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ABSTRACT: Based on the concept of magnetofection, we prepared lipid shell
microbubbles loaded with highly positively charged iron oxide magnetic
nanoparticles through electrostatic and matrix affinity interactions.
These magnetic microbubbles showed strong ultrasound contrast. When the
magnetic microbubbles were mixed with plasmid DNA encoding a reporter
gene, gene delivery to HeLa cells was achieved only when ultrasound was
applied. Gene transfer efficiency strongly depended on the application
of a gradient magnetic field. Treatment of HeLa cells with the
microbubbles and ultrasound resulted in strong concentration-dependent
cytotoxic effects, whereas ultrasound alone, lipid microbubbles alone,
magnetic nanoparticles or magnetic microbubbles alone did not
significantly affect cell viability. These magnetic microbubbles could
be used as magnetically targeted diagnostic agents for real-time
ultrasound imaging or for cancer therapy, therapy of vascular thrombosis
and gene therapy.
12/2010; , ISBN: 0094-243X
AIP Conference Proceedings. 01/2010; 1311(1):485-494.
Human Gene Therapy. 01/2010; 21(9):1191-1191.
Human Gene Therapy. 21(10):1429-1430.