Ning Zhang

Publications (2)3.88 Total impact

• Article: Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth.

  Lili Liu, Ning Zhang, Jiayu Liu, Jie Min, Ningqiang Ma, Na Liu, Yi Liu, Helong Zhang
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  ABSTRACT: Aneuploid is recognized as a hallmark of cancer and is caused by chromosome mis-segregation during mitosis. Recent studies have identified shugoshin (SGO-1) as an important player in proper chromosome segregation and the involvement in tumorigenesis. But, little is known about how SGO-1 is involved. The aim of this study was to obtain information about the role of SGO-1 in human lung cancer cells. In our study, real-time PCR and western blotting assay were performed to detect the expression of SGO-1 in nonsmall cell lung cancer (NSCLC) cell lines. Then, we successfully constructed a lentivirus vector mediating RNAi targeting of SGO-1 (LV-SGO-1 siRNA) and proved that it can suppress the expression of SGO-1 gene in H1299 cells. We found that SGO-1 was highly expressed in NSCLC cancer cell lines. RNA interference of SGO-1 by the LV-SGO-1 siRNA construct significantly decreased SGO-1 protein expression and inhibited the growth and ability of forming colonies of H1299 cells with more cells arrested in G2/M phase, but lentivirus vector control had no effect on H1299 cells. Moreover, suppression of SGO-1 by LV-SGO-1 siRNA increased the apoptosis of H1299 cells with down-regulated Bcl-2 and up-regulated Bax. SGO-1 may provide a novel therapeutic target for the treatment of lung cancer since it inhibits the growth and increases the apoptosis of lung cancer cells.
  Tumor Biology 12/2011; 33(2):515-21. · 1.94 Impact Factor
• Article: Lentivirus-delivered Krüppel-like factor 8 small interfering RNA inhibits gastric cancer cell growth in vitro and in vivo.

  Lili Liu, Na Liu, Min Xu, Yi Liu, Jie Min, Hailin Pang, Ning Zhang, Hongbo Zhang, Helong Zhang
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  ABSTRACT: We found that the transcription factor Krüppel-like factor 8 (KLF8) was highly expressed in gastric cancer tissues and cell lines compared with adjacent noncancerous regions and gastric epithelial mucosa cells. We employed a lentivirus-mediated RNAi technique to knockdown KLF8 expression in gastric cancer cell line SGC7901 and observed its effects on cell growth in vitro and in vivo. Knockdown of KLF8 inhibited SGC7901 cell proliferation, promoted cell apoptosis, inhibited the tumorigenicity of SGC7901 cells, and significantly decreased tumor growth when the cells were injected into nude mice. These results indicated that overexpression of KLF8 may influence the biological behavior of SGC7901 gastric cancer cells. Knockdown of KLF8 expression by lentivirus-delivered siRNA may be useful as a therapeutic agent for the treatment of gastric cancer.
  Tumor Biology 11/2011; 33(1):53-61. · 1.94 Impact Factor