Arpana Mittal

Kurukshetra University, Shahabad Deccan, Karnātaka, India

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Publications (8)3.43 Total impact

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    ABSTRACT: Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice straw was first treated with 0.5 M KOH to remove lignin followed by treatment with 0.1 N H2SO4 for removal of hemicellulose. The maximum carboxymethyl cellulase activity of 3.08 U/mL was obtained using 1% (w/v) pretreated rice straw with 1% (v/v) inoculum, pH 8.0 at 35°C after 60 h of growth under stationary conditions, while the same was obtained as 4.15 U/mL using 0.75% (w/v) pretreated substrate with 0.4% (v/v) inoculum, pH 8.0 at 30°C, under shaking conditions of growth for 48 h. For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively. The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.
    Biotechnology research international. 01/2014; 2014:651839.
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    ABSTRACT: Pyrazole and pyrazolone motifs are well known for their wide range of biological activities such as antimicrobial, anti-inflammatory, and antitumor activities. The incorporation of more than one pharmacophore in a single scaffold is a well known approach for the development of more potent drugs. In the present investigation, a series of differently substituted 4-arylidene pyrazole derivatives bearing pyrazole and pyrazolone pharmacophores in a single scaffold was synthesized. The synthesis of novel 4-arylidene pyrazole compounds is achieved through Knovenagel condensation between 1,3-diaryl-4-formylpyrazoles and 3-methyl-1-phenyl-1H-pyrazol-5-(4H)-ones in good yields. All compounds were evaluated for their in vitro antimicrobial activity. A series of 4-arylidene pyrazole derivatives was evaluated for their in vitro antimicrobial activity against two Gram-positive (Bacillus subtilis and Staphylococcus aureus) and two Gram-negative bacteria (Pseudomonas fluorescens and Escherichia coli), as well as two pathogenic fungal strains (Candida albicans and Saccharomyces cerevisiae). The majority of the compounds displayed excellent antimicrobial profile against the Gram-positive (B. subtilis and S. aureus), and some of them are even more potent than the reference drug ciprofloxacin.
    Organic and medicinal chemistry letters. 08/2013; 3(1):9.
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    ABSTRACT: The production of poly β -hydroxybutyrate (PHB) by Bacillus subtilis NG220 was observed utilizing the sugar industry waste water supplemented with various carbon and nitrogen sources. At a growth rate of 0.14 g h(-1) L(-1), using sugar industry waste water was supplemented with maltose (1% w/v) and ammonium sulphate (1% w/v); the isolate produced 5.297 g/L of poly β -hydroxybutyrate accumulating 51.8% (w/w) of biomass. The chemical nature of the polymer was confirmed with nuclear magnetic resonance, Fourier transform infrared, and GC-MS spectroscopy whereas thermal properties were monitored with differential scanning calorimetry. In biodegradability study, when PHB film of the polymer (made by traditional solvent casting technique) was subjected to degradation in various natural habitats like soil, compost, and industrial sludge, it was completely degraded after 30 days in the compost having 25% (w/w) moisture. So, the present study gives insight into dual benefits of conversion of a waste material into value added product, PHB, and waste management.
    BioMed research international. 01/2013; 2013:952641.
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    ABSTRACT: The present study describes the treatment of sugar industry waste water and its use as a potential low cost substrate for production of bioplastic by Bacillus subtilis NG05. The B. subtilis NG05 grow at the rate of 0.14 g h−1 L−1 of production media used and accumulate the 50.1 % of poly β-hydroxybutyrate (PHB). The phase contrast microscopy revealed the presence of PHB granules in B. subtilis NG05 which was further confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance. The polymer was further analysed by differential scanning calorimetry. PHB production yield was achieved up to 4.991 g L−1 with Sugar industry waste water as sole nutrient source. Thus the process provided dual benefits of conversion of a waste material into value added product, PHB and waste management.
    Journal of Polymers and the Environment 01/2012; · 1.50 Impact Factor
  • jundishapur journal of Microbiology. 01/2012;
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    ABSTRACT: The amount of plastic waste increases every year and exact time for its degradation is unknown. Out of a total of 300 isolated strains one strain was selected for PHB production in different conditions like carbon source, nitrogen source and incubation temperature and time. When PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with raffinose and peptone. Regarding incubation time and temperature and pH, optimum PHB production conditions were 72h, 30°C and 7.0, respectively. The isolate was characterised biochemically as Bacillus species. The present study provide the useful data about the optimized conditions for PHB production by Bacillus species that can be utilized for industrial production of PHB, a fast emerging alternative of non biodegradable plastics.
    European Journal of Biological Sciences. 12/2011; 3(4):112-116.
  • Innovative Romanian Food Biotechnology. 01/2011;
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    ABSTRACT: 1-(2-Thienyl)-2-arylazo-4,4,4-trifluorobutane-1,3-diones 4, obtained by the condensation of aryldiazonium salts 2 with 1-(2-thienyl)-4,4,4-trifluoromethyl-1,3-butanedione 3, on treatment with hydroxylamine hydrochloride yielded 3-(2-thienyl)-4-arylazo-5-hydroxy-5-trifluoromethyl-Δ2-isoxazolines 5. Subsequently, dehydration of 5-hydroxy-5-trifluoromethyl-Δ2-isoxazolines 5 to corresponding 3-(2-thienyl)-4-arylazo-5-trifluoromethylisoxazoles 6 was achieved by treating them with acetic anhydride or EtOH–H2SO4. The structure of the compounds was established on the basis of IR, NMR (1H, 13C and 19F), mass spectral studies and elemental analyses. All the compounds were screened for their in vitro antibacterial activity against four pathogenic bacterial strains such as Bacillus subtilis, Staphylococcus aureus (Gram-positive), Pseudomonas fluorescens, Escherichia coli (Gram-negative) and in vitro antifungal activity against two pathogenic fungal strains namely, Candida strain, Saccharomyces cerevisiae. Most of the compounds showed moderate to significant antibacterial activity selectively against Gram-positive bacteria and antifungal activity against S. cerevisiae.
    Journal of Fluorine Chemistry 145:95–101. · 1.94 Impact Factor