Barbara Skop

Medical University of Silesia in Katowice, Catowice, Silesian Voivodeship, Poland

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Publications (9)17.29 Total impact

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    ABSTRACT: One of the important factors responsible for vessel wall remodelling is programmed cell death. In the paper the role of smooth muscle cell (SMC) apoptosis in primary varicose veins (PVV) is investigated. Vein specimens were obtained from 40 patients with PVV. In each case proximal and distal (upper crural) great saphenous veins (GSV) were harvested. Morphometric computer assessed quantitative evaluation of SMCs, collagen and elastin content was carried out. Apoptotic cells were detected by TUNEL assay. The levels of p53, BAX, BCLl-2 and p21 mRNA expression were assessed by real time RT-QPCR and the presence of respective proteins in the vessel wall was confirmed by immunohistochemistry. In the proximal GSV segments a significant increase of p53, p21 and BCL-2 mRNA levels was found in PVV patients. In the distal segments BAX and BCL-2 expression levels were higher. Taking into account the patient age, elevated p53 mRNA expression level was noticed in the distal incompetent GSVs of young PVV patients. In this group a statistically significant increase in the apoptotic index (APIx) within the vein media was found which correlated positively with p53 mRNA expression level. There was no increase of the apoptotic activity in elderly patients that led to the structural changes increase. In proximal GSV segments, despite SMC amount reduction or presence of structural changes in perivalvular wall region, no increase of the APIx with was noticed. P53-related apoptosis is one of the regulatory mechanisms of vein wall homeostasis maintenance. During varicose vein development its activation is related to the early stages of the disease. In the further course, the down-regulation of the SMC apoptosis within the vein media leads to the structural changes increase. The reduction of the SMC population corresponding to an increase of p21 expression in proximal saphenous vein segments suggests that the cell cycle disturbances may lead to the 'weakness' of the proximal GSV wall. Valve injury is not the only factor leading to the varicose veins occurrence.
    European Journal of Vascular and Endovascular Surgery 01/2005; 28(6):600-11. · 2.82 Impact Factor
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    ABSTRACT: A molecular mechanism responsible for varicose vein occurrence was investigated. The role of potential cell cycle regulator p21 and programmed cell death in the pathology leading to the proximal long saphenous vein (LSV) incompetence was investigated. Proximal LSV specimens were obtained from 40 patients with primary varicose veins who had undergone crossectomy. The expression of the p21, p53, and fas encoding genes was investigated by the means of real-time RT-QPCR. Immunostaining for gene product presence, proliferating cell nuclear antigen (PCNA), and apoptotic cells (TUNEL assay) was carried out. The results were compared to the control healthy vein specimens and correlated with pathologic examination findings (of the valve and vein structure). A significant increase in p21, p53, and fas mRNA expression were reported in the proximal incompetent veins. The expression of p21 correlated with expression of p53 (r = 0.658; p<0.05) and negative correlation between media apoptotic index and p21 mRNA expression was found (r = -0.493; p<0.05). Decrease in the muscular component within the media and disturbances of the local structure in the incompetent LSVs were reported. Fas overexpression did not correlate with p53 expression level and did not correlate with apoptotic cell number in the respective vein layers. PCNA-positive cells were present more frequently in the media of the control veins, especially in young subjects. Apoptosis downregulation, cell cycle inhibition and smooth muscle cell hypertrophy are important factors influencing vein wall disturbances related to sapheno-femoral junction incompetence.
    Clinical and Applied Thrombosis/Hemostasis 11/2004; 10(4):311-21. · 1.58 Impact Factor
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    ABSTRACT: The aim of this study was to determine whether Desulfovibrio desulfuricans-derived LPS stimulate the release of IL-6 and IL-8 from ECs and the expression of their adhesion molecules at the transcriptional level. Confluent monolayers of HUVEC were incubated in the absence or presence of 20 microg/ml and 60 microg/ml LPSs derived from the DdT and DdA bacterial strains. Also, the simultaneous stimulation of cells with LPSs and IL-1beta was evaluated. The levels of cytokines released were measured using ELISA. LPS-activated HUVEC increased the secretion of both IL-6 and IL-8, which was not LPS dose dependent. The expression of E-selectin and VCAM-1 was assessed by TR-PCR. The transcripts were detectable at all the concentrations (20, 40, 60 microg/ml) of LPSs used. These results suggest that D. desulfuricans LPS may activate immune functions in endothelial cells and influence the inflammatory response during bacteremia caused by these bacteria.
    Cellular & Molecular Biology Letters 02/2003; 8(4):991-1003. · 1.95 Impact Factor
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    ABSTRACT: Properties of butyltin complexes [Sn(C4H9-n)3{OOCC6H3(NH2)2-3,4}]n (1), [Sn(C4H9-n)3{OOCC6H3(NH2)2-3,5}] (2), [Sn(C4H9-n)3{OOCC6H4NNC6H4N(CH3)2-4}] (3) and [Sn(C6H5)3{OOCC6H3(NH2)2-3,5}]n (4) have been investigated. 1H, 13C and 119Sn NMR spectra indicate that the compounds in chloroform are distorted tetrahedral and in strongly coordinating solvents trigonal-bipyramidal complexes. Structure of complex 3 has been determined by X-ray crystallography. This compound adopts trigonal bipyramidal structure with bridging carboxylato ligand bound asymmetrically with tin atoms in axial positions. The complexes are effective cytostatic agents.
    Inorganica Chimica Acta 01/2003; 356:62-68. · 1.69 Impact Factor
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    ABSTRACT: Abbreviations used: IL-6 -interleukin-6; IL-8 -interleukin-8; DdA -Desulfovibrio desulfuricans wild intestinal strain DV-A/94; DdT -Desulfovibrio desulfuricans type soil strain; LPS -lipopolysaccharides; HUVEC -human umbilical vein endothelial cells; ECs -endothelial cells; VCAM-1 -vascular cell adhesion molecule-1; ELISA -enzyme-linked immunosorbent assay; RT-PCR -reverse transcription-polymerase chain reaction; PBS -phosphate buffered saline. Abstract: The aim of this study was to determine whether Desulfovibrio desulfuricans-derived LPS stimulate the release of IL-6 and IL-8 from ECs and the expression of their adhesion molecules at the transcriptional level. Confluent monolayers of HUVEC were incubated in the absence or presence of 20 μg/ml and 60 μg/ml LPSs derived from the DdT and DdA bacterial strains. Also, the simultaneous stimulation of cells with LPSs and IL-1β was evaluated. The levels of cytokines released were measured using ELISA. LPS-activated HUVEC increased the secretion of both IL-6 and IL-8, which was not LPS dose dependent. The expression of E-selectin and VCAM-1 was assessed by TR-PCR. The transcripts were detectable at all the concentrations (20, 40, 60 μg/ml) of LPSs used. These results suggest that D. desulfuricans LPS may activate immune functions in endothelial cells and influence the inflammatory response during bacteremia caused by these bacteria.
    CELLULAR & MOLECULAR BIOLOGY LETTERS Volume. 01/2003; 8:991-1003.
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    ABSTRACT: The properties of vinyltin and phenyltin complexes [Sn(CH=CH2)3{μ-OOCC6H3(NH2)2-3,4}]n (1), [Sn(C6H5)3{OOCC6H3(NH2)2-3,4}] (2), [Sn(C6H5)3{OOC-2-C6H4N=NC6H4N(CH3)2-4}] (3) and [Sn(CH=CH2)3{OOC-2-C6H4N=NC6H4N(CH3)2-4}] (4) have been investigated. The structures of complexes 1, 2, and 3, have been determined by X-ray crystallography. Compound 1 is a distorted trigonal-bipyramidal complex and compounds 2 and 3 adopt a distorted tetrahedral structure. Complex 1 is a single-strand polymer with a bridging 3,4-diaminobenzoato ligand coordinating via the O(1) atom of the carboxylato group and the nitrogen atom of the para-amino group. The oxygen and nitrogen atoms occupy the axial coordination sites. The Sn(1)−N(2A) bond is weak. In complexes 2 and 3 the carboxylato ligands are strongly coordinated to the central atom via one oxygen atom, and the Sn(1)−O(2) distances are considerably longer. Weak interactions of the central atom with the amino group in complex 1, and with the O(2) atoms in complexes 2 and 3, as well as the hydrogen bonds, stabilize the crystal structure. The complexes are effective cytostatic agents. (© Wiley-VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)
    Berichte der deutschen chemischen Gesellschaft 11/2002; 2002(12):3214 - 3221. · 2.94 Impact Factor
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    ABSTRACT: The properties and structures of the tetranuclear dibutyltin complexes [Sn4(µ3-O)2(C4H9-n)8­{OOCC6H3(NH2)2-3,4}4] (1), [Sn4(µ3-O)2(C4H9-n)8{OOCC6H3(NH2)2-3,5}4] (2), [Sn4(µ3-O)2(C4H9-n)8­{OOC-2-C6H4NNC6H4N(CH3)2-4}4] (3) are described. Complex 3 adopts a structure with a tetranuclear Sn4(µ3-O)2 core. All tin atoms are five-coordinate and form bonds with three oxygen atoms and two butyl ligands. Two carboxylates are bridging and two are terminal ligands. IR and NMR spectra indicate that the same structure is adopted by complexes 1 and 2. The molecular and electronic structures of complex 1 of Ci symmetry have been studied using the semi-empirical PM3 formalism. The calculated structure and bond distances agree with X-ray data. All complexes are effective antitumor agents. Copyright © 2002 John Wiley & Sons, Ltd.
    Applied Organometallic Chemistry 08/2002; 16(10):587 - 592. · 2.01 Impact Factor
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    ABSTRACT: The structure of the tin(III) complex [Sn(2)(CH(2)CH(2)CN)(6)] has been determined. There are two independent molecules in the crystal, both adopt distorted eclipsed conformation. The molecular and electronic structures of this compound have been studied both at the semiempirical level and with the use of non-empirical ab initio methods. The calculated Sn-Sn distances agree well with those found crystallographically. The results of calculations showed that the eclipsed conformation of complex is more stable as compared with staggered conformation. The compound show modest cytotoxic activity against A549 and HSMC cells.
    Journal of Inorganic Biochemistry 07/2002; 90(3-4):149-54. · 3.20 Impact Factor
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    ABSTRACT: The viability of the human arterial allograft cells depends on the time and method of vessel procurement and storage. In this study, an evaluation of the effect of the duration of 4 degrees C ischaemia and cryopreservation on human aortic and femoral artery allograft viability was performed. After the isolation of arterial wall cells, the identification of cultured cells was performed using mRNA analysis for estimation of smooth-muscle markers of differentiation: desmin and heavy-caldesmon. The viability of cells from the medial layer of the aortic wall ranged from 74 to 90% (61-79% for femoral arteries). Cold ischaemia time (from harvesting until the beginning of the preparation) is a statistically significant factor influencing smooth muscle cell viability. Smooth muscle cells represented the majority of live cell population.
    Folia Histochemica et Cytobiologica 02/2002; 40(2):217-8. · 1.10 Impact Factor