Qiong Yang

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (37)316.74 Total impact

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    ABSTRACT: A new fluorescent nanoparticle (PIOT-HA) is synthesized with cationic polyester (PIOT) and anionic hyaluronic acid (HA) by electrostatic interactions in an aqueous solution. The nanoparticles (NPs) are degradable upon treatments with alkali or hyaluronidase, which exhibits better biological safety and potential application in vitro and in vivo. Through specific interactions between the HA locating on the surfaces of PIOT-HA NPs and the CD44 protein over-expressed on the MDA-MB-231 cancer cell line, PIOT-HA NPs could selectively image the cancer cells. Upon white light irradiation, the PIOT-HA NPs can sensitize oxygen to generate reactive oxygen species (ROS) that inactivate the neighboring CD44 protein, which inhibits the migration of MDA-MB-231 cancer cells.
    Macromolecular Rapid Communications 03/2013; · 4.61 Impact Factor
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    ABSTRACT: A water soluble polythiophene with tyrosine kinase inhibitor lapatinib as side chain moieties (PTL) was designed and synthesized. Together with its fluorescent characteristic and low cytotoxicity, PTL can be used for cell membrane imaging of living cells by targeting the intracellular domain of transmembrane proteins.
    Polym. Chem. 03/2013;
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    ABSTRACT: A dopamine-modified conjugated polymer PFPDA is synthesized and characterized. At low pH, dopamine exists in its hydroquinone form and lacks the ability to quench fluorescence. At high pH, the proportion of the quinone form of dopamine increases due to its autooxidation, and efficient intramolecular electron transfer from the polymer main chain to quinone occurs, resulting in the quenching of the fluorescence of PFPDA. Thus, PFPDA exhibits a fluorescence “turn-on” response at low pH. PFPDA possesses excellent photostability and exhibits no cytotoxicity, which makes it a good fluorescent material for pH sensing and cell imaging. A light-induced hydroxyl anion emitter, MGCB, is also used to change the pH of the solution and thus regulate the fluorescence of PFPDA via remote control under light irradiation. Because the cytoplasm becomes acidic when cell autophagy occurs, PFPDA can also be used for autophagy imaging of HeLa cells with good selectivity.
    Advanced Functional Materials 02/2013; 23(6). · 10.44 Impact Factor
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    ABSTRACT: Multifunctional cationic conjugated polyelectrolyte (CP)-coated bacteria are fabricated for drug loading, delivery and release with multimodal anticancer activity. Facilitated by the collaborative release effect caused by CP and an antibiotic polymyxin B, the loaded toxins leak out from the vectors in considerable amounts and lead to tumor cell death. In addition, the CP coated bacterial vectors can sentisize ROS, which favors light-mediated tumor cell killing.
    Advanced Materials 12/2012; · 15.41 Impact Factor
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    ABSTRACT: A new bifunctional cationic conjugated polyfluorene derivative (PFPBOH) containing phenylboronic acid group has been synthesized and characterized. The phenylfluorenyl backbone and positively charged groups in the side chain give the polymer PFPBOH excellent fluorescence property and good water solubility. Phenylboronic acid moieties on the side chain of the polymer form cyclic esters with adjacent diols on cell membrane, which can be employed in cell imaging. Besides that, the fluorescence of PFPBOH can be quenched by p-nitroaniline released via enzyme reaction, thus, the polymer can be used for γ-glutamyltranspeptidase detection through a fluorescence “turn off” mechanism. These findings exhibit great potential for developing multifunctional polymer materials for simultaneous imaging and detection.
    Macromolecular Chemistry and Physics 12/2012; 213(23). · 2.45 Impact Factor
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    ABSTRACT: Multifunctional nanomaterials with simultaneous therapeutic and imaging functions explore new strategies for the treatment of various diseases. Conjugated polymers (CPs) are considered as novel candidates to serve as multifunctional delivery systems due to their high fluorescence quantum yield, good photostability, and low cytotoxicity. Highly sensitive sensing and imaging properties of CPs are well reviewed, while the applications of CPs as delivery systems are rarely covered. This feature article mainly focuses on CP-based multifunctional non-viral delivery systems for drug, protein, gene, and cell delivery. Promising directions for the further development of CP-based delivery systems are also discussed.
    Macromolecular Bioscience 11/2012; · 3.65 Impact Factor
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    ABSTRACT: Alterations in the methylation of promoters of cancer-related genes are promising biomarkers for the early detection of disease. Compared with single methylation alteration, assessing combined methylation alterations can provide higher association with specific cancer. Here we use cationic conjugated polymer-based fluorescence resonance energy transfer to quantitatively analyse DNA methylation levels of seven colon cancer-related genes in a Chinese population. Through a stepwise discriminant analysis and cumulative detection of methylation alterations, we acquire high accuracy and sensitivity for colon cancer detection (86.3 and 86.7%) and for differential diagnosis (97.5 and 94%). Moreover, we identify a correlation between the CpG island methylator phenotype and clinically important parameters in patients with colon cancer. The cumulative analysis of promoter methylation alterations by the cationic conjugated polymer-based fluorescence resonance energy transfer may be useful for the screening and differential diagnosis of patients with colon cancer, and for performing clinical correlation analyses.
    Nature Communications 11/2012; 3:1206. · 10.74 Impact Factor
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    ABSTRACT: For most molecule-targeted anticancer systems, intracellular protein targets are very difficult to be accessed by antibodies, and also most efforts are made to inhibit protein activity temporarily rather than inactivate them permanently. In this work we firstly designed and synthesized multifunctional polymer-drug conjugates (polythiophene-tamoxifen) for intracellular molecule-targeted binding and inactivation of protein (estrogen receptor α, ERα) for growth inhibition of MCF-7 cancer cells. Small molecule drug was conjugated to polymer side chain for intracellular signal protein targeting, and simultaneously the fluorescent characteristic of polymer for tracing the cellular uptake and localization of polythiophene-drug conjugates by cell imaging. Under light irradiation, the conjugated polymer can sensitize oxygen to produce reactive oxygen species (ROS) that specifically inactivate the targeted protein, and thus inhibit the growth of tumor cells. The conjugates showed selective growth inhibition of ERα positive cancer cells, which exhibits low side effect for our intracellular molecule-targeted therapy system.
    Scientific Reports 10/2012; 2:766. · 5.08 Impact Factor
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    ABSTRACT: An intrinsically fluorescent cationic polyfluorene (CCP) has been designed, synthesized, characterized, and examined as a plasmid DNA (pDNA) delivery vector. This material facilitates nucleic acid binding, encapsulation and efficient cellular uptake. CCP can effectively protect pDNA against nuclease degradation, which is necessary for gene carriers. Green fluorescent protein (GFP) expression experiments reveal that CCP can achieve efficient delivery and transfection of pDNA encoding GFP gene with 92% efficiency, which surpasses that of commercial transfection agents, lipofectamine 2000 (Lipo) and polyethylenimine (PEI). CCP is also highly fluorescent, with 43% quantum yield in water, and exhibits excellent photostability, which allows for real-time tracking the location of gene delivery and transfection. These features and capabilities represent a major step toward designing and applying conjugated polymers that function in both imaging and therapeutic applications.
    Advanced Materials 08/2012; 24(40):5428-32. · 15.41 Impact Factor
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    ABSTRACT: Except for chemotherapy, surgery, and radiotherapy, photodynamic therapy (PDT) as new therapy modality is already in wide clinic use for the treatment of various diseases. The major bottleneck of this technique is the requirement of outer light source, which always limits effective application of PDT to the lesions in deeper tissue. Here, we first report a new modality for treating cancer and microbial infections, which is activated by chemical molecules instead of outer light irradiation. In this system, in situ bioluminescence of luminol can be absorbed by a cationic oligo(p-phenylene vinylene) (OPV) that acts as the photosensitizer through bioluminescence resonance energy transfer (BRET) process. The excited OPV sensitizes oxygen molecule in the surroundings to produce reactive oxygen species (ROS) that kill the adjacent cancer cells in vitro and in vivo, and pathogenic microbes. By avoiding the use of light irradiation, this work opens a new therapy modality to tumor and pathogen infections.
    Journal of the American Chemical Society 07/2012; 134(32):13184-7. · 11.44 Impact Factor
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    ABSTRACT: A new system was developed for sensitive and selective detection of tumor cells taking advantage of cell-attached aptamers amplified by PCR and output signals amplified by cationic conjugated polymers.
    Chemical Communications 06/2012; 48(60):7465-7. · 6.38 Impact Factor
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    ABSTRACT: A visual colorimetric method for detecting multiplex DNA mutations has been developed using multicolor fluorescent coding based on multistep FRET of cationic conjugated polymers. Expensive instruments and technical expertise are not required in this method. Also our visual system provides a quantitative detection by simply analyzing RGB values of images. Genomic DNAs extracted from 60 FFPE colon tissues can be sensitively determined by utilizing our visual assay with a high-throughput manner. Thus, it proves to be sensitive, reliable, cost-effective, simple, and high-throughput for mutation detection.
    ACS Applied Materials & Interfaces 06/2012; · 5.90 Impact Factor
  • Chemical Reviews 06/2012; 112(8):4687-735. · 45.66 Impact Factor
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    ABSTRACT: Three cationic polythiophene derivatives (P1, P2, P3) were synthesized and characterized. Under white light irradiation (400-800 nm), they sensitize oxygen molecule in the surrounding to generate reactive oxygen species (ROS) that can efficiently unwind the supercoiled DNA in vitro. Further study shows that this relaxation of the DNA supercoiling results in the decrease of gene (pCX-EGFP plasmid) expression level. The ability of these conjugated polymers for regulating gene expression will add a new dimension to the function of conjugated polymers.
    ACS Applied Materials & Interfaces 04/2012; 4(5):2334-7. · 5.90 Impact Factor
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    ABSTRACT: The mutation detections of KRAS and BRAF genes are of significant importance to predict the responses to anti-cancer therapy and develop new drugs. In this paper, we developed a multi-step fluorescence resonance energy transfer (FRET) assay for multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers (CCP). The newly established detection system could detect as low as 2% mutant DNAs in DNA admixtures. By triggering the emission intensity change of CCP and the dyes labeled in the DNA, four possible statuses (three mutations and one wildtype) can be differentiated in one extension reaction. The detection efficiency of this new method in clinical molecular diagnosis was validated by determining KRAS and BRAF mutations of 51 formalin-fixed paraffin-embedded (FFPE) ovary tissue samples. Furthermore, the result of the CCP-based multi-step FRET assay can be directly visualized under UV light so that no expensive instruments and technical expertise are needed. Thus, the assay provides a sensitive, reliable, cost-effective and simple method for the detection of disease-related gene mutations.
    Chinese Science Bulletin 03/2012; 58(8). · 1.37 Impact Factor
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    ABSTRACT: At present, there is an urgent necessity for the discovery of new chemotherapeutic agents with novel molecular skeleton structures that exhibit wide spectrum antitumor activity. In this work, a cationic pentathiophene (5T) is synthesized and discovered to have both anticancer activity and molecular imaging property. 5T can selectively accumulate in mitochondria to exhibit organellar imaging and efficiently induce cell apoptosis associating with JNK pathway activation. Additionally, complexes are prepared through electrostatic interactions between 5T and sodium chlorambucil (a widely used anticancer drug) with varying molar ratios. The complexes form nanoparticles in water with the size of about 50 nm. The 5T-chlorambucil nanoparticles enhance anticancer activity by 2–9 fold due to the synergistical anticancer activity of 5T and chlorambucil. 5T is therefore a promising multifunctional anticancer agent that incorporates optical monitoring capability and anticancer activity that targets mitochondria.
    Advanced Functional Materials 02/2012; 22(4). · 10.44 Impact Factor
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    ABSTRACT: A cationic polythiophene-porphyrin (PTP) dyad is shown to exhibit efficient light-activated antifungal activity. Higher singlet oxygen (¹O₂) generation efficiency can be attained from PTP upon photoexcitation due to the light-harvesting properties of the polymer backbone and efficient energy transfer from the polythiophene to the porphyrin units. PTP can be used for treating fungal infections in lower doses of irradiation light and polymer concentration.
    Small 02/2012; 8(4):524-9. · 7.51 Impact Factor
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    ABSTRACT: The recent emergence of antibioticresistant micro-organisms has been a public-health problem world wide. A cationic polythiophene-porphyrin dyad (PTP) is shown to exhibit efficient lightactivated antifungal activity. The positive charges and amphiphilic structure of PTP encourage its adsorption to the fungus cell surface based on electrostatic and hydrophobic interactions. Higher efficiency in singlet oxygen (1O2) generation can be attained from PTP upon photoexcitation due to the light-harvesting properties of the polymer backbone and efficient energy transfer from the polythiophene backbone to the porphyrin units. PTP can be used for treating fungal infections at low doses of irradiation light and polymer concentration.
    Small 02/2012; 8(4):524. · 7.51 Impact Factor
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    ABSTRACT: Multifunctional materials that simultaneously provide therapeutic action and image the results provide new strategies for the treatment of various diseases. Here, it is shown that water soluble conjugated polymers with a molecular design centered on the polythiophene−porphyrin dyad are effective for killing neighboring cells. Following photoexcitation, energy is efficiently transferred from the polythiophene backbone to the porphyrin units, which readily produce singlet oxygen (1O2) that is toxic for the cells. Due to the light-harvesting ability of the electronically delocalized backbone and the efficient energy transfer amongst optical partners, the polythiophene−porphyrin dyad shows a higher 1O2 generation efficiency than a small molecule analog. The fluorescent properties of these polymers can also serve to distinguish amongst living and dead cells. Polymers can be designed with folic acid grafted onto the polymer side chain that can specifically kill folate receptor-overexpressed cells, thereby providing an important demonstration of anticancer specificity through molecular design.
    Advanced Functional Materials 11/2011; 21(21). · 10.44 Impact Factor
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    ABSTRACT: A new water-soluble conjugated polymer containing fluorene and boron-dipyrromethene repeat units in the backbones (PBF) that exhibits red emission was synthesized and characterized. Cationic PBF forms uniform nanoparticles with negatively charged disodium salt 3,3'-dithiodipropionic acid (SDPA) in aqueous solution through electrostatic interactions. The nanoparticles display absorption maximum at 550 nm and emission maximum at 590 nm. Upon photoexcitation with white light (400-800 nm) with 90 and 45 mW·cm(-2) for bacteria and cancer cells killing respectively, PBF nanoparticles can sensitize the oxygen molecule to readily produce reactive oxygen species (ROS) for rapidly killing neighboring bacteria and cancer cells. Furthermore, PBF nanoparticles concurrently provide optical imaging capability. PBF nanoparticles are therefore a promising multifunctional material for treating cancers and bacteria infections, while concurrently providing optical monitoring capabilities.
    Langmuir 11/2011; 28(4):2091-8. · 4.38 Impact Factor

Publication Stats

251 Citations
316.74 Total Impact Points


  • 2011–2013
    • Chinese Academy of Sciences
      • Key Laboratory of Organic Solids
      Peping, Beijing, China
  • 2010–2011
    • Northeast Institute of Geography and Agroecology
      • Key Laboratory of Organic Solids
      Beijing, Beijing Shi, China