C-T Yuen

National Institute for Biological Standards and Control, Potters Bar, England, United Kingdom

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Publications (1)1.95 Total impact

  • C-T Yuen, C K Gee, C Jones
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    ABSTRACT: Monitoring protein glycosylation is becoming increasingly important as novel recombinant glycoprotein therapeutics, such as glycoprotein hormones, cytokines and clotting factors, are introduced into clinical use. In this report, we describe an HPLC strategy and an improved and simplified pre-column derivatization procedure to profile N-linked glycans obtained from a variety of commercially available glycoproteins as examples. N-Glycans were first released by peptide:N-glycosidase F and labelled with the fluorescent label, 4-aminobenzoic acid by reductive amination. The labelled N-Glycans were then resolved by normal-phase HPLC and the N-glycan profile could be further improved by separating the N-glycans first according to charge by anion-exchange HPLC prior to the normal-phase HPLC. If required, identification of the fractionated derivatized oligosaccharides can be determined by mass spectrometry. The whole profiling process is simple and can be implemented in most laboratories. Because of the high sensitivity, batch glycan-analysis of low-yield recombinant glycoproteins such as samples in ampoules or obtained in the early stage of production development is possible.
    Biomedical Chromatography 07/2002; 16(4):247-54. · 1.95 Impact Factor

Publication Stats

19 Citations
1.95 Total Impact Points

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  • 2002
    • National Institute for Biological Standards and Control
      Potters Bar, England, United Kingdom