Junda Lin

Publications (30)36.48 Total impact

• Article: Fecundity, brood loss and egg development through embryogenesis of Armases cinereum (Decapoda: Grapsidae)

  Joana Figueiredo, Gil Penha-Lopes, Justin Anto, Luís Narciso, Junda Lin
  [show abstract] [hide abstract]
  ABSTRACT: The present work is a comprehensive study of reproduction and embryonic development of Armases cinereum. Ovigerous A. cinereum (Bosc, 1802) females from Sebastian Inlet, Florida (9.88–19.4mm CW) lay 2,000–12,000 eggs per brood, depending on their CW (mm): fecundity=24.662CW1.9432. A. cinereum displayed significant brood loss through development (ca. 500 eggs per brood) independently from their CW (no senescence). However, since smaller females lay fewer eggs than larger ones, the percentage of eggs lost during embryonic development is greater in smaller females. The number of eggs carried on a later stage of development (potential fertility=5.5593CW2.4417) is a more accurate estimate of the reproductive output and subsequent recruitment. Egg volume increased during development (64%, 0.025–0.041mm3 or 0.36–0.43mm of diameter, N=270) and was strongly correlated with egg water content increase (19.21%, r=0.89). Lipids, particularly fatty acids, seem to be the major energy source for embryonic development, decreasing 56.31 and 37.08% (respectively) during embryonic development; both are negatively correlated with egg volume (r=−0.90). The utilization of fatty acids through the different developmental stages of A. cinereum is presented. The most consumed fatty acids are the monounsatured (43.33μgmg−1dw), followed by the saturated (29.91μgmg−1dw) and polyunsaturated (24.03μgmg−1). Palmitic (16:0) and linoleic (18:2n-6) acids are preferentially consumed (19.5 and 17.9μgmg−1dw, respectively). The high proportion of essential polyunsaturated fatty acids of C18 and C20 reflects the consumption of primary producers such as mangrove leaves. EPA/DHA ratio (2.85–3.84) and low DHA content indicated that this species appears in a medium-low level of the trophic chain. The low ratio of 18:1n-7/18:1n-9 and high percentage of 18:1n-9 (marker of carnivory) may be a sign of the consumption of juvenile invertebrates. The high percentage of odd-numbered FA indicated the occurrence of detritivores/scavenger behaviours. The fatty acid composition of the eggs reflects adult feeding ecology (omnivorous) and habitat.
  Marine Biology 04/2012; 154(2):287-294. · 2.28 Impact Factor
• Article: Comparative mating success of smaller male-phase and larger male-role euhermaphrodite-phase shrimp, Lysmata wurdemanni (Caridea: Hippolytidae)

  Dong Zhang, Junda Lin
  [show abstract] [hide abstract]
  ABSTRACT: The protandric simultaneous hermaphrodite shrimp Lysmata wurdemanni (Gibbes 1850) has a pure searching mating system, i.e., males are continually searching for receptive females and copulation is brief. To examine whether size-based advantage in male–male competition occurs and whether the mating ability of male-phase (M) shrimp equals that of euhermaphrodite-phase shrimp serving as males (Em), mating performance, including mating frequency and precopulatory behavior, of M and Em shrimp was compared using two M:Em ratios. Two experiments were carried out from March 2004 to August 2004at Florida Institute of Technology’s Vero Beach Marine Laboratory using laboratory-cultured shrimp that originated from Port Aransas, TX, USA. In the two experiments, one parturial euhermaphrodite-phase shrimp acting as a female (Ef) was maintained with one M and two Em shrimp (one with and one without an egg mass), and two M and two Em shrimp, respectively. The M shrimp used were always smaller than the Em shrimp. Experiment 1 showed that there was no significant difference in mating ability between Em with and without egg mass. In both experiments, the M shrimp gained mating partners more frequently than the Em shrimp did. In the experiment with two M and two Em shrimp, mating frequencies of the small M and large M shrimp were similar. Precopulatory behaviors of the M shrimp were more active than those of the Em shrimp. Mating between the small M and larger Ef shrimp was sometimes successful even when the size difference was 20.0mm total length (TL). Mating between a larger M shrimp and smaller Ef shrimp sometimes failed when the size difference was only 13.0mm TL. Mating frequency of M shrimp over that of Em shrimp with Ef shrimp increased significantly with increasing density and operational sex ratio. The advantage of M over Em shrimp in obtaining mating partners is probably a result of sexual selection and adaptation, and may partially explain the observed delayed sex change in some L. wurdemanni, i.e., some male-phase shrimp grow very large and never become hermaphrodites.
  Marine Biology 04/2012; 147(6):1387-1392. · 2.28 Impact Factor
• Article: Effect of ferric ion on nitrogen consumption, biomass and oil accumulation of a Scenedesmus rubescens-like microalga.

  Qiang Lin, Na Gu, Junda Lin
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  ABSTRACT: This investigation examined the effects of ferric ion source and concentration on the biomass yield, fatty acid methyl esters (FAME) productivity and composition from Scenedesmus rubescens-like microalga. The algae cultivated with Fe(2+)-SO(4)(2-)/EDTA had higher ash free dry biomass (AFDB) production and in vivo chlorophyll a concentration than those with Fe(2+)-SO(4)(2-) and Fe(3+)-Cl(-) (P<0.001). The high or low Fe(2+) concentration can partly restrict the ammonia consumption, the AFDB and FAME accumulation. The algae at 1mg/L Fe(2+) concentration had the highest AFDB and FAME productivity (341.1 ± 25.8 and 107.8 ± 19.3mg/L/d) (P<0.001). The mean content of C16 and C18 series at all Fe(2+) concentrations was 88.6% ± 2.9% of total FAME, and it decreased with the increase of Fe(2+) concentrations. Among the fatty acids profiles, the contents of C18: 0, C18: 1n-7 and C18: 1n-9 are significantly changed under the different Fe(2+) concentrations, but not the contents of C16 series.
  Bioresource technology 02/2012; 112:242-7. · 4.25 Impact Factor
• Article: Higher efficiency of CO2 injection into seawater by a venturi than a conventional diffuser system.

  Hong Du, Junda Lin, Chris Zuercher
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  ABSTRACT: Mass production of microalgae generally requires the injection of CO(2) into open ponds or photo-bioreactors. The present study compares the CO(2) injection efficiency into seawater of a porous stone air diffuser and a venturi. CO(2) was injected at flow rates of 400, 700 and 1000 standard mL/min and 4, 7 and 10 standard L/min into a small and a large pond, respectively until the pH decreased from 7.8 to 6.8. No significant differences in CO(2) injection efficiency between the three CO(2) flow rates (p>0.05) were observed; however, CO(2) injection efficiency with venturi was about 100% (p<0.05) higher than that of the air diffuser. Therefore, it is possible to both reduce the cost and increase the effectiveness of CO(2) dissolution in seawater by using venturi operated at a lower flow rate, i.e. 400 standard mL/min in a small pond and 4 standard L/min in a large pond.
  Bioresource technology 12/2011; 107:131-4. · 4.25 Impact Factor
• Article: Biomass production and fatty acid profile of a Scenedesmus rubescens-like microalga.

  Yixin Tan, Junda Lin
  [show abstract] [hide abstract]
  ABSTRACT: This investigation examined the effects of nitrogen-phosphate combined deficiency on the biomass yield, fatty acid methyl esters (FAME) production and composition from Scenedesmus rubescens-like microalga. A 15-day indoor culture was performed as a 3 × 3 factorial design (NaNO(3) levels: 3, 10 and 20mM; KH(2)PO(4) levels: 20, 50 and 150 μM). The algae grown under medium nitrogen concentration (10mM) and high phosphate concentration (150 μM) reached the highest biomass (1223.5 ± 152.5mg/L). Both nitrogen and phosphate had a significant influence on the FAME yield (P<0.05 and P<0.0001, respectively). The FAME yield from algae grown under low nitrogen (3mM) and phosphate concentration (20 μM) increased throughout the experiment and the highest FAME yield (42.2 ± 2.5% of AFDW) as well as C16 and C18 content (95.8 ± 1.6% of AFDW) was achieved under these conditions. Algae grown under medium nitrogen concentration (10mM) and low phosphate concentration (20 μM) had the highest FAME productivity (426.0mg/L ± 135.0mg/L). Thus, the lower nitrogen concentration (3mM-10mM) and low phosphate concentration (20 μM) would be an optimal combination tested to produce the most FAME from S. rubescens-like algae.
  Bioresource technology 07/2011; 102(21):10131-5. · 4.25 Impact Factor
• Article: Effects of nitrogen source and concentration on biomass and oil production of a Scenedesmus rubescens like microalga.

  Qiang Lin, Junda Lin
  [show abstract] [hide abstract]
  ABSTRACT: This investigation examined the effects of nitrogen source and concentration on the ash free dry biomass (AFDB) and oil accumulation and productivity of a Scenedesmusrubescens like alga cultivated in indoor photo-bioreactors and outdoor raceway ponds. Among the nitrogen sources ((NH4)2CO3, urea, NaNO3, urea and NaNO3 mixture), the algae fed with the mixture of urea-N and NaNO3-N had the highest AFDB productivity of 0.539±0.040 g/L/d and the content of fatty acid methyl esters (FAME) (%) fed with (NH4)2CO3-N increased continuously for 17 days and reached 42.94±2.05% in the indoor photo-bioreactors. The content of C18 series (as % of total FAME) was high in all the treatments of nitrogen sources. The algae fed with (NH4)2CO3-N had the highest FAME productivity of 0.133 g/L/d (P<0.001). The effect of ammonia concentration on the AFDB and FAME productivity was also studied during the 20-day cultivation in the outdoor raceway cultivation system. The mean FAME of the algae cultivated in the ammonia concentration of 1.0-1.5 mg/L were the highest (0.556±0.112 g/m2/d) (P<0.001). The total Kjeldahl nitrogen (TKN) in the algae and cultivation water decreased but the FAME content (%) of the algae increased during the nitrogen starvation phase.
  Bioresource technology 09/2010; 102(2):1615-21. · 4.25 Impact Factor
• Article: Growth and survival of juvenile lined seahorse, Hippocampus erectus (Perry), at different stocking densities

  Dong Zhang, Yinghui Zhang, Junda Lin, Qiang Lin
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  ABSTRACT: The lined seahorse, Hippocampus erectus (Perry), is an important species in both medicinal and aquarium trades. The aim of this study was to evaluate the effects of stocking density (1, 3 and 5 individuals L−1) on the growth performance and survival of the early-stage juvenile H. erectus. The height (HT), wet weight, weight gain (WG) and specific growth rate (SGR) were affected significantly by the stocking density during the 40-day study. The HT, WG and SGR of the seahorse at 1 and 3 juveniles L−1 were significantly higher than that at 5 juveniles L−1. The survival of juveniles at the three stocking densities was not significantly different at day 25 (90.3 ± 4.5%, 86.7 ± 4.2% and 86.2 ± 3.8% for 1, 3 and 5 juveniles L−1 respectively), but was significantly different at day 40 (87.8 ± 3.9%, 69.6 ± 4.2% and 52.9 ± 2.8% for 1, 3 and 5 juveniles L−1 respectively). For the early-stage juvenile H. erectus, we recommend a stocking density of 3 juveniles L−1, but the density should be reduced to 1–2 juveniles L−1 to avoid reduced and variable growth and high mortality after 25 days.
  Aquaculture Research 03/2010; 42(1):9 - 13. · 1.20 Impact Factor
• Article: Reproductive isolation between two sympatric simultaneous hermaphroditic shrimp, Lysmata wurdemanni and L. boggessi

  Dong Zhang, Junda Lin, Jörg D. Hardege, Andrew L. Rhyne
  Marine Biology Research 09/2009; 5(5):470-477. · 1.05 Impact Factor
• Article: Biochemical composition of the wild and cultured seahorses, Hippocampus kuda Bleeker and Hippocampus trimaculatus Leach

  Qiang Lin, Junda Lin, Chao Wang
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  ABSTRACT: The biochemical composition of wild and cultured seahorses Hippocampus kuda Bleeker and Hippocampus trimaculatus Leach was analysed in this study. The crude protein contents (% dry weight) of wild seahorses were high: 72.7±2.5% in H. kuda and 78.5±4.2% in H. trimaculatus. The crude lipid contents (% dry weight) of wild and cultured seahorses (1.1±0.1 vs. 1.4±0.2 for H. kuda and 1.2±0.1 vs. 1.2±0.2 for H. trimaculatus) were low and did not differ significantly (F3, 12=1.461, P=0.12). The essential amino acids and flavour-enhancing amino acids in wild seahorses were higher than those in cultured seahorses, although the essential amino acid index in wild and cultured H. kuda and H. trimaculatus were generally low. The total amino acid content decreased with growth in cultured H. trimaculatus (F3, 12=14.927, P<0.05). The poly-unsaturated fatty acid (PUFA) contents in wild and cultured H. kuda and H. trimaculatus were 21.69%, 37.72%, 21.39% and 33.89% respectively. The fatty acid content in seahorses first decreased and then increased with growth both in wild and in cultured H. trimaculatus. The PUFA/saturated fatty acid ratio was 1.31 in cultured H. kuda and 0.87 in cultured H. trimaculatus. The trace metal concentrations in the wild seahorses were relatively low. The results presented in this study provide biochemical information necessary for understanding the medicinal value of H. kuda and H. trimaculatus and for elucidating the potential dietary requirements for their culture.
  Aquaculture Research 03/2009; 40(6):710 - 719. · 1.20 Impact Factor
• Article: MARINE ORNAMENTAL DECAPODS—Popular, Pricey, And Poorly Studied

  Ricardo Calado, Junda Lin, Andrew L. Rhyne, Ricardo Araújo, Luís Narciso
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  ABSTRACT: The growing demand for highly priced marine ornamental species has contributed to the endangered status of coral reefs. A list of 128 of the most heavily traded marine ornamental decapod crustacean species is tabulated. The development of commercial culture techniques, the knowledge of the larval development, and the association with vertebrate and invertebrate organisms are presented for these species. Forty-nine of the species are caridean shrimp, with the Hippolytidae family alone accounting for 15. Anomuran and brachyuran crabs are the next most traded groups (32 and 27 species, respectively), with the pricey stenopodidean shrimp, Astacidea, and Palinura lobsters being represented by a considerably lower number of species (7, 7 and 6, respectively). The main bottlenecks impairing the commercial culture of ornamental shrimp and lobsters are their long larval development and poor survival rates. The main constraint for the development of culture techniques for hermit and brachyuran crabs is their low commercial value. The ecological impacts of harvesting ornamental species are still poorly studied. Nevertheless, the collection in considerable numbers of hermit and small majid crabs (e.g., Clibanarius and Mithraculus) from tidal areas, fish cleaning shrimp (e.g., Lysmata and Stenopus), and the crown-of-thorns sea star eaters Hymenocera, is likely to have serious impacts on the ecosystem. The cooperation between researchers working on larval biology, population dynamics, ecology, aquaculture, and fisheries is essential to properly manage the collection of marine ornamental decapods.
  Journal of Crustacean Biology 01/2009; · 1.12 Impact Factor
• Article: Potential Fertility and Egg Development (Volume, Water, Lipid, and Fatty Acid Content) Through Embryogenesis of Uca rapax (Decapoda: Brachyura: Ocypodidae)

  Joana Figueiredo, Gil Penha-Lopes, Justin Anto, Luís Narciso, Junda Lin
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  ABSTRACT: We examined potential fertility, egg volume, and water, lipid, and fatty acid content through embryogenesis in a population of female U. rapax from Sebastian Inlet, Florida. Carapace width (CW) ranged from 10.80 to 20.09 mm (N = 184), and each female carried 5000 to 30,000 eggs in the last stage of development. Female CW was found to be a good predictor of the number of eggs in the later stage of development (potential fertility = 7.908 CW2.7655, R2 = 0.749). Egg volume increases (from 0.0079 to 0.0134 mm3) was mildly correlated (r = 0.79) with an increase in egg water content (from 60 to 69%). Egg lipid and fatty acid content decreased through embryogenesis, due to its importance as energy source. The most consumed fatty acids were the monounsaturated (97.81 μg . mg dw−1) followed by the saturated (64.34 μg · mg dw−1) and polyunsaturated (38.69 μg · mg dw−1). Fatty acids 16:0, 18:2n-6, 16:1n-7, and 18:2n-6 are consumed preferentially (39.91, 38.45, 29.4 and 23.93 μg · mg dw−1, respectively), while essential polyunsaturated fatty acids, particularly docosahexaenoic acid (22:6n-3) and eicosapentaenoic acid (20:5n-3), are conserved. Egg fatty acid profile also reflects diet and habitat of adults. A medium-low EPA/DHA ratio suggests U. rapax occupies a medium trophic level. The low ratio (18:1n-7/18:1n-9) and high percentages of 18:1n-9 fatty acid (18%) and essential C18 and C20 polyunsaturated fatty acids (19-23%) suggests adults are omnivores consuming primary producers (like algae and mangrove leaves) and small invertebrates. The high percentage of odd-numbered fatty acids (above 3-3.5%) also suggests scavenger/detritivore behaviour.
  Journal of Crustacean Biology 01/2009; · 1.12 Impact Factor
• Chapter: Overview of Marine Ornamental Shrimp Aquaculture

  Ricardo Calado, Luís Narciso, Ricardo Araújo, Junda Lin
  02/2008: pages 219 - 230; , ISBN: 9780470752722
• Article: Efficiency of using emerald crabs Mithraculus sculptus to control bubble alga Ventricaria ventricosa (syn. Valonia ventricosa) in aquaria habitats

  Joana Figueiredo, Luís Narciso, Ralph Turingan, Junda Lin
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  ABSTRACT: Ornamental crabs of the genus Mithraculus (Decapoda: Brachyura: Majidae) are utilized in reef aquaria to control nuisance algae, particularly bubble algae. Although Mithraculus have modified, spooned-shaped chelae to feed on both fleshy and filamentous algae, they may consume alternative foods offered in a reef aquarium. The objective of this study was to determine the efficiency of using Mithraculus sculptus to control the bubble alga Ventricaria ventricosa (Siphonocladales–Cladophorales complex, Chlorophyta) in the presence of alternative foods (commercial pellets and frozen mysids) commonly utilized in reef aquaria. Results indicated that medium and large sized crabs consumed more bubble algae than smaller conspecifics. Although, M. sculptus first chose alternative foods to bubble alga (77% and 69% of the time chose pellets and frozen mysids, respectively), algal consumption only decreased significantly if, besides algae, pellets were provided; when only algae were provided, algal consumption was similar to when they were provided with mysids. The prey choice model was used as a conceptual framework to study the mechanisms underlying active selection; food energy content and handling time were measured and food profitability was calculated. Handling time decreased with increasing crab size. Pellets presented a higher profitability than algae but mysids and algae presented similar profitability; this seems to be in agreement with the observed reduced algal consumption when pellets made part of the diet. Mithraculus sculptus feeding behaviour on V. ventricosa recorded with digital high-speed video (DHSV) suggests that as the crab tears the algal cell apart, the cell liquid that contains juvenile cytoplasmatic spheres is released into the water; this behaviour might contribute to algal dispersal and consequently algal infestation. These results seem to indicate that M. sculptus might not be such an efficient bio-controller of the pest V. ventricosa as previously thought, particularly when pellets are used as food; however, its bio-control efficiency might be improved if, mysids are used as food.
  Journal of the Marine Biological Association of the UK 01/2008; 88(01):95 - 101. · 1.00 Impact Factor
• Article: Effects of Food and Temperature on Survival and Development in the Peppermint Shrimp Lysmata wurdemanni

  Dong Zhang, Junda Lin, R. LeRoy Creswell
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  ABSTRACT: Abstract— The effects of four kinds of foods (Artemia nauplii, rotifer, and microalgae Chaetoceros gracilis and Isochrysis galbana) and four temperature regimes on survival and development of larval Lysmata wurdemanni, a marine ornamental shrimp, were determined. The larvae fed with Chaetoceros or Isochrysis only survived for a maximum of 17 d, before developing to zoea IV. The survivorship of the larvae fed with Artemia nauplii or rotifer from zoea II to post-larvae was 66.7% and 68.9%. respectively, without significant difference (P > 0.05). But larvae fed with Artemia nauplii grew significantly (P < 0.05) faster than those fed with rotifer. Larvae fed with Artemia nauplii reached postlarvae in 29–32 d, compared with 32–36 d in the rotifer treatment. Artemia nauplii are also suitable food for the postlarvae and juveniles of L. wurdemanni. The 30 postlarvae fed on Artemia nauplii all survived to reach sexual maturity in 50 to 70 d, growing from about 7 to 28 mm in total length. The effects of four temperature regimes on larval development of L. wurdemanni was also investigated. The duration (mean ± SD d) to the postlarvae at temperatures of 26 C (37.4 ± 5.4) and of 26–30 C daily (40.2 ± 5.8) was significantly (P < 0.05) longer than that at 28.5 C (29.3 ± 4.8) and at 27–29 C daily (28.7 ± 3.5).
  Journal of the World Aquaculture Society 04/2007; 29(4):471 - 476. · 0.71 Impact Factor
• Article: Effects of density and simulated recruitment and mortality on sex change in a protandric simultaneous hermaphroditic shrimp, Lysmata wurdemanni

  Dong Zhang, Junda Lin
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  ABSTRACT: Sex change in many hermaphrodite animals has been suggested to be environmentally determined, especially socially. To investigate whether sex change in the protandric simultaneous hermaphrodite shrimp Lysmata wurdemanni (Gibbes 1850) is socially mediated, two experiments were conducted in the laboratory between September 2002 and April 2004 using laboratory-cultured shrimp that originated from Port Aransas, Texas, USA. The size at sex change (from male to simultaneous hermaphrodite) in this shrimp is variable, with the minimum around 2.4cm in total length (TL). Large shrimp (2.4–4.5cm TL) still in the male-phase (MP) have been found in the wild and laboratory environments. This study tested the hypothesis that large MP shrimp delay changing to the euhermaphrodite-phase (EP) due to social control. In the first experiment, ten shrimp were raised in large (110-l) and small (20-l) containers to test the effect of habitat size/density on sex change. The percentage of shrimp changing to EP was significantly higher in the large container (low density) than in the small container after 60 and 120days. But after 570days sex ratios were the same, 2MP:8EP. In the second experiment, group composition was changed over time to simulate population recruitment and mortality. MP shrimp delayed sex change when EP shrimp were present. However, if group structure is stable, some MP shrimp may not change sex during their lifetime. Under certain demographic conditions, such as when postlarvae (PL) were added to (simulating recruitment) or EP shrimp were removed from (simulating mortality) a group, all old MP (from original PL) shrimp changed to EP. The response of old MP shrimp to simulated recruitment is faster than to simulated mortality. The present study confirms that social control affects the size and timing of sex change in L. wurdemanni. However, some MP shrimp never change sex suggesting that genetics might also play a role in the sex ratios of L. wurdemanni populations.
  Marine Biology 12/2006; 150(4):639-645. · 2.28 Impact Factor
• Article: A western Atlantic peppermint shrimp complex: redescription of Lysmata wurdemanni, description of four new species, and remarks on Lysmata rathbunae (Crustacea: Decapoda: Hippolytidae)

  Andrew L. Rhyne, Junda Lin
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  ABSTRACT: The present study is the first revision of the western Atlantic peppermint shrimp complex Lysmata wurdemanni (Gibbes). Lysmata wurdemanni, originally described from Florida and South Carolina, is redescribed. Gibbes' types of L. wurdemanni are considered as lost, therefore, a neotype is selected from specimens collected near Key West, Florida. Lysmata rathbunae Chace is rediagnosed; the locality of this species is restricted to Florida and Yucatán; Chace's "variety" of L. rathbunae is separated from L. rathbunae and assigned to a new species. In total, four new species are recognized: Lysmata ankeri n. sp., Lysmata boggessi n. sp., Lysmata pederseni, n. sp. (corresponding to Chace's "variety" of L. rathbunae), and Lysmata bahia n. sp.; all four previously were mistaken for L. wurdemanni and/or L. rathbunae. Classical morphological and morphometrical analyses (Discriminant Function Analysis), in conjunction with examination of life color patterns and interbreeding experiments, were used to corroborate the separation of these cryptic species. Each species may be most easily recognized by the unique color pattern. Lysmata pederseni, n. sp. also differs ecologically in being associated with tube sponges. Lysmata rathbunae appears to be a deep-water species, while the other five species are found mostly in shallow waters.
  Bulletin of Marine Science -Miami- 06/2006; 79(1):165-204. · 1.15 Impact Factor
• Article: Effects of temperature, stocking density and diet on the growth and survival of juvenile Mithraculus forceps (A. Milne Edwards, 1875) (Decapoda: Brachyura: Majidae)

  Gil Penha-Lopes, Andrew L Rhyne, Junda Lin, Luis Narciso
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  ABSTRACT: Mithraculus forceps (A. Milne Edwards) has demonstrated a great potential for ornamental aquaculture and the present study tests the effects of temperature, stocking density and diet on the survival and growth of M. forceps juveniles. For 28 days post metamorphosis (DPM), the newly metamorphosed juveniles were reared at two temperatures (25±0.5 or 28±0.5°C), stocked at five densities (1, 5, 15, 30 or 60 crabs ring−1; approximately 226, 1132, 3395, 6791 or 13 581 crabs m2 respectively) and fed with commercial pellets (CP), microalgae (Amphora spp.), live newly hatched Artemia nauplii (NHA), frozen Artemia nauplii (FNHA), or combinations of each of these diets with NHA. At the end of the temperature experiment, carapace width of the crabs cultured at 28°C was significantly larger than the crabs reared at 25°C and average intermolt period was significantly shorter. Increased stocking density had a negative effect on survivorship and growth. Survivorship at the end of the diet experiment was significantly different between the crabs not fed, fed with CP and Amphora and the crabs fed with the other diets. Between the diet treatments, the crabs fed with NHA+Amphora were significantly larger than the ones fed with NHA+FNHA, NHA, FNHA and NHA+CP, and these in turn larger than ones fed with Amphora.
  Aquaculture Research 02/2006; 37(4):398 - 408. · 1.20 Impact Factor
• Article: The larval rearing of the marine ornamental crab, Mithraculus forceps (A. Milne Edwards, 1875) (Decapoda: Brachyura: Majidae)

  Gil Penha-Lopes, Andrew L. Rhyne, Junda Lin, Luis Narciso
  [show abstract] [hide abstract]
  ABSTRACT: The goal of this study is to develop a larviculture protocol for Mithraculus forceps, a popular marine aquarium species. Different temperatures (25±0.5°C and 28±0.5°C), stocking densities (10, 20, 40 and 80 larvae L−1), prey densities (newly hatched Artemia of 1, 4, 7 and 12 nauplii mL−1) and metamorphosis to crab conditions (Systems A and B) were tested. The best survivorship and faster development were obtained when the larvae were reared at a density of 40 larvae L−1 for 7 days post hatching (DPH) in System A, at 28°C and fed with 7 mL−1 of newly hatched Artemia nauplii. After 7 DPH all the megalopa were moved to System B and the same temperature and prey density were maintained. At the end of the experiment, 12 DPH, survivorship of 74.1±4.8% was obtained.
  Aquaculture Research 08/2005; 36(13):1313 - 1321. · 1.20 Impact Factor
• Article: Development of sexual morphs in two simultaneous hermaphroditic shrimp, Lysmata wurdemanni and Lysmata rathbunae

  DONG ZHANG, JUNDA LIN
  Invertebrate Reproduction and Development 01/2005; 47(1):11-17. · 0.48 Impact Factor
• Article: Effects of different diets on larval development in a peppermint shrimp (Lysmata sp. (Risso))

  Andrew L Rhyne, Junda Lin
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  ABSTRACT: Currently, only a handful of marine ornamental species are commercially produced through aquaculture. In order for the marine aquarium industry to continue to grow, a diverse selection of cultured animals is required to offset wild collections. Long and variable larval durations are the major bottleneck for mass production of marine ornamental shrimp. Improving larval diet may reduce the larval duration and enhance the prospects of commercial aquaculture. A laboratory study was conducted to examine the effect of different diets on larval development and survival of the peppermint shrimp, Lysmata (gulf coast variety), a popular aquarium shrimp. The effect of three feeding regimes [Artemia alone (ART), ArteMac™ alone (COMM) and Artemia in combination with ArteMac™ (ART/COMM)] on survival and development were tested. Survival to zoea 5 for both ART and ART/COMM treatments was 99%, but only 62.5% for COMM treatment. ArteMac™ alone treatment shrimp only survived to zoea 7. Survival to postlarvae for ART treatment (72.5%) was significantly lower (P<0.01) than those of treatment ART/COMM (80.5%). Larvae fed ART/COMM had significantly (P<0.01) shorter larval duration than larvae fed ART. Fourteen days after the first postlarva appeared, the rates of settlement (85.4% and 67.5% respectively) and survival (68.8% and 49.0% respectively) were significantly greater (P<0.01) for larvae fed the ART/COMM than those fed ART.
  Aquaculture Research 09/2004; 35(12):1179 - 1185. · 1.20 Impact Factor