.7% cytotoxic to the HS68 normal cells. Our results suggest that the Ag/γ-Fe2O3 nanocomposites could find applications as versatile anti-cancer agents.
    Science of Advanced Materials 11/2014; 6(11). DOI:10.1166/sam.2014.2191 · 2.91 Impact Factor
  • 07/2014; 29(4):69-76. DOI:10.6116/kjh.2014.29.4.69
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    ABSTRACT: In this study, the anti-tumor activity of mitoxantrone loaded on magnetic nanoparticles (MTMP) was examined using DU145 prostate cancer cells. Composite nanoparticles with an average size of 20 nm were prepared using a chemical co-precipitation technique. The MTMP nanoparticles were cytotoxic to DU145 cells and inhibited cell proliferation. The expression levels of apoptosis related proteins in DU145 cells, including PARP and caspase 3, were increased after MTMP treatment. In this study, the therapeutic potential of MTMP in targeted-therapy against prostate cancer was demonstrated and MTMP was more effective when coupled to drug delivery vehicle than pure mitoxantrone.
    Journal of Biomedical Nanotechnology 06/2013; 9(6):1071-5. DOI:10.1166/jbn.2013.1530 · 5.39 Impact Factor
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    ABSTRACT: Chinese quince (Chaenomeles sinensis; CS) is known as a traditional oriental medicine for treating anaphylaxis and viral infection. Mast cells play an important role in a variety of inflammatory diseases. The inhibitory effects of CS extract on the inflammatory response of human mast cells were examined. CS extract inhibited HMC-1 cell migration in response to stem cell factor (SCF). Its mechanism is involved in the inhibition of a surface expression of c-kit binding to SCF. Tumor necrosis factor (TNF)-α expression is induced by phorbol 12-myristate 13-acetate (PMA) and calcium ionophore (CaI). CS extract inhibited the TNF-α expression by blocking the activation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase (MAPK), and c-Jun-N-terminal kinase (JNK) in HMC-1 cells. CS extract suppressed the expression of interlukin (IL)-6, IL-8, and MCP-1 in human monocytic THP-1 cells, as well as the secretion of IL-6 in human keratinocytic HaCaT cells.
    Food science and biotechnology 04/2013; 22(2). DOI:10.1007/s10068-013-0107-8 · 0.66 Impact Factor
  • In Sik Kim · Dong-Hee Kim · Chi-Young Yun · Ji-Sook Lee
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    ABSTRACT: (S)-(+)-decursin is a biological coumarin compound isolated from Angelica gigas Nakai. (S)-(+)-decursin and its analogue have a variety of pharmacological activities. In the present study, the anti-inflammatory effect of a (S)-(+)-decursin derivative, (S)-(+)-3-(3,4-dihydroxy-phenyl)-acrylic acid 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano [3,2-g]-chromen-3-yl-ester (Compound 6, C6), on in vitro and in vivo atopic dermatitis was investigated. C6 suppressed the secretion of IL-6, IL-8, and monocyte chemotactic protein-1 increase by the house dust mite extract in the eosinophilic leukemia cell line and THP-1 cells. C6 inhibited the production of TARC, IL-6, and IL-8 increase by IFN-γ and TNF-α in the human keratinocyte cell line. In the in vivo experiment, NC/Nga mice were sensitized to 2,4-dinitrochlorobenzene, and then C6 or dexamethasone (Dex) were orally and dorsally administered for three weeks. C6 treatment reduced the skin severity score compared with that of the control group. C6 inhibited the thickening of the epidermis and inflammatory cell infiltration into the dermis by evaluating the histological examination. The serum immunoglobulin E (IgE) level decreased in the C6-treated group compared with that of the control group. The inhibitory effect of C6 on IgE concentration was similar to that of Dex. The levels of IL-4, IL-5, IL-13, and eotaxin increased after treatment with concanavalin A in mouse splenocytes. The cytokine levels of the C6-treated group were lower than those of the control group. Taken together, C6 may attenuate atopic dermatitis-like lesions through its anti-inflammatory effect, such as inhibition of IgE and inflammatory cytokines, and it may be valuable as a therapeutic drug for the treatment of atopic dermatitis.
    Molecular Biology Reports 01/2013; 40(3). DOI:10.1007/s11033-012-2339-8 · 1.96 Impact Factor
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    ABSTRACT: Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). Results: IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF– α) stimulated by LPS. Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.
    Journal of Acupuncture and Meridian Studies 08/2012; 5(4):191–192. DOI:10.1016/j.jams.2012.05.011
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    ABSTRACT: In this study, we synthesized a novel chemical, (E)-2-(3,4-dimethoxyphenyl)-4-oxo-4H-chromen-7-yl-3-(3,4-dimethoxyphenyl) acrylate (CSH) and investigated the effect of CSH on atopic dermatitis (AD) by evaluating the anti-inflammatory effect of CSH on immune cells in vitro and on atopic dermatitis-like lesions in vivo. Human monocytic THP-1 cells and human eosinophilic EoL-1 cells were treated with house dust mite extract in the absence and presence of CSH. Nc/Nga mice were sensitized to 2,4-dinitrochlorobenzne (DNCB) for 5weeks and then orally and dorsally administered with CSH or dexamethasone for 3weeks. CSH inhibited the secretion of monocyte chemotactic protein-1 (MCP-1), interleukin (IL)-6 and IL-8 due to house dust mite extract in THP-1 cells. CSH also suppressed the secretion of MCP-1 and IL-8 in EoL-1 cells. In vivo, the skin severity score decreased after CSH treatment as compared to the control group. CSH suppressed the inflammatory cell infiltration into the dermis and thickened the epidermis. CSH reduced serum IgE level as compared to the control group. The levels of IL-4, IL-5, IL-13 and eotaxin in mouse splenocytes increased after treatment with concanavalin A and the increase of the cytokines was decreased by pre-treatment with CSH. The inhibitory effects of CSH on atopic lesions of DNCB-treated Nc/Nga mice were similar to those of dexamethasone, despite differing degrees depending on results evaluated in this study. These results may contribute to the development of a therapeutic drug for the treatment of AD.
    Life sciences 07/2012; 91(9-10):338-44. DOI:10.1016/j.lfs.2012.07.021 · 2.30 Impact Factor
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    ABSTRACT: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- α) stimulated by LPS. IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.
    Journal of Acupuncture and Meridian Studies 06/2012; 5(3):139. DOI:10.1016/j.jams.2012.04.005
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    ABSTRACT: In this study, we investigated the effects of CCL2 on constitutive apoptosis of normal and asthmatic neutrophils. CCL2 blocked the constitutive apoptosis of normal neutrophils through CCR2. CCL2 also induced elevation of the cytosolic Ca(2+) concentration but had no effect on normal neutrophil chemotaxis. Constitutive apoptosis, calcium influx, and cell migration of asthmatic neutrophils were not affected by CCL2 stimulation. Supernatant collected from CCL2-treated normal neutrophils inhibited the constitutive apoptosis of normal neutrophils. Anti-apoptotic signaling mediated by CCL2 was found to be associated with the PI3K/Akt/ERK/NF-κB cascade in normal neutrophils. Both the cleavage of procaspase 3 and procaspase 9 and the decrease of in Mcl-1 expression were delayed by CCL2 stimulation. Inhibition of NF-κB blocked constitutive apoptosis of neutrophils from asthmatic patients via inhibition of the cleavage of procaspase 3 and procaspase 9, in contrast to normal neutrophils. NF-κB was involved in CCL2-induced anti-apoptotic signaling in normal neutrophils, whereas NF-κB functioned as a basal pro-apoptotic factor in asthmatic neutrophils. A better understanding of the difference in the regulation of neutrophil apoptosis due to CCL2 between normal individuals and asthmatics will enable elucidation of the role of CC chemokine in neutrophils and a framework for understanding the pathogenesis of asthma.
    Journal of Cellular Physiology 06/2012; 227(6):2567-77. DOI:10.1002/jcp.22995 · 3.87 Impact Factor
  • American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California; 05/2012
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    ABSTRACT: Duchesnea chrysantha belongs to the Rosaceae family and has been used traditionally for the treatment of various diseases in Korea and other parts of East Asia. This study examined the antiinflammatory effect of Duchesnea chrysantha extract (DcE) on atopic dermatitis in vitro and in vivo. DcE inhibited the production of IL-6, IL-8 and MCP-1 in THP-1 cells and the release of IL-6 and MCP-1 in EoL-1 cells after treatment with house dust mite extract. In the in vivo experiment, Nc/Nga mice were sensitized to DNCB and then orally and dorsally administered DcE (50 mg/kg in PBS) for 3 weeks. The DcE administration significantly reduced the skin severity score when compared with the control group and inhibited the thickening of the epidermis and infiltration of inflammatory cells into the dermis. In addition, the serum IgE levels decreased markedly in the DcE-treated mice when compared with the control group. The synthesis of IL-5, IL-13, MCP-1 and eotaxin was also decreased in splenocytes of the DcE-treated group, while IFN-γ was increased in the Dc-administered group. These results may indicate that DcE attenuates the development of atopic dermatitis-like lesions by lowering the IgE and inflammatory cytokine levels, and that it is useful in drug development for the treatment of atopic dermatitis.
    Phytotherapy Research 02/2012; 26(2):284-90. DOI:10.1002/ptr.3545 · 2.40 Impact Factor
  • Kwon-Jai Lee · Jeung-Hee An · Jae-Soo Shin · Dong-Hee Kim
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    ABSTRACT: This study examined the optical characteristics of bicalutamide-loaded magnetic/ethylene glycol composite nanoparticles (BMP), as well as their anti-cancer activity against cancer cells. The gamma-Fe2O3 magnetic nanoparticles (MNPs), approximately 20 nm in diameter, were prepared via a chemical co-precipitation method and coated with two surfactants to yield a water-based product. The characteristics of the particles were determined via X-ray diffraction (XRD), field emission scanning electron microscopy, and Raman spectrophotometry. The Raman spectra of the BMP showed peaks at 222, 283, 395, 520, 669 and 1316 cm(-1), with broadened band in comparison to the Raman spectra of the magnetic nanoparticles. The BMP absorbance evidenced a rapid increase, with a broad peak at 409 nm, thus reflecting a good loading of the bicalutamide onto the magnetic nanoparticles. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the MNPs were non-toxic against human brain cancer cells (SH-SY5Y), human cervical cancer cells (Hela), human liver cancer cells (HepG2), breast cancer cells (MCF-7), colon cancer cells (CaCO2) and human prostate cancers (Du 145, PC3) tested herein. In particular, BMPs were cytotoxic at 56% against DU145 cells, at 74.37% in SH-SY5Y cells, and at 58% in Hela cells. Our results demonstrated the biological applicability of BMP nanoparticles as anticancer agents and as agents for enhanced drug delivery against human prostate cancer cells. Our results indicated that the MNPs were biostable and that the BMP functioned effectively as drug delivery vehicles.
    Journal of Nanoscience and Nanotechnology 02/2012; 12(2):1611-5. DOI:10.1166/jnn.2012.4632 · 1.34 Impact Factor
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    ABSTRACT: In the present study, we investigated whether the Lagerstroemia indica Linn (LI) extract has an anti-inflammatory effect on lung inflammation in ovalbumin-induced asthmatic mice. The LI extract was obtained from dried and powdered whole plants of LI using 80% ethanol. ELISA was performed to evaluate cytokine concentration. BALB/c mice were used as a mouse model of asthma after asthmatic induction by ovalbumin sensitization and inhalation. We examined the effects of the LI extract on leukocyte infiltration and mucus secretion using cell count and histological stain. The amount of cytokines, such as interleukin (IL)-2, IL-4, IL-5, IL-13, and TNF-α, was increased in Jurkat cells using the extract from house dust mites. Increased cytokine concentrations were inhibited by the LI extract. The LI extract suppressed the increased expression of IL-6 after treatment with mite extract of EoL-1 cells and THP-1 cells. In an in vivo experiment using asthmatic mice, the LI extract significantly inhibited leukocytosis and eosinophilia in bronchoalveolar lavage (BAL) fluid and lung tissue samples. The LI extract inhibited the increase in mucus secretion by goblet cells, blocked the production of reactive oxygen species in BAL fluid cells, and blocked the protein expression of IL-5 in BAL fluid. The concentration of ovalbumin-specific IgE in BAL fluid was weakly inhibited by the LI extract. These results suggest that the LI extract may be used as a valuable agent for treating allergic diseases such as asthma due to its anti-inflammatory property.
    Journal of ethnopharmacology 07/2011; 136(3):422-7. DOI:10.1016/j.jep.2010.05.066 · 2.94 Impact Factor
  • American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado; 05/2011
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    ABSTRACT: This study examined the cytotoxic effects of the γ-Fe2O3 magnetic nanoparticles (GMNs) on a range of tumor cell lines. GMNs, approximately 20 nm in diameter, were prepared using a chemical coprecipitation technique, and coated with two surfactants to obtain a water-based product. A 3-(4, 5-dimethylthiazol-2-yl) −2, 5-diphenyltetrazolium bromide assay revealed the GMNs to be non-toxic to human fibroblast cells and most of the tumor cell lines tested. However, the magnetic nanoparticles coated with the anti-cancer drugs exhibited cytotoxic activity against tumor cells. These results suggest that GMNs are not cytotoxic to the tumor cells and normal human cells tested in this study. In addition, the magnetic nanoparticles are biostable and magnetic nanoparticles coated with anti-cancer drugs are effective drug delivery vehicles. These results highlight the potential of drug-coated Fe2O3 magnetic nanoparticles as new anti-tumor agents.
    Current Applied Physics 05/2011; 11(3):467-471. DOI:10.1016/j.cap.2010.08.022 · 2.03 Impact Factor
  • Journal of the Korean Society of Food Science and Nutrition 12/2010; 39(12):1800-1806. DOI:10.3746/jkfn.2010.39.12.1800
  • Ji-Sook Lee · Eun Ju Yang · Chi-Young Yun · Dong-Hee Kim · In Sik Kim
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    ABSTRACT: Asthma is a disease marked by airway inflammation. Petasites japonicus (Pj) is known as an herb for treating asthma, oxidant stress and gastric ulcer in traditional Oriental medicine. In this study, the inhibitory effects of Pj extract on asthmatic responses were examined both in vitro and in vivo. The Pj extract was acquired from whole plants of Petasites japonicus using 80% ethanol. Cytotoxicity of the Pj extract on Jurkat cells and THP-1 cells was determined using MTT assay. ELISA was performed to determine the expression levels of cytokines, chemokines, and IgE. BALB/c mice were used for an OVA-induced asthmatic mouse model. Reactive oxygen species (ROS) production was stained with 2',7'-dichlorofluorescein diacetate and measured by fluorescence-activated cell sorting analysis. The effects of the Pj extract on leukocyte infiltration and mucus production were determined using periodic acid-Schiff staining as well as hematoxylin and eosin staining. The Pj extract inhibits the increased release of interleukin (IL)-2, IL-4, IL-5, IL-13, and TNF-α due to house dust mite in Jurkat cells and blocks IL-6 expression in THP-1 cells without cytotoxicity. In the asthmatic mouse model, the Pj extract inhibits eosinophil infiltration, mucus hypersecretion, and IL-5 level in bronchoalveolar lavage (BAL) fluid, and it has a scavenging effect on ROS production of cells in BAL fluid. The Pj extract has suppressive properties for the pathogenesis of airway inflammation and may be used as a potent agent for the treatment of asthma.
    Journal of ethnopharmacology 10/2010; 133(2):551-7. DOI:10.1016/j.jep.2010.10.038 · 2.94 Impact Factor
  • Kwon-Jai Lee · Jeung-Hee An · Jae-Soo Shin · Dong-Hee Kim
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    ABSTRACT: Prostate cancer is one of the most preventable cancers, yet effective therapeutics, especially targeted therapy, are still lacking. Saponin has been reported to possess various biological properties such as anti-cancer and anti-inflammatory activity. In this study, the anti-tumor activity of magnetic nanoparticles loaded with ethylene glycol and a saponin complex (ESMP) against DU145 prostate cancer cells was examined. Composite nanoparticles with an average size of 23 nm were prepared using a chemical co-precipitation technique. The ESMP were cytotoxic to DU145 cells and specifically inhibited cell proliferation. In contrast, the magnetic nanoparticles by themselves showed no significant cytotoxicity. The expression levels of the angiogenesis related proteins, including phospho-extracellular signal-regulated kinase, p38 and pAKT, decreased after ESMP treatment. This study highlights the therapeutic potential of using ESMP for targeted-therapy against prostate cancer.
    Journal of Nanoscience and Nanotechnology 10/2010; 10(10):6962-6. DOI:10.1166/jnn.2010.2985 · 1.34 Impact Factor
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    ABSTRACT: This study examined the biostability and drug delivery efficiency of g- magnetic nanoparticles (GMNs) by cytotoxicity tests using various tumor cell lines and normal cell lines. The GMNs, approximately 20 nm in diameter, were prepared using a chemical coprecipitation technique, and coated with two surfactants to obtain a water-based product. The particle size of the GMNs loaded on hangamdan drugs (HGMNs) measured 20-50 nm in diameter. The characteristics of the particles were examined by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-TEM) and Raman spectrometer. The Raman spectrum of the GMNs showed three broad bands at 274, 612 and . A 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay showed that the GMNs were non-toxic against human brain cancer cells (SH-SY5Y, T98), human cervical cancer cells (Hela, Siha), human liver cancer cells (HepG2), breast cancer cells (MCF-7), colon cancer cells (CaCO2), human neural stem cells (F3), adult mencenchymal stem cells (B10), human kidney stem cells (HEK293 cell), human prostate cancer (Du 145, PC3) and normal human fibroblasts (HS 68) tested. However, HGMNs were cytotoxic at 69.99% against the DU145 prostate cancer cell, and at 34.37% in the Hela cell. These results indicate that the GMNs were biostable and the HGMNs served as effective drug delivery vehicles.
    Korean Journal of Materials Research 03/2010; 20(3):132-136. DOI:10.3740/MRSK.2010.20.3.132