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ABSTRACT: Monoclonal antibodies against the corn stunt spiroplasma were produced by fusing NS-1/1-Ag4-1 murine myeloma cells with splenic cells from mice immunized with corn stunt spiroplasma (I-747). Isotypes of these monoclonal antibodies have been determined to be IgG1 or IgG2a subclass by the Ouchterlony double diffusion method using class and subclass specific antibodies for mouse IgG1, IgG2a, IgG2b, IgG3, and IgM. Antibody titers for both hybridoma culture supernatants and ascitic fluids were determined with indirect biotinylated enzyme-linked immunosorbent assay and they ranged from 800 to 3200, and 312 500 to 7 812 400, respectively. In serological specificity studies, with indirect enzyme-linked immunosorbent assay, 32 spiroplasma strains belonging to 19 different serogroups were tested using both corn stunt spiroplasma monoclonal antibodies and conventionally produced polyclonal antibodies. Corn stunt spiroplasma specific monoclonal antibodies reacted specifically with any of the three corn stunt spiroplasma strains tested, but not with all of the other spiroplasma strains from different groups and subgroups. On the other hand, some nonspecific reactions to heterologous spiroplasma strains were obtained when conventional polyclonal antibodies were used.
Canadian Journal of Microbiology 02/2011; 31(10):900-904. · 1.36 Impact Factor
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W L Tsai,
J S Cheng,
K H Lai, C P Lin,
G H Lo,
P I Hsu,
H C Yu,
C K Lin,
H H Chan,
W C Chen,
T A Chen,
W L Li,
H L Liang
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ABSTRACT: The long-term outcome of percutaneous acetic acid injection (PAI) and percutaneous ethanol injection (PEI) for treating small hepatocellular carcinoma (HCC) remains unclear.
To compare the long-term outcome of PAI vs. PEI for treating small HCC.
From July 1998 to July 2004, 125 patients with small HCC were enrolled. Seventy patients receiving PAI and 55 patients receiving PEI were enrolled. There were no significant differences in the clinical characteristics between the two groups. Tumour recurrence and survival rates were assessed.
Mean follow-up time was 43 months. The local recurrence rate and new tumour recurrence rate were similar between the PAI and PEI groups. The PAI group had significantly better survival than the PEI group (P = 0.027). Multivariate analysis revealed that PAI was the significant factor associated with overall survival [PAI vs. PEI, RR: 0.639, 95% CI: (0.419-1.975), P = 0.038]. The treatment sessions required to achieve complete tumour necrosis were significantly fewer in the PAI group than in the PEI group (2.4 +/- 1.0 vs. 2.9 +/- 1.3, P = 0.018).
Percutaneous acetic acid injection required fewer treatment sessions than PEI and provided better survival after long-term follow-up.
Alimentary Pharmacology & Therapeutics 09/2008; 28(3):304-11. · 3.77 Impact Factor
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W. L. Tsai,
J S Cheng,
K H Lai, C P Lin,
G H Lo,
P I Hsu,
H C Yu,
C K Lin,
H H Chan,
W C Chen,
T A Chen,
W L Li,
H L Liang
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ABSTRACT: Background: The long-term outcome of percutaneous acetic acid injection (PAI) and percutaneous ethanol injection (PEI) for treating small hepatocellular carcinoma (HCC) remains unclear Aim: To compare the long-term outcome of PAI versus PEI for treating small HCC. Methods: From July 1998 to July 2004, 125 patients with small HCC were enrolled. Seventy patients receiving PAI and 55 patients receiving PEI were enrolled. There were no significant difference in clinical characteristics between the two groups. Tumor recurrence and survival rates were assessed. Results: Mean follow-up time was 43 months. The local recurrence rate and new tumor recurrence rate were similar between the PAI and PEI group. The PAI group had significantly better survival than the PEI group (P=0.027). Multivariate analysis revealed PAI was the significant factor associated with overall survival (PAI vs PEI, RR: 0.639, 95% CI: (0.419-1.975), P=0.038). The treatment sessions required to achieve complete tumor necrosis were significantly fewer in the PAI group than the PEI group (2.4+/-1.0 vs 2.9+/-1.3, P=0.018) Conclusion: PAI required fewer treatment sessions than PEI and provided better survival after long-term follow-up.
Alimentary Pharmacology & Therapeutics 05/2008; · 3.77 Impact Factor
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P I Hsu,
K H Lai,
G H Lo,
C K Lin,
C C Lo,
E M Wang,
Y Y Wang,
W L Tsai, C P Lin,
H H Tseng,
H C Chen,
J L Chen
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ABSTRACT: Endoscopic ligation has been extensively applied in the management of esophageal and gastric varices with or without bleeding. The varices are automatically eradicated through the use of ligation. However, whether avascular necrosis will occur in a gastrointestinal polyp when the base is ligated remains unclear. The aims of this pilot study were to investigate the sequential changes of gastric hyperplastic polyps following endoscopic detachable snare ligation and to determine the possibility of induction of avascular necrosis in these lesions following ligation.
Eleven patients with eighteen gastric hyperplastic polyps were treated with endoscopic detachable-snare ligation. The polyps were observed for 5 minutes and biopsies were then conducted. At 14 days after endoscopic ligation, follow-up endoscopies were performed to assess the outcome of the strangulated polyps.
After being strangulated by the detachable snares, a majority of the polyps immediately congested (94%), and then developed cyanotic change (89%) approximately 4 minutes later. Pathological examination revealed severe venous congestion in the lamina propria of the strangulated polyps. On follow-up endoscopy 2 weeks later, all the snares had dropped off, and avascular necrosis occurred in sixteen polyps (89%). All of the polyps with avascular necrosis were detected to have developed cyanotic changes in initial endoscopy. No complications occurred during or following the ligation procedure.
Most gastric hyperplastic polyps develop avascular necrosis following ligation by detachable snare. Cyanotic change is an important predictor of the outcomes of the lesions following endoscopic ligation. The application of this ligation technique in treatment of bleeding or non-bleeding gastrointestinal polyps deserves further investigation.
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed 12/2001; 64(11):609-14.
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C C Lo,
P I Hsu,
G H Lo,
K H Lai,
J S Cheng,
H H Tseng,
C K Lin,
H H Chan,
Y Y Wang,
M K Ku, C P Lin,
N J Peng,
E J Chien
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ABSTRACT: The role of Helicobacter pylori (H. pylori) infection in non-ulcer dyspepsia (NUD) remains controversial. This study investigates the clinical, serological and histological differences between patients with H. pylori-positive and -negative NUD.
One hundred and eighty consecutive patients with NUD were enrolled from January to December 1998. The severity of symptoms was evaluated by the Tucci's scoring system. The histological changes of gastric mucosa were assessed according to the Updated Sydney System, and a fasting blood sample was obtained to test the serum gastrin and pepsinogen I levels.
The H. pylori-positive NUD patients were notably older than H. pylori-negative NUD patients (48.2 +/- 15.9 vs 39.8 +/- 15.7 years, P= 0.001). There were no differences in other clinical factors between the two NUD groups. The serum pepsinogen I levels were considerably higher in H. pylori-positive NUD patients than in H. pylori-negative NUD patients (78.9 +/- 42.2 vs 61.5 +/- 43.3 ng/mL, P<0.01). However, no significant differences in serum gastrin levels were discovered between the two groups. The antrum histological scores for chronic inflammation, acute inflammation, gland atrophy and lymphoid follicles were higher in H. pylori-positive NUD patients than in H. pylori-negative NUD patients (2.09 vs 1.01, P<0.001; 1.22 vs 0.36, P<0.001; 0.76 vs 0.36, P<0.01; 0.33 vs 0.13, P<0.01, respectively).
The present study discovered marked differences in age, serum pepsinogen I levels, histological grades of acute inflammation, chronic inflammation, gland atrophy and lymphoid tissue formation between H. pylori-positive and H. pylori-negative NUD patients. Further investigation of the clinical prognosis of the two groups of patients is necessary.
Journal of Gastroenterology and Hepatology 04/2001; 16(3):276-81. · 2.87 Impact Factor
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ABSTRACT: From October 1990 to January 1999, 11 of 2237 hepatoma patients at our hospital had gastrointestinal tract metastasis. We describe the radiological and endoscopic features, clinical course and prognoses of those patients.
All patients were male. Six patients were hepatitis B carriers, and cirrhosis was noted in eight patients on admission. We reviewed all available radiological, endoscopic and pathological features.
The commonest clinical presentation was frank gastrointestinal bleeding. Histological proof of gastrointestinal involvement was seen in six patients. Endoscopic features included ulcerative tumours mimicking advanced gastric carcinoma (43%) and submucosal tumours (29%). The sites of organ involvement were stomach (five), duodenum (two), colon (three) and duodenum and colon (one). Direct invasion by a contiguous neoplasm was the major route of gastrointestinal tract metastasis. Portal vein thrombosis may be the key point of haematogenous spread to other sites.
The prognosis in these patients was extremely poor. Almost all patients died within 5 months if no further aggressive management was performed. Surgical intervention may be the optimal choice for palliative treatment of HCC with gastrointestinal tract involvement.
Journal of Gastroenterology and Hepatology 06/2000; 15(5):536-41. · 2.87 Impact Factor
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ABSTRACT: Hereditary hemorrhagic telangiectasia, commonly known as Osler-Weber-Rendu disease, is a systemic autosomal dominant inherited disorder, that occurs in Caucasian populations. We report the case of a 56-year-old housewife who was admitted to the gastrointestinal and cardiovascular ward because she had suffered from recurrent gastrointestinal bleeding and heart failure from 1994 to 1997. Panendoscopy showed vascular ectasia scattered over the tongue, larynx, esophagus, and posterior wall of the gastric body. Colonoscopy showed clusters of telangiectasia over the cecum and ascending colon. Arteriovenous malformations (AVMs) were found in the liver and lungs on computerized tomography. Recurrent gastrointestinal bleeding was controlled by estrogen treatment during the follow-up period. In July 1997, the patient was readmitted to our cardiovascular section due to aggravated dyspnea, orthopnea and bilateral lower leg edema. Cardiac catheterization showed a large fistula from the left pulmonary artery to the left atrium and left ventricle, pulmonary arterial pressure of 37/13 mmHg and cardiac output of 9.61/minute. Other studies excluded the possibility of sepsis, and high-output cardiac failure was suspected. The patient was discharged in a stable condition and scheduled for AVM embolization management. Unfortunately, she died of a suspected heart attack at home two weeks following discharge.
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed 05/2000; 63(4):339-43.
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ABSTRACT: Hybridoma techniques have been applied to develop specific monoclonal antibodies (McAbs) against spiroplasmas and mycoplasma-like organisms (MLOs). Advantages of the McAbs have been clearly demonstrated in the detection of aster yellows (AY) MLO by indirect enzyme-linked immunosorbent assay (ELISA) and immunofluorescent staining, compared with conventionally produced polyclonal antibodies. McAbs have also been applied in the in vitro detection and identification of spiroplasmas and AY MLOs on membrane filters using modified direct-count immunofluorescent staining. Organisms were fixed with glutaraldehyde and treated with McAbs and fluorescein isothiocyanate (FITC)-conjugated antimouse immunoglobulin, by incubating and concentrating them in a microcentrifuge tube before collecting them on polycarbonate membrane. This procedure best conserves the helical integrity of spiroplasmas without altering the configuration of surface antigens recognized by McAbs. This method could be valuable in monitoring the fastidious mycoplasmas during purification or cultivation attempts.
Israel journal of medical sciences 07/1987; 23(6):763-6.
