Katarzyna Gurzawska

Publications (2)6.84 Total impact

• Article: Nanocoating of titanium implant surfaces with organic molecules. Polysaccharides including glycosaminoglycans.

  Katarzyna Gurzawska, Rikke Svava, Niklas Rye Jørgensen, Klaus Gotfredsen
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  ABSTRACT: Long-term stability of titanium implants are dependent on a variety of factors. Nanocoating with organic molecules is one of the method used to improve osseointegration. Nanoscale modification of titanium implants affects surface properties, such as hydrophilicity, biochemical bonding capacity and roughness. This influences cell behaviour on the surface such as adhesion, proliferation and differentiation of cells as well as the mineralization of the extracellular matrix at the implant surfaces. The aim of the present systematic review was to describe organic molecules used for surface nanocoating with focus on polysaccharides including glycosaminoglycans, and how these molecules change surface properties, cell reactions and affect on osseointegartion. The included in vitro studies demonstrated increased cell adhesion, proliferation and mineralization of a number of the tested polysaccharide nanocoatings. The included in vivo studies, showed improvement of bone interface reactions measured as increased Bone-to-Implant Contact length and Bone Mineral Density adjacent to the polysaccharide coated surfaces. Based on existing literature, surface modification with polysaccharide and glycosaminoglycans appears to be an effective way to stimulate bone regeneration on bone-implant interface.
  Journal of Biomedical Nanotechnology 12/2012; 8(6):1012-24. · 4.22 Impact Factor
• Article: Effect of nanocoating with rhamnogalacturonan-I on surface properties and osteoblasts response.

  Katarzyna Gurzawska, Rikke Svava, Susanne Syberg, Yu Yihua, Kenneth Brian Haugshøj, Iben Damager, Peter Ulvskov, Leif Højslet Christensen, Klaus Gotfredsen, Niklas Rye Jørgensen
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  ABSTRACT: Long-term stability of titanium implants are dependent on a variety of factors. Nanocoating with organic molecules is one of the methods used to improve osseointegration. Therefore, the aim of this study is to evaluate the in vitro effect of nanocoating with pectic rhamnogalacturonan-I (RG-I) on surface properties and osteoblasts response. Three different RG-Is from apple and lupin pectins were modified and coated on amino-functionalized tissue culture polystyrene plates (aminated TCPS). Surface properties were evaluated by scanning electron microscopy, contact angle measurement, atomic force microscopy, and X-ray photoelectron spectroscopy. The effects of nanocoating on proliferation, matrix formation and mineralization, and expression of genes (real-time PCR) related to osteoblast differentiation and activity were tested using human osteoblast-like SaOS-2 cells. It was shown that RG-I coatings affected the surface properties. All three RG-I induced bone matrix formation and mineralization, which was also supported by the finding that gene expression levels of alkaline phosphatase, osteocalcin, and collagen type-1 were increased in cells cultured on the RG-I coated surface, indicating a more differentiated osteoblastic phenotype. This makes RG-I coating a promising and novel candidate for nanocoatings of implants.
  Journal of Biomedical Materials Research Part A 12/2011; 100(3):654-64. · 2.63 Impact Factor