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Publications (3)5.96 Total impact

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    ABSTRACT: Different fetal cell types have been found in the maternal blood during pregnancy in the past, but fetal cells are scarce, and the proportions of the different cell types are unclear. The objective of the present study was to identify specific fetal cell markers from fetal cells found in the maternal blood circulation at the end of the first trimester. Twenty-three fetal cells were isolated from maternal blood by removing the red blood cells by lysis or combining this with removal of large proportions of maternal white blood cells by magnetic-activated cell sorting. Fetal cells identified by XY fluorescence in situ hybridization and confirmed by reverse-color fluorescence in situ hybridization were shot off microscope slides by laser capture microdissection. The expression pattern of a subset of expressed genes was compared between fetal cells and maternal blood cells using stem cell microarray analysis. Twenty-eight genes were identified as fetal cell marker candidates. Of the 28 fetal marker candidate genes, five coded for proteins, which are located on the outer surface of the cell membrane and not expressed in blood. The protein product of these five genes, MMP14, MCAM, KCNQ4, CLDN6, and F3, may be used as markers for fetal cell enrichment.
    Prenatal Diagnosis 05/2012; 32(8):742-51. · 2.68 Impact Factor
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    Iben Sundtoft, Steffen Sommer, Niels Uldbjerg
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    ABSTRACT: Cervical collagen concentration decreases during pregnancy. The increased risk of preterm birth after a short interpregnancy interval may be explained by an incomplete remodeling of the cervix. The objective of this study was to describe the changes in cervical collagen concentration over 15 months after delivery. The collagen concentrations were determined in cervical biopsy specimens that were obtained from 15 women at 3, 6, 9, 12, and 15 months after delivery. The mean cervical collagen concentrations were 50%, 59%, 63%, 65%, and 65% of dry weight (SD, 4.2-6.5). This increase was statistically significant until month 9, but not between months 9 and 12. Low collagen concentrations in the uterine cervix may contribute to the association between a short interpregnancy interval and preterm birth.
    American journal of obstetrics and gynecology 07/2011; 205(1):59.e1-3. · 3.28 Impact Factor
  • Journal of Reproductive Immunology - J REPROD IMMUNOL. 01/2011; 90(2):170-170.