Marana Park

Seoul National University, Sŏul, Seoul, South Korea

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Publications (8)4.49 Total impact

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    ABSTRACT: White-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is known as a major rice and long-range migratory pest in Asia. Microsatellite marker (SSR) is widely used to perceive the origins and genetic diversity of insect pest. Samples were collected from Laos, Vietnam and 3 localities in Bangladesh, and we characterized novel 12 microsatellite loci of S. furcifera by using next-generation Roche 454 pyrosequencing technologies. We used 40 adult individuals collected from Shinan to test utility of ten microsatellite loci. The average of alleles per locus were 7.92. The mean of observed (HO) and expected heterozygosities (HE) were 0.615 and 0.757 respectively. These new microsatellite markers will be widely used in future ecological genetic studies of S. furcifera, including gene flow and genetic diversity among the population that are necessary for effective management and observing of the species. Furthermore, we used these markers to determine genetic diversity for S. furcifera specimens collected from Korea (Shinan), Laos, Nepal, Thailand, Vietnam and four different sites of Bangladesh in 2012, and China, Nepal ,Thailand, two different sites of Bangladesh, and fifteen different sites of Korea in 2013. The genetic variability estimates for each S. furcifera population infer from the ten microsatellite loci included the observed (HO) and expected (HE) heterozygosity and the inbreeding coefficient. HO ranged from 0.275 – 0.615 (mean = 0.441) and HE ranged from 0.574 - 0.790 (mean = 0.671). The FIS ranged from 0.0467 in Vietnam to 0.5855 in Jindo (Korea), with mean of 0.3554 across loci. In genetic variability estimates for each S. furcifera population in Asia, inferred from twelve microsatellite markers, Shinan has a highest number of HO 0.615. This result shows the possibility of variation of the migration source. We speculate Vietnam as the origin of S. furcifera in Korea by the low number of FIS and high number of HO. The genetic variability estimates for each S. furcifera population in Korea, Changnyung, Gosung, Milyang, Shinan and Taean show high estimation of HO. All of these sites located in south and southeast part of Korea and may assume variability of migration origin. This study provides useful data to forecast the migration and origin of S. furcifera. This information about migration pattern may develop sustainable pest management strategies of this long-range migratory pest.
    Entomological Society of America Annual Meeting 2014; 11/2014
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    ABSTRACT: The Small brown planthopper (Laodelphax striatellus Falln, SBPH) is serious migration pest, damaged to paddy field, in Asia regions. The source of migration was known to China. In South Korea, regular migration has been mainly western parts in early June(or late May). Also, it overwintered in paddy field. Under migration history and density, genetic structure was expected to differentiation between overwintering and migration populations SBPH in South Korea. SBPH was collected 27 samples (2 time in same site) in paddy field using the beating and sweeping methods in 2013. Sampling timing was divided into April (overwintering populations) and September (3~4 generations after overwintering). Extracted 1,091 DNA samples were genotyped by performing the multiplex PCR using nine microsatellite markers. Therefore, we compared the genetic structure of SBPH among/between overwintering and migration populations. The expected heterozygosities (HE) and observed heterozygosities (HO) ranged from 0.772 to 0.846 and 0.391 to 0.571, respectively. Genetic differentiation(FST) ranged from -0.0064 to 0.0231. IBD (Isolation By Distance) showed not significant in all populations (p=0.3, r² =0.0015) indicating the high gene flow and/or recent range expansion. This results will be used important information for migration pest management of SBPH in South Korea. Further, we discuss the relationship between RSV(Rice Stripe Virus) and SBPH on the dispersal abilities.
    Entomological Society of America Annual Meeting 2014; 11/2014
  • Marana Park, Kyung-Seok Kim, Joon-Ho Lee
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    ABSTRACT: Lycorma delicatula (White) was identified in 2004 as an invasive pest in South Korea, where it causes serious damage to vineyard crops. To investigate the population structure and dispersal pattern of L. delicatula in South Korea, we estimated the population genetic structure and gene flow among nine locations across the country using seven microsatellite markers. Although L. delicatula spread throughout most of its geographical range in South Korea within 5-7 years following invasion, its populations show evidence of genetic structuring across the range with a low but significant global F ST (genetic differentiation across all populations) of 0.0474. Bayesian-based clustering analysis indicates the presence of at least three genetically unique populations in South Korea, including populations in northeastern South Korea, which show a distinct genetic background. However, isolation by distance suggests that populations in South Korea have not yet reached genetic equilibrium. Estimates of the historical rate of gene flow (N e m) indicate that relatively high rates of flow have been maintained among populations within the western region, which may indicate recent range expansion. A population assignment test using the first-generation migrant detection method suggested that long-distance dispersal of L. delicatula may have occurred over large areas of South Korea. More complex dispersal patterns may have occurred during L. delicatula invasion of heterogeneous landscapes in South Korea.
    Bulletin of entomological research 03/2013; · 1.99 Impact Factor
  • Marana Park, Joon-Ho Lee
    Entomological Society of America Annual Meeting 2012; 11/2012
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    ABSTRACT: Lycorma delicatula is invasive pest, native to China, in Korea. It has rapid spread throughout South Korea where the species causes crop damage, especially vineyard crops. We studied the genetic variability and structure of four populations of L. delicatula between 2010 and 2012 in Korea. From sample location and time were genotyped at seven microsatellite loci. In total, 80 alleles were detected across the 7 microsatellite loci. The number of alleles was 6.32 and 5.29 in 2010 and 2011, respectively. Pairwise FST estimates ranged from 0.022 (GJ-2012 and DG-2012) to 0.071 (CA-2010 and GJ-2012). Genetic differentiation between each pair population indicated the significant, except for SW and GJ population both 2010 and 2012. In principal coordinate (PCo) analysis, the mean factor scores were plotted along the first principal component axes, which accounted for 33.47% of the total variance. The value of K calculated from Ln P(D) of the STRUCTURE output revealed a maximum value of 16.19 at K = 2 among the genotypes. In this study, geographical spread of L. delicatula in South Korea were discussed through genetic profiles.
    Entomological Society of America Annual Meeting 2012; 11/2012
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    ABSTRACT: Frankliniella occidentalis (Pergande), a vector of tomato spotted wilt virus (TSWV), has spread nationwide with damage to vegetables and floricultural crops in South Korea. A population model was developed for F. occidentalis in pepper fields. The model was structured by the three developmental stages of F. occidentalis egg, larva-pupa, adult and the parameter values were estimated from the laboratory experiment data. The basic components of the model are stage-specific development models and oviposition model, and mortality factors were incorporated. The population model predicted timing of the first occurrence of F. occidentalis well. The model was validated using data for adults’ density in pepper fields in South Korea. The population model of F. occidentalis developed in this study will be useful to predict its seasonal population dynamics in pepper fields.
    Entomological Society of America Annual Meeting 2012; 11/2012
  • Marana Park, Kyung-Seok Kim, Joon-Ho Lee
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    ABSTRACT: Lycorma delicatula (White) is native to China but is becoming an important insect pest in Korea. Polymorphic DNA markers like microsatellites are widely used for characterizing dispersal patterns and capacity of invasive insect pests which can contribute to designing effective management of the species. To facilitate such population genetic studies of L. delicatula in Korea, we isolated and characterized eight microsatellite loci for L. delicatula using a hybridization-biotin enrichment method. We further used these novel microsatellite loci to determine population genetic parameters for 33 L. delicatula specimens collected from Cheonan, South Korea where outbreaks of this species were first reported in Korea. The number of alleles per locus ranged from three to ten, with an average of 6.25. The mean expected (H (E)) and observed heterozygosities (H (O)) were 0.575 and 0.626, respectively. The eight loci showed no deviation from Hardy-Weinberg equilibrium according to the adjusted significance threshold (P = 0.00625), and there was no linkage disequilibrium between each pair of these eight markers. Bayesian cluster analysis using the program structure revealed no evidence of genetic structuring in L. delicatula samples from Cheonan. These new microsatellite markers will be widely applicable to future ecological genetic studies of L. delicatula, including assessment of the level of gene flow and genetic connectivity among populations that are necessary for effective management and monitoring of the species.
    Molecular Biology Reports 12/2011; 39(5):5637-41. · 2.51 Impact Factor
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    ABSTRACT: Lycorma delicatula (Hemiptera: Fulgoridae) is considered an invasive pest of grape vine in Korea. This study was conducted to develop a forecasting model to predict the spring hatch of overwintered eggs of Lycorma delicatula(White).We collected overwintering egg masses in the major grape growing area on 1st and 17th February, and 4th March in 2010. For eggs collected on respective sampling date, they were chilled at 5C for 15 days, and them development was investigated 15, 19, 23, 27, 31, and 35C and a photoperiod of 16:8(L:D)h. For each treatment, egg numbers range from 14 to 27. The hatching rate of eggs was highest at 15C (92.088.72%) followed by 19C (90.1218.89%), 23C (87.8819.32%), 27C (75.9624.82%) and 31C (30.9224.81%). Eggs did not survive at 35C. The developmental duration (MeanSD) of eggs was 69.196.23, 39.472.24, 22.963.25, 17.561.58 and 12.156.29 days, at 15, 19, 23, 27 and 31C, respectively. The relationship between egg developmental rate and temperature was described by a linear model (Y=0.0034X-0.036 (r2=0.988)),and a non-linear model of Gaussian equation (r2=0.998). The developmental variation of eggs was described by the three-parameter Weibull function (r2=0.958). The lower developmental threshold temperature was 10.73C and effective accumulated temperature for egg development was 297.62 Degree days. The degree-day model using sine wave and the rate summation model were validated using field observation data for occurrence of the 1stinstar nymph. Both models predicted actual observed date for 50% hatch of overwintered egg masses very well. Key words: Lycorma delicatula, egg development, forecasting model
    Entomological Society of America Annual Meeting 2010; 12/2010