B Rak

Publications (2)9.2 Total impact

• Source

  Available from: nih.gov

  Article: Insertion element IS5 contains a third gene.

  B Rak, M von Reutern
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  ABSTRACT: We have previously shown that IS5 contains two genes encoded on opposite DNA strands within the same stretch of DNA. Here we present evidence that a third gene and its promoter are present on IS5. The newly discovered gene, ins5C , is contained within the longest gene of IS5, ins5A , but encoded by the complementary DNA strand. The three genes comprise a total of 519 codons present on the 1195-bp element. The arrangement of these genes represents a coding structure of unprecedented compactness.
  The EMBO Journal 05/1984; 3(4):807-11. · 9.20 Impact Factor
• Article: A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.

  B Rak
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  ABSTRACT: Presence of insertion element IS1 within an operon leads to absence of expression of genes distal to its integration site. This strong polar effect is observed irrespective of the orientation of IS1. Here I report on a mutant of E. coli K12 which allows turn-on of genes located distally to IS1. This turn-on is due to activation of transcription starting within IS1. The activation of transcription is under the control of a regulatory locus (sis1) which acts in trans. The regulatory locus itself changes its state, which leads to an alternating turn-off/turn-on of genes located distal to IS1: The rate of turn-off was observed with a frequency of about 0.3% (per cell and per generation), that of turn-on with a frequency of about 0.015% (per cell and per generation).
  MGG - Molecular and General Genetics 02/1983; 192(1-2):61-5.