[Show abstract][Hide abstract] ABSTRACT: This study examined the potential effects of Saponaria officinalis (SO) or Panax ginseng (PG)
saponins supplemented to diets differing in the proportion of forage to concentrate on rumen
microbial fermentation and methane production in vitro. Two experiments were carried out using
the rumen simulation technique (RUSITEC). In the first experiment the substrate was comprised
of a mixture of meadow hay and corn meal in a ratio of 60:40 dry matter (DM; high forage diet;
HF). In the second experiment low forage diet (LF) consisting of meadow hay and corn meal in
the ratio of 40:60 DM was used. Diets were supplemented with dried roots of SO or PG to provide
1% of triterpenoid saponins in dietary dry matter. All triterpenoid sources significantly (P≤0.05)
decreased number of protozoa, by 50% and 72% respectively when HF diet was analysed (first
experiment). There were no changes in methane production. In the second experiment (LF), the
potential to mitigate methane production was reported for both SO and PG addition, however
only in PG a decrease in the protozoal population was detected. Supplementation of plants rich in
triterpenoid saponins has been identified as a diet dependent potential factor which has an important
role in modulation of rumen fermentation processes. However, further studies are needed to
evaluate their effect in animal production.
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to evaluate the effect of Mentha piperita L. (MP) dried leaves on the in vitro rumen methanogenesis and fermentation. It was found that after 24 hours of incubations, addition of 16.34 and 23.35 mg of MP to the 233.3 mg of substrate significantly decreased methane emission by 41.52 and 15.51%, respectively. Simultaneously, the MP supplementation exerted no effect on the dry matter digestibility and volatile fatty acid profile. Addition of MP to the ruminants' diet inhibits the methane production without altering the basic parameters of rumen fermentation.
[Show abstract][Hide abstract] ABSTRACT: There is a great interest in reducing the methane emission from ruminants as one possible cause of global warming. The aim of the presented study was to determine the effects of xanthohumol, one of the hop (Humulus lupulus) phytofactors, on methane production, microbial population and basic parameters of ruminal fermentation. The experiment was carried out in a batch culture system. The basic substrate (400 mg) consisting of meadow hay and barley meal (60:40) was supplemented with 0 (Control), 0.1, 0.2 or 1.0 mg of xanthohumol. The basic parameters of rumen fermentation and composition of microbial population were measured after 24 h of incubation. For the first time, the results of this in vitro study have demonstrated that xanthohumol is capable to reduce the methane production, even at the lowest dose applied (0.1 mg/400 mg). The observed reduction in methane production by 12-13% was not accompanied by altering the basic rumen fermentation parameters. However, the practical utility of this supplement needs further investigation under long-term in vivo conditions.