Publications (3)4.57 Total impact
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Article: Microencapsulation of Bifidobacterium bifidum F-35 in Whey Protein-Based Microcapsules by Transglutaminase-Induced Gelation.
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ABSTRACT: Bifidobacterium bifidum F-35 was microencapsulated into whey protein microcapsules (WPMs) by a transglutaminase (TGase)-induced method after optimization of gelation conditions. The performance of these WPMs was compared with that produced by a spray drying method (WPMs-A). WPMs produced by the TGase-induced gelation method (WPMs-B) had larger and denser structures in morphological examinations. Native gel and SDS-PAGE analyses showed that most of the polymerization observed in WPMs-B was due to stable covalent crosslinks catalyzed by TGase. The degradation properties of these WPMs were investigated in simulated gastric juice (SGJ) with or without pepsin. In the presence of pepsin, WPMs-A degraded more quickly than did WPMs-B. Finally, survival rates of the microencapsulated cells in both WPMs were significantly better than that of free cells and varied with the microencapsulation method. However, WPMs-B produced by TGase-induced gelation could provide better protection for microencapsulated cells in low pH conditions and during 1 mo of storage at 4 °C or at ambient temperature.Journal of Food Science 05/2012; 77(5):M270-7. · 1.66 Impact Factor -
Article: Antagonistic activities of lactobacilli against Helicobacter pylori growth and infection in human gastric epithelial cells.
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ABSTRACT: Lactobacilli have positive effects on bowel microflora and health in humans and animals. In this study, the antagonistic activities of Lactobacillus gasseri Chen, and L. plantarum 18 were assessed by agar plate diffusion assay and tests that determined the growth and urease activity of Helicobacter pylori cocultured with lactobacilli and the adherence of H. pylori to human gastric epithelial cells in the presence of lactobacilli. The results showed that the 2 Lactobacillus strains had significant anti-H.pylori activity, and this activity may be contributed by the cell-free supernatants (CFS) of lactobacilli and live Lactobacillus strains in vitro. The antagonistic activity of the CFS against H. pylori depended on the pH and the presence of metabolites, such as organic acids and proteases. Our results also indicated that 2 Lactobacillus strains could inhibit H. pylori adherence human gastric epithelial cells. PRACTICAL APPLICATION: Helicobacter pylori causes chronic gastritis, peptic ulcer disease, and gastric cancer, and it infects about 50% of the world's population. Lactobacilli have been reported to have an inhibitory effect on H. pylori and can be used as probiotic to manufacture dairy products preventing H. pylori infection.Journal of Food Science 12/2011; 77(1):M9-14. · 1.66 Impact Factor -
Article: Microencapsulation of Bifidobacterium bifidum F‐35 in reinforced alginate microspheres prepared by emulsification/internal gelation
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ABSTRACT: Alginate microspheres containing Bifidobacterium bifidum F-35 prepared by emulsification/internal gelation were reinforced by blending with pectin or starch or coating with chitosan or poly-L-lysine to provide extra protection for the strain. The influence of these treatments on the size of microspheres, encapsulation yield (EY) and protective effect of microencapsulation on the cells was studied. No difference was detected in EY with different types of reinforcement, which was approximately 43–50%. The mean diameter of reinforced alginate microspheres ranged from 117 to 178 μm, reaching a maximum value when starch was incorporated in the alginate matrix. It was observed that the protective effects varied with the type of reinforcement. However, chitosan-coated alginate microspheres provided the best protection for microencapsulated cells in simulated gastrointestinal tract and during 1 month of storage at 4 °C, and this system could be the comparatively effective vector of bifidobacteria for intestinal delivery.International Journal of Food Science & Technology 06/2011; 46(8):1672 - 1678. · 1.26 Impact Factor
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Institutions
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2011
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Jiangnan University
- School of Food Science and Technology
Wuxi, Jiangsu Sheng, China -
Inner Mongolia Agricultural University
Hohhot, Inner Mongolia, China
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