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ABSTRACT: High-throughput technologies such as microarray have enhanced the discovery of new biomarkers in prostate cancer. However, the reliability of transcriptome analyses is limited by the RNA quality.
Identification of variables influencing the RNA quality in radical prostatectomy specimens.
RNA was extracted using an automatic extraction method for 354 samples from 38 fresh frozen prostate slices, and by manual extraction for 28 samples from 5 slices. RNA quality was measured using the RIN method (RNA Integrity Number). Evaluation of tissue composition was performed by light-microscopy for each sample. Age, total operative time, estimated blood loss, prostate volume, prostate specific antigen (s-PSA) and postoperative Gleason score were registered. The independent variables were correlated to the RIN score in a multiple linear regression model, taking p < 0.05 as the significance limit.
The amount of blood loss during prostatectomy and the amount of stroma in the tissue sample both correlated negatively with the RIN score (p = 0.03 and 0.02). Automatically extracted samples which were exposed to heat according to the RNA extraction protocol, had lower mean RNA quality (5.5, 1.46 SD) than manually extracted samples, not exposed to heat (8.7, 0.86 SD), suggesting degradation by temperature sensitive RNases, mainly residing in the stroma.
The highest RNA quality isolated by an automatic method from fresh frozen prostate tissue is obtained from patients with low peroperative blood loss and from samples with a low stromal fraction.
Scandinavian journal of clinical and laboratory investigation 02/2010; 70(1):45-53. · 1.38 Impact Factor
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ABSTRACT: The medical case record is an important tool for securing high quality in treatment and care of patients. Efficient and accurate documentation of diagnostic and therapeutic procedures, patient information etc. is crucial.
We have prospectively studied how time and patient satisfaction are influenced when nurse and doctor together ("combined model") take the patient's medical history, perform a clinical examination, and inform the patient, compared to separate history-taking by nurse and doctor ("separate model").
The mean nurse time per patient increased from 17 min in the separate model to 21 min in the combined model (p = 0.01). Corresponding time use by the doctor was 26 min and 21 min (p = 0.04), and for the patient 43 min and 23 min (p < 0.001). Patients reported the two models to be comparable in quality.
The "combined registration model" secures high quality of the medical case record, respects the patient's time, and signals professional collaboration to the patient.
Tidsskrift for Den norske legeforening 09/2001; 121(21):2484-6.
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ABSTRACT: Acucise endopyelotomy has gained widespread use in the treatment of ureteropelvic junction obstruction. Acute postoperative bleeding is a well-known complication. We report one case with a delayed postoperative formation of pseudoaneurism, and one case which developed arterial hypertension postoperatively.
Scandinavian Journal of Urology and Nephrology 03/2000; 34(1):79-80. · 0.99 Impact Factor
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ABSTRACT: Androgens are implicated in the pathogenesis of prostatic carcinoma. We have elucidated the role of pre- and postnatal testosterone administration in the occurrence of proliferative lesions as well as neuroendocrine (NE) cells in the rat prostatic complex.
Female rats were given a single dose of 9 mg testosterone enantate i.m. on day 15 of pregnancy; it gave a high testosterone exposure to the fetus in the early organogenetic period of the rat prostatic complex. One group of the male offspring was followed without further testosterone treatment; a second group received testosterone only in the pubertal period; a third group was given testosterone from puberty and throughout life (46 weeks). These groups were compared to parallel groups (1A-1C) of male offspring without a testosterone supplement in pregnancy.
The serum testosterone concentrations in the rats receiving testosterone were significantly higher than those of control rats. Histopathologically, the testosterone-induced proliferative lesions, mainly hyperplastic, were almost exclusively located in the dorsal lobe. Chromogranin A-immunoreactive (CgA-IR) cells were rarely found normally, but occurred more often in the proliferative lesions (P < 0.001).
The incidence of proliferative lesions in rats exposed to testosterone only in puberty was comparable to the incidence found in those rats receiving testosterone in puberty and throughout life. This finding may have clinical implications for young athletes, who use testosterone as an anabolic drug. The occurrence of CgA-IR cells increased in proliferative lesions in the dorsal lobe of the rat prostatic complex.
The Prostate 08/1999; 40(2):65-75. · 3.48 Impact Factor
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The Journal of Pathology 06/1999; 188(1):113-4. · 6.32 Impact Factor
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ABSTRACT: Neuroendocrine (NE) cells are present in both benign and malignant human prostate. However, their function is poorly understood, mainly due to the lack of suitable experimental models. The nerve growth factor-beta (NGF-beta) promotes the rat pheochromocytoma cell line PC-12 to differentiate into neuronal like cells. We have studied the effect of NGF-beta on four human prostate cancer cell lines, LNCaP, DU-145, PC-3, and TSU-pr1. NGF-beta stimulates the growth rate in all these cell lines, but does not induce a neuronal phenotype. NE tumour markers (chromogranin A [CgA] and chromogranin B[CgB]) could not be demonstrated by immunocytochemistry (CgA and CgB), Northern blotting (CgA), or ELISA techniques (CgA), neither in control nor in NGF-beta stimulated cells. Consequently, other experimental models have to be sought in the study of NE cells in the human prostate.
Scandinavian Journal of Urology and Nephrology 03/1998; 32(1):7-13. · 0.99 Impact Factor
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ABSTRACT: The neuroendocrine (NE) cells in the human prostate gland probably have a local regulatory role in both prostatic growth and differentiation as well as in the exocrine secretory process. Moreover, NE cells may be involved in the pathogenesis of both prostatic cancer and hyperplasia. To enhance the knowledge of the physiological and pathophysiological role of NE cells in the prostate gland, we wanted to establish an experimental animal model.
All lobes of the prostatic complex of rats with different serum levels of testosterone, as well as the prostate of the guinea pig, cat, and dog, were studied. Prostatic tissue fixed in different fixatives was studied with regard to NE cells by using cytochemical and immunohistochemical staining techniques, as well as Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR) for detection of rat chromogranin A (CgA) mRNA.
The present study indicates the absence of NE cells in the rat prostatic complex. No expression of CgA RNA was detectable either by Northern blotting or by RT-PCR. Only a few argyrophil cells in the prostatic complex of guinea pig were detected in tissue fixed in Bouin's solution.
Rat, guinea pig, cat, and dog are not suitable animals in physiological studies of NE cells in the prostate gland.
The Prostate 10/1997; 33(1):18-25. · 3.48 Impact Factor
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ABSTRACT: Neuroendocrine (NE) differentiation of prostatic adenocarcinomas has received increasing attention in recent years as a result of possible implications on prognosis and therapy. The incidence of NE cells in tumors has been reported from 10% up to 100%. Several studies have shown chromogranin A (CgA) to be the most reliable serum marker of NE differentiation. We have followed 22 patients with prostatic adenocarcinoma over a 2-year period. The patients underwent a palliative transurethral resection of the prostate (TURP) because of urinary outflow obstruction. The prostatic tissue specimens were stained immunohistochemically using antibodies against CgA, chromogranin B (CgB), neuron-specific enolase (NSE), thyroid-stimulating hormone (TSH), serotonin, and somatostatin. In addition, each specimen was stained with hematoxylin & eosin (H & E), and saffran for tumor grading. Blood samples were taken preoperatively and after 1, 3, 6, and 24 months. The serum values of CgA, CgB, pancreastatin (Pst), NSE, and prostate-specific antigen (PSA) were determined from each sample. Carcinomas with groups of CgA-positive cells had higher serum levels of CgA compared to carcinomas with no or only scattered CgA-positive NE cells. During the 2-year period, there were no statistical significant variations in serum levels of CgA, NSE, Pst, and PSA. However, there was a significant increase in serum levels of CgB during the same period, P = 0.002, possibly due to an increase in number of NE cells in tumor or to a relative increase in production of CgB in the NE cells.
The Prostate 05/1997; 31(2):110-7. · 3.48 Impact Factor
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ABSTRACT: The aim of the present study was to examine the correlation between the immunohistochemical findings and the serum markers for neuroendocrine (NE) cells in patients with carcinoma of the prostate. Preoperative serum values of chromogranin A (CgA), chromogranin B (CgB), pancreastatin (Pst), neuron-specific enolase (NSE), and prostatic specific antigen (PSA) were determined in 22 patients. The tissue specimens were obtained by a palliative transurethral resection of the prostate (TURP) because of urinary outflow obstruction. Immunohistochemistry was performed by using antibodies against CgA, CgB, NSE,.serotonin, thyroid-stimulating hormone (TSH), and somatostatin. Tumor cells with NE differentiation were found in 91% of the cases. No patient had elevated serum values of NSE, despite the presence of NSE-positive tumor cells in 77% of the tumors. Neither did CgB in serum correlate with the immunohistochemical findings. Elevated serum values of CgA were found in 59% of patients. A positive correlation between the number of CgA-staining cells and the serum values of CgA was found, as seven out of eight patients with groups of CgA-positive tumor cells had elevated serum values of CgA. We conclude that CgA, in contrast to NSE, CgB, and Pst, seems to be a useful serum marker in predicting the extent of NE differentiation in prostatic tumors.
The Prostate 02/1997; 30(1):1-6. · 3.48 Impact Factor
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ABSTRACT: From March 1990 to November 1993, percutaneous endopyelotomy was performed in 22 patients for treatment of symptomatic ureteropelvic obstruction. One patient was treated bilaterally. In three patients renal pelvic stones were removed during the same procedure. Clinical findings, intravenous pyelography and isotope renography were considered in preoperative and postoperative evaluation. Overall, 18 of 23 procedures (79%) were successful. The success rate was lowest in patients with enlarged renal pelvis. Failed procedures became evident less than six months after operation.
Tidsskrift for Den norske legeforening 07/1995; 115(15):1828-9.
