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ABSTRACT: Cerebrospinal fluid (CSF) polyamines were measured in children with acute lymphocytic leukemia or non-Hodgkin's lymphoma in various stages of the disease ranging from complete remission to active central nervous system (CNS) involvement. Polyamines were analyzed by ion exchange chromatography with o- pthalaldehyde fluorimetric detection. Putrescine concentrations in random CSF samples obtained from leukemic patients with and without CNS involvement were not significantly different. Spermidine levels were generally higher in patients without CNS leukemia than in patients with the disease. In serial CSF specimens from an individual patient, spermidine levels correlated well with clinical status, being high in the active stages of the disease and low in remission. Thus, CSF polyamines appear to be of limited value as a screening test for early detection of CNS leukemia; however, they may offer an additional means of evaluating CNS leukemia and its response to therapy.
Annals of clinical and laboratory science 14(3):225-31. · 0.96 Impact Factor
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ABSTRACT: The automated diacetyl-l-napthol procedure was found to be a simple and relatively quick method for the determination of erythrocyte creatine (EC), exhibiting both better precision and greater sensitivity than reticulocyte counting. A reference range of 1.8 to 5.0 mg per dl (mean, 3.0 +/- 0.9 mg per dl) was established by measuring EC levels in 81 normal adults varying in ages from 20 to 47 years of age. Normal creatine values displayed a slight sex dependency, with females exhibiting somewhat higher levels than males. In evaluating the relative usefulness of EC versus reticulocyte counting in the assessment of anemia, both parameters were measured and compared in 41 patients with various hemolytic and nonhemolytic anemias. A direct relationship was noted between the two parameters (p less than 0.0001); however, the actual data correlation was only fair (r = 0.49). In a serial study of an iron-deficiency anemic patient responding to iron administration, erythrocyte creatine levels were found to rise more slowly than the reticulocyte count and to remain elevated after the reticulocyte count had returned to normal.
Annals of clinical and laboratory science 12(6):439-46. · 0.96 Impact Factor
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ABSTRACT: Erythrocyte creatine (EC) content and reticulocyte counts were compared in normal adults, pediatric patients with hemolytic anemia, and cord blood. A good correlation between reticulocyte count and EC content was found in normal subjects and patients with hemolysis, thus confirming the usefulness of creatine as an estimate of mean red cell age in these populations. No significant correlation (p greater than 0.1) was observed between the two measurements in cord blood. While reticulocyte counts were significantly elevated (p less than 0.001) in cord blood when compared to normal adults (indicating the presence of a young mean red cell age), EC concentrations in most samples were not correspondingly high. These results may indicate that creatine is not well synthesized by the neonatal red blood cell.
Annals of clinical and laboratory science 13(5):439-43. · 0.96 Impact Factor
