Seychelle M Vos

Publications of Seychelle M Vos

• All tangled up: how cells direct, manage and exploit topoisomerase function.

  Authors: Seychelle M Vos, Elsa M Tretter, Bryan H Schmidt, James M Berger

  Nature reviews. Molecular cell biology. 01/2011; 12(12):827-41.

  Topoisomerases are complex molecular machines that modulate DNA topology to maintain chromosome superstructure and integrity. Although capable of stand-alone activity in vitro, topoisomerases areTopoisomerases are complex molecular machines that modulate DNA topology to maintain chromosome superstructure and integrity. Although capable of stand-alone activity in vitro, topoisomerases are frequently linked to larger pathways and systems that resolve specific DNA superstructures and intermediates arising from cellular processes such as DNA repair, transcription, replication and chromosome compaction. Topoisomerase activity is indispensible to cells, but requires the transient breakage of DNA strands. This property has been exploited, often for significant clinical benefit, by various exogenous agents that interfere with cell proliferation. Despite decades of study, surprising findings involving topoisomerases continue to emerge with respect to their cellular function, regulation and utility as therapeutic targets.

• Initial Sequencing and Tissue Distribution of Toll-like Receptor 3 mRNA in White-tailed Deer (Odocoileus virginianus).

  Authors: Seychelle M Vos, Michael J Yabsley, Elizabeth W Howerth

  Journal of wildlife diseases. 08/2009; 45(3):785-90.

  Toll-like receptor (TLR) 3 recognizes double-stranded RNA (dsRNA) and activates a signal transduction pathway that results in the release of a variety of chemokines and cytokines and apoptoticToll-like receptor (TLR) 3 recognizes double-stranded RNA (dsRNA) and activates a signal transduction pathway that results in the release of a variety of chemokines and cytokines and apoptotic activity. Variability in TLR3 expression may play an important role in disease susceptibility of white-tailed deer (WTD; Odocoileus virginianus) to bluetongue and epizootic hemorrhagic disease viruses, which are dsRNA viruses. Because little is known about TLR3 in WTD, our objective was to sequence WTD TLR3 mRNA and to determine baseline levels of tissue expression. A 209-base pair sequence of TLR3 mRNA was obtained from WTD peripheral blood mononuclear cells. Dot blots confirmed that the sequence obtained was part of total WTD mRNA. Variable expression or ligand binding of TLR3 may contribute to observed susceptibility differences between populations of WTD, so the level of TLR3 in small intestine, skin, spleen, heart, cecum, rumen, lymph node, lung, kidney, and liver from WTD fawns (n=2) was analyzed using real-time reverse transcriptase-polymerase chain reaction. Tissue expression of TLR3 mRNA relative to the housekeeping gene beta-actin was highest in spleen, heart, skin, and lung.