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Eye (London, England) 09/2008; 23(7):1606-7. · 1.97 Impact Factor
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ABSTRACT: A gene (AglyA) encoding serine hydroxymethyltransferase of Acinetobacter radioresistens CMC-1 was cloned and sequenced. Nucleotide sequence analysis of AglyA predicted a single open reading frame (ORF) of 1251 bp encoding a 417-amino acid polypeptide. Two putative MetR-like binding sites (5'-TGAAACATGAGCT) and (5'-TGAGCAAAGTTCA), centered at bp -123 and -95 relative to the +1 translation start site were found, which have six out of nine and eight out of nine nucleotides that match to the consensus sequence of Escherichia coli (5'-TGAANNT/ANNTTCA), respectively. The enzyme also showed a high level of homology to other sources of serine hydroxymethyltransferase proteins.
FEMS Microbiology Letters 02/1999; 170(2):413-8. · 2.04 Impact Factor
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ABSTRACT: The gene for the creatinase from Pseudomonas putida NTU-8 was sequenced and revealed an open reading frame (ORF) of 1209 base pairs encoding a polypeptide of 403 amino acids with a calculated molecular weight (M(r)) of 45,691. The deduced amino acid sequence is very similar to that of the creatinase of Pseudomonas putida and Flavobacterium sp. An overproduction system for the chitinase signal peptide--creatinase hybrid gene was constructed by using the pQE-51 expression vector in E. coli JM109. The amount of this fusion enzyme was about 50% exported into the periplasmic space of E. coli.
Biochemical Genetics 01/1999; 36(11-12):407-15. · 0.86 Impact Factor
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ABSTRACT: Neocarzinostatin is an enediyne antitumor antibiotic. Upon attack by a thiol, the enediyne nucleus is cycloaromatized into two stable 1:1 thiol adducts. After analyzing products from various thiols, the chromatographic and spectroscopic characters that associate only with the cyclized aromatic moiety from enediyne nucleus were assigned. Based on HPLC analysis we have derived, products from picomole ranges of the drug sample can be detected. Confirming the type of cycloaromatization at nanomole ranges can be achieved by photodiode array UV spectroscopy. Three-dimensional fluorogram presents ten times more sensitive identification. The method provides a sensitive tool for massive screening study in microscale.
Biochimica et Biophysica Acta 08/1997; 1336(1):43-50. · 4.66 Impact Factor
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ABSTRACT: This study investigated school children in the Tung-Lo Township in central Taiwan to determine the prevalence of goiters compared to other areas of Taiwan and to elucidate the possible etiology. All children attending elementary schools in Tung-Lo were examined for thyroid enlargement by neck palpation, as recommended by the World Health Organization. Thyroid antibodies and thyroid hormones were determined in children with goiters, and in normal age- and sex-matched controls. Additionally, analysis of drinking water for humic substances was done by fluorospectrophotometry. In total, 1,823 school children were examined (965 boys and 858 girls). Of these, 174 (9.5%) were found to have a goiter of grade I or above. The prevalence was higher than our recent surveys in other areas of Taiwan (2.6%-8.8%). Goiter prevalence in school children residing in hill regions (12.9%), mainly dependent on underground water for drinking, was higher than that of school children residing in plain regions (8.2%), who depend on tap water for drinking. The quality of drinking water bore a close relationship to the prevalence of goiter. From the ratio of T3/T4 in this study, and a study of urinary iodine excretion done by others, it is concluded that goiters in Tung-Lo are not related to iodine deficiency. There was no statistically significant correlation between the relative fluorescence intensity of humic substances and the prevalence of goiter. The results of this study suggest that the higher prevalence of goiter in children living in Tung-Lo, an oil-bearing area, may be related to the quality of drinking water.
Journal of the Formosan Medical Association 11/1995; 94(10):622-5. · 1.13 Impact Factor
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ABSTRACT: Salt has been iodized in Taiwan since 1967. To understand goiter status after salt iodization, we studied the prevalence of goiter in school children in rural areas where tap water or underground water are used. To further elucidate the prevalence of goiter in urban areas where tap water is used, we studied school children from the Tong-Yuan and Lao-Song elementary schools located in the Wan-Hwa District of Taipei. Thyroid enlargement was examined by palpation as recommended by the World Health Organization. Thyroid antibodies and hormones were determined in school children with goiter and in age- and sex-matched normal control children using particle agglutination methods and radioimmunoassay. A total of 4,623 school children were examined. Of these, 129 children (2.8%) had goiters of grade I or above, or nodular goiters. The prevalence of goiter was similar to that of Putai and Peimen in southern Taiwan where tap water is used, but less than that of Tachia and Chingshuei in middle Taiwan where underground water is used. The prevalence of goiter was much lower than recent studies in Tung-Lo, an oil-bearing area in middle Taiwan, where there was a 12.9% prevalence in school children residing in hill districts who relied on underground water for drinking purposes, and 8.2% in school children residing on lower plains, who depended upon tap water for drinking purposes. The prevalence of goiter is highly related to the quality of drinking water.(ABSTRACT TRUNCATED AT 250 WORDS)
Journal of the Formosan Medical Association 05/1995; 94(4):193-6. · 1.13 Impact Factor
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ABSTRACT: Blackfoot disease is an endemic peripheral vascular disorder which is confined to a limited land area on the southwest coast of Taiwan. It has long been related to the consumption of high levels of arsenic found in the artesian well water. Humic substances have also been extracted from the well water and have been reported as a possible source of environmental goitrogen. The purpose of this study was to examine whether the prevalence of goiter is increased in the blackfoot disease-endemic area. This study covered all the children in the elementary schools of Putai and Peimen. They were divided into two groups according to the location of schools in the endemic area or non-endemic area of blackfoot disease. Thyroid enlargement was examined by palpation as recommended by the World Health Organization. Thyroid antibodies and hormones were determined in school children with a goiter and age-sex-matched normal control children using particle agglutination methods and radioimmunoassays, respectively. Aspiration cytology was done in cases with a nodular goiter. In total 4,567 school children were examined, including 2,306 males and 2,261 females. One hundred and twenty school children (2.63%) had a goiter of grade I or above. The prevalence of goiters in school children from the endemic area was higher than that from the non-endemic area (3.44 vs 2.08%, p less than 0.01). The prevalence of goiters in females from the endemic area was higher than that from the non-endemic area (4.65 vs 2.69%, p less than 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)
Journal of the Formosan Medical Association 11/1991; 90(10):941-6. · 1.13 Impact Factor
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ABSTRACT: The en route monitor system (ERMS), which is a software system
designed to assist human controllers, was developed as a tool for the
control center. A domain-specific knowledge-based design approach was
used. The system is implemented in Turbo Prolog. The problem and
solution techniques are discussed in detail, and examples and
simulations are demonstrated to show the potential application of the
ERMS in air traffic control
IEEE Transactions on Aerospace and Electronic Systems 06/1989; · 1.10 Impact Factor
