Lei Fan

Beijing Institute Of Technology, Peping, Beijing, China

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Publications (2)2.11 Total impact

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    ABSTRACT: A Gram-staining-positive bacterium, designated strain XN13T, was isolated from a soil sample collected from ALaShan national geological park in Inner Mongolia Autonomous Region, China and subjected to a polyphasic taxonomic study. Strain XN13T was found to have a range of chemical and morphological properties consistent with its classification in the genus Planomicrobium. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain XN13T was related to members of the genus Planomicrobium. The closest phylogenetic relatives were Planomicrobium okeanokoites IFO 12536T, Planomicrobium koreense JG07T, Planomicrobium mcmeekinii S23F2T and Planomicrobium flavidum ISL-41T with 98.2%, 97.8%, 97.8%, 97.7% sequence similarity, respectively. The major cellular fatty acids were anteiso-C15:0, C16:1ω7c alcohol, iso-C14:0 and C16:1ω11c. The predominant menaquinones were MK-8 and MK-7. The DNA G+C content was 40.3%. The level of DNA-DNA relatedness between strain XN13T and Planomicrobium okeanokoites KCTC 3672T, Planomicrobium koreense KCTC 3684T, Planomicrobium mcmeekinii CGMCC 1.2724T, Planomicrobium flavidum KCTC 13261T, Planomicrobium chinese CGMCC 1.3454T and Planomicrobium glaciei CGMCC 1.6846T were 36%, 30%, 34%, 29%, 30% and 31%, respectively. The organism is different from Planomicrobium species in several phenotypic characteristics. On the basis of phenotypic and genotypic properties, strain XN13T represents a novel species of the genus Planomicrobium, for which the name Planomicrobium soli sp. nov., is proposed. The type strain is XN13T (= CGMCC 1.12259T = KCTC 33047T).
    International journal of systematic and evolutionary microbiology. 05/2014;
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    ABSTRACT: A Gram-positive-staining bacterium, designated Y16(T), was isolated from a soil sample from Yunnan Province, China. The isolate grew optimally at 25-30 °C, grew at pH 6.0-9.0 and could grow with 3 % NaCl. Strain Y16(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinones were MK-9(H(4)) and MK-9(H(2)). The major fatty acid methyl esters were anteiso-C(15 : 0), iso-C(15 : 0), anteiso-C(17 : 0) and C(16 : 0). These chemotaxonomic characteristics suggested that the organism belonged to the genus Actinoplanes. Strain Y16(T) shared 98.7, 98.3 and 97.9 % 16S rRNA gene sequence similarity with Actinoplanes deccanensis IFO 13994(T), A. abujensis A4029(T) and A. brasiliensis DSM 43805(T), respectively. The DNA G+C content of the isolate was 70.8 mol%. DNA-DNA relatedness between the novel isolate and the type strains of A. deccanensis, A. abujensis and A. brasiliensis was 35.2, 32.0 and 22.3 %, respectively. In addition, the pattern of phenotypic properties distinguished strain Y16(T) from its closest phylogenetic neighbours. It is therefore concluded that strain Y16(T) ( = CGMCC 4.6857(T) = JCM 17700(T)) represents a novel species of the genus Actinoplanes, for which the name Actinoplanes atraurantiacus sp. nov. is proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; 62(Pt 10):2533-7. · 2.11 Impact Factor