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ABSTRACT: Radiosensitivity is a biological response to radiation. This response depends on many factors such as radiation factors as well as biological system factors. It is shown that identical doses of radiation for the treatment of Cancer patients produce different biological responses that are assumed to be depend on different specifications of the biological systems. However, by elimination of these fac-tors, people may still show different biological responses such as acute and low responses to radiotherapy in similar doses of radiation. Some reports indicate that breast cancer, immune diseases including autoimmune diseases such as lupus, Myasthenia Gravies and even the rate of allergy are more frequent in left-handed compared to right-handed individuals. The main goal of the present study is deter-mination of radiosensitivity in left-handed compared to right-handed in breast cancer women by cytogenetic assay. Peripheral venous blood samples (10 ml) of 30 breast cancer women (10 left-and 20 right-handed) were divided into two identical parts. One part is exposed to 2 Gy Co-60 gamma rays, and the second part is considered as non-exposed controls. Lymphocytes were cultured in standard media, and cytokinesis blocked to score micronuclei in bi-nucleated cells. The frequency of micronuclei in 1,000 cells in each sample is considered as the rate of radiosen-sitivity and was compared in left-and right-handed breast cancer women by appropriate statistical analysis. Results showed that radiosensitivity index in left-handers is higher than right-handers also mean frequency of MN in exposed group of left-handers compare to right-handers is elevated. It seems that left-handed breast cancer women are more radiosensitive than right-handed. More investigations on right-and left-handed healthy people are ongoing in our laboratory.
Medical Oncology 03/2013; · 2.14 Impact Factor
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ABSTRACT: Arbutin has antioxidative properties with potential benefits in many diseases. In this study, the cytoprotective and antioxidant effects of arbutin against t-BPH induced oxidative stress in Hep G2 cells were evaluated. HepG2 cells were cultured in 24-well plates (2.5×10 cells in 1 ml RPMI-1640 + 10% FBS+1% Pen. 5 Strep). Arbutin at different concentrations (0, 50, 100 and 150 µM) were added into the wells. After 24 h exposure to arbutin, t-BHP at different concentrations (0, 100, 150, 200 and 250 µM) were added. Viability was measured (t = 72 h) using MTT assay and TB test. Furthermore, the antioxidant effect of arbutin was evaluated by FRAP assay. Arbutin significantly increased the total antioxidative power and antagonized the t-BHP oxidative effects. The cytotoxicity of t-BHP was significantly reduced by arbutin pretreatment. In the TB test and MTT assay, there was a significant difference between cell viability of group containing arbutin and t-BHP with group containing only t-BHP in a dose-dependent manner. Arbutin increased the total antioxidative power and it had cytoprotective effect. Increasing of cell viability might be an explanation to these effects.
world applied sciense. 03/2013; 19:163-167.
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07/2012;
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05/2012;
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01/2012;
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[show abstract]
[hide abstract]
ABSTRACT: Radiosensitivity is a biological response to radiation. This response depends on many factors such as radiation factors as well as biological system factors. It is shown that identical doses of radiation for the treatment of Cancer patients produce different biological responses that are assumed to be depend on different specifications of the biological systems. However, by elimination of these factors, people may still show different biological responses such as acute and low responses to radiotherapy in similar doses of radiation. Some reports indicate that breast cancer, immune diseases including autoimmune diseases such as lupus, Myasthenia Gravies and even the rate of allergy are more frequent in left-handed compared to right-handed individuals. The main goal of the present study is determination of radiosensitivity in left-handed compared to right-handed in breast cancer women by cytogenetic assay. Peripheral venous blood samples (10 ml) of 30 breast cancer women (10 left- and 20 right-handed) were divided into two identical parts. One part is exposed to 2 Gy Co-60 gamma rays, and the second part is considered as non-exposed controls. Lymphocytes were cultured in standard media, and cytokinesis blocked to score micronuclei in bi-nucleated cells. The frequency of micronuclei in 1,000 cells in each sample is considered as the rate of radiosensitivity and was compared in left- and right-handed breast cancer women by appropriate statistical analysis. Results showed that radiosensitivity index in left-handers is higher than right-handers also mean frequency of MN in exposed group of left-handers compare to right-handers is elevated. It seems that left-handed breast cancer women are more radiosensitive than right-handed. More investigations on right- and left-handed healthy people are ongoing in our laboratory.
Medical Oncology 01/2012; 29(4):2552-5. · 2.14 Impact Factor
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05/2011;
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ABSTRACT: The High Natural Background Radiation Area (HNBRA) of Ramsar has been the subject of concern in the last 40 years for a high level of radiation measured in some spots as high as 260 mSv per year. Studies of health status of Ramsar inhabitants did not show significant increase in the frequency of cancer. In this study, some aspects of the immune surveillance in the HNBRA residents of Ramsar were investigated. Our results showed that the CD4+ and CD8+ percentage in residents of HNBRA was higher compared to the Ordinary Natural Background Radiation Area (ONBRA) inhabitants. However, the difference between CD4+ also CD8+ cells count and CD4/CD8 ratio in two areas was not significant statistically. These findings may indicate that the immune systems of these people are adapted but more studies to compare the function of immune systems between two groups become essential.
International Journal of Low Radiation. 01/2011; 8(4):329 - 337.
