Benshu Zhang

Tianjin Medical University, T’ien-ching-shih, Tianjin Shi, China

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Publications (6)11.59 Total impact

  • Ying Liu, Meiyu Peng, Dawei Zang, Benshu Zhang
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    ABSTRACT: To investigate the roles of leukemia inhibitory factor (LIF) in a 6-hydroxydopamine (6-OHDA) mouse model of Parkinson disease (PD), and explore how the LIF improves PD symptoms. This study was performed in Tianjin Medical University, Tianjin, China, between April 2008 and January 2010. Seventy-two mice were allocated into a control group (CON), sham operation group (SHA), physiological saline (NS) treatment group (PD), and LIF treatment group (LIF), n=18 for each group. The 6-OHDA was injected into the mice`s left mid-striatum to build a 6-OHDA model of PD. The LIF or NS was slowly released into the CSF through the ALZET osmotic pump catheters duct, in the LIF or NS treated groups. The whole treatment lasted 3 weeks, and the motor functions of the mice were assessed on the seventh, fourteenth, and twenty-first day during the treatment. The nestin-positive cells in the mice were counted by immunofluorescence assays, and the level of gp130 was detected with western blot analysis. After CSF infusion in the LIF-treated group, we observed an increased number of nestin-positive cells in the mice`s brains. The expression of a major component of the LIF receptor complex, gp130, was also increased. In the fourteenth and twenty-first day time periods; LIF treatment continuously improved the motor functions of the mouse model of PD. These results suggest the potential of LIF administration as a therapeutic method for PD.
    Neurosciences 10/2013; 18(4):363-70. · 0.32 Impact Factor
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    ABSTRACT: Dopa-responsive dystonia (DRD) is a rare inherited dystonia that responds very well to levodopa treatment. Genetic mutations of GTP cyclohydrolase I (GCH1) or tyrosine hydroxylase (TH) are disease-causing mutations in DRD. To evaluate the genotype-phenotype correlations and diagnostic values of GCH1 and TH mutation screening in DRD patients, we carried out a combined study of familial and sporadic cases in Chinese Han subjects. We collected 23 subjects, 8 patients with DRD, 5 unaffected family members, and 10 sporadic cases. We used PCR to sequence all exons and splicing sites of the GCH1 and TH genes. Three novel heterozygous GCH1 mutations (Tyr75Cys, Ala98Val, and Ile135Thr) were identified in three DRD pedigrees. We failed to identify any GCH1 or TH mutation in two affected sisters. Three symptom-free male GCH1 mutation carriers were found in two DRD pedigrees. For those DRD siblings that shared the same GCH1 mutation, symptoms and age of onset varied. In 10 sporadic cases, only two heterozygous TH mutations (Ser19Cys and Gly397Arg) were found in two subjects with unknown pathogenicity. No GCH1 and TH mutation was found in 40 unrelated normal Han Chinese controls. GCH1 mutation is the main etiology of familial DRD. Three novel GCH1 mutations were identified in this study. Genetic heterogeneity and incomplete penetrance were quite common in DRD patients, especially in sporadic cases. Genetic screening may help establish the diagnosis of DRD; however, a negative GCH1 and TH mutation test would not exclude the diagnosis.
    PLoS ONE 01/2013; 8(6):e65215. · 3.53 Impact Factor
  • Ping Zhao, Benshu Zhang, Shuo Gao
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    ABSTRACT: To investigate the difference in glucose metabolism on (18)F-fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) imaging for differential diagnosis of idiopathic Parkinson's disease (IPD) from several parkinsonian-plus syndromes using SPM2 approach. (18)F-FDG PET was performed for 18 IPD patients, 22 multiple system atrophy (MSA) patients, 13 progressive supranuclear palsy (PSP) patients, 5 corticobasal degeneration (CBD) patients, 7 dementia with Lewy bodies (DLB) patients and 1 normal pressure hydrocephalus (NPH) patient. Imaging-based diagnosis was obtained by statistical parametric mapping (SPM2) software to analyze the differences and overlaps among these groups. The (18)F-FDG PET images analyzed with SPM2 demonstrated that a reduction in glucose metabolism occurred in bilateral parietal area for IPD, in bilateral putamen for MSA-P, in bilateral cerebellum for MSA-C, in midbrain and the middle frontal cortex for PSP, in asymmetrical metabolism of the cortex and the basal ganglia for CBD, in bilateral occipital and parieto-occipital areas for DLB. The metabolic reductions in a patient of NPH group were observed in the ventricular system. This study identifies different patterns of glucose metabolism in parkinsonism. (18)F-FDG PET imaging may contribute to early differential diagnosis in clinically ambiguous cases of parkinsonism.
    Parkinsonism & Related Disorders 01/2012; 18 Suppl 1:S60-2. · 3.27 Impact Factor
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    ABSTRACT: The key pathological feature of Parkinson's disease (PD) is selective degeneration of the neuromelanin (NM)-pigmented dopaminergic neurons in the substantia nigra (SN). NM, like other risk factors, such as oxidative stress (OS) and α-synuclein (α-syn), is involved in the pathogenesis of PD. But whether or not NM synergizes with α-syn or OS in the pathogenesis of PD remains unexplored. In the present study, we examined the effects of NM on cellular viability, apoptosis and free radical production in α-syn over-expressing human neuroblastoma cell line (SK-N-SH) in the presence or absence of the oxidizer Fenton's Reagent (FR). We showed that NM synergized with FR in suppressing cell viability, and in inducing apoptosis and hydroxyl radical production in all SK-N-SH cell lines. α-Syn over-expressing cells exhibited more pronounced effect, especially the A53T mutation. Our findings suggest that NM synergizes with both OS and α-syn in conferring dopaminergic vulnerability, adding to our understanding of the pathogenesis of PD.
    Journal of Neural Transmission 12/2011; 119(6):685-91. · 3.05 Impact Factor
  • Haijuan Yan, Benshu Zhang
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    ABSTRACT: A simple hydrothermal method was developed to prepare monodispersed and well-crystallized hematite nanoparticles with a diameter distribution of about 180nm. In-vitro cytotoxicity of such nanoparticles was carried out using Hek 293 cell culture system with different dosages. Assessment of cell viability reveals that hematite nanoparticles reduce cell viability in a dose- and time-dependent manner within a rather wide dosage range. Such cytotoxicity is correlative to the decrease of the activity of antioxidative enzymes induced by the oxidative stress in cells.
    Materials Letters - MATER LETT. 01/2011; 65(5):815-817.
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    ABSTRACT: Clinical evidence indicates that the Tourette's syndrome (TS) is associated with hyperactivity of the dopaminergic system; however, imaging studies of dopamine transporter (DAT) in TS patients remain controversial. In this study, we aimed to study DAT binding capacities in a relatively larger sample of drug-naive patients with TS in comparison with controlled subjects by (99m)Tc-TRODAT-1 SPECT/CT imaging. We also aimed to look for any possible correlations between DAT and age, disease duration or tic severity of TS, which have not been thoroughly investigated in previous studies. We tried to provide more evidence for the understanding of the physiopathological mechanism of TS from the molecular imaging perspective. Eighteen drug-naive patients with TS and 8 age- and gender-matched healthy subjects were recruited. Severity of TS was measured with Yale Global Tic Severity Scale. Brain SPECT/CT was performed 2.5 h after injection of (99m)Tc-TRODAT-1. Regions of interest were drawn on the striatum including its sub-regions of caudate and putamen. The cerebellum was used as the reference region. DAT uptake ratio was calculated by subtracting the mean counts per pixel in the cerebellum from the mean counts per pixel in the striatum, caudate or putamen and by dividing the result by the mean counts per pixel in the cerebellum. Comparisons of DAT uptake ratios between TS patients and controls, and comparisons in bilateral striatum and sub-regions in TS patients were carried out. Correlation analysis between DAT uptake ratios and clinical data were also conducted. TS patients showed significantly higher uptake of (99m)Tc-TRODAT-1 in bilateral striatum in comparison with the controls. There was no group-specific preferential lateralization in striatal uptake. DAT uptake ratios were not correlated with age and tic severity scores, but significant negative correlation with disease duration was found. High level of DAT was demonstrated in drug-naive TS patients compared with normal controls, and it was negatively correlated with TS duration. Therefore, with the extension of the disease duration, certain degree of adaptation might occur in TS patients to compensate for the DAT increase, which suggested that hyper-functional DAT might be involved in the early pathophysiological changes of TS.
    Annals of Nuclear Medicine 08/2010; 24(7):515-21. · 1.41 Impact Factor