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Molecular Carcinogenesis 04/2010; 49(4):410-28. · 3.16 Impact Factor
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Molecular Carcinogenesis 12/2008; 48(5):465-78. · 3.16 Impact Factor
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Molecular Carcinogenesis 08/2008; 47(7):554-71. · 3.16 Impact Factor
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Molecular Carcinogenesis 03/2008; 47(9):707-32. · 3.16 Impact Factor
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Molecular Carcinogenesis 07/2007; 46(6):415-35. · 3.16 Impact Factor
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Molecular Carcinogenesis 01/2006; 44(4):300-16. · 3.16 Impact Factor
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Molecular Carcinogenesis 04/2003; 36(3):101-14. · 3.16 Impact Factor
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Molecular Carcinogenesis 03/2002; 33(2):67-80. · 3.16 Impact Factor
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ABSTRACT: A method is described for the isolation of epithelial cells from normal human esophagus and the establishment of the epithelial cells in monolayer cell culture using irradiated 3T3 fibroblast feeder layers as a substrate. The monolayer cultures were subsequently used to study the effects of a tumor promoter and carcinogen on human esophageal epithelial cells in vitro.
Methods in Cell Science 01/1986; 10(4):227-232.
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ABSTRACT: Explants of adult human bronchus were cultured in CMRL-1066 medium supplemented with heat-inactivated fetal bovine serum,
hormones, antibiotics, and either 0.0, 0.1 1, or 10 mM putrescine. The outgrowth of bronchial epithelial cells was stimulated in medium containing 1 mM putrescine, a concentration that partially inhibited the outgrowth of fibroblasts. In medium containing 10 mM putrescine, the outgrowth of epithelial cells was similar to that observed in the control medium, but the outgrowth of fibroblasts
was completely inhibited for periods of at least 4 weeks. When 10 mM putrescine was added to cultures of bronchial fibroblasts, the fibroblasts were not killed. These results suggest that human
bronchial epithelial cells have a higher requirement for putrescine for growth than fibroblasts, and the molecular basis for
this observation is under investigation.
In Vitro Cellular & Developmental Biology - Plant 01/1980; 16(5):399-406. · 1.50 Impact Factor
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ABSTRACT: Epithelial cells derived from bovine pancreatic duct have been grown continuously in culture for 30 weeks (approximately 90
doublings of the cell population). The cells were grown in Eagle's minimal essential medium supplemented with 10% heat-inactivated
fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, and antibiotics. In confluent cultures, the cells are
multilayered and form circular structures. When tested at various passages, the cells neither formed colonies in soft agar
nor produced tumors after inoculation into athymic, nude mice. Hydrocortisone (1 and 5 μg per ml) and insulin (1,5 and 10
μg per ml) had no effect on the growth of the cells. β-Retinyl acetate inhibited growth rate and cell yield at a concentration
of 5 μg per ml but was not growth-inhibitory at lower concentrations. By electron microscopy the cells have numerous mitochondria,
Golgi and microvilli. Mucous droplets were observed in a small proportion of the cells. Desmosome-like structures and occluding
junctions were observed more frequently between cells that had been transferred as aggregates than between cells transferred
as single cells. Cytochemical studies indicated that some cells produce PAS positive granules that were not removed after
treatment of the cultures with diastase. Eleven cell clones were isolated from the mass culture. The growth rates of the clones
are different as well as the period of time in which the clones can be propagated in vitro.
In Vitro Cellular & Developmental Biology - Plant 04/1978; 14(7):581-590. · 1.50 Impact Factor
