Mónica Germano

Publications (2)4.98 Total impact

• Source

  Available from: Sheila Ons

  Article: Identification of a point mutation associated with pyrethroid resistance in the para-type sodium channel of Triatoma infestans, a vector of Chagas' disease.

  Jésica Fabro, Marcos Sterkel, Natalia Capriotti, Gastón Mougabure-Cueto, Mónica Germano, Rolando Rivera-Pomar, Sheila Ons
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  ABSTRACT: The voltage-gated sodium channel is the target site of pyrethroid insecticides. Point mutations in the domain II region of the channel have been implicated in pyrethroid resistance of several insect species. We identified the sequence of domain II from the para sodium channel in Rhodnius prolixus, a vector of Chagas' disease. With this information, we cloned and sequenced the domain II of the sodium channel from the other main Chagas' disease vector: Triatoma infestans. We also identified the presence of a resistance-conferring mutation (L1014F) in a pyrethroid-resistant population of T. infestans from Argentina, and present a PCR-based method to detect this mutation in individuals from field populations. These findings have important implications for the implementation of strategies for resistance management, and for the rational design of campaigns for the control of Chagas' disease transmission.
  Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 12/2011; 12(2):487-91. · 3.22 Impact Factor
• Article: Susceptibility of sylvatic Triatoma infestans from Andeans valleys of Bolivia to deltamethrin and fipronil.

  Gonzalo Roca Acevedo, Gaston Mougabure Cueto, Mónica Germano, Pablo Santo Orihuela, Mirko Rojas Cortez, François Noireau, María Inés Picollo, Claudia Vassena
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  ABSTRACT: We describe the susceptibility to deltamethrin and fipronil of four sylvatic Triatoma infestans populations from the Andean valleys of Bolivia. Fifty percent lethal doses were determined from topical application of insecticide on first instars, and mortality was assessed after 24 h for deltamethrin and 48 h for fipronil. In comparison with a reference strain from Argentina, the Bolivian populations showed deltamethrin 50 percent lethal dose ratios ranging from 1.9 to 17.4. In the case of fipronil, an insecticide never used for control of T. infestans, the Bolivian populations showed even higher variation in toxic response, with relative susceptibilities ranging from 0.5 to 139.2. However, although the sylvatic T infestans toxicological profiles differ from each other and from those of the domiciliary population studied in this work, there were no significant differences in the activities of P450 mono-oxygenases and pyrethroid esterases between the reference strain and the studied populations.
  Journal of Medical Entomology 07/2011; 48(4):828-35. · 1.76 Impact Factor