[Show abstract][Hide abstract] ABSTRACT: Aberrant interactions between the host and the intestinal bacteria are thought to contribute to the pathogenesis of many digestive diseases. However, studying the complex ecosystem at the human mucosal-luminal interface (MLI) is challenging and requires an integrative systems biology approach. Therefore, we developed a novel method integrating lavage sampling of the human mucosal surface, high-throughput proteomics, and a unique suite of bioinformatic and statistical analyses. Shotgun proteomic analysis of secreted proteins recovered from the MLI confirmed the presence of both human and bacterial components. To profile the MLI metaproteome, we collected 205 mucosal lavage samples from 38 healthy subjects, and subjected them to high-throughput proteomics. The spectral data were subjected to a rigorous data processing pipeline to optimize suitability for quantitation and analysis, and then were evaluated using a set of biostatistical tools. Compared to the mucosal transcriptome, the MLI metaproteome was enriched for extracellular proteins involved in response to stimulus and immune system processes. Analysis of the metaproteome revealed significant individual-related as well as anatomic region-related (biogeographic) features. Quantitative shotgun proteomics established the identity and confirmed the biogeographic association of 49 proteins (including 3 functional protein networks) demarcating the proximal and distal colon. This robust and integrated proteomic approach is thus effective for identifying functional features of the human mucosal ecosystem, and a fresh understanding of the basic biology and disease processes at the MLI.
PLoS ONE 01/2011; 6(11):e26542. · 3.73 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: If plastic surgeons are to provide state-of-the-art care in the techniques of skin rejuvenation and minimally invasive treatments
for aging, a thorough understanding of skin, anatomy, and physiology is required. With the changing population demographics,
the plastic surgeon must also be knowledgeable about ethnic variations in skin anatomy and physiology. The changes that occur
with aging skin, inherent developmental disorders, and development of skin cancers are complex. This chapter provides a practical
guide to the understanding of skin and its application to clinical conditions.