[Show abstract][Hide abstract] ABSTRACT: Introduction: The excess accumulation of free fluid in the peritoneal cavity is due to its
multitude causes. Certain cytological and biochemical markers are reported for the
differential diagnosis of ascites. In the present study, ascitic patients with liver cirrhosis
and ovarian carcinoma are investigated.
Aim: To study the concentrations of certain trace elements and ceruloplasmin levels in
ascitic fluid and in serum of patients with liver cirrhosis in comparison to ovarian cancer
Study Design: The study includes 170 patients with liver cirrhosis, 95 patients with
ovarian cancer and 100 serum controls.
Place and Duration of Study: The study is performed in the department of
Gastroenterology, Osmania General Hospital Hyderabad, A.P, INDIA, between
December 2011 to July 2013.
Methodology: The trace elements such as magnesium, copper, zinc, iron and
ceruloplasmin were investigated in 170 patients with liver cirrhosis (120 males, 50
females, 22-75 years; mean age 46.2±11.2) and 95 ovarian cancer patients (19-84
years; mean age 50.2±12.2). All the analysts were measured in serum and ascitic fluid
by using standard commercial kits. 100 serum samples from healthy controls were also
included in the study.
Results: The mean serum concentrations of copper and ceruloplasmin were significantly
increased in ovarian cancer patients when compared to that of liver cirrhosis and healthy
controls (P<. 001, P<. 001) respectively. The mean zinc levels in serum samples were
low in both the groups when compared to controls (P<. 001). The ascitic fluid zinc levels
in ovarian cancer were higher when compared to that of liver cirrhosis (P<. 001). Similar
results were noted in the serum iron levels in both the groups. The serum magnesium
levels in liver cirrhosis are comparable to that of controls but the levels of magnesium in
ovarian cancer ascitic patients are high when compared to that of controls.
Conclusion: Our results showed that there are differences in trace elements and
ceruloplasmin levels in liver cirrhosis and ovarian cancer patients in both serum and
ascitic fluid samples. The cutoff value of copper and ceruloplasmin in serum samples are
134.5 μg/dl and 43.5 mg/dl, while in ascitic fluid were 46.5 μg/dl and 21.0 mg/dl
respectively. As the diagnostic efficiency of copper and ceruloplasmin in serum and
ascitic fluid samples are 97% to 99% with highest sensitivity and specificity, analysis of
trace elements with ceruloplasmin in depth with their ratios may be helpful in
differentiating the cirrhotic and neoplastic illness.
British Journal of Medicine & Medical Research. 05/2014; 4(21):3902-3911.
[Show abstract][Hide abstract] ABSTRACT: Abstract
Early diagnosis of peritoneal metastases from cancer patients provides better management and treatment aspects. In the
present study biochemical investigations such as albumin, total proteins, lactate dehydrogenase and cholesterol in the
serum and ascitic fluid aspirates were done in 46 ovarian malignant (46 females mean age 49.47±12.64 yrs) and 70
non-malignant ascites patients (54 males and 16 females mean age 49.1±10.78 yrs).The results in the fluid showed that
lactate dehydrogenase, total proteins and cholesterol levels were drastically increased, while SAAG is reduced in
malignant ascites when compared to non-malignant ascitic patients . The present study thus emphasizes that SAAG is
still useful in the differential diagnosis of ascites.
Key Words: Ascites, Differential diagnosis, Malignancy, Biochemical markers.
International Journal of Pharmacy and Technology. 01/2013; 5(1):5136-5143.
[Show abstract][Hide abstract] ABSTRACT: The differential diagnosis of ascites is important and is cumbersome .The
present study was conducted to obtain the basic biochemical data in serum and
ascitic fluid of patients with non-malignant ascites; These parameters utilize it for
confirming the underlying causative factors for ascites.The parameters analyzed
were liver enzymes (SGPT, SGOT and ALP), Adenosine deaminase (ADA),
Creatinine, Amylase, Lactate dehydrogenase (LDH), Total proteins and albumin. A
total of 69 patients with non- malignant ascites (55 Males and 14 Females mean
age 49 + 12 years) were studied over a period of one year. Involvement of liver
disease was noted in 47 patients (68%), while the rest of the patients had
peritoneal tuberculosis ( 7 cases – 10% ), nephrotic syndrome ( 9 cases -13% )
and pancreatic ascites ( 6 cases -9%).Biochemical analysis of the results with
Lactate dehydrogenase, proteins and albumin data showed that SAAG (Serum
ascites albumin gradient) is still the best criteria for the differential diagnosis of
ascites.The present study emphasize the SAAG is useful marker for diagnosis of
different ascites , and it will useful for the evaluation and diagnosis of malignant
International Journal of Science Innovations and Discoveries. 02/2012; 2(1):72-76.
[Show abstract][Hide abstract] ABSTRACT: Gastric cancer is a multifactorial disease with the involvement of both genetic and environmental risk factors. Genetic variation in genes encoding cytokines and their receptors determine the intensity of the inflammatory response, which may contribute to individual differences in the outcome and severity of the disease. Transforming growth factor (TGF-β) signaling pathway plays an important role in the genesis and progression of tumors through regulating cell proliferation and differentiation. A hospital-based case-control study was conducted to investigate whether TGF-β1 -509 C/T polymorphism can modify the risk of gastric cancer. Seventy endoscopically and histopathologically confirmed gastric cancer patients and 100 age and sex-matched healthy controls were enrolled in the case-control study. TGF-β1 -509 C/T gene polymorphism was carried out by amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method followed by agarose gel electrophoresis. Statistical analysis was applied to test for the significance of the results. The distribution of TGF-β1 genotypes at -509 C/T were CC 37.14%, CT 50%, and TT 12.86% in gastric cancer patients and CC 52%, CT 42%, and TT 6% in control subjects. The allelic frequencies of C and T were 0.621 and 0.379 in gastric cancer patients and 0.73 and 0.27 in control subjects, respectively. Our study imply that T allele of TGF-β1 -509 C/T genotypes may be a risk factor of genetic susceptibility to gastric cancer in south Indian population.