Alice Delbianco

University of Bologna, Bolonia, Emilia-Romagna, Italy

Are you Alice Delbianco?

Claim your profile

Publications (4)11.54 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Agroinoculation is a quick and easy method for the infection of plants with viruses. This method involves the infiltration of tissue with a suspension of Agrobacterium tumefaciens carrying binary plasmids harbouring full-length cDNA copies of viral genome components. When transferred into host cells, transcription of the cDNA produces RNA copies of the viral genome that initiate infection. We produced full-length cDNA corresponding to Beet necrotic yellow vein virus (BNYVV) RNAs and derived replicon vectors expressing viral and fluorescent proteins in pJL89 binary plasmid under the control of the Cauliflower mosaic virus 35S promoter. We infected Nicotiana benthamiana and Beta macrocarpa plants with BNYVV by leaf agroinfiltration of combinations of agrobacteria carrying full-length cDNA clones of BNYVV RNAs. We validated the ability of agroclones to reproduce a complete viral cycle, from replication to cell-to-cell and systemic movement and, finally, plant-to-plant transmission by its plasmodiophorid vector. We also showed successful root agroinfection of B. vulgaris, a new tool for the assay of resistance to rhizomania, the sugar beet disease caused by BNYVV.
    Molecular Plant Pathology 02/2013; · 3.88 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The RNA silencing suppression properties of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV) cysteine rich protein p14s have been investigated. Suppression of RNA silencing activities were evidenced using viral infection of silenced Nicotiana benthamina 16C, N. benthamina agroinfiltrated with GFP and GF-FG triggers supplemented with viral suppressors of RNA silencing (VSR) constructs or using complementation of a silencing suppressor defective BNYVV virus in Chenopodium quinoa. Northern blot analyses of siRNAs in agroinfiltration tests revealed reduced amounts of siRNA, especially secondary siRNA, suggesting that benyvirus VSRs act downstream of the siRNA production. Using confocal laser scanning microscopy imaging of infected protoplasts expressing functional p14 protein fused to an EGFP reporter, we showed that benyvirus p14 accumulated in the nucleolus and the cytoplasm independently of other viral factors. Site directed mutagenesis showed the importance of the nucleolar localization signal (NoLS) embedded in a C4 zinc-finger domain in the VSR function and intrinsic stability of the p14 protein. Conversely RNA silencing suppression appeared independent of the nucleolar localization of the protein and a correlation between BNYVV VSR expression and long distance movement was established.
    Molecular Plant-Microbe Interactions 09/2012; · 4.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cited By (since 1996):4, Export Date: 15 January 2014, Source: Scopus, CODEN: VIRLA, doi: 10.1016/j.virol.2011.12.007, PubMed ID: 22209119, Language of Original Document: English, Correspondence Address: Ratti, C.; University of Bologna, DiSTA - Plant Patology, Viale G. Fanin 40, 40127 Bologna (BO), Italy; email: claudio.ratti@unibo.it, Molecular Sequence Numbers: GENBANK: EU410955, FJ424610, JF513084, JF693322, NC_003508;, Chemicals/CAS: Viral Proteins, References: Bleykasten-Grosshans, C., Guilley, H., Bouzoubaa, S., Richards, K.E., Jonard, G., Independent expression of the first two triple gene block proteins of beet necrotic yellow vein virus complements virus defective in the corresponding gene but expression of the third protein inhibits viral cell-to-cell movement (1997) Mol. Plant Microbe Interact., 10, pp. 240-246;
    Virology. 01/2012; 423(2):187-194.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Beet soil-borne mosaic virus (BSBMV), like Beet necrotic yellow vein virus (BNYVV), is a member of the Benyvirus genus and both are transmitted by Polymyxa betae. Both viruses possess a similar genomic organization: RNA-1 and -2 are essential for infection and replication while RNA-3 and -4 play important roles in disease development and vector-mediated infection in sugar beet roots. We characterized a new species of BSBMV RNA-4 that encodes a 32 kDa protein and a chimeric form of BSBMV RNA-3 and -4. We demonstrated that BSBMV RNA-4 can be amplified by BNYVV RNA-1 and -2 in planta, is involved in symptoms expression on Chenopodium quinoa plants and can also complement BNYVV RNA-4 for virus transmission through its vector P. betae in Beta vulgaris plants. Using replicon-mediated expression, we demonstrate for the first time that a correct expression of RNAs-4 encoded proteins is essential for benyvirus transmission.
    Virology 12/2011; 423(2):187-94. · 3.35 Impact Factor